The objective of this study was to compare of different isolation method of mouse preantral follicles, and to examine in vitro development of mouse preantral follicles isolated by different method. Preantral follicles were mechanically or enzymatically extracted from mouse ovaries. Mechanical isolation method used fine gauge needles and enzymatic method of isolating follicles used collagenase. The recovered preantral follicles were cultured for 10 days in alpha-minimal essential medium (-MEM) + 5% FBS + Insulin-Transferrin-Selenium (ITS) + 100 mIU/ml FSH. The collected primary follicles by enzymatic treatment were higher than mechanical method. Others stage preantral follicle by mechanical isolation were higher than enzymatic method. After 10 days of culture, no statistical differences were shown in survival rates of preantral follicle among the 2 culture groups. The metaphase II rates of the oocytes were significantly higher (p<0.05) in mechanical method (17.8%) than in enzymatic method (5.1%). These results suggest that the isolation method of choice depends on the target stage preantral follicles and mechanical isolation is an optimal method of preantral folliclesin a culture of mouse preantral follicle.

언양읍성 북문주변 성곽정비사업 일환으로 발굴조사한 지역에서 체성, 북문지와 옹성 1개소, 치성 2개소, 성 외측에 전체적으로 조성된 해자, 수혈유구 등 다양한 유구가 조사되었다. 이번 발굴조사에서 북문지와 옹성의 구조, 체성부와 치성의 축조방법, 규모 등이 명확하게 확인되었다. 특히 체성의 축조방법에 있어서 내벽의 경우 계단식 축조수법이 아닌 내탁부와 성 안쪽과 내벽 내탁부를 구분하는 경계석열이 확인된 것이 가장 큰 성과이다. 해자의 평면형태, 규모, 내부시설을 정확하게 확인할 수 있었으며, 또한 체성, 치성, 옹성과의 축조에 있어서 선후관계를 파악할 수 있었다. 즉 체성과 치성은 동시기에 축조되었으며, 북문지의 옹성은 체성보다 늦은 시기에 축조된 것으로 확인되었다. 조사지역에서 해발고도가 가장 높은 북서쪽 모서리에서 암거시설 형태의 입수구가 확인되었다. 그리고 해자와 만나는 구상유구가 확인되었는데, 입지조건, 내부시설 등으로 볼 때 해자쪽으로 물을 공급하는 용도의 구로 생각된다. 언양읍성은 문헌에서 확인된 읍성(석성)의 축조시기(1500년)와 이번 발굴조사에서 출토된 유물(자기류, 기와류)의 편년을 비교해 볼 때 연대가 비교적 일치하는 것으로 생각되며, 이번 발굴조사 성과를 바탕으로 북문주변 성곽 정비복원 시에 문헌자료와 함께 고고학 자료를 검토, 보완하여 정확한 정비복원이 이루어지길 바란다.

The purpose of the study was to evaluation of the radiation dose reduction using various automatic exposure control (AEC) systems in different manufactures multi-detector computed tomography (MDCT). We used three different manufacturers for the study: General Electric Healthcare, Philips Medical systems and Siemens Medical Solutions. The general scanning protocol was created for the each examination with the same scanning parameters as many as possible. In the various AEC systems, the evaluation of reduced-dose was evaluated by comparing to fixed mAs with using body phantom. Finally, when we applied to AEC for three manufacturers, the radiation dose reduction decreased each 35.3% in the GE, 58.2% in the Philips, and 48.6% in the Siemens. This applies to variety of the AEC systems which will be very useful to reduce the dose and to maintain the high quality.

전통적인 Demerit 관리도는 일반적으로 자동차, 컴퓨터, 핸드폰 등 하나의 복잡한 제품에서 다양한 유형의 결점들이 동시에 발생하는 공정을 모니터링하기 위하여 사용된다. 관리도에서 공정 평균의 작은 변동을 빠르게 감지하기 위한 방법으로 EWMA기법은 매우 효과적이다. 본 연구에서는 EWMA 기법 보다 공정 평균의 작은 변동에 더 좋은 성능을 보이는 GWMA기법과 전통적인 Demerit 관리도(1928)를 결합한 Demerit-GWMA(generally weighted moving average) 관리도를 제안하였으며 그 관리도의 수행도를 평가하였다. 그 수행도 평가 결과, Demerit-GWMA 관리도는 공정 평균의 작은 변동을 감지하는데 classical Demerit 관리도, Demerit-EWMA. 관리도에 비하여 더 민감하다.

Culture of preantral follicles has important biotechnological implications through its potential to produce large quantities of oocytes for embryo production and transfer. The objective of this study was to determine the comparison of different isolation method of mouse preantral follicles, and to examine in vitro development of mouse preantral follicles isolated by different method. Preantral follicles were mechanically or enzymatically extracted from mouse ovaries. Mechanical isolation method used fine gauge needles and enzymatic method of isolating follicles used 1 mg/ml collagenage (Type IA) and 0.2 mg/ml DNase Ⅰ in Leibovitz L-15 medium. The solution containing Leibovitz L-15 medium, enzyme and ovary fragments was incubated at 37℃ for 30 min. The selection criteria are as follows: primary follicle of 75 to 99 μm, early secondary follicle of 100 to 125 μm and late secondary follicle of 126 to 150 μm in diameter. The recovered preantral follicles were cultured for 10 days in alpha-minimal essential medium (α-MEM) + 5% FBS + ITS + 100 mIU/ ml FSH. The collected primary follicles by enzymatic treatment were higher than mechanical method. Others stage preantral follicle by mechanical isolation were higher than enzymatic method. After 10 days of culture, no statistical differences were shown in survival rates of preantral follicle among the 2 culture groups. The metaphase Ⅱ rates of the oocytes were significantly higher (p<0.05) in mechanical method (17.8%) than in enzymatic method (5.1%). These results suggest that the isolation method of choice depends on the target stage preantral follicles and mechanical isolation is an optimal method of preantral follicles in a culture of mouse preantral follicle.

The CdS thin film used as a window layer in the CdTe thin film solar cell transports photo-generated electrons to the front contact and forms a p-n junction with the CdTe layer. This is why the electrical, optical, and surface properties of the CdS thin film influence the efficiency of the CdTe thin film solar cell. When CdTe thin film solar cells are fabricated, a heat treatment is done to improve the qualities of the CdS thin films. Of the many types of heat treatments, the CdCl2 heat treatment is most widely used because the grain size in CdS thin films increases and interdiffusion between the CdS and the CdTe layer is prevented by the heat treatment. To investigate the changes in the electrical, optical, and surface properties and the crystallinity of the CdS thin films due to heat treatment, CdS thin films were deposited on FTO/glass substrates by the rf magnetron sputtering technique, and then a CdCl2 heat treatment was carried out. After the CdCl2 heat treatment, the clustershaped grains in the CdS thin film increased in size and their boundaries became faint. XRD results show that the crystallinity improved and the crystalline size increased from 15 to 42 nm. The resistivity of the CdS single layer decreased from 3.87 to 0.26 Ωcm, and the transmittance in the visible region increased from 64% to 74%.

Supply chain for the economic development of East Asian economic development model, the study's purpose is to establish. Korea, Japan, China, Russia and Asia, including North Korea and get the status of economic development by focusing on key issues and proposed solutions, within a few years of the coming of a new East Asian economic integration of new supply chain would like to prepare for the era. Weight The weight of the center to the center of East Asia, looking for techniques to approach the supply chain scenario, the economic development strategies and measures were studied. East Asia's economic potential value of the supply chain, identify and recognize the importance and benefits should be Presents detailed country-specific development strategies and leading Asian economies will have to pay the supply chain. Republic of Korea's leading East Asian economies in order to supply the industry, industry development strategy as detailed in the running to move. North and South Korea for economic integration, the era of supply-chain economy, you need to prepare calmly.

Coloured wastewater is released as a direct result of the production of dyes as well as from various other chemical industries. Many dyes and their breakdown products may be toxic for living organisms. Activated carbon is one of the best materials for removal of dyes from aqueous solutions. The present study describes the adsorption behaviour of methylene blue dye on three microporous activated carbons, where two samples (AC-1 and AC-2) were prepared by a polymer blend technique and the other is a microporous activated carbon (ARY-3) sample from viscose rayon yarn prepared by chemical-physical activation. The effects of contact time and activated carbon dosage on decolourisation capacity have been studied. The results show that activated carbon having mixed microporosity and mesoporosity show tremendous decolourisation capacity for methylene blue. In addition, the activated carbon in the powder form prepared by the polymer blend technique shows better decolourisation capacity for methylene blue than the activated rayon yarn sample.

Since 'The Act on Private Investment of The Infrastructure' was established in 1994, private investment as well as government's investment in transport infrastructure has been active. However investment in transport infrastructure has more risks than others' due to uncertainty both in traffic volume and in construction cost. In the current appraisal procedure of deciding transportation infrastructure investment, instead of risk management, the sensitivity analysis considering only the changes of benefit, cost and social discount rate which are main factor affecting economic feasibility is carried out. Therefore the uncertainty of various factors affecting demand, cost and benefit are not considered in feasibility study. In this study the problems in current investment appraisal system were reviewed. Using Delphi technique the major factors which have high uncertainty in feasibility study were surveyed and then improvement plan was suggested in the respective of classic 4 step demand forecasting method. The range estimation technique was also mentioned to deal with the uncertainty of the future.

케이블 구조물은 비교적 가볍고 넓은 공간의 형성이 쉬우나 유연한 특징으로 인해 형상의 제어가 매우 민감하다. 이런 구조물은 여러 이유에서 형상의 보정이 필요하며, 특히 부재 제어량과 어떠한 부재를 제어해야 하는가는 것은 많은 연구자들이 고심하고 있는 문제이다. 따라서 본 논문의 목적은 하중법(Force Method)를 이용해서 형상조절을 위한 변위 제어기법을 연구하는 것이다. 논문은 2장에서는 제어 방정식을 설명하고, 3장에서는 단순 케이블 넷 모델을 이용하여 동시 및 순차제어를 고려해 해석을 수행하고 결과를 고찰한다. 4장에서는 보다 더 복잡한 케이블 돔 구조물에 적용하여 가장 유용한 부재에 대해서 논의 하고, 5장에서 결과를 요약한다.

The purpose of this study was to minimize of entrance surface dose (ESD) at the eye using high kVp technique in the computed radiography. We used REX-650R (Listem, Korea) general X-ray unit, and external detector with ESD dosimeter of Piranha 657 (RTI Electronics, Sweden). We used head of the whole body phantom. The total 64 images of X-ray anterior-posterior of skull were acquired using the film/screen (F/S) method and the digital of computed radiography method. The three radiology professor of more 10 years of clinical career evaluated a X-rays images in the same space by 5-point scale. The external detector was performed measurement of ESD of three times by same condition on the eye of the head phantom. The good image quality in the F/S method (90 kVp, 2.5 mAs) showed at the minimized ESD of 0.310 ± 0.001 mGy. the good image quality in the computed radiography method (90 kVp, 2.0 mAs) showed at the minimized ESD of 0.180 ± 0.002 mGy (P = 0.002). Finally the radiation dose could reduced about 50% in the computed radiography method more than the F/S method. In addition the eye entrance surface dose using high kVp technique with the computed radiography was reduced 92% more than conventional technique (F/S method).

부산광역시 기념물 제 40호로 지정되어 있는 기장읍성은 조선시대의 읍성축조수법이 정형화된 형식으로 정리되기 이전에 축조되어 고려시대 성곽 축조수법과 조선의 읍성축조수법이 혼합되어 성곽축성사에 있어 읍성축조수법의 변화를 파악할 수 있다는 점에서 그 가치가 있다고 할 수 있겠다. 기장읍성에서 확인되는 사직선기단의 축조는 과거 고려시대말까지 계속 축조된 판축토성(版築土城)의 기단부 축조방식을 읍성축조에 적용한 결과로 고려 판축토성에서 조선시대 석축성인 연해읍성의 축조로 이어지는 흐름의 연결고리라고 할 수 있다. 기장읍성 동벽 체성부에서 확인되는 세장방형 외벽면석 축조수법은 일반적으로 알려진 조선시대 읍성의 장대석 입수적 축조수법과는 차별성을 지닌다. 기장읍성 남벽과 동벽 체성부 기저부 축조수법은 김해읍성, 웅천읍성, 동래읍성, 고성읍성 등에서 확인되는 지대석 설치 이전에 바닥에 판석을 이용하여 바닥면의 수평을 맞추어 정리된 구조물은 확인되지 않는다. 따라서 이러한 축조수법이 사용되기 이전 시기에 축조된 형태임을 알 수 있다. 이를 통해 기장읍성은 사직선기단축조수법, 체성부 세장방형 외벽면석의 사용과 더불어 고려시대의 성곽축조수법과 조선시대 연해읍성 축조수법이 혼용된 과도기적 읍성임을 알 수 있는 것이다.

The aim of this study was to determine the immediate effects of single treatment of strain-counter strain (SCS) on pressure pain threshold (PPT) and muscle activity during scapular plane abduction with 3% body weight load. Fifteen asymptomatic male adults with upper trapezius latent trigger point (LTrP) (PPT<2.9 ) participated in this study. Pressure algometer was used to measure PPT and surface electromyography was used to record upper, middle arid lower trapezius, serratus anterior, infraspinatus and middle deltoid muscle activity and relative ratio during scapular plane abduction between pre- and post-intervention. There was a significant increase in upper trapezius PPT after a 90-second SCS (p<.05). The activity of the upper trapezius and middle deltoid was significantly decreased (p=.014, p=.001), coupled with a decreased muscle activity ratio between the upper and lower trapezius (p<.05). These results indicate that the SCS may effectively deactivate upper trapezius activity, thereby alleviating muscle balance and reducing pain sensitivity.

One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures〔10% (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.〕which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into LN2. One-step dilution in straw was done in 25℃ water for 1 min, by placing vertically in the state of plugged- end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.