검색결과

검색조건
좁혀보기
검색필터
결과 내 재검색

간행물

    분야

      발행연도

      -

        검색결과 13,928

        2841.
        2015.09 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        The effects of coating parameters were investigated in wear resistance coatings of Diamalloy-406 on Inconel 718 to obtain an optimum coating condition by high velocity oxy-fuel spraying. The coating parameters, the flow rates of source gases (hydrogen and oxygen), the powder feed rate, and the spray distance, were designed by the Taguchi method. The optimal conditions were determined: oxygen flow rate 34 FRM, hydrogen flow rate 57 FRM, powder feed rate 35 g/min, and spray distance 7 inch. Friction coefficients of the coating and the substrate decreased with an increasing sliding surface temperature from 25 oC to 450 oC. The friction coefficient of Diamalloy-4006 coating decreased as the sliding surface temperature increased from 0.43 ± 0.01 at 25 oC to 0.29 ± 0.01 at 450 oC. The wear trace and wear depth of the coating were smaller than the substrate at all temperatures tested. The relationship between spray parameters and wear resistance was discussed extensively, based on the measured roughness, hardness, and porosity in each coating.
        4,000원
        2842.
        2015.09 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        The γ/γ´ two-phases, commonly known as a eutectic structure, are observed in the γ interdendritic region of a Nibase superalloy. However, the growth behavior of the γ/γ´ two-phases, whether it is of eutectic or peritectic nature, has not been decidedly established. Directional solidifications were, thus, performed with the planar interface at a low growth rate of 0.5 μm/s in order to promote macro segregation. Directional solidification started with the γ planar interface and the γ´ phase nucleated on the γ planar interface at the solidification fraction of 0.75. The γ/γ´ two-phases showed the γ´ rod structure as major phase and the γ minor phase between γ´ rods, and the volume fraction of the γ phase changed continuously with an increasing solidification fraction. The two-phase γ/γ´ is seen as the coupled peritectic.
        4,000원
        2843.
        2015.09 KCI 등재 SCOPUS 구독 인증기관 무료, 개인회원 유료
        Resistance switching memory cells were fabricated using atomically dispersed Pt-SiO2 thin film prepared via RF co-sputtering. The memory cell can switch between a low-resistance-state and a high-resistance-state reversibly and reproducibly through applying alternate voltage polarities. Percolated conducting paths are the origin of the low-resistance-state, while trapping electrons in the negative U-center in the Pt-SiO2 interface cause the high-resistance-state. Intermediate resistance-states are obtained through controlling the compliance current, which can be applied to multi-level operation for high memory density. It is found that the resistance value is related to the capacitance of the memory cell: a 265-fold increase in resistance induces a 2.68-fold increase in capacitance. The exponential growth model of the conducting paths can explain the quantitative relationship of resistance-capacitance. The model states that the conducting path generated in the early stage requires a larger area than that generated in the last stage, which results in a larger decrease in the capacitance.
        4,000원
        2844.
        2015.09 KCI 등재 구독 인증기관·개인회원 무료
        CESL은 EU 역내 계약법을 개정하지 않고 개별회원국이 채택하는 경 우 특별법의 지위를 점할 수 있도록 성안되었다. 이에 CESL은 국제상사 계약에 있어 계약당사자의 합의에 따라 자발적으로 적용된다. CESL상 계약당사자의 의무 및 불이행에 관한 규정체계는 구체적이고도 광범위하 다. 주요 내용은 의사표시하자, 계약의 현저한 불균형 내지 불공정성, 표 준약관 삽입과 관련한 법적 유효성, 소유권 또는 담보권의 이전에 대한 법률효과, 계약의 유효성, 서비스 계약 및 디지털 콘텐츠와 관련한 계약 등이다. 이 경우 CESL은 서식의 교전에 관한 규정을 보유하여 보다 포 괄적이고 응집된 규칙을 보유하고 있는 특징이 있다. CESL은 독립적으로 그리고 당해 목적과 일반원칙에 따라 해석되어져 야 하고, 나아가 CESL의 범위 내에 있지만 명시적으로 규정하고 있지 않는 사안에 대하여도 그 처지는 마찬가지다. CESL의 적용에 대하여는 해석, 합리성, 불요식, 개별적으로 협상되지 않는 계약내용, 계약의 해제, 혼합목적의 계약, 통지, 기간의 계산, 일방적인 진술 또는 행동 등이다. CESL은 계약체결 전 일방이 계약내용에 기한 정보를 제공하지 않을 경우 신의 및 공정거래 위반에 해당되는 것으로 취급하고 있다. 나아가 원거리 계약의 경우 전자적 방법의 계약체결을 용인하고 있고 계약체결 전 또는 체결 시 정보를 제공하는 일방은 계약내용의 구속력 확보를 위 해 그리고 특정장소에서 당해 의무를 준수하기 위한 정보가 정확히 전달 되었음을 확인할 수 있도록 합리적 주의의무를 부과하고 있다. 만약 당 해 의무위반에 기하여 부정하거나 잘못된 정보가 제공된 경우 타방은 특 단의 구제권을 행사할 수 있다. CESL은 당사자의 합의, 합의에 기한 법적 구속력, 충분한 계약의 내 용과 확실성 등을 계약체결의 요건으로 두고 있다. 또한 의사표시의 하 자에 기하여 착오ㆍ사기ㆍ강박ㆍ부당한 착취에 대해 계약을 취소할 수 있는 권리를 보장하고 있으며, 취소통지, 취소효과, 추인, 손해배상금, 구 제수단의 배제 또는 제안과 구제수단의 선택 등을 이에 추보하고 있다. 나아가 계약내용의 해석기준으로서 계약내용 해석의 원칙, 계약내용 및 그 효력, 불공정한 계약내용 등으로 구분하여 다루고 있는 특징이 있다.
        2845.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The objective of this study is to investigate the effect of ELF (Extremely Low Frequency) exposure on the health of elementary school students. A total of 103 students 12~13 years old were chosen for the study. The experimental group consisted of 56 students who went to school near an overhead powerline. The ELF exposure and environmental hazard factors were evaluated during a 24-hour exposure period. The body and dwelling characteristics of the students as well as disease occurrence related to the respiratory system and allergies were investigated through a questionnaire. The brain wave and electrocardiogram were also inspected. The exposure of the group exposed while at a school located near a powerline was 6.8 mG (p<0.01). Based on the questionnaire results, neither the body or dwelling characteristics of the two groups were affected by the ELF emitted from powerline. There was no significant difference between the two groups in relation to the rate of occurrence of respiratory diseases such as wheezing, asthma and bronchitis. Although brain waves of the group exposed to ELF were lower than that of the group not exposed to it in terms of absolute power of gamma and beta, there is no significant difference between the health status of the two groups. According to our study, school students who lived near a power line were exposed to higher ELF levels than those away from a power line. The two groups have significant differences inBrain wave and ECG, but this change doesn't mean there is a difference in health status. Finally, our study has a limitation in terms of the number of study subjects and the restricted area examined.
        4,000원
        2846.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was conducted to evaluate the quality of indoor air and health-related parameters (allergic rhinoconjunctivitis) of subject of study (control group and sensitive group) in two schools. The schools were categorized into two groups of newly-built school and the school older than 5 years. Removal of indoor pollutant was investigated according to volume rates (0%, 1.5%, 3%) of indoor plants inside 3 classrooms respectively. The chemicals of indoor environmental research were PM10, Volatile organic compounds (toluene and benzene), formaldehyde, temperature and humidity. ARIA (Allergic rhinitis impacts on Asthma) test was used to assess health effect for 151 students. Also, The variation of SBS symptoms for students in classroom was measured by intervention. With increasing volume ratio of classrooms, there were positive and significant results between the indoor pollutant and student's health score. Students showed improvement health score during the period of putting indoor plants, which was facilitated by the placement of indoor plants at newly-built school classroom of indoor plants volume ratio 3%. From the results above, it could be tentatively effective newly-built school classroom of indoor plants volume ratio 3% improve indoor air quality and student's health score.
        4,000원
        2847.
        2015.09 KCI 등재 구독 인증기관·개인회원 무료
        이 연구는 2012년 2월6일 정부가 발표한 학교폭력 근절 종합대책 의 내용을 검토해 보고 학교폭력 예방을 위한 학교 차원의 회복적 사법의 적용과 한국사회 학교폭력의 실태와 효율적 대처방안으로 학교 구성원을 포함한 사회적 공감대의 형성, 학교폭력 근절 종합대책의 실효적 보완을 통한 지속적인 실천, 보다 학교폭력해소를 위한 근본대책을 제시함을 목 적으로 한다. 학교폭력은 세계 각국의 공통적인 현상이라고 할 수 있으 며, 우리나라도 예외는 아니어서 학교폭력에 대한 예방책을 제시 및 시 행하고 있으나 근절에는 미치지 못하고 있는 실정이다. 오히려 학교폭력 은 점차 악화되고 있기까지 하다. 최근 학교폭력의 특징은 흉포화, 저연 령화, 강력화되어 그 심각성은 도를 넘고 있다. 특히 대구 중학생 자살사 건, 경산 고등학생 아파트 투신 자살사건, 대전 여고생 자살사건, 김해 여고생 폭행치사 사건 등의 일련의 자살사건의 원인에 학교폭력이 작용 했다는 것이 알려지면서 심각한 사회문제화 된 학교폭력-청소년비행에 적절한 대응이 이루어지지 못하고 있다는 자성의 목소리가 커지면서 이 에 대한 원인 규명에 대한 노력이 다각도로 이루어지고 있다. 이에 정부 는 학교폭력 피해학생을 실질적으로 보호하며 가해학생을 엄하게 처벌한 다는 대책을 발표했다. 현행 학교폭력 근절대책에서는 피해학생과 가해 학생을 분리시키고 가해학생에게는 출석정지, 강제 전학, 퇴학과 생활기 록부 기재 등이 추가되는 등 처벌을 강화시키고 있는 추세이다. 반면 학 교폭력은 예전과는 또 다른 양상으로 발전·진화하고 있다. 소수 특정학 생에서 일반학생까지 확대되고 있으며 신체적 폭력 외에도 정서적 폭력, 언어폭력, 사이버 폭력, 성추행 등으로 다양해지며 가해자와 피해자의 위 치가 쉽게 전환된다는 점이다. 따라서 본 연구에서는 이와 같이 나날이 진화·발전하는 학교폭력의 사전 예방 및 대처의 한 방법에 주목하여 학생, 가족, 학교 및 지역사회 의 다차원적 개입의 필요성과 피해회복, 피해자-가해자의 조정·화해 및 공동체 강화를 강조하는 회복적 사법이 학교차원에서 실천 적용될 필요 성을 제시하였다.
        2848.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The purpose of this study is to improve the ammonia (NH3) emission inventory estimation in the domestic CAPSS (Clean Air Policy Support System). Currently, the ammonia emissions rate is obtained by multiplying the activity (number of livestock) by emission factors. The amount of movement and treatment are not considered in the estimation of ammonia emission rates. However, livestock manure is currently moved and treated in accordance with the energy recycling policy. In this study, the new emission estimation method were suggested considering the moving and treatment characteristics of livestock manure. A new ammonia emissions was calculated for Bcity and Y-gun at Chungcheongnam-do province. It was shown that the moving and treatment ratio of ammonia emissions were 4.3% and 8.4%, respectively. Furthermore, this ratio would be increased rapidly with the continuous establishment of large-scale manure treatment facilities.
        4,000원
        2849.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        During the aerobic composting of livestock excrement, or leachate, which is generated in the composting process, is distributed through a bulking agent. In this research, a specific microorganism expected to reduce the emission of odor was activated in livestock excrement supernatant, which is similar to the leachate used for water control. While the microorganism was being diffused, odor properties were examined. The bulking agent, in which the specific microorganism was activated, was produced by applying a bioreactor filled with pumice and pellets to the treatment process of livestock excrement activation sludge method. The dominant microorganisms were Pseudomonas genera. Experiment results showed ammonia content was lowered as to 0.27 ppm 1n 15 days; however, it remained at levels of 3.15 ppm in the control reactor. After the composting time was completed, the ammonia concentrations were 0.07 ppm and 3.43 ppm, respectively. The complex odor in the test reactor was 28~30 times greater than of dilution, but was 42~45 times greater in the control reactor. Hydrogen sulfide and methyl mercaptan were not detected in either reactors. Ammonia was thought to be the major odor-producing source during the aerobic composting process of livestock excrement, and it was expected that the odor could be reduced by spraying a microorganism -activated solution as a bulking agent.
        4,000원
        2850.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In this study, a correlation analysis of odor was performed in order to assess the reliability and the field applicability of the Odorous gas sensor for continuous real-time monitoring. Hydrogen sulfide was found to have a correlation of 41.5~65.8%, and Ammonia is was found to have very low correlation in less than 200 ppb concentration. Reactivity evaluation result, hydrogen sulfide is the reactivity was higher than the low concentration condition of 100 ppb or less indicated by 31.3~36.4% in the 100 ppb or more high density condition based on the reference density value. For ammonia was very low reactivity in the low-concentration conditions below 200 ppb. TVOC and composite odor assessment did not occur Reactivity no reference concentration value, the specific comparison between both sensors showed a similar trend. In the same Odorous gas sensor accuracy between the result, 40.3~130.6% hydrogen sulfide, ammonia, 69.1~104.9%, TVOCs is 24.7~98.6%, exhibited human odor intensity from 5.5~33.2%.
        4,000원
        2851.
        2015.09 KCI 등재 구독 인증기관·개인회원 무료
        The transferring guaranteein Romebut because ofinherent defectsreplaced by theof pledge,and otherway. The transferring guaranteetheof modernright for securitybecause of itsong social functiongradually revealed, beganthe judicial practice of RenaissanceIn particular,widely usedGermany as the representative of theand regions, theresearch and practical applicationguaranteeJapantended to improve. In our country, In thelaw"consideration of the draft, aroundtransferring guaranteebe"into the law", choose which way "into the law", the academic circlescivil law in our countryunched a fierce debate, so far no results. But, the transferring guaranteeproblemsreality of our countrythe practice,need toby one. Transferring guarantee is a kind of new real rights for security different from mortgage and pledge,by the perspective of China under each make and guarantee problems arise in practice, In the perspective of the framework regulation puts forward the solutions. At the same time, the author let doctrinal issues guarantees for two cases of collateral typical case discussion and analysis.Finally, after transferring guarantee feasibility in China, The author claimed on composition of the security right, given the make and guarantee the subject of extensive and atypical way, China should take into account the theoretical logic and practical usefulness of the civil special law to regulate the mode of collateral, fundamental solution to the problem of the existence of the guarantee in practice.
        2852.
        2015.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The purpose of this study was to investigate the odor arising from the Cheong-ju industrial complex area for odor materials confirmation, and to predict the impact of the odor in the residential area using the CALPUFF Model. Among the odor causing substances in the area with a rising number of collective complaints due to odor, methyl sulfide, acetaldehyde, propionaldehyde, n-buthylaldehyde, n-valeraldehyde and styrene were detected. Odor causing substances detected in the area surrounding the industrial complex include ammonia, hydrogen sulfide, n-buthylaldehyde, toluene, xylene, benzene and styrene. Using the CALPUFF Model, it was predicted that 1hr average was 3.981~7.553 OU/m3 and 24hr average was 1.753~2.359 OU/m3. In terms of odor intensity, the predicted 1hr average was 0.6~0.9 and the 24hr average was 0.2~0.4.
        4,000원
        2853.
        2015.09 구독 인증기관 무료, 개인회원 유료
        A 5-year-old, 8.95 kg, female Schnauzer presented anorexia with a 3-day history and increased heart sound intensity. Based on the clinical and echocardiographic findings along with the positive blood culture result, the dog was diagnosed with infective endocarditis (IE). Using proper antibiotics treatment, clinical signs were improved within 3 days and resolved within 1 week. For exact identification of the causative agent, multiplex polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) methods were performed. The etiological agent was confirmed as Staphylococcus pseudintermedius with antibiotics resistance genes such as β-lactamase (blaZ) and methicilline resistance (mecA). The bacterial virulence factors included pyogenic toxin genes such as staphylococcal enterotoxins A, B, C, D, and E and toxic shock syndrome toxin 1. Diagnosis of IE is challenging due to a variety of non-specific clinical presentations, rapid disease progression, and lack of a confirmative diagnostic technique. This report demonstrated that such molecular diagnostics could be very useful for diagnosing and identifying characteristics of the causative organism for prediction of prognosis and proper treatment. To our knowledge, this is the first report on the isolation of S. pseudintermedius using molecular diagnostics from a clinical case of canine IE.
        4,000원
        2854.
        2015.09 KCI 등재 구독 인증기관·개인회원 무료
        회사 내외에서 막강한 권한을 행사하는 발기인, 이사, 집행임원, 감사, 감사위원, 지배인, 검사인, 청산인, 사채권자집회의 대표자 등이 청렴하여 야 국민경제가 발전하고 깨끗한 사회, 정의로운 사회를 구현될 수 있을 것이다. 따라서 상법은 이들에게 무거운 법적 책임을 지우고 이를 위반 할 경우에는 민사책임을 부담시키는 외에도 행정목적의 실효성 제고를 위하여 엄중한 형사 처분을 인정하고 있다. 그러나 회사에 있어서 이사 등 임원의 혼탁한 양상은 너무 많은데도 상법 제630조가 적용되어 독직 죄가 인정된 사례를 찾아보기 힘들다. 이 연구는 그 동안 사문화되다시피 하여 학계와 실무계의 관심 밖에 있던 동 법제를 정비할 수 있는 단초를 제공함으로써 이와 관련된 범죄 가 발생할 경우, 동조의 적용을 가능하게 하고, 그러한 범죄를 예방할 수 있도록 하여 깨끗하고 투명한 사회, 정의로운 경제 질서 확립에 기여하 는 한편 이사 등 임원의 직무집행의 공정성과 이에 대한 사회의 신뢰를 보호하는데 보탬이 되고자 한다. 이 연구는 독직죄에 관한 이론구성과 그 본질을 함께 하는 형법상의 뇌 물죄를 비교하면서 법제를 정비하기 위하여 필요한 부분을 보충하는 형식 을 취하고 있다. 그 결과 법제를 정비하기 위해서는 행위주체에 사실상의 이사(상법 제401조의2 업무집행지시자 등)를 포함해야 한다는 점, 재산상 이익의 개념 확대, 처벌 강화, 그리고 본조의 적용을 위한 해석기준으로서 직무범위를 구체화해야 할 필요성 및 윤리규준의 마련 및 지속적인 교육 홍보활동 추진, 지속적인 감시감독활동의 강화, 고소고발의 활성화와 고발 인 보호제도, 독립된 기업수사기관 설치 등을 제안하고 있다.
        2855.
        2015.09 구독 인증기관 무료, 개인회원 유료
        Traditionally, Centella asiatica leaf extracts are used to treat neurodegenerative diseases in India. Centella asiatica is reportedly used to enhance memory and treat dementia, but its promoting effect on neural stem cell differentiation has not been studied yet. In the present study, we investigated whether or not Centella asiatica leaf extracts act on neuronal precursor cells and neuronal cell lines to induce neuronal differentiation, neurite outgrowth, and neuroprotection. The neurogenesis-promoting potential of Centella asiatica leaf extracts was determined by differentiation assay on neural stem cells isolated from mouse embryos and PC12 cell lines. To understand the contribution of specific neural cell types towards increase after Centella asiatica treatment, neural stem cells were differentiated into various neural subtypes and checked by Western blotting using neural cell lineage-specific antibody markers. Neuroprotective activity of Centella asiatica was analyzed in PC12 cells exposed to 100 μM of H2O2. Cell growth was analyzed by MTT assay while cell death was analyzed by Western blotting detection of apoptosis-related proteins. Cells treated with Centella asiatica had significantly longer primary and secondary neurites as well as a higher number of neurites per cell compared to control cells. Expression levels of TUBBIII, TH, NF, and BDNF increased upon Centella asiatica treatment, suggesting that Centella asiatica has a neurogenesis-promoting effect. Centella asiatica also inhibited oxidative stress-induced neural cell damage through regulation of apoptosis- and cell cycle-related proteins. Thus, leaf extracts of Centella asiatica might promote neurogenesis, neuroregeneration, and neuroprotection in the context of neurodegenerative diseases.
        4,000원
        2856.
        2015.09 구독 인증기관 무료, 개인회원 유료
        The intradermal test (IDT) has been developed for confirming diagnosis of canine atopic dermatitis (CAD). Prior to performing IDT, rapid immunoassay (Allercept E-screen 2nd generation; ES2G) can detect allergen-specific immunoglobulin E (IgE) antibodies in canine serum. The objective of this study was to evaluate agreement between IDT and immunoassay in diagnosis of CAD in domestic atopic dogs. Forty dogs were diagnosed with CAD in accordance with Favrot’s criteria. Intradermal testing was performed using 39 selected allergens. ES2G detected IgE antibodies specific for three allergen groups, including indoor allergens, grasses and weeds, and trees. Among 19 dogs diagnosed by IDT, the highest positivity was observed in house dust mites, followed by molds, epidermis and inhalants, house dust, and weeds. A total of 28 atopic dogs were evaluated by rapid ES2G immunoassay. Indoor allergens showed the strongest positive reaction, followed by grasses/weeds and trees. IDT and ES2G were performed concurrently in 17 dogs. The results of ES2G showed slight agreement with those of IDT. Level of agreement was highest for indoor allergens, which showed a predictive positive value of 100% in ES2G. These results indicate that a rapid immunoassay may be valuable for predicting the results of IDT in atopic dogs sensitized to indoor allergens.
        4,000원
        2857.
        2015.09 구독 인증기관 무료, 개인회원 유료
        우리나라는 대기업, 재벌에 대한 반기업정서가 상대적으로 크게 형성되어 있어 반기업정서에 대한 논의가 중요하게 다뤄지고 있다. 이러한 배경 하에서 본 연구는 반기업정서와 기업 평판, 사회공헌 활동의 관계 및 영향력에 대해서 평상시와 위기시로 구분하여 분석하여 보았다. 연구결과는 다음과 같다. 첫째, 본 연구는 실제 우리나라 유수 대기업들의 반기업정서 태도에 대하여 기업별 점수를 측정하여 삼성, SK, 현대, CJ가 반기업정서가 높은 기업임을 분석하였다. 둘째, 반기업정서들은(인지적 차원이건, 감정적 차원이건) 모두 조직평판에 부정적인 영향을 미치는 것으로 나타났다. 셋째, 위기 상황, 그것도 기업의 도덕적 위기 상황에서는 기업의 사회적 책임 활동이 반기업정서가 평판에 영향을 미치는 데 있어서 더욱 높은 영향력 변인으로 작용하는 것으로 나타났다. 이러한 결과는 기업이 평상시 지속적인 사회적 책임 수행의 필요성에 대한 함의를 던진다.
        8,600원
        2858.
        2015.09 구독 인증기관 무료, 개인회원 유료
        Norovirus (NoV) is an etiologic agent of human and animal acute gastroenteritis and is a member of the family Caliciviridae. NoV is classified based on nucleotide sequences of the VP1 gene into at least six genogroups (GI-GVI), among which GI, GII, and GIV are known to infect humans and GII is the most prevalent genogroup. In this study, VP1, the full gene of GII human NoV, was cloned from a human fecal sample and expressed using a baculovirus expression system. Human NoV VP1-specific monoclonal antibodies (MAbs) were produced using expressed recombinant VP1. Expressed VP1 in the recombinant virus was confirmed by polymerase chain reaction (PCR), indirect fluorescence antibody (IFA) test, and Western blot analysis. Eight hybridomas secreting VP1-specific MAbs against human GII NoV were generated and characterized. All of the MAbs produced in this study reacted with human GII NoV VP1-recombinant baculoviruses but not with other non-human calicivirus recombinant baculoviruses. These MAbs reacted specifically with human NoV GII.4-2009 virus-like particles (VLPs), and some MAbs showed cross-reactivity with other GII.4 variant VLPs. Expressed human GII NoV VP1-recombinant protein and MAbs specific to this protein can be used as useful reagents for detecting and characterizing human NoV.
        4,000원
        2859.
        2015.09 구독 인증기관 무료, 개인회원 유료
        Mycoplasma hyorhinis (M. hyorhinis) is considered an etiological agent of arthritis in suckling pigs. Recently, some M. hyorhinis strains were shown to produce pneumonia that is indistinguishable from the mycoplasmosis caused by M. hyopneumoniae. In this study, we developed a sensitive and specific PCR assay to detect M. hyorhinis and applied the developed PCR assay for detection of Mycoplasma infection in clinical piglets infected with M. hyorhinis. We developed a new PCR assay using a M. hyorhinis-specific primer pair, Mrhin-F and Mrhin-R, designed from the Mycoplasma 16S-23S rRNA internal transcribed spacer (ITS) region. The primers and probe for the assay were designed from regions in the Mycoplasma 16S-23S rRNA ITS unique to M. hyorhinis. The developed PCR assay was very specific and sensitive for the detection of M. hyorhinis. The assay could detect the equivalent of 1 pg of target template DNA, which indicates that the assay was very sensitive. In addition, M. hyorhinis PCR assay detected only M. hyorhinis and not any other Mycoplasma or bacterial spp. of other genera. The new developed PCR assay effectively detected M. hyorhinis infection in pigs. We suggest that this PCR assay using a M. hyorhinis-specific primer pair, Mrhin-F and Mrhin-R, could be useful and effective for monitoring M. hyorhinis infection in pigs.
        4,000원
        2860.
        2015.09 KCI 등재 구독 인증기관·개인회원 무료
        기존의 사법시험제도의 문제점을 보완하고 새로운 법조인을 양성하기 위하여 2009년부터 전국에 25개의 로스쿨이 설립되었고, 현재 입학 정 원은 2000명으로 운영되고 있다. 이러한 정책변경은 법조인의 숫자를 늘리고, 로펌 간의 경쟁을 통하여 적절한 수임료로 양질의 법률서비스를 받을 수 있도록 하려는 취지에서였다. 2017년까지 사법시험을 폐지하기 로 되어 있고, 그 이후에는 로스쿨을 통해서만 법조인 진입이 가능하게 된다. 그러나 현재 로스쿨 시스템은 많은 비판에 직면해 있다. 그 중 하 나는 로스쿨의 등록금이 지나치게 비싸다는 점이다. 2015년 기준 연간 평균 1,593만원이나 되는 등록금 때문에 로스쿨은 소위 부유층의 전유 물이라는 비난을 받아왔다. 법조인 양성제도를 어떠한 방식으로 운영할 것인가의 문제를 놓고 대 립하는 이해당사자 간의 입장을 조율하는 것은 쉬운 일이 아니다. 만일 로스쿨의 문제점을 부분적인 보완으로 해결할 수 있다면 사법시험을 재 론할 필요성은 없다. 그러나 로스쿨은 치유되기 어려운 본질적인 한계를 지니고 있고, 이 점에서 사법시험의 병존 주장은 로스쿨의 한계를 보완 하기 위한 균형추로서 설득력이 있다. 이 글에서는 법조인 양성제도로서 현재 시행중인 로스쿨제도를 검토하고 이의 문제점을 극복하기 위한 방안을 검토하기로 한다. 논의의 순서는 먼저 법조인 양성제도의 두 축이라 할 수 있는 사법시험과 로스쿨에 대한 여론 을 비교하여 입법과정의 기능이 적절히 작동하고 있는지를 검토하고, 로스 쿨의 한계와 사법시험 병존의 필요성에 대하여 논의한다. 그리고 이를 바탕 으로 로스쿨 운영과 변호사시험의 개선방안을 찾아보고, 최종적으로 법학교 육의 정상화를 위한 사법시험의 개선방안을 다루기로 한다.