Oyster mushroom is one of the most widely cultivated and consumed mushrooms in Korea, and mechanization and automation of cultivation systems have enabled mass production. Many cultivars have been developed to replace the old ones such as ‘Suhan‘ and ‘Chunchuneutari 2 ho,‘ which have been cultivated for over 20 years. Among these, ‘Soltari‘ was developed in 2015. Although it has excellent quality, its cultivation is challenging and the productivity is somewhat lower. To address these issues, the Mushroom Division at the National Institute of Horticultural and Herbal Science selected the genetic resource KMCC05165 and attempted hybridization between monokaryons from KMCC05165 and ‘Soltari(KMCC04940)’. Through repeated cultivation tests and evaluation of fruiting body characteristics, the superior strain ‘Po-2019-smj22’ was selected and finally named ‘Otari‘. The optimal mycelial growth temperature of ‘Otari’ was between 25 and 30°C and optimal fruiting body growth temperature was between 13 and 18°C. Mycelial growth on PDA medium was best at 25°C, and at the same temperature, mycelial growth was similar across four media: PDA, MEA, MCM, and YM. In 1,100 mL bottle cultivation, the yield was approximately 174 g, which is about 5% higher than the control cultivar ‘Soltari‘, and the number of valid individuals was also higher at about 25. The diameter and height of the pileus were 29.8 mm and 17.6 mm, respectively, slightly smaller than ‘Soltari‘, and the stipe was thin and long with a thickness of 12.2 mm. Additionally, the pileus’ lightness index (L index) was 30.7, indicating a darker brown color compared to 'Soltari.' With excellent mycelial growth, ease of cultivation, and high yield, the new cultivar ‘Otari‘ is expected to be widely adopted by domestic oyster mushroom farms.

Recently, active research in Korea and worldwide has begun to focus on gene function and cultivar development using gene editing tools. This research, in addition to studies on edible mushroom, aims to enhance the physical and biochemical characteristics of mushrooms for applications in materials and substance production. For these studies, efficient isolation of protoplasts from the target mushroom is critical. However, several commercial cell wall-lysing enzyme cocktails, including Novozyme234, Glucanex, and Lysing enzymes, have recently been discontinued. In this study, we aimed to identify alternative enzyme systems to replace the discontinued cell wall-lysing enzymes for stable isolation of protoplasts from Ganoderma lucidum. To select an optimal osmotic buffer, enzyme function in 0.6 and 1.2 M Sorbitol, Sucrose, Mannitol, and KCl was assessed. The effect of reaction time was also evaluated. Protoplast isolation efficiency of each alternative enzyme was tested using lysing enzymes from Trichoderma harzianum, Chimax-N, and Yatalase, either individually or in combination. This matrix of studies identified enzymes and optimal conditions that could replace the discontinued lysing enzymes.

본 연구는 헬스케어 식물 생산단지의 친환경 설계 및 운영을 위해 우선적으로 고려해야 할 주요영역과 세부요소를 도출하고자 하였다. 헬스케어 식물 생산단지는 환경 친화적 식물 생산시설과 이용자의 정 신 및 신체적 건강 도모 시설을 포함한 복합 엔터테인먼트형이자 지속 가능한 식물 상업시설로 정의된 다. 이를 위해 AHP기법을 활용하여 전문가들을 대상으로 다섯 가지 주요영역(토지, 물, 서식지, 탄소/에 너지/대기환경, 재료/폐기물)과 세부요소에 대한 중요도를 평가하였다. 또한 농장 운영자를 대상으로 친환경 시설의 중요성을 조사하여 시설형과 농장형 유형에 적합한 우선순위를 도출하였다. 연구결과 물 관리가 가장 중요한 요소로 평가되었으며, 토지 관리와 탄소/에너지/대기환경 관리도 높은 중요성을 보였다. 반면 서식지 관리와 재료/폐기물 관리는 상대적으로 낮은 평가를 받았다. 또한 시설형, 농장형, 혼합형으로 유형화된 식물 생산단지 유형별 분석에서는 중요도 평가결과가 다르게 나타났으며, 전문 가와 운영자 간의 평가 차이를 통해 이론과 실무자적 관점의 차이를 확인할 수 있었다. 이러한 연구결과는 헬스케어 식물 생산단지의 맞춤형 친환경 설계 전략수립에 기초자료로 활용될 수 있다.

We presented foundational findings regarding the occurrence and acoustic characteristics of the finless porpoise through passive acoustic and visual surveys conducted on the southern coast of Korea, specifically at Hadong Jungpyeong Port. Over a survey period spanning from July 8 to August 16, 2023, totaling 40 days, we observed peaks in the number of clicks produced by this species on July 15, July 24, August 4, August 11, and August 16. The highest count, totaling 18,924 clicks, was recorded on July 15th, while the lowest count, at 3,888 clicks, occurred on August 8th. Examining the acoustic characteristics throughout the diurnal cycle, we found that the peak activity in terms of DPM (detection positive minute for one hour), DP10M (DPM for ten minutes), and overall number of click sounds was observed between 05:00 and 08:00, with a secondary peak occurring from 17:00 to 18:00. The quietest period was noted between 23:00 and 02:00. Furthermore, there was a significant increase in the number of clicks from sunrise, with the maximum count of 21,581 clicks recorded at 6 AM. This count gradually decreased until noon, experienced a slight increase thereafter, peaked again at sunset, and then declined. The dominant frequency mode of this species was 126 kHz, with a concentration ranging from 112 to 136 kHz. The average duration of a click sound was 127 ㎲, with approximately 16 sinusoids (cycles) within each click sound and an average cycle length of approximately 7.9 ㎲. These findings from our study are anticipated to serve as foundational data for the development of a Korean pinger and acoustic warning system.

This study explored effects of a sludge-based biochar addition on nitrogen removal of membrane bioreactor (MBR) for wastewater treatment. The membrane fouling reduction by the biochar addition was also investigated. A dose of 3 g/L of the biochar was applied to an MBR (i.e., BC-MBR) and treatment efficiencies of organic matter and nutrient were analyzed. The MBRs with powdered activated carbon (i.e., AC-MBR) and without any additives were also operated in parallel. The average removals of COD and TN were improved with the biochar addition compared to those with the control MBR. Interestingly, operational duration was also increased with biochar addition. The CLSM analysis revealed that biomass amounts of BC-MBR and AC-MBR were reduced by more than 40%, and thickness of the biofilm attached to the membrane surface also was decreased. The physical properties of biochar surfaces were compared with a commercial powdered activated carbon. The specific surface area with 38 m2/g and pore volume with 0.13 cm3/g of the biochar were much smaller than those of the powdered activated carbon, which were 1100 m2/g and 0.67 cm3/g, respectively. Manufacturing conditions for the biochar production needs to be further investigated for enhancing physical properties for adsorption and biological improvement.

철근은 콘크리트와 결합하여 인장 능력을 보완하고 표면 피막으로 부식을 방지하지만, 탄산화 및 염화물 침투로 인해 부식이 발생하면 내력이 저하된다. 이를 해결하기 위해 내부식성이 뛰어난 GFRP(Glass Fiber Reinforced Polymer) 보강근이 철근 대채제로 주 목받고 있다. 본 연구는 직경에 따른 GFRP 보강근의 부착특성을 철근과 실험적으로 비교 분석하였다. 콘크리트는 약 39MPa의 고강도 에 가까운 콘크리트를 사용하였으며, 보강근의 직경은 D10과 D13을 사용하였다. 실험 결과, GFRP 보강근의 평균 부착 응력은 17.21MPa로 철근의 18.14MPa와 유사하게 나타났다. GFRP 보강근의 슬립 양은 3.05mm로 철근의 1.53mm 보다 크게 나타났다. 또한 GFRP의 경우 인발 과정에서 표면에 국부손상이 발생하는 것을 확인하였다.

이 연구는 금융교육 표준안 활용 경험이 있는 초등교사의 금융교육 표준안 활용의 어려움을 기술 및 분석한 후 금융교육 표준안 개정에 대한 시사점을 제언하고자 수행되었다. 연구를 수행하기 위해 A 연구회의 금융교육 교육과정 개발에 참여한 초등교사 14명을 연구 참여자 로 선정하였고, 심층면담을 통해 자료를 수집한 후 질적 분석을 수행하였다. 분석을 수행한 결과 교사들이 경험한 금융교육 표준안 사용의 어려움을 네 가지 범주로 분류하였고 이는 다음과 같다. 첫째, 교사들은 초등학생의 수준이 고려되지 않은 금융교육 표준안의 단일한 성취기준에 의해 어려움을 겪었다. 둘째, 교사들은 성취기준 진술 방식으로 인해 자율성이 제한되는 경험을 했다. 셋째, 교사들은 금융교육 표준안의 일부 교수학습 방법 및 유의사항 에서 실질적인 도움을 받지 못해 어려움을 겪었다. 넷째, 교사들은 수업을 설계하면서 성취 기준 내용의 중첩으로 인해 어려움을 겪었다. 이를 토대로 본 연구에서는 향후 금융교육 표준안 개정에 대한 시사점을 제언하였다.

Recently, domestic fishing production of Japanese horse mackerel has been continuously decreasing. To achieve sustainable fishing of this species, it is essential to acquire its target strength (TS) for accurate biomass estimation and to study its ecological characteristics. To date, there has been no TS research using a broadband echosounder targeting Japanese horse mackerel. In this study, for the first time, we synchronized an underwater camera with a broadband frequency (nominal center frequency of 200 kHz, range: 160-260 kHz) to measure the TS according to the body size (16.8-35.5 cm) and swimming angle of the species. The relationship between Japanese horse mackerel length and body weight showed a general tendency for body weight to increase as length increased. The pattern of the frequency spectra (average values) by body length exhibited a similar trend regardless of body length, with no significant fluctuations in frequency observed. The lowest TS value was observed at 243 kHz while the highest TS values were recorded at 180 and 257.5 kHz. The frequency spectra for the swimming angles appeared to be flat at angles of –5, 0, 30, 60, 75, and 80° while detecting more general trends of frequency spectra for swimming angle proved challenging. The results of this study can serve as fundamental data for Japanese horse mackerel biomass estimation and ecological research.

This study investigated the species composition and community structure of aquatic organisms captured using coastal beam trawling in Gomso Bay, Jeollabuk-do, from January to December 2022. Throughout the experimental period, a total of 20,246 individuals belonging to 94 marine species were captured, with a combined biomass of 602,828 g. Fish exhibited the highest abundance, comprising 56 species, followed by crustacea (21 species), bivalvia (8 species), cephalopoda (5 species), gastropoda (3 species), and holothuroidea (1 species). The dominant species was Leiognathus nuchalis, constituting 14.0% of the total individuals, followed by Portunus trituberculatus at 12.1%, Oratosquilla oratoria at 10.4%, Crangon hakodatei at 9.9%, and Metapenaeus joyneri at 7.9%. The diversity index ranged from 1.72 to 2.55, with the lowest diversity observed in March and the highest in July. Cluster analysis based on species composition of the 27 most common species showed that aquatic organisms were divided into three groups: spring and summer organisms (Group A) and summer organisms (Group B) and autumn and winter organisms (Group C).