By using the Optical Wide-field Patrol (OWL) network developed by the Korea Astronomy and Space Science Institute (KASI) we generated the right ascension and declination angle data from optical observation of Low Earth Orbit (LEO) satellites. We performed an analysis to verify the optimum number of observations needed per arc for successful estimation of orbit. The currently functioning OWL observatories are located in Daejeon (South Korea), Songino (Mongolia), and Oukaïmeden (Morocco). The Daejeon Observatory is functioning as a test bed. In this study, the observed targets were Gravity Probe B, COSMOS 1455, COSMOS 1726, COSMOS 2428, SEASAT 1, ATV-5, and CryoSat-2 (all in LEO). These satellites were observed from the test bed and the Songino Observatory of the OWL network during 21 nights in 2014 and 2015. After we estimated the orbit from systematically selected sets of observation points (20, 50, 100, and 150) for each pass, we compared the difference between the orbit estimates for each case, and the Two Line Element set (TLE) from the Joint Space Operation Center (JSpOC). Then, we determined the average of the difference and selected the optimal observation points by comparing the average values.

Much effort has been expended to find agronomically important QTLs for improving soybean yield. However, the complexity of genome, such as genome duplication, limits the utility of genome-wide association studies and linkage analyses to identify genes controlling yield traits. We propose the variation block method, a three-step process for recombination block detection and comparison. The first step is to detect variations by comparing short-read DNA sequences of the cultivar to a reference genome of the target crop. Next, sequence blocks with variation patterns are examined and defined. The boundaries between the variation-containing sequence blocks are regarded as recombination sites. All the assumed recombination sites in the cultivar set are used to split the genomes, and the resulting sequence regions are named as variation blocks. The practicality of this approach was demonstrated by the identification of a putative locus determining soybean hilum color and known genes such as flower color gene. We suggest that the variation block method is an efficient genomics method for recombination block-level comparison of crop genomes. We expect that this method holds the prospect of developing crop genomics by bringing genomics technology to the field of crop breeding.

The control of flowering, transition from vegetative to reproductive stage, is crucial for significant success during plant development. Multiple environmental and developmental signals are transmitted to the shoot apical meristem and converted to local cue to process developmental phage. These crucial process are delicately controlled and regulated by expression of tissue specifically expressed genes involved in inflorescence development. Therefore, it is necessary that molecular mechanism associated with inflorescence development is revealed to understand control of flowering by genome-wide expression pattern of inflorescence specific genes. In this study we used Affymetrix GeneChip Wheat Genome Array for genome-wide analysis of the expressed genes of inflorescence development including apical meristem and developing spikelet to understand the mechanism of floral development in early stage of wheat inflorescence. Moreover, meta-analysis of 1479 microarray dataset of GPL 3802 provided by Gene Expression Omnibus (GEO) was conducted to determine expression pattern of each probe throughout whole life cycle. Based on meta-analysis, we demonstrate inflorescence specific expressed genes in wheat inflorescence including apical meristem, spikelet meristem to understand the mechanism of floral development of wheat inflorescence.

Seed color is an important factor affecting physiological and developmental process in wheat. One of the plant pigments, anthocyanins are a group of flavonoid compounds well known as pigments responsible for blue, purple, red, or yellow coloration of plant tissues. In this study, we investigated the pigmentation of purple and yellow color seed according to wheat grain developmental stages. The contents of anthocyanin and chlorophyll in the purple and yellow seeds were measured. Chlorophyll contents were changed similarly in both purple and yellow color seed, and no significant difference was observed between them. In purple color seed, the content of anthocyanin was significantly induced compared with yellow color seed. The individual anthocyanin components were investigated by ultra performance liquid chromatography (UPLC). Cyanidine-3-glucoside (C3G) and peonidine-3-glucoside (P3G) were detected as predominant anthocyanin in purple color wheat. To investigate whether structural genes in anthocyanin biosynthesis were involved in the trait differences between purple and yellow color seed, we examined the expression of anthocyanin biosynthesis-related genes (CHS, CHI, F3H, DFR, ANS, UFGT) and MYB transcription factor in developing wheat grains by using qRT-PCR. This study indicates that the expression of anthocyanin biosynthesis-related genes and MYB transcription factors correlate with anthocyanin levels of grain.

High yield is the most important trait in various agricultural characteristics. Many approaches to improve yield have been tried in conventional agricultural practice and recently biotechnological tools employed for same goal. Genetic transformation of key genes to increase yield is one way to overcome current limitation in the field. We are producing transgenic soybean plants through high efficient transformation method by introducing all gene member with AT-hook binding domain, hoping to obtain manageable delay of senescence. Many transgenic soybean plants are growing in greenhouse and GMO field, and will be evaluated their senescence and any association with yield increase.

Soybean is a crop of importance economically and nutritionally in many parts of the world. Thanks to many new genes brought from genomic research, It is possible to introduce various candidate genes through genetic transformation to see the performance of the genes in field. In our lab, soybean transformations have been tried for last 10 years to probe the possibility of traits improvement by transformation of new gene into soybean. For this purpose, three different genes were transformed into Korean soybean variety, Kwangan. First, the gene that controls early flowering of plant was transformed into Kwangan. Second, a candidate gene for soybean mosaic virus (SMV) resistance was transformed to produce transgenic plants. Third, another candidate gene for drought tolerance was transformed. All the transgenic plants from three genes transformation were produced for their gene insertion and their expression using PCR, qRT-PCR. Further analysis including harvesting seeds is currently undertaken.

Copper (Cu) is an essential micronutrient required for growth and development of plants. But, at a high concentration in soil, copper acts as a major toxic element to plant cells due to its potential inhibitory effects against many physiological and biochemical processes. In this study, the morphological and physiological changes were observed in the leaf of sorghum plants treated with different concentrations (0, 100, and 150 μM) of Copper (Cu). The results linked to morphological changes that plants treated with Cu suffered reduction in growth and morphological changes. In the ion concentration investigation, the concentrations of Cu2+ increased, the concentration of others interacting ions (Zn2+, Ca2+, Mn2+, Fe2+) were changed dramatically. For proteome analysis, 2-D combined with MALDI-TOF-TOF mass spectrometry was performed. Two dimensional gels stained with silver staining, a total of 422 differential expressed proteins (≥ 2-fold) were identified using Progenesis SameSpot software. A total of 24 spots from Cu-induced sorghum leaf and 21 spots from Cu-induced sorghum root were analyzed by mass spectrometry. Out of 24 protein spots from Cu-stressed leaf, of which 16 protein spots were up-regulated and 8 protein spots were down-regulated whereas out of 21 protein spots, a total of 9 protein spots were up-regulated and 12 spots were down-regulated from Cu-stressed root. Taken together, these studies revealed the effects of heavy metal, Cu on the growth and physiological characteristics in sorghum seedlings and proteome investigation, hoping to provide references on the mechanism of heavy metal damaging plants.

Space has many distinguishable characteristics from earth such as strong cosmic radiation, microgravity, supervaccum and weak magnetic field. For this reason, space environments can be used an efficient mutagen for plant breeding nowadays. To identify the affected genes by condition in space with outer space, Brachypodium seeds were placed in the Russia Segment (RS) Biorisk module of International Space Station (ISS). Brachypodium distachyon is a model system for temperature grass, because they represent the characteristics for annual winter grass. Seeds and organs of plants carried by satellite or spacecraft to space can be genetically mutated by exposing space environment. We performed a duplicated RNA sequencing to profile the differentially expressed genes. As a results, about 700 genes were upregulated and 250 genes were downregulated by cosmic environments, respectively. In the molecular function category, protein kinase and transcription activity related genes were upregulated. Among the many transcription factors (TFs), stress related TFs such as ERF, NAC and WRKY were differentially expressed in space exposed samples. In the future, their expression will be identified by using qRT_PCR.

The chloroplast (cp) is an organelle with its own genome encoding a number of cp-specific components. The membrane-bound organelles are mainly involved in the photosynthetic conversion of atmospheric CO2 into carbohydrates in which light energy is stored as chemical energy. Resequencing technology via next-generation sequencing has recently been successfully applied which results the field of cp genome characterization is growing fast. Here, we report the complete sequence of the chloroplast genome of Capsicum frutescens, a species of chili pepper. The total length of the genome is 156,817 bp, and the overall GC content is 37.7%. A pair of 51,584-bp inverted repeats (IRs) is separated by a small (17,853 bp) and a large (87,380 bp) single-copy region. The C. frutescens chloroplast genome encodes 103 unique genes, including 79 protein-coding genes, 20 tRNA genes, and four rRNA genes. Of these, 19 genes are duplicated in the IRs and 18 genes contain one or two introns. Comparative analysis with reference cp genome revealed 125 simple sequence repeat (SSR) motif and 34 variants, mostly located in the non-coding regions. These microsatellite markers will facilitate the studies of genetic diversity, population genetic structure, and sustainable conservation for C. frutescens.

Anthocyanin, a group of purple or reddish flavonoids, have been recognized as health-promoting functional food ingredients due to antioxidant activity. For this reason, plant breeders are trying to increase the anthocyanin contents using methods such as classical breeding and biotechnological approaches. To broaden the mutants population, seeds of colored wheat variety (K4191) were irradiated by using 250 Gy gamma irradiation. Individual 968 M4 plants were grown in Korea Atomic Energy Research Institute field. Many mutant phenotypes were shown: seed color variation, abnormal spike shape, awning formation, heading and ripening time, plant height, ripening period, super dwarf, etc. To identify the inheritance traits of colored-wheat, individual lines were maintained the spike base classified by generation. Characteristics per spike and plant were piled up to construct for mutant database. In the future, fixed descent will be analyzed the anthocyanin contents or other phytonutrients by ultra-performance liquid chromatography (UPLC). Expression of seed color-related transcription factors and anthocyanin biosynthetic pathway genes will be examined.

This study was performed to investigate the growth characteristics and inorganic components of Codonopsis lanceolata regarding regional differences. The plant height of Japanese Codonopsis lanceolata was 373.6 cm, so it’s revealed that it has more vigorous growth than Korean won. The flowering time of Korean Codonopsis lanceolata was 2 weeks faster than Japanese one. Total fresh weight of root was 41.0 g and 39.0 g for Korean and Japanese respectively, thus, no significance difference was found. However, regarding fresh weight, Korean one had a more fresh weight (35.4 g) of main root parts, but Japanese one had a more fresh weight (9.6 g) of the lateral root part. Each inorganic component was found more in the aboveground parts, regardless of the region and the content of K was the largest. Regarding the content of macroelements for each part of Codonopsis lanceolata, the content of Na, Mg, P, S, and Ca in Korean Codonopsis lanceolata was found the highest on the leaf, followed by stem and root. In the case of Japanese Codonopsis lanceolata, same result was found on the content of Mg and Ca, however, the highest content of Na and P was found in the stem.

This study was conducted to compare the growth, inorganic components, and proximate components of Codonopsis lanceolata grown in 10 regions of Korea for selecting superior species and breeding by crossing. Among the all tested lines, the shortest plant height (217.12 cm) was observed from the Ulleungdo region line (No. 4) while the longest (273.9 cm) was observed from Hwasun region line (No. 9). In addition, the lines of central and northern region (No. 1~No. 7) tend to have shorter plant height than those of southern region (No. 8~No. 9) except Jejudo region line (No. 10). Flowering tends to be late towards southern region, and lines in central and northern regions were started flowering about 2 weeks earlier than those in southern regions. However, the heaviest root weight was 13.1 g, found in only Jejudo line (No. 10) whereas there was no significant difference found in the other regions which have a range of 8.3~11.0 g. The inorganic components were varied in each line, however, proportion of macroelements, such as K, Ca, and P, was the largest for every line. Especially for Heongseong region line (No. 2), had larger proportion of macroelements than the others. There was a difference of proximate compositions of Codonopsis lanceolata, except the moisture content, among all regions, however, it was generally shown that the content of crude protein (1.31~3.76%) and crude fiber (2.18~3.12%) was the highest.

This study investigated the effect of NH4 + concentrations on microalgae growth by appling mixotrophic microalgae chlorella vulgaris in order to treat anaerobic digested food waste leachate. The growth rate and final microalgae growth were an order as 400 > 100 > 800 > 1300 mg-N/L. As results, The growth rate and final growth of microalgae were highest at ammonia concentration of 400 mg-N/L, On the other hand microalgae growth was inhibited when ammonia concentrations were over 800 mg-N/L. high concentrations of nitrogen over 800 mg-N/L interrupt the growth of microalgae. All of nitrogen and phosphorus were removed by microalgae at the ammonia concentration of 100~400 mg-N/L. In addition, when ammonia concentration was over 800 mg-N/L, the removal of nitrogen and phosphorus was limited mainly due to the microalgae growth limit. It was possible to treat anaerobic digested food waste leachate with mixotrophic microalgae when the ammonia concentration was controlled below 400 mg-N/L.

본 연구에서는 수입에 의존하고 있는 고산도 식초 생산 기술을 대체하고자 포도과즙을 이용하여 적정산도 10% 이상의 식초 제조조건 및 품질특성을 조사하였다. 초기 알 코올 함량에 따른 고산도 초산발효에서는 초기 알코올 함량 6%에서 적정산도 12%의 고산도 식초 제조가 가능하였으 며 초기 알코올 함량이 높을수록 유도기가 길어져 초산수율 이 감소하였다. pH는 구간에 따른 큰 차이가 없었고, 당도는 1단계 초산발효(1st AAF)까지 초기 알코올 함량에 비례하 여 높게 나타났으나 2단계 초산발효(2nd AAF)기간 동안에 는 초기 알코올 6% 첨가구에서 증가율이 가장 높았다. 고산 도 포도식초의 주요 유기산은 tartaric, malic 및 citric acid가 검출되었으며, 유가식 첨가량 및 초산 발효수율 차이로 인 하여 초기 알코올 함량이 낮을수록 높게 검출되었다. 미량 알코올 중 ethyl alcohol을 제외한 성분은 극소량으로 검출 되어 초기 알코올 함량에 따른 큰 차이가 없었고, ethyl alcohol은 364∼581 ppm으로 나타났다. 이상의 결과, 포도알코올 발효액(초기 알코올 함량 6%)를 이용하여 일체의 영양원을 첨가하지 않고 총산도 12%의 고산도 식초 제조가 가능하였다. 그러나 산업적으로 활용하기 위해서는 초산수 율 향상 및 유가식 첨가에 의한 발효기간 단축에 관한 보완 연구가 요구되었다.

Osteosarcoma (OS) is one of the most common malignant primary bone tumors and NF-κB appears to play a causative role, but the mechanisms are poorly understood. OS is one of the pleomorphic, highly metastasized and invasive neoplasm which is capable to generate osteoid, osteoclast and osteoblast matrix. Its high incidence has been reported in adolescent and children. Cell signal cascade is the pivotal functional mechanism acquired during the differentiation, proliferation, growth and survival of the cells in neoplasm including OS. The major limitation to the success of chemotherapy in OS is the development of multidrug resistance (MDR). Answers to all such queries might come from the knock-in experiments in which the combined approach of miRNAs with NF-κB pathway is put into use. Abnormal miRNAs can modulate several epigenetical switching as a hallmark of number of diseases via different cell signaling. Studies on miRNAs have opened up the new avenues for both the diagnosis and treatment of cancers including OS. Collectively, through the present study an attempt has been made to establish a new systematic approach for the investigation of microRNAs, biophysiological factors and their target pairs with NF-κB to ameliorate oncogenesis with the “bridge between miRNAs and NF- κB”. The application of NF-κB inhibitors in combination with miRNAs is expected to result in a more efficient killing of the cancer stem cells and a slower or less likely recurrence of cancer.

Gamma irradiation has been used as a tool for plant mutation breeding to select new cultivar with improved characteristics. Generally, the irradiation of seeds with high doses of gamma rays disturbs the synthesis of protein, hormone balance, and enzyme activity. And also, high dose of gamma rays to reduce plant height, number of tiller, and root length, although the effect of gamma-irradiated plants may depend on the species and cultivar or stress conditions. Biological effects of radiations can be divided into two types according to dose range and periods of exposure. Acute irradiation represents exposure to high-dose of irradiation over short period time, whereas the chronic irradiation is comprised of exposure to low doses of radiation over extended period of time. To compare the effects of acute and chronic exposure to ionizing radiation on two wheat cultivars (K4191 and Geumgangmil), we measured their germination rate, seedling height, and root length. In order to understand the influence of antioxidant-related genes and DNA repair-related genes, we used qRT-PCR methods to identify their expression levels. To study the behavior of a radiation-induced free radical, gamma-irradiated seeds were used for ESR spectroscopy. Plant growth pattern was showed positive correlation with ESR results. This study indicates that low level chronic radiation exposure is even more serious effects than short doses of high level radiation according to different wheat cultivar.

Steroidal glycoalkaloids which serve the plant defense, are toxic secondary metabolites present in the plants of solanaceae family. The upper safe limit of glycoalkaloids for human consumption is 20mg/KG FW, excess of which may cause severe health disorders. Several factors like drought, high temperature, light exposure, and wounding increase tuber glycoalkaloid content. Among these, drought is an important factor which causes a rapid increase in potato glycoalkaloid content. Glycoalkaloid biosynthetic genes and their expression pattern need to be characterized to regulate the glycoalkaloid accumulation. Three key genes SGT1, SGT2 and SGT3 are demonstrated to be directly participated in the biosynthetic pathway for glycoalkaloid formation. Present study was focused on the study of expression pattern of key genes in GA pathway under drought stress in two different potato cultivars Atlantic and Haryoung which are low and high glycoalkaloid accumulating respectively. Drought stress was imposed by withholding water to the plants grown in pots and control plants kept irrigated. Expression analysis of SGT1, SGT2 and SGT3 was done from the leaf and tuber sample of three time intervals i.e 5, 10 and 20 days after imposing stress. Variation in the expression level of genes was observed in leaf and tuber where the fold increase in expression over control was higher in tuber sample compared to leaf. Expression levels also varied in leaf and tuber among two cultivars. However, expression of SGT1, SGT2 and SGT3 is significant indicating the involvement of these genes in glycoalkaloid accumulation under drought stress.

Chronic gamma irradiation can be used an alternative mutation breeding methods for induction of many useful mutants. Seedlings of purple-colored wheat plants were irradiated with wide range doses of chronic gamma-rays (20, 25, 30, 40, 50, 70, 100, 125, 150, 200, 250, 300 Gy) during 6 weeks at gamma-phytotron in the Korea Atomic Energy Research Institute, respectively. To identify the biological responses purple-colored wheat, we examined the plant height, chlorophyll, carotenoid and total anthocyanin contents in leaf. Plant growth, chlorophyll and carotenoid contents in leaf were decreased when the dose rate increased. Anthocyanin contents were increased with the increase of the radiation dose until 50 Gy treatment. To confirm the real contents of anthocyanin, we also investigated cyanidin-3-glucoside in purple-colored wheat leaf by using UPLC analysis. These results indicate that anthocyanin accumuation was observed under chronic gamma irradiation.

Acanthopanax species is known commonly as Siberian ginseng, touch-me-not, devil’s shrub, prickly eleutherococc, eleutherococc and wild pepper. A diverse group of chemical compounds isolated from Acanthopanax species was named ‘eleutherosides’. Among eleutherosides, eleutherosides B and E were widely known in Acanthopanax species. Acanthopanax species are cultivated and grow wild in a various area of Korea and have a variety of pharmacological effects. But, there are a lot of difficulties on producing excellent Acanthopanax species, according to the cultivated method is different pharmacological ingredients. This study, therefore, analyzed eleutherosides B and E in A. divaricatus and A. koreanum by different fertilizer ratio using HPLC. We will be investigated a high content of eleutherosides B and E by different fertilizer ratio and suggest an efficient fertilizer ratio of A. divaticatus and A. koreanum. All samples of A. divaricatus and A. koreanum were collected at Yeongcheon Agricultural Technology & Extension Center, Yeongcheon, Korea. The sample was prepared by upper and lower parts. The fertilizer ratio are N-P-K(10.5-8.5-8.5: 50 kg/10a), 2N-P-K (21-8.5-8.5: 50 kg/10a), N-2P-K (10.5-17-8.5: 50 kg/10a), N-P-2K (10.5-8.5-17: 50 kg/10a), and 2N-2P-2K (21-17-17: 50 kg/10a), respectively. To analyze eleutherosides B and E, 5 g of A. divaricatus and A. koreanum was extracted with 50% MeOH (3 × 100 ml) by reflux and evaporated in vacuo. The residue was dissolved in 1 ml of MeOH. The resulting solution was used for HPLC analysis. HPLC separation of eleutherosides B and E for qualitative and quantitative analysis was performed using a reverse phase system. A Discovery®C18 (4.6 × 250 mm, 5 μm) column was used with a mobile phase that consisted of water and acetonitrile. A gradient solvent system of water and acetonitrile (90:10 to 70:30 for 20 min) was used for the elution program. UV detection was conducted at 350 nm. The injection volume was 10 μl and the flow rate was 1 ml/min. All injections were performed in triplicate. The different fertilizer ratio yielded total eleutherosides B and E contents of 4.417-6.905 and 3.652-7.227 mg/g in the upper and lower parts of A. divaricatus, respectively. In A. koreanum, the total eleutherosides B and E contents were 4.591-10.108 and 3.834-9.079 mg/g in the upper and lower parts, respectively. The best conditions to increase eleutherosides B and E content in A. divaricatus was determined to be with N-2P-K fertilizer ratio, on the other hand, in A. koreanum was 2N-2P-2K fertilizer ratio.

The change of quality characteristics with storage temperature (room (25°C), low (15°C) and cold (4°C) temperature) and period of unhulled foxtail millet (Setaria italica Beauv. cv. Samdame and Gyeongkwan 1), proso millet (Panicum miliaceum L. cv. Hwanggeum-gijang and Manhongchal) and sorghum (Sorghum bicolor (L.) Moench cv. Hwanggeumchal and Donganme) were evaluated. The 1,000 grain weight, lightness, redness, yellowness and moisture content with storage temperature and period of unhulled foxtail millet, proso millet and sorghum were not showed difference. Germination percent and milling recovery of foxtail millet, proso millet and sorghum decreased with increasing storage temperature and period. Fat acidity of stored foxtail millet, proso millet and sorghum was increased with increasing storage period, and the higher temperature increased more. The results of this study show that the storage of foxtail millet, proso millet and sorghum at the low and cold temperature is better than the room temperature. Especially, in mind of the economics, the storage method is good low temperature than cold temperature.