This study aims to investigate effects of the length of the buoy and sand bag line on the loss of webfoot octopus pot. A numerical modeling and simulation was carried out to analyze the process that the pot gear affected by wave using the mass spring model. Through the simulation, tensions of sand bag line under various condition were investigated by length of buoy and sand bag line. The drag force and coefficient k of an artificial shell used in the webfoot octopus pot was obtained from an experiment in a circular water channel, and the coefficient k was applied to the simulation. To verify the accuracy of the simulation model, a simple test was conducted into measuring a rope tension of a hanging shell under flow. Then, the test result was compared with the simulation. The lengths of the buoy line in the simulation were 1.12, 1.41, 1.80, 2.23, 2.69, and 3.17 times of water depth. The lengths of sand bag line were 10, 20, 30, and 40 meters, and conditions of water depth were 8, 15, 22 meters. 4 meter height and 8 second period of wave were applied to all simulations. As a results, the tension of the sand bag line was decreased as the buoy and sand bag line were increased. The minimum tension of the sand bag line was appeared in conditions that the length of the buoy line is twice of water depth and the sand bag line length is over 40 meters (except in case of depth 8 meters.).

This study aims to reduce the force exerted to the buoy of the gillnet by wave and current. Five buoy models were selected for experiments and their rope tensions under wave and current action were compared. Five models were EL (ellipsoid), EL-H (ellipsoid-hole), SL (streamlined body), SP (sphere) and CL (cylinder, traditional type). In the first experiment, the Five models were tested without any attachment. In the second experiment, a flagpole was attached to each model. As a result, in the condition without flagpole, the tensions of four models with the exception of the CL were about a half of that of the CL. In the condition with flagpole, the tension of all models was twice larger than that without flagpole. Thus, a new model was suggested to improve the problem, which has a combined body that of a flagpole and a buoy Three new models of CL-L (long and thin cylinder), LF (leaf shape) and LF-F (leaf shape with fin) were designed. Also a cylinder type (CLD) with a flagpole as a control was included in the experiment. As a result, the LF-F had the smallest tension and a half tension of the CLD. Therefore, it is supposed that the flagpole and buoy combined model could reduce the tension on buoy rope and contribute to improve the gillnet loss problem.

The Fishing gear loss has been repeated every year in the West Sea; however, there has been no solution. So fisher men have undergone economic loss every year. Thus it is required to reduce the loss of fishing gear. In this study to find out the reason that the fishing gear is lost in the Sea, 10 years data of wave and current for 6 locations in the West Sea were investigated and a numerical modelling were conducted into the behaviour of a gillnet in wave and current. The fishing gear was modelled with the mass spring model. As a result, it came out into the open that the location where fishing gear loss occurred most frequently was Choongnam province. The height of the maximum significant wave in this province was 6.7 m and the period of that was 4.4 second. The maximum current speed was 0.7 m/s. As a result of simulation with these data, it was revealed that the buoy is one of the reasons to decrease the holding power of the gillnet. For example, the tension of anchor rope was decreased to 50% while the drag coefficient or volume of buoy was decreased to 25%. So it is predicted that an improvement of the buoy contributes to the reduction of the gillnet loss.

단간 내도복성 ‘소담찰’ 수수의 논 재배 시 안정적인 수량확보를 위해 파종시기 및 재식거리에 따른 생육 특성과 종실생산성에 대한 시험을 2014년 경기도 수원의 국립식량과학원시험포장에서 수행한 결과를 요약하면 다음과 같다. 1. 출수기는 6월 10일 파종에서 62일로 가장 빨랐으며, 비닐피복재배에서 파종기가 늦을수록 출수기가 빨라지는 경향이뚜렷하였다. 2. 파종기에 따라서는 6월 20일 파종에서 생육이 급격히 감소하였고 간경, 분지수, 천립중에서 유의적인 차이를 보였고,피복유무에 따른 간장, 간경, 분지수에서도 유의성을 보였다. 3. 주당종실중과 종실수량은 파종기별 비닐피복재배에서는유의적인 차이가 인정되지 않았으나 무피복재배에서는 6월 20일 파종에서 주당종실중이 17.7g, 종실수량이 212 kg/10a으로가장 적었으며, 파종기 간에 유의적인 차이를 보였다. 4. 소담찰 수수는 간장이 평균 90 cm로 매우 작아 논 재배에서 문제가 되는 도복에 강한 경향을 보였으며, 이삭길이는소식재배에서 길어지는 경향을 보였고 간장, 간경, 분지수, 이삭길이, 천립중은 재식밀도 간에 유의성을 보이지 않았다. 5. 주당종실중과 종실수량은 재식밀도 간에 유의한 영향을주지 않았으나 주당종실중은 60×25cm (6,600본/10a)의 소식재배에서 48.0 g으로 가장 많았으며, 10a당 종실수량은 60 × 15 cm(1본)과 60 × 30 cm(2본)의 밀식재배에서 각각 427 kg, 415 kg으로 가장 증수되어 소담찰은 밀식적응성이 높은 경향이었다. 6. 따라서 소담찰 수수는 기계수확 등 생력기술을 적용하여논 대면적 재배를 위해 파종기는 6월 10일, 재식밀도는11,000주/10 a [60 × 15 cm(1본), 60 × 30 cm (2본)가 유리한 것으로 판단된다.

The impacts of climate change on agriculture sector are divided into two factors, namely crisis and opportunity factor. The crisis factor is such as more frequent and more intense disaster. On the other hand, there are also opportunity factors such as extension of crop cultivation period. Godangok and Guseulok were sown at 30 June, 10, 20, 25, 30 July and 5 August in 2011, 2012. It takes about 22 ~ 23 days from silking to harvest when sweet corn hybrids were sown in 30 June, but 25 July-sowing takes 27 ~ 30 days and 8 August-sowing takes 30 ~ 36 days. The accumulated temperature for harvesting of sweet corn was about 1,600 ~ 1,900˚C. The minimum cumulative temperature from seeding to harvest was approximately 520 ~ 590˚C. The growing degree days (GDD) from seeding to harvesting were approximately 213 ~ 353˚C and it was highest in the last sowing. At sowing in 2011 (916 ~ 1,067˚C) and 2012 (911 ~ 1,055˚C) all showed no significant difference to the harvest GDD. Plant height, ear height, stem diameter, and dry matter weight were reduced as planting date was delayed (p < 0.05). Ear weight in Godangok was 715 kg per 10a and in Guseulok was 677 kg per 10a, respectively in two years. The aim of the present study was to investigate the growth and yield of sweet corns during ripening according to the sowing time, and to provide information necessary to improve the quality of sweet corns.

중부지역에서 논 콩 파종기 이동에 따른 생육 및 수량성에 미치는 영향을 구명하고자 콩 조숙종 3품종과 중만생종 3품종을 경기 수원지역에서 5월 21일, 6월12일, 6월29일, 8월1일 4회 파종을 하여2012년부터 2013년까지 2개년 간에 수행하여 주요 생육특성 및 수량성을 비교한 결과는 다음과 같다. 1. 출현율은 파종기 조·만에 따른 일정한 경향이 없었으며, 중만숙종인 태광콩이 가장 높았고 조숙종인 참올, 중만숙종 황금이 가장 낮았으며 연차 간 차이는 없었다. 2. 출현소요일수는 만파될수록 단축되는 경향이었으며 조숙종, 중만숙종 품종에 따른 차이가 있었고 연차 간에 총 평균 출현소요일수의 차이가 있었다. 3. 개화소요일수는 조·중만숙종 품종에 관계없이 만파될수록 개화일수가 단축되었으며 단축폭도 만파될수록 적어졌다. 조숙종이 중만숙종 보다 5.5일이 더 빨랐고, 연차 간에 개화소요일수의 유의적인 차이는 없었다. 4. 주경장은 조파에 비해 만파에서 단축되는 경향이었으며 조숙종이 중만숙종 보다 적었고 연차 간에 주경장의 유의적인 차이는 없었다. 분지수는 조숙종은 일정한 경향이 없었으나 중만생종은 만파될수록 많아지는 경향이었으며, 중만숙종이 조숙종 보다 많았고 연차 간에 차이가 있었다. 5. 주당협수는 조파에서 가장 많았고 중만숙종이 조숙종 보다 많았으며 연차 간 차이는 없었다. 주당립수는 전 파종기조·중만숙종 전체평균치는 중만숙종이 조숙종 보다 더 많았고연차간 비교시 2012년이 2013년보다 더 많았다. 백립중은 조기 3파종기간에는 유의적인 차이가 없었고 만파에서는 현저하게 낮아졌으며, 조숙종이 중만숙종 보다 더 무거웠고 연차 간에 고도의 유의적인 차이가 있었다. 6. 10a당 수량은 6월29일 파종기에서 가장 높았고 그 다음이 6월12일, 5월21일 순으로 낮아졌으며 8월1일 파종기에서 가장 낮았다. 전 파종기에 걸쳐 조숙종과 중만숙종 전체평균치를 보면 중만숙종이 조숙종 보다 많았고 연차 간에 고도의 유의적인 차이가 있었다.

The difference of mooring tension by type of buoy was investigated in the circulating water channel and the wave tank for deducting the most stable buoy from the current and the wave condition. 5 types of buoy made up of short cylinder laid vertically (CL-V), short cylinder laid horizontally (CL-H), capsule (CS), sphere (SP) and long cylinder (CL-L) were used for experiments. A mooring line and a weight were connected with each buoy. A tensile gauge was installed between a mooring line and a weight. All buoy’s mooring tension was measured at the same time for the wave test with periods of 1.5~3.0 sec and wave heights of 0.1~0.3 m, and the current test with flow speeds of 0.2~1.0 m/sec. As a result, the order of tension value in the wave test was CL-H > CL-V > SP > CS > CL-L. In the current test CL-V and CL-H were recorded in the largest tension value, whereas SP has the smallest tension value. So it seems that SP buoy is the most effective in the location affected by fast current. CS is predicted to be suitable for a location that influence of wave is important more than that of current if practical use in the field is considered. And it was found that the difference of mooring tension among buoys in wave is related to the product of the cross sectional area and the drag coefficient for the buoy’s bottom side in high wave height. The factor for the current condition was not found. But it was supposed to be related to complex factors like a dimension and a shape by buoy’s posture to flow.

In this study, two commercial PCR and ELISA test kits were examined for identification of eight animal species (beef, pork, chicken, duck, turkey, goat, lamb, and horse) from raw meat and meat products in Korea. The detection limit in RAW meat ELISA kit® on three types of meat samples blended with beef, pork and chicken, demonstrated that all meat species were differentiable down to 0.2%. RAW meat ELISA kit® on animal species resulted in differentiation rate of 94.5% for beef, 93.3% for pork, 90% for lamb, and 100% for chicken, duck, turkey, goat, and horse. In contrast, Powercheck Animal Species ID PCR kitTM resulted in 100% specificity at 0.05% limit of detection for all meat species. The detection limit of Cooked Meat ELISA kit® on mixed meat samples heat-treated with different temperatures and times, resulted in 0.1% for all heat-treated mixed meat except for chicken at 1.0%. Additionally, ELISA kit on sixty meat products resulted in specificity of 31.8% for ham, 13.6% for sausages, and 12.5% for ground processed products, and relatively low rate for more than 2 types of mixed meats. On the contrary, meat species differentiation using PCR kit showed higher percentage than that using ELISA kit®: 50.0% for ham, 41.7% for sausages, and 28.6% for ground processed meat. Futhermore, PCR kit on 54 dried beef meats detected pork genes in 13 products whereas ELISA kit showed negative results for all products. Hence, the possibility of cross-contamination during manufacturing process was investigated, and it was found that identical tumblers, straining trays, cutters and dryers were used in both beef and pork jerky production line, suggesting the inclusion of pork genes in beef products due to cross-contamination. In this study, PCR and ELISA test kits were found to be excellent methods for meat species differentiation in raw meat and heat-processed mixed meat. However, lower differentiation rate demonstrated in case of meat processed products raised the possibility of inclusion of other species due to cross-contamination during manufacturing process.

신광옥은 다수성 사료용 옥수수 품종 개발을 목표로 2012년에 농촌진흥청 국립식량과학원에서 자식계통 KS172과 KS173을 교잡하여 육성한 다수성 단교잡종이다. 신광옥의 종피색은 황오렌지색이며 입질은 중간종이다. 신광옥은 2008~2009년 동안 생산력검정시험을 거쳐 2010~2012년까지 3지역에서 지역적응시험을 실시하였다. 그 결과 우수성이 인정되어 2012 농작물 직무육성 신품종으로 결정되었고 신광옥으로 명명하였다. 신광옥의 출사일수는 대비품종인 장다옥과 비슷하다. 간장은 장다옥과 비슷하나 착수고율은 장다옥보다 낮고 도복은 장다옥과 비슷한 정도로 강하다. 100주당 이삭은 장다옥보다 17개 더 많고 이삭길이는 장다옥과 비슷하며, 100립중은 장다옥과 비슷하다. 깨씨무늬병 저항성은 중강이며, 그을음무늬병에는 강한 편이다. 검은줄오갈병, 이삭썩음병 및 조명나방 저항성은 중 정도이다. 신광옥의 종실수량은 7.8톤/ha로 장다옥과 비슷하다. 4:1(모본:부본) 재식비율로 동시 파종하여 채종 시험한 결과 종자친(모본) 출사기와 화분친(부본) 화분비산기간이 일치하였으며 채종수량은 3.84톤/ha이었다. 신광옥은 전국적으로 재배가 가능하다.

양안옥은 다수성 사료용 옥수수 품종 개발을 목표로 2012년에 농촌진흥청 국립식량과학원에서 자식계통 KS164과 KS163을 교잡하여 육성한 다수성 단교잡종이다. 양안옥의 종피색은 황오렌지색이며 입질은 중간종이다. 양안옥은2007~2008년 동안 생산력검정시험을 거쳐, 2009~2012년까지 3년간 4지역에서 지역적응시험을 실시하였다. 그 결과우수성이 인정되어 2012 농작물 직무육성 신품종으로 결정되었고 양안옥으로 명명하였다. 양안옥의 출사일수는 대비품종인 광평옥보다 2일 빠르다. 간장은 광평옥과 유의한차이는 없으나 착수고율은 광평옥보다 높고 도복은 광평옥과 비슷한 정도로 강하며 후기녹체성과 이삭비율도 광평옥과 유의한 차이는 없다. 양안옥의 깨씨무늬병은 광평옥과비슷한 중강의 저항성을 보이며, 그을음무늬병도 광평옥과같이 강한 편이다. 검은줄오갈병, 이삭썩음병 및 조명나방도 광평옥과 같이 중 정도의 저항성을 보인다. 양안옥의건물수량은 17.45 톤/ha이며, TDN수량은 11.96 톤/ha로 광평옥과 유의한 차이는 없었다. 양안옥의 종실수량은 8.32 톤/ha로 장다옥과 유의한 차이는 없었다. 4∶1 (모본 : 부본)재식비율로 동시 파종하여 채종 시험한 결과 모본의 출사기와 부본의 화분비산기간이 일치하며 채종수량은 1.79 톤/ha이었다. 양안옥은 전국적으로 재배가 가능하다.

This study aims to analyze the performance of a submersible fish cage which was designed for developing an economical cage system can be applied in korean aquaculture environment easily. To analyze the performance of the designed cage a model test was carried out. In the test, inclination changes of the upper frame and mooring tensions of model cage were measured during the submerging and surfacing motion in still water and wave condition (period: 2s, wave height: 0.1, 0.2, 0.3m). As a result, in the still water condition the model cage kept horizontal balance and inclination degree of the upper frame was about 1˚. In the wave condition, the model cage showed bilateral symmetric up-and-down motion but the average inclination degree of the upper frame was about 0˚. When the model cage reached at a depth of 1m, the up-and-down motion of the cage was decreased by 12% compared with that of at the surface (period 2s, height 0.3m). In the same wave condition, the maximum and average line tension under the bottom position were about 8% and 11% respectively compared with that of at surface.

본 연구는 간척지에서 재배가 가능한 내염성 보리 품종육성을 위한 기초정보를 얻고자 겉보리 두 품종을 대상으로 생육초기 염 스트레스에 따른 생리적 반응과 잎 프로테옴의 발현양상 변화를 분석한 결과는 아래와 같다. 1. 토양의 염 농도가 증가함에 따라 보리의 건물중은 무처리구에 비해 유의적으로 감소하는 경향이었으며, 상록보리는무처리구에 비해 건물중 감소가 작았으며, 선우보리는 컸다. 2. 염처리에 따른 잎의 엽록소 함량을 나타내는 SPAD 값은상록보리가 57.6으로 47.6인 선우보리보다 높았으며, Na+의 함량은 선우보리에서 유의적으로 높았고, K+/Na+의 비율은 상록보리에서 높은 경향을 보였다. 3. 이차원전기영동에 의하여 염 스트레스에 의한 잎 프로테옴의 발현양상을 분석한 결과 47개 단백질 spot이 발현양의차이를 나타냈다. 품종별로 발현양이 증가한 단백질 spot은 상록보리와 선우보리에서 각각 17개와 14개로 나타났고, 발현양이 감소한 단백질 spot은 상록보리와 선우보리에서 각각 28개및 27개로 확인되었다. 4. 염처리에 따른 발현양의 차이를 보이는 18개 단백질을 동정한 결과 ribosomal protein 등 기능과 스트레스와의 관련성이 보고된 10개의 단백질과 ankyrin repeat domain protein등 스트레스 조건에서의 역할이 명확하지 않은 4개의 단백질및 Os02g0753300 등 기능 및 스트레스와의 관련성을 알 수없는 2개의 단백질이 동정되었다.

The aim of this study was to evaluate the TEMPOⓇ STA automated most probable number (MPN) system for the enumeration of Staphylococcus aureus (S. aureus) in comparison with a standard culture method. Artificially inoculated food products with S. aureus - triangle kimbap, sliced spring onion, dried filefish fillet, danpatjuk (sweet red-bean porridge with small rice dumplings)- were tested in this study. Twenty-five grams of each of food samples were added into 225 ㎖ of sterilized phosphate buffered saline in a TEMPOⓇ stomacher bag followed by stomaching for 2 min. One milliliter of the stomached sample was added to a bottle of culture medium. Cards were filled and sealed in the automated filler and then were incubated for 24 h at 37℃. After incubation, the cards were placed in the automated TEMPOⓇ reader and MPN results were generated. For comparison with a culture method, decimal dilutions were prepared from the same homogenized samples described above, transferred onto Baird Parker and Baird Parker-Rabbit Plasma Fibrinogen (BP-RPF) agar plates, and then incubated at 37℃ for 24 h. The performance of TEMPOⓇ STA method is equivalent to the culture method using Baird Parker or BP-RPF agar count plate for the enumeration of S. aureus in foods, eliminating a time-consuming and laborious process.

Real-time PCR could help to provide answers to urgent questions about the incidence, prevalence, and epidemiology of currently emerging food-borne bacteria and diseases as identification and detection tools. The objective of this study was carried out to examine several critical parameters that must be optimized when converting from the ABI Prism 7000 SDS platform to the Cepheid SmartCycler Ⅱ so as to directly use the same primer and probe sequences. A lyophilized master mix-OmniMix HS bead, MgCl2 concentration, and PCR cycling conditions were evaluated so as to convert to a new platform, Smartcycler Ⅱ. The best optimal cycling conditions to detect Cronobacter sakazakii on SmartCycler Ⅱ were as follow: initial denaturation at 95℃ for 2 min followed by 45 cycles of 95℃ for 15 s, and 60℃ for 60 s using OmniMix HS bead contained 6 mM MgCl2 concentration. And the Ct value was 16.97 compared to 23.84 of Ct value in ABI Prism 7000 SDS. This result showed that when the several analytical parameters were taken the consideration for optimization, it could be performed assays between real-time PCR platforms. Also it is need of further study to develop the new single multiplex real-time PCR method for determining various Cronobacter spp. including three subspecies, too.

This study was carried out to evaluate the microbiological characteristics of retail chicken meats stored under various conditions. Nine of whole chickens and nine of chicken breasts were used for bacterial analysis. Each chicken meat was divided into subsamples of 25 g each followed by storage at room temperature (25℃), refrigeration temperature (4 ℃), and freezing temperature (—20℃) for 180 min, 5 days, and 3 days, respectively. The standard plate counts were performed for the enumeration of the total aerobic bacteria. The number of aerobic bacteria was gradually increased by 1 log in samples held at the room temperature for 180 min. There was statistical difference in the number of bacteria between at 0 min and at 180 min of storage. For samples stored at 4℃ for 5 days, the number of bacteria was increased from 5.11 to 7.26 log CFU/g in chicken breast and 3.83 to 6.04 log CFU/g in whole chicken with statistical difference. No significant changes were observed in frozen chicken. The results of this study may provide useful information to consumers for proper storage and safe handling of chicken meats.

Vibrio parahaemolyticus (V. parahaemolyticus) has been recognized as a significant food-borne pathogen around the world. In this study, we investigated the prevalence and antimicrobial resistance of V. parahaemolyticus isolated from raw fishes. A total of 64 samples of raw fishes purchased from a traditional seafood market in Seoul, Korea. were examined for the presence of V. parahaemolyticus using intestines, gills, and fins. Twenty five grams of all samples were enriched in 225ml of alkaline peptone water at 37℃ for 24h and then streaked onto thiosulfate citrate bile sucrose agar. Suspected colonies were inoculated into triple sugar iron agar for biochemical screening test and were finally confirmed with API 20NE strip. Antimicrobial resistance tests were performed with disc diffusion method in accordance with National Committee for Clinical Laboratory Standard. Thirty three V. parahaemolyticus strains were isolated from raw fishes among 33 out of 64 (51.6%). Among 33 isolates, 16 isolates (48.5%) were resistant to ampicillin, 7 isolates (21.2%) were resistant to amikacin, and all isolates were not resist to other antibiotics such as amoxicillin & clavulanic acid, sulfamethoxazole & trimethopenem, ciprofloxacin, cefotaxime and cefepime. Although the prevalence of V. parahaemolyticus was high in raw fishes compared to other studies, antimicrobial resistance rate of the isolates was relatively low. These results could be useful information for risk assessment of V. parahaemolyticus in raw fishes.