This study examines the effect of SPA store experience on formation of customer equity. Here we distingush dimension of store experience to sensory, affective, behavioral, intellectual, and relational experience and dimension of customer equity to value, brand, relationship equity. To verify relationship between dimension of store experience and customer equity, as well as loyalty, we use structural equation modeling. As a result, relationship between variables have a significant effect on each other exclude hypotheses such as relationship between affective experience and value equity and relationship between behavioural experience and relationship equity. The result indicates that reinforcing SPA store experience likely to have positive impact on formation of customer equity and loyalty strength.

This study examines the effect of SPA store experience on formation of customer equity. Here we distingush dimension of store experience to sensory, affective, behavioral, intellectual, and relational experience and dimension of customer equity to value, brand, relationship equity. To verify relationship between dimension of store experience and customer equity, as well as loyalty, we use structural equation modeling. As a result, relationship between variables have a significant effect on each other exclude hypotheses such as relationship between affective experience and value equity and relationship between behavioural experience and relationship equity. The result indicates that reinforcing SPA store experience likely to have positive impact on formation of customer equity and loyalty strength.

This study explores the moderating role of perceived sponsor motive and event-self congruence on the relationship between event-sponsor congruence and attitude toward sponsor in CSR advertisingcampaigns. A scenario based 2 (event-sponsor congruence: high/low) ⅹ 2 (perceived sponsor motive: selfish/altruistic) ⅹ 2 (event-self congruence: high/low) between-subjects design experiments are conducted for the test of hypotheses. According to the results of ANCOVA, consumers' perceived sponsor motive and event-self congruence are shown to moderate the effect of event-sponsor congruence on consumers' attitude toward sponsor. And, when the consumer perceives sponsor motive as ‘altruistic’ and event-self congruence as ‘high’, the difference of attitude toward sponsor between the high group and low group of event-sponsor congruence perception is the lowest.

The mulberry white caterpillar, Rondotia menciana, belongs to the lepidopteran family Bombycidae, in which the domestic silkworm, Bombyx mori is included. In this study, we describe the complete mitochondrial genome (mitogenome) sequences of the species in terms of general genomic features and characteristic features found in the A+T-rich region. The 15,364-bp long genome consisted of a typical set of genes (13 protein-coding genes, two rRNA genes and 22 tRNA genes) and one major non-coding A+T-rich region, with the typical arrangement found in Lepidoptera. Twelve of the 13 PCGs start with typical ATN codons, except for the COI, which begins with CGA. Twelve of the 13 PCGs have complete stop codon, except for COII, which ends up with a single T. The 360-bp long A+T-rich region harbored the conserved sequence blocks that are typically found in lepidopteran insects. Additionally, the A+T-rich region of R. menciana contained one tRNAMet-like structure, which has a proper anticodon and secondary structure.

We newly sequenced mitogenomes of five skippers belonging to Lepidoptera to obtain further insight into characteristics of butterfly mitogenomes and performed phylogenetic reconstruction using all available gene sequences (PCGs, rRNAs, and tRNAs) from 85 species in 19 families in eight superfamilies. The general genomic features found in the butterflies also were found in the five skippers: a high A/T composition (79.3% - 80.9%), dominant usage of TAA stop codon, similar skewness pattern in various levels, consistently long intergenic spacer sequence between tRNAGln - ND2 (64-87 bp), the ATACTAA motif betweent RNASer(UCN) and ND1, and characteristic features of the A+T-rich region (the motif ATAGA, varying length of poly-T stretch, and poly-A stretch). The start codon for COI was CGA in four skippers as typical, but Lobocla bifasciatus evidently possessed canonical ATG as start codon. Phylogenetic analyses mainly yielded the consensus superfamilial relationships ((((((Bombycoidea + Noctuoidea + Geometroidea) + Pyraloidea) + Papilionoidea) + Tortricoidea) + Yponomeutoidea) + Hepialoidea) with a high support for most nodes, confirming the validity of Macroheterocera and its sister relationship to Pyraloidea. Within Rhopalocera the familial relationships (Papilionidae + (Hesperiidae + (Pieridae + ((Lycaenidae + Riodinidae) + Nymphalidae))) were strongly supported, confirming invalidity of the superfamily Hesperioidea. On the other hand, superfamilial relationships among Noctuoidea, Geometroidea, and Bombycoidea and the familial relationships among Saturniidae, Sphingidae, and Bombycidae were dubious, requiring further representative taxon sampling.

We report the complete mitochondrial genome (mitogenome) of Apodemia mormo, which belongs to the lepidopteran family Riodinidae. The 15,262-bp long complete genome is comprised of 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and one major non-coding A+T-rich region, with the arrangement typically found in majority of Lepidoptera. The genes of A. mormo are interleaved with a total of 168 bp, which are spread over 16 regions and overlap in a total of 58 bp at eight locations. All tRNAs of the A. mormo mitogenome formed typical cloverleaf structure, except for tRNASer(AGN), which formed the truncated dihydrouridine arm. COI gene started with CGA, instead of canonical ATN as seen in other Lepidoptera. The 349-bp long A+T-rich region harbored the conserved sequence blocks, such as ATAGA motif, poly-T stretch, the conserved ATTTA sequence, and microsatellite A/T repeat that are typically found in Lepidoptera, but absent for tRNA-like pseudogene.

Materials are limited for pest control and chemical pesticides are prohibited in organic farming. Thus, the concept of pest control may not be effective for pest management in organic farm. As an alternative approach, landscape management is potentially effective and useful approach for preventing pathogen and insect pests in organic farm. It is needed to develop best practices utilizing functional biodiversity for pest management through landscape management. For this purpose, we investigated the effect of companion plants on species richness of insect pests and natural enemies in the field border area and in the crop plants (rice and soybean). In rice paddy fields, Lysimachiae foenum-graecum, Saururus chinensis were selected as effective companion plants on the paddy field levee for conserving Arachnidae as natural enemy and density suppression of insect pest such as Lissorphoptrus oryzophilus, Riptortus clavatus. As a result, damage rate of rice plant by insect pests was significantly lower (2.5%) and yield rate of healthy grains was significantly higher (8.7%) than control. In soybean fields, Marigold plants such as Tagetes patula, T. officinalis, increased Arthropod species richness and ratio of natural enemy group, and decreased ratio of insect pest group. As a result, yield (weight of 100 seeds) of healthy soybean was significantly increased (2.5%) than control. The 2nd infection rate from damage by R. clavatus was significantly lower in the treated plot than that in control. Thus, yield rates of healthy soybean in the control and the companion plant-treated plot were 79.0% and 84.3%, respectively, compared to conventional farming using chemical pesticides. Therefore, use of companion plants on field border was an effective practices for preventing pest insects and increasing yield in organic farming.

The brown planthopper (BPH), Nilaparvata lugens (Stål), is an important pest in rice paddies. Because BPH cannot survive under low temperature conditions (<12℃), its outbreak in temperature areas depends on migration from overwintering areas of BPH. Geospatial distribution of BPH overwinterng area would be changed under future climate. The objective of the present study was to assess potential areas for BPH overwintering in Asia under current and future climate conditions. To reduce model uncertainty, we explored an ensemble modeling approach using five species distribution models (SDMs) and climate data from five general circulation models (GCMs). For future projections, outputs from an SDM with five sets of GCM data were averaged into a single map. Then, five maps from individual SDMs were summarized to assess the potential overwintering areas of BPH. Overall, the BPH overwintering areas expanded northward under future climate conditions. The degree of expansion in overwintering areas differed by region. For example, the uppermost boundary of BPH overwintering areas shifted north about 150 Km in southern China by 2050s. In Japan, small areas in the southern Kyushu were projected as potential areas of BPH overwintering. These results indicated that migration of BPH into East Asia including northern China, Korea, as well as Japan would occur early in the growing season, which could result in considerable damages in rice production.

Panax ginseng, also known as Korean ginseng, has long been used as a broad tonic in Oriental medicine to augment vitality, health, and longevity, particularly in older people. This study investigated the effects of Korean red ginseng (RG) on bone loss in ovariectomized (OVX) mice. C3H/HeN mice (10-weeks-old) were divided into sham and OVX groups. OVX mice were treated with vehicle, 17β-estradiol (E2), RG (oral administration, 250 mg/kg/day), or RG (intraperitoneal administration, 50 mg/kg/every other day) for 6 weeks. Serum E2 concentration and alkaline phosphatase (ALP) activity were measured. Tibiae were analyzed using microcomputed tomography. Biomechanical properties and osteoclast surface level were measured. There was no significant difference in the degree of grip strength, body weight, uterine weight, mechanical property, tibiae length, or tibiae weight between the OVX and RG-treated groups. Compared with the OVX group, the serum ALP level was significantly lower in the RG-treated groups. Serum E2 levels and osteoclast surface levels did not change between the OVX and RG-treated groups. RG could not preserve trabecular bone volume, trabecular bone number, trabecular separation, trabecular thickness, structure model index, or bone mineral density of the proximal tibiae metaphysic. In conclusion, there was no definite effect of RG on OVX-induced bone loss in C3H/HeN mice.

Brucellosis is an important bacterial zoonotic infection worldwide responsible for economic losses in livestock industries which represents a considerable and increasing public health burden. The disease is caused by Brucella which possess the ability to invade and replicate within phagocytes and may result to chronic infection, leading to difficulty in medical therapy of the disease. The treatment for brucellosis employs conventional principles that have been applied for a long time and a combination of antibiotics is currently used due to low efficacy of monotherapies. However, vital effects with respect to health and safety are neglected. Nevertheless, the preventive methods and treatments for brucellosis using traditional medicine have not yet been thoroughly studied, hence, alternative therapies such as the use of natural plant extracts as traditional medicine that are safe, efficient and economical should be explored in order to identify candidates that eliminate complications due to brucellosis. Coptis chinensis Franch (Huanglian) is a traditional Chinese medicinal herb and its extract has been known to possess strong antibacterial activity. In this study, the antibacterial effects of C. chinensis Franch extracts, and the major components of the herb namely berberine and palmatine, were investigated on B. abortus. The C. chinensis Franch ethanol extracts (CCFE) showed bactericidal effects at 1,000 μg/ml concentration and berberine at 100 μg/ml concentration. However, C. chinensis Franch and its components did not affect invasion and intracellular growth of B. abortus in RAW 264.7 cells. In conclusion, C. chinensis Franch ethanol extracts, water extract and its major components such as berberine and palmatine would be a beneficial antimicrobial agent without affecting phagocytic pathway within macrophages, and further study for the precise mechanisms of antibacterial effect would be necessary.

The multi-tactile stimulation system was developed to analyze cognitive function characteristics. The developed system consists of three units; control unit, drive unit and actuator that simultaneously and separately supports the vibration, heat, and pressure stimulation. From the results, vibration stimuli have a wide frequency range of 0-300 Hz comprising 20 levels, and stimulation intensity comprising 40 levels. The control temperature and stimulation temperature used for heat stimulation comprise four and eight levels, respectively. Pressure stimuli in the range of 0-600 mmHg can be stimulated comprising 12 levels. Therefore, the developed stimulator can be used for integrative tactile perception and cognition studies on various vibration, heat and pressure sensations.

In this paper, we developed a power amplifier system for driving a solenoid coil. The developed system consists of three units; control unit, drive unit and coil. The control unit generates a drive signal of sinusoidal wave type, monitors an operating status of the drive unit and vibration characteristics of the coil. The drive unit drives a solenoid coil in response to command from the control unit. The coil was fabricated with the size of diameter in 1.5 cm and length in 1.7 cm using electric copper wire. 4 types of drive unit were tested with thermal and vibration characteristics of the coil using temperature sensor and 3-axis accelerometer, respectively. From the result, the developed system has many advantages with respect to simple control algorithm, small size, and operates stably up to 68 watts driving condition.

We newly sequenced mitochondrial genomes of Spodoptera litura and Cnaphalocrocis medinalis (Lepidoptera) to obtain further insight into mitochondrial genome evolution and investigated the influence of optimal strategies on phylogenetic reconstruction of Lepidoptera. Estimation of p-distances of each mitochondrial gene for available taxonomic levels has shown the highest value in ND6, whereas the lowest values in COI and COII at the nucleotide level, suggesting different utility of each gene for different hierarchical group when individual genes are utilized for phylogenetic analysis. Phylogenetic analyses mainly yielded the relationships (((((Bombycoidea + Geometroidea) + Noctuoidea) + Pyraloidea) + Papilionoidea) + Tortricoidea), evidencing the polyphyly of Macrolepidoptera. The tests of optimality strategies, such as exclusion of third codon positions, inclusion of rRNA and tRNA genes, data partitioning, RY recoding approach, and recoding nucleotides into amino acids suggested that the majority of the strategies did not substantially alter phylogenetic topologies or nodal supports, except for some familial relationship only in the amino acid dataset.

Glyphodes quadrimaculalis (Lepidoptera: Crambidae) feed on a root tuber of Cynanchum wilfordii (Asclepiadaceae) that is one of the most famous traditional medicines in Korea. The genus Glyphodes includes ~130 species distributed worldwide, so the complete mitochondrial genome (mitogenome) would be helpful for bio-identification, biogeographic studies, and multigene-based phylogeny. The 15,255-bp long G. quadrimaculalis genome comprises 37 typical genes and one large non-coding region, with the typical arrangement found in Lepidoptera. Of the 13 protein coding genes (PCGs), 12 begin with typical start codons found in insect mitochondrial PCGs, but the COI gene starts with atypical CGA. One of the noteworthy features of the genome includes the presence of a 51-bp long non-coding space sequence located between tRNAGln and ND2 that reveals high sequence homology (71.4%) to the neighboring ND2 gene, indicating the origin of the region by partial duplication of the ND2 gene.

We present the nearly complete mitogenome sequences of the garden chafer, Polyphylla laticollis manchurica, which is listed as an endangered species in Korea. The P. l. manchurica mitogenome, which includes unfinished whole A+T-rich region and a partial srRNA was 14,473-bp long, possessing typical sets of genes (13 PCGs, 22 tRNA genes, and 2 rRNA genes). Gene arrangement of the P. l. manchurica mitogenome was identical to the common one found in the majority of insects. The 5 bp-long motif sequence (TAGTA) that has been suggested to be the possible binding site for the transcription termination peptide for the major-strand was also found in the P. l. manchurica mitogenome between tRNASer(UCN) and ND1. As has been previously determined, the high A/T content was unanimously observed in P. l. manchurica in terms of A/T bias in the third codon position (73.5%) compared with the first (66.4%) and second codon position (66.2%), and a high frequency of A/T-containing codons (a total of 28.22% for TTA, ATT, TTT, and ATA). The PCGs encoded in major-strands are slightly T-skewed, whereas those of the minor-strand are A-skewed, indicating strand asymmetry in nucleotide composition in the Coleoptera including P. l. manchurica.

1-deoxynojirimycin (1-DNJ), a potent a-glycosidase inhibitor, has therapeutic applications in treatments of HIV, Gaucher’s disease, and diabetes. 1-DNJ has been extracted from natural sources (mulberry leaves) for therapeutic purposes; however, 1-DNJ ingredients are in limited supply and are costly to obtain on a large scale. Since certain strains of Bacillus and Streptomyces species reportedly produce 1-DNJ, they may serve as potential sources for high-yield 1-DNJ production. In this study, we obtained evidence for four bacteria that produce 1-DNJ in large quantities by high performance liquid chromatography and thin layer chromatography. Investigation of the effect of mulberry leaves powder concentration(1~5%), using the 1-DNJ high-production bacteria, provided evidence for microbial mass production of 1-DNJ.

We sequenced 17,329 bp of mitochondrial genome (mitogenome) of the black dwarf honey bee, Apis andreniformis (Hymenoptera: Apidae), that lacks ~200 bp of the A+T-rich region for the completion of the genomic sequence. The gene arrangement of A. andreniformis mitogenome is identical to that of A. cerana. However, the genome contains 5 additional tRNALeu(CUN) located 4 copies between tRNAMet and tRNAGln, and 1 copy between tRNAGln and tRNAAla, along with the typical sets of genes (13 protein-coding genes, 22 tRNAs, and 2 rRNAs) including regular tRNALeu(CUN) and the A+T-rich region (at least 923 bp). Only 1 copy of tRNALeu(CUN) differed by 1 bp from other 4 copies of tRNALeu(CUN). Each additional tRNALeu(CUN) is followed by nearly identical 68-bp long repeat sequence (95.6% identity). All 13 protein coding genes have typical start codons found in insect mitochondrial PCGs (2 ATA, 9 ATT, and 2 ATG).

기계이앙과 무논점파재배시 간단관개가 벼의 생육, 쌀수량과 미질에 미치는 영향과 물절약 효과를 구명하고자 2009년과 2010년에 시험한 결과는 다음과 같다. 관개량은 기계이앙이 801mm, 무논점파재배가 717mm로 기계이앙이 더 많았다. 관개방법별로는 기계이앙재배에서 관행관개가 899mm, 간단관개가 703mm이었고, 무논점파재배에서는 관행관개가813mm, 간단관개가 621mm이었다. 간단관개는 관행관개에비해 22~24%의 관개수를 절약할 수 있었으며, 이에 따른 단위 관개량당 쌀수량인 물생산성은 기계이앙과 무논점파재배모두 간단관개가 높게 나타났다. 초장, 간장, 수장은 간단관개와 관행관개에 차이가 없었으나, 분얼수는 간단관개에서 다소높았다. 수량 및 수량구성요소 모두 관개방법별 유의적인 차이가 없었으며, 완전미 비율, 단백질 함량도 유의한 차이가 나타나지 않았다. 이상의 결과에서 간단관개를 하여도 관행관개방식에 비해 벼의 생육 및 수량, 그리고 미질에 차이가 없었던 반면 물생산성을 높이는 결과를 보였다. 따라서 벼 재배시용수절약 측면에서 간단관개의 적용이 가능할 것으로 보인다.간단관개를 포함한 종합적인 재배기술을 연구하여 관개비용의절감이나 수량의 증대 같은 이익적 요소들이 제공된다면 농가에서도 관심을 갖게 될 것으로 생각된다.