The traditional use of insects as food continues to be widespread in tropical and subtropical countries and to provide significant nutritional, economic and ecological benefits for rural communities. Specially, Bee brood serves as a food source to humans in many countries although limited data exists concerning its nutrient composition. Bee brood (pupa and larvae) were analyzed for Carbohydrate, Saturated fatty acid, Cholesterol, protein, fat, fiber, minerals, and vitamins. Bee brood was high in protein(46.4%～46.73%), fat(18.84%～ 20.75%),carbohydrate(24.66 %～35.79 %), Folic acid(222.30 ㎍/100g), and vitamins. Differentially, folic acid had been contained by high density in pupa of drone. While low in iron, bee brood was a good source of folic acid, and carbohydrate. The fat was composed mostly of saturated and mono-unsaturated fatty acids. The present data suggest bee brood to be an excellent source of many valuable nutrients including energy, amino acids, many essential minerals, and B-vitamins. These data suggest bee brood could be a valuable source of nutrients to various populations.

본 실험에서는 Ardisia속 자생식물의 온도 및 습도 변화에 따른 온열환경을 조사함으로써 자생식물의 이 용에 가능한 기초 자료를 제공하고자 실시하였다. 가로 2m, 세로 1.3m, 높이 1.8m, 총 부피가 4.68m3의 밀 폐된 유리 챔버 내에 온도 조건 변화에 따른 실내공 간의 온도 및 습도변화를 측정한 결과, Ardisia속 식 물에 의한 실내온도의 변화는 24oC 이상의 고온에서 는 식물이 없는 상태에 비해 감온 효과가 있었으며 3 종의 식물 간 큰 차이는 보이지 않았다. Ardisia속 식물 중에서 백량금이 온도 변화에 따른 습도 변화가 가장 민감하게 일어나 13%의 습도 변화를 보였고 57.3±3.1%로 비교적 높은 습도를 보였는데, 이에 비해 자금우는 8%, 산호수는 9%의 습도 변화를 보였고 자 금우와 산호수 모두 대부분의 낮 시간 동안 50~55% 의 실내 적정습도 범위를 유지했다. 식물체의 실내 발 열체로서의 열성능 평가 결과, 실내 온도를 28oC에서 26oC로 감온 시에는 식재에 의한 냉방 효과가 비식재 공간에 비해 7.5~13.6배 높았고, 적정온도 이상의 고 온에서는 자금우에 의한 냉방 효과가 백량금과 산호수 에 비해 더 좋았으며 적정온도를 유지하는데 효과적이 었다. 실내 저온 조건에서는 비식재 공간이 식물을 배 치한 경우보다 높은 열량을 나타내 식물이 저온 조건 에서 냉방효율을 낮추므로 적정온도를 유지하는데 효 과적이었다. 이로서 Ardisia속 식물은 고온의 실내에서 주위의 온도를 저하시키고 적정온도를 유지시키는데 효 과적임을 알 수 있었다.

As a result of inquiring into the analysis consequent on a symptom by research subject, it was found that there were 1 paper (1.5%) which did research for the purpose of rehabilitation of the general public, 26 papers (38.8%) targeting diagnosis-related groups (DRG), and 40 papers (59.7%) targeting social consideration subject. Also, as a result of the inquiry about the frequency & number of times of horticultural therapy program implementation, it was found that 49 papers (73.1%) implemented one time a week, and 45 papers (67.2%) were surveyed as the highest by conducting a total of 11~20 sessions of horticultural therapy programs, In the analysis of horticultural therapy activities by type, plant cultivation activity was found to be the most 506 times, accounting for 41.5%, followed by 297 time crafts activity (24.4%), 213 time floral decoration activity (17.5%), and 203time others activities (16.7%). In cultivation activity, soil-using cultivation activity (25.1%) was found to be lower than the proportion (74.9%) of soiless cultivation (16.4%), crafts activity (24.4%), floral decoration activity (17.5%) and other activities (16.7%). The most used plants in a restricted place like a hospital were found to be in the order of Hedera helix, Chamaedorea elegans, Succulent plant, Syngonium podophyllum, Neofinetia falcata HU, Hoya carnosa (L.f.) R.Br.), Rosmarinus officinalis, and Spathiphyllum spp.

The study was conducted to understand the relationship among university students’ interest in horticulture, psychological well-being and social development and the influence of measured variables. In order to collect data, the surveys were executed by convenience sampling on university students attending four-year universities in Daegu and Gyeongbuk regions of South Korea during the period from July 3 to July 17, 2016. A total of 307 survey results which measured the university students’ interest in horticulture, psychological well-being and social development were analyzed. The results showed that there are differences in the interest in horticulture, psychological well-being, and social development depending on the students’ general characteristics. According to the results analyzed by t-test and one-way ANOVA, the interest in horticulture depending on gender, religion, academic marks, family life satisfaction and school life satisfaction had significant differences showing high interest for the students with religion, good academic marks, high family life satisfaction and school life satisfaction as well as female students. Psychological well-being showed significant differences in academic marks, family life satisfaction and school life satisfaction, while social development displayed significant differences in gender, grade, academic marks, family life satisfaction and school life satisfaction. According to the results analyzed by correlation analysis, there was a significant correlation among university students’ interest in horticulture, psychological well-being and sociality development. Furthermore, regression analysis verified that university students’ interest in horticulture has a positive influence on psychological well-being and social development. The results of this study implies that university students with a higher interest in horticulture have higher levels of psychological well-being and social development.

Background : Acanthopanax sessiliflorus (Rupr. et Maxim) Seem, belonging to the Araliaceae family, is widely distributed in Korea, China, and Japan. The plants belonging to Acanthopanax species are traditionally used in Korea as anti-rheumatoid arthritis, anti-inflammatory and anti-diabetic drugs and are recognized to have ginseng-like activities. A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed and validated for independent analysis of major compounds and chlorogenic acid in A. sessiliflorus fruits. Chlorogenic acid was reported that prevent cancer and cardiovascular disease in vivo. Also, it has antioxidant effect in vitro test. In the previous experiment, chlorogenic acid were found in A. sessiliflorus fruits. This study was performed to identification of the major compounds and investigate the method validation for the determination of chlorogenic acid in A. sessiliflorus fruits. Methods and Results : Three major compounds were recorded on a Varian Unity Inova AS-400 FT-NMR spectrometer and analyzed by the new HPLC analysis method. HPLC analysis was carried out using an Waters e2695 and PDA detector. The new analyasis method was validated by the measurement of intra-day, inter-day precision, accuracy, limit of detection (LOD, S/N=3), and limit of quantification (LOQ, S/N=10) of chlorogenic acid. The results showed that the correlation coefficient (R2) for the calibration curves of chlorogenic acid was 0.997 in terms of linearity. The limit of detection (LOD) and limit of quantification (LOQ) were 0.565 ㎍/ml and 2.88 ㎍/ml, respectively. There was no interfering peak observed each other and HPLC system was suitable for analysis showing goodness of peak and high precision. Conclusion : This method is suitable to detect and quantify major compounds in A. sessiliflorus fruits. Furthermore, the result will be applied to establish chlorogenic acid as an standard compound for A. sessiliflorus fruits.

Background : Allergy is a common disease caused by type I hypersensitivity reaction of the immune system. Unfortunately, there is no proper treatment for allergy. Therefore, the discovery of therapeutic drugs for allergy is essential. In this study, the crude extracts of 56 plant parts were screened for anti-allergy effects in RBL-2H3 cells. Methods and Results : IgE-sensitized RBL-2H3 cells were individually treated with 56 extracts of medicinal herbs at the final concentration of 20 ㎍/㎖ and stimulated with the antigen DNP-BSA. β-Hexosaminidase release, interleukin-4 (IL-4) secretion, and cell viability in the sample treated cells were measured. Among the tested samples, extracts from the root of Polygonatum stenophyllum Maxim., aerial part of Acer triflorum Kom., and leaf of Pyrus pyrifolia var. culta (Makino) Nakai showed inhibitory effects on β-hexosaminidase release. The aerial part of Peucedanum japonicum Thunberg and seed of Panax ginseng C.A.Mey. showed suppressive activities on IL-4 secretion. All of the extracts were not cytotoxic at the tested concentration. Conclusion : From the result, six extracts including Polygonatum stenophyllum Maxim. (root) and Acer triflorum Kom. (aerial part) inhibited both β-hexosaminidase release and IL-4 secretion in IgE-sensitized RBL-2H3 cells. The use of these extracts for developing anti-allergy materials is suggested.

Mungbean (Vigna radiata) is a fast-growing, warm-season legume crop that is primarily cultivated in developing countries of Asia. We constructed a draft genome sequence of mungbean to facilitate genome research into the subgenus Ceratotropis and to enable a better understanding of the evolution of leguminous species. The draft genome sequence covers 80% of the estimated genome, of which 50.1% consists of repetitive sequences. In total, 22,427 high confidence protein-coding genes were predicted. Based on the de novo assembly of additional wild mungbean species, the divergence of what was eventually domesticated and the sampled wild mungbean species appears to have predated domestication. Moreover, the de novo assembly of a tetraploid Vigna species (Vigna reflexo-pilosa var. glabra) provided genomic evidence of a recent allopolyploid event. To further study speciation, we compared de novo RNA-seq assemblies of 22 accessions of 18 Vigna species and protein sets of Glycine max and Cajanus cajan. The species tree was constructed by a Bayesian Markov chain Monte Carlo method using highly confident orthologs shared by all 24 accessions. The present assembly of V. radiata var. radiata will facilitate genome research and accelerate molecular breeding of the subgenus Ceratotropis.

국내 쌀 품종의 아밀로오즈 함량에 따른 총 11종 쌀가루의 아밀로오즈 함량, 손상전분, 수분함량, 수분흡수지수(WAI), 수분용해지수(WSI), 입도분포 및 호화특성을 분석하였다. 국내 메성 쌀 품종 쌀가루의 아밀로오즈 함량은 9.95-21.52, 중간찰성은 11.69 및 찰성은7.09%의 아밀로오즈 함량을 나타냈으며, 유색미 메성품종은 17.06-18.36% 및 유색 찰성 품종은 7.04%의 아밀로오즈 함량을 나타내는 것으로 확인되었다. 국내 쌀품종 쌀가루의 수분함량은 7.19-13.89%를 나타내었고,수분흡수지수(WAI) 및 수분용해지수(WSI)의 경우 각각 남일 및 동진찰이 가장 높게 나타났고, 수분함량이 높은 시료의 경우 손상전분 함량이 낮은 것을 확인하였다. 국내 11품종의 쌀가루 입도 분포는 27.61- 189.67 μm를 나타내었으며 일반 메성 신동진 이 189.67 μm로 가장높은 입도를 나타내었으며, 일반메성 품종인 남일이27.61 μm로 가장 낮은 입도분포를 나타내는 것으로 확인되었다. 또한 본 연구결과 쌀가루의 수분함량이 낮은시료가 전분입도가 낮으나 손상전분 함량이 높은 것으로 확인되었으며, 일반 메성 품종의 경우 수분함량 및입도가 작은 시료는 최고 점도 및 최종점도가 높고, 유색메성품종의 경우 수분함량 및 입도가 작은 시료는 점도가 낮은 결과를 나타냈다.

Gibberellic acid (GA) is a well-characterized plant hormone, which plays a critical role in various plant growth and development. including stem elongation, floral indcution and seed development. GA is known to cause enlargement of ripening fruits and, especially in grapevines, GA shows a unique function: the induction of seedlessness in seeded grape varieties. However, despite extensive previous studies about GA, there has been no clear verification of the mechanism that induces seedlessness in grapes. To understand how GA treatment results in artificial parthenocarpy of seeded grapes at molecular levels, we analyzed transcriptional changes in seeded grapes with and without GA application in various inflorescence developmental stages using RNA-seq. At 14 days before flowering (DBF), seeded grapes were treated with 100 ppm GA and clusters were collected at three developmental stages: 7 DBF, full bloom, and 5 days after flowering (DAF). Of a total of 28,974 genes that were mapped to grape genome reference sequences, 7,013 and 9,064 genes were up- and down-regulated, respectively, in the GA-treated grape as compared to the non-GA-treated control at 7 DBF, full bloom, and 5 DAF. Clustering analysis revealed that these genes could be grouped into 9 clusters with different expression patterns. We also carried out functional annotation based on gene ontology categories. There were significant differences in the expression of the GA and auxin-related gene families. These findings expand our understanding of the complex molecular and cellular mechanisms of GA-induced parthenocarpy of grapes and provide a foundation for future studies on seed development in grapevines.

The amount of genetic variability of a species is essential for its survival and adaptation in different environments, and studies of genetic diversity using molecular markers are necessary to understand the genetic structure of a population and to orientate effective strategies of germplasm conservation. The aim of current study was to determine the SSR markers that can be used rapidly and reliably to evaluated the pepper of Bulgaria landraces, and applied the markers to assement of introduce genetic diversity of the pepper germplasm. We used 22 polymorphic microsatellite markers to analysis of genetic diversity within 61 pepper collection of Bulgaria landraces germplasm, all SSR primers pairs produced 80 polymorphic and reproducible amplification fragments. An average value of polymorphic information contents (PIC) were 0.334 with a range of 0.061 to 0.63. The mean values of observed (HO) and expected heterozygosity (HE) were 0.383 and 0.154, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into three distinct groups, which was the landrace, moderate and wilde type, genetic distance (GD) value was 0.540. An average day of flowering time was 53 days with a range of 45 to 60 days. The everage od fruit length and width were 9.38cm with a range 2.1 to 23.6cm, and 3.51cm with a range 0.6 to 8.9cm, respectively. Molecular data were complemented with morphological measurements according to the descriptor list for the pepper collection of Bulgaria landraces germplasm.

A total of 17 types of rice flour was collected in the market and investigated with respect to their starch and pasting characteristics. Their water contents, water holding capacities, solubilities, swelling power, amylose contents, damaged starches, particle sizes and pasting characteristics were measured. The water content and water holding capacities of various types of rice flours collected in the market were 7.64-14.97% and 95.22-232.94%, respectively. Their solubilities and swelling power were 1.97-37.40% and 1.33-5.76, respectively. Various types of rice flour had 32.11-305.67 μm particle sizes. Among the various types of rice flour collected in the market, the lowest starch damaged content was 3.17%. The peak viscosities of the samples were 17.74-295.95 RVA. Among the rice flours that were collected in the market, the lowest pasting temperature was 69.72℃. As a result, it was shown that rice flour types with high starch damage contents tend to have higher expressed water holding capacities. Moreover, the samples with lower particle sizes tended to have final viscosities, and pasting temperatures.

Oat flour was purchased and investigated for quality characteristics of oat bread. Antioxidant and flour pasting properties of oat flour, and water content, weight, volume, Hunter color value, texture of bread were measured. DPPH radical scavenging capacity (%) of oat extract was 68.49%. Pasting temperature of oat flour ranged between 66.60~circC and 70.93~circC . Flour pasting properties of sample added with 10 and 30% oat flour was shown similar results compared with wheat flour. Final viscosity of sample was increased by adding concentration of oat flour (up to 311.65 RVA). Water content (%) of bread was shown increasing trend by adding oat flour. Weight of bread loaf with 100% oat flour was shown higher score than other samples. Volume of loaf by adding oat flour of 10, 30, and 50 % ratio was 550, 450, and 388 mL, respectively. The Hunter color L* values of bread by adding oat flour was dark compared with wheat bread. Color value of bread added 10% oat flour was similar results with L* values of bread with wheat flour. The bread added with 20 and 30% oat flours had the higher hardness, gumminess, and chewiness compared with other samples. Cohesiveness of the bread with wheat flour showed higher than that of bread added with oat flour and increased by storage period. The growth of total viable cell was inhibited depending on the concentration of oat flour during storage.

Specialty barley extracts were prepared and investigated for its antioxidant activity and biological activity. Hunter L* values of the Iksan 86 extracts had higher than that of the Iksan 87 and Zasoojeongchal extracts. The extraction yields of Iksan 86, Iksan 87, and Zasoojeongchal was 8.08, 6.62, and 7.30%, respectively. The contents of total phenolic compounds of the Iksan 86, Iksan 87, and Zasoojeongchal extracts were 16.24, 15.51, and 13.95 GAE mg/g of sample, respectively. The DPPH radical scavenging activity of Iksan 86, Iksan 87, and Zasoojeongchal extracts were 50.00, 33.27, and 7.56% at a 500 ppm, respectively. The samples showed an inhibition of xanthin oxidase. ACE inhibition effect of specialty barley extracts, Iksan 86, Iksan 87, and Zasoojeongchal, was 39.81, 41.06, and 27.78%, respectively. Tyrosinase inhibition rates (%) of Iksan 86, Iksan 87, and Zasoojeongchal extracts were 26.21, 24.57, and 20.00%, respectively. Results indicated that specialty barley extracts possesses various biological activities including antioxidative capacity, xanthin oxidase inhibition activity, angiotensin converting enzyme inhibition activity, and tyrosinase inhibition activity.