This study was carried out to determine the sex of genomic and embryonic DNA using polymerase chain reaction(PCR). Bovine specific(216bp) and Y chromosome speicific DNA primers(l4lbp) were synthesized and tested for sexing. Bovine embryos used in this study were produced by in vitro fertilization. Few blastomeres for PCR were bisected by nicromanipulator and demi -embryos were cultured in TCM 199 medium containing 0.1% of solcoseryl. The results obtained were as follows; 1. Average optical density of genomic DNA extracted from blood of Hanwoo was 1.79 0.14. 2. 2. The ratio of the demi-embryos developed to blastocyst was 62.1 and 81.9% in morula and blastocyst, respectively. 3. When DNA of 2~4, 5~10 and more than 11 blastomeres was amplified with Y chromosome specific DNA primer by PCR, appreance rate of Y specific DNA band was 16.7, 46.2 and 40.0%, respectively. At least 5 to 10 blastomeres were required to determine the sex of embryos. 4. The rate of demi-embryos developed to blastocyst was 73.3% in TCM 199 medium supplemented with 0.1% solcoceryl. but 55.6% in control.

SUMMARY I. 서 론 II. 재료 및 방법 III. 결 과 IV. 고 찰 V. 적 요 VI. 인용문헌

A few archeological excavation of Buddhist temple sites of Koguryo were reported with information of site arrangement. The interpretation of such information is very important not only for the explanation of Korea early Buddhist architecture but also for the development of East Asian cases in general since no archeological evidences of the same period were found in other countries such as China. Though the investigation of the four temple sites this paper attempt to identify their date of construction and the process of change. The study depended much on comparative studies of the change of site elements such as pagoda, image hall., corridor, and other buildings. The study could conclude that the site of SangO-Ri must be the earliest case which was followed by ChungRung-Sa, which was built in around 427AD. The site of ToSung-Ri was the case which was the work of the rate 5th century. Such process of the development of Buddhist architecture in Koguryo unveils the fact that the process of change was a continuous flow toward a consistent goal of change that had much to do with the contemporary religious situation.

The study is about the process of modernization of conventional rural houses in Kyung-gi province. It alms to identify the pattern of change, particularly focusing on the changes of the site plan and plan. The site of study is Samga Village, in YongIn County, near Seoul. The study discusses the process of modernization during the three decades from 1960 to 1980. The basic information was obtained through field research and site analysis, and the study relies predominatly on observational and statistical analysis. Four developmental stages can be clearly identified which are ; (1) Traditional type, (2)Modified-traditional type, (3) Saemaul type, and (4) The type of urban houses. The analysis was done through identification of changing site plan and plan. It is interesting to note that the changes of these elements show a certain pattern of sequential steps which also can be classified according to the four developmental stages mentioned above. The pattern of site design was consistantly maintained while plan and building system became very different However, the process of modernization of Korean rural houses was the process of producing a certain type of architecture that is not universal but uniquely Korean.

We analysed 398 kinds of processed foods sold on market - 43 kinds of ramyun, 67 kinds of bread, 104 kinds of cookies, 5 kinds of pizza, 28 kinds of sausage, 31 kinds of steamed fish cake, 4 kinds of tomato ketchup, 30 kinds of canned goods, 82 kinds of beverage and 4 kinds of danmoogi - to know mineral content such as P, Ca, Mg, Na and K. We used atomic absorption spectrophotometry for this study and the results were as follows; 1) P content was 204.24㎎% in sausages, 101.80㎎% in pizza, 67㎎% in canned fish, 44.50㎎% in canned vegetable, 38.62㎎% in ramyun and 32.10㎎% in steamed fish cake. Sausage had the highest P content showing almost 6 times as much as steamed fish cake. There was little difference between ramyun and steamed fish cake. 2) Ca content was 115.00㎎% in pizza, 106.94㎎% in steamed fish cake, 100.59㎎% in sausage, 91.25㎎% in canned vegetable, 79.84㎎% in bread and 78.97㎎% in ramyun. 3) Mg content was 555.16㎎% in steamed fish cake, 476.14㎎% in cookies, 436.75㎎% in ketchup, 394.00㎎% in canned vegetable, 346.95㎎% in ramyun and 341.40㎎% in canned fish. Steamed fish cake was highest in Mg content and there was little difference between ramyun and canned fish. 4) Na content was 730.49㎎% in ramyun, 680.25㎎% in danmoogi, 548.43㎎% sausage, 463.50㎎% in canned vegetable, 449.25㎎% in ketchup, 401.29㎎% in steamed fish cake and 366.00㎎% in pizza. Ramyun had the highest Na content. 5) K content was 302.30㎎% in pizza, 280.25㎎% in ketchup, 219.68㎎% in sausage, 219.2㎎% in canned fish, 183.75㎎% in canned vegetable, 166.49㎎% in ramyun and 122.77㎎% in cookies. Pizza had the highest K content.

Patterns in the outbreaks of senile diseases were investigated on 1, 264 workers by special examination among the 43, 210 industrial workers by the screening test performed in Kwang-ju and Chon-nam Branch of Korean Industrial Health Association from January 4 to December 31 in 1991. The results are summarized as follows : 1) Prevalence rate of senile diseases of industrial workers was 2.9%. And prevalence rate according to the kinds of senile diseases was observed in the order of hepatitis (0.9%), hypertension(0.7%), diabetes mellitus(0.5%), anemia(0.4%). 2) Prevalence rate of complicated senile diseases of industrial workers was 0.5%. And prevalence rate according to kinds of complicated senile diseases was observed in the order of others hypertension and arteriosclerosis(0.1%), HBV and others chronic hepatitis(0.1%), essential hypertension and arteriosclerosis(0.03%), iron deficiency anemia and leukopenia(0.03%).

This experiment 'was conducted to investigate the grazing cattle's palatability by the method of grazing time on the pasture dominated orchardgrass and on the pasture dominated perennial ryegrass from June to October, 1987. The experiment was carried out

According to “Korea Environment Corporation (KECO): Statistical data of specified waste generation and treatment,” the amount of designated waste generated in 2015 was 4,691,532 tons, which is 51.7% higher than the 3,092,591 tons generated in 2005, and the annual output is steadily increasing by 3.25%. Therefore, in order to properly manage the specified wastes, it is necessary to divide them according to the type of industry and waste type and to evaluate the trend of specified waste generation in advance and establish a specified waste management plan. However, due to the statistical data collection and administrative procedures, the timing of the publication of national statistical data is somewhat delayed after the actual generation of specified waste. Since the “National Waste Comprehensive Plan” assumes that GDP and manufacturing output will increase by 4% every year and predicts the amount of specified waste generation in the future, there is a great deal of pressure to use this in the actual field. In this study, we analyzed the correlation between the production index, which is an economic index indicating the change in the output of the products produced by the industry, and the specified waste generation amount. Based on the actual production index, the specified waste statistics for 2017 were predicted and compared with the estimated amount and the actual amount for the 2010 ~ 2015 period.

급격한 경제적･사회적 변화에 따라 제조업에서 발생하는 폐기물의 종류와 발생량 역시 다양하고 복잡하게 변화하고 있다. 사업장폐기물은 위해성에 따라 사업장일반폐기물과 지정폐기물로 분류되며, 지정폐기물은 사업장폐기물 중 주변 환경을 오염시킬 수 있거나 오염성 폐기물 등 인체에 위해를 줄 수 있는 유해성분을 지니고 있는 폐기물로써, 적정한 처리를 위해 관리 및 감시 등의 조치가 필요하다. 이러한 이유로 폐기물관리법에서는 올바로시스템을 통하여 지정폐기물의 발생량과 처리량 통계조사를 1년마다 실시토록 하고 있다. 따라서 본 연구에서는 지정폐기물 통계조사를 효과적으로 활용하기 위하여 물질의 흐름을 분석하여 정량적이고 체계적으로 평가하였다. 올바로시스템를 통하여 통계자료를 수집하였으며 2007년에서 2012년 사이의 자료는 통계방식이 다소 상이함으로 2005년과 2015년에 대하여 발생량과 처리량을 비교 분석하였다. 이에 본 연구에서는 국내 제조업에서 발생하는 지정폐기물에 대한 업종별 물질흐름분석을 통해 주요 발생물질과 발생원을 파악하고 지정폐기물 관리 및 정책 수립의 기초자료를 제공을 목적으로 한다.

감귤의 색소 중 널리 분포되어있는 flavonoid와 limomoid 그리고 carotenoid들의 항암성을 비롯한 생리활성에 대한 연구결과들이 과일의 기능성식품으로서 가치를 재조명하고 있다. 본 연구에서는 기능성이 있는 색소물질에 대한 분석을 통하여 색깔돌연변이 감귤의 품종등록에 필요한 데이터를 제공하고 품종의 신규성과 유용성을 제공하여 품종화에 기여하고자 수행되고 있다. 연구를 위한 궁천조생의 가지변이인 붉은색을 띄는 자연발생적 돌연변이과실을 확보하였다. 색깔돌연변이체 특성을 분석한 결과 과실의 종·횡경, 무게, 과피두께, 가용성 고형물 및 유기산함량은 유의차(p<0.025)가 없었다. 변이체의 L(명도)는 46.5, a(적녹도)9.2, b(황청도)22.6 으로 대조군보다 +2.2 적녹도와 +6.6의 황청도가 측정되었다. 과피의 80℃열수추출물과 메탄올추출물의 DPPH radical scavenging test에서는 Vtiamin C 만큼의 좋은 활성을 나타내었다. 대조군과 색깔돌연변이체의 과피 FT-IR분석 결과 변이체가 protein/amide Ⅰ과 Ⅱ(1,500-1,700cm-1) 지역에서 빠른 감소를 보여주는 것을 제외하고는 유사하였다. HPLC를 이용한 분석에서는 변이체의 성숙한시기 과피에서의 violaxanthin함량이 대조군보다 높은 함량을 나타내었다. 현재 색깔돌연변이체를 대상으로 분석된 결과와 계획 중인 flavonoid분석등의 기능성분석을 통하여 변이체가 갖고 있는 우수한 형질을 조사하여 육성품종으로서의 가치를 조명하고자한다.