농촌진흥청 국립식량과학원에서 논 조건 적응 국수용 품종의 제분율 증가와 붉은곰팡이병 저항성 증진을 목적으로 1996년도에 백립계 춘파 다수성인 Bacanora88을 모본으로 하고, 현재 국내 최대 재배되고 있는 금강밀을 부본으로 인공교배하여 YW2948 조합을 육성하였다. 경기도 연천에서 집단재배 후 계통을 전개하여 초형과 수형이 양호하며 숙기가 빠르고 내한성이 강한 계통인 YW2948-B-B-1-1-1-1-1-1을 2005년과 2006년도 2년간 생산력검정을 거쳐 ‘익산320호’로 계통명을 부여하고, 2007년부터 3개년 동안 지역적응시험을 실시한 결과 숙기가 빠르며 백립계이고, 붉은곰팡이병에 중도 저항성을 나타냈다. 제분율이 높으며 밀가루와 국수 색택이 밝고, 식미감이 우수하여 2009년 농작물 직무육성 신품종심의회에서 ‘중모2004’로 명명하였다. 그 후 국립종자원의 재배심사를 거쳐 2013년 종자산업법제55조에 따라 품종보호 등록 원부에 등록되었다. 중모2004의 파성은 III형으로 양절형이고, 엽색은 녹색이며 이삭은 방추형이고, 종자는 중간크기로 백색이다. 출수기와 성숙기는 밭 조건 조건에서 각각 4월 24일과 6월 4일로 금강밀보다 1~2일 빠르고, 논 조건에서는 각각 4월 21일과 6월 2일로 2일 빠르다. 내한성은 금강밀보다 약하지만 수발아 저항성은 금강밀보다 강하고, 흰가루병과 붉은곰팡이병은 감수성이다. 중모2004의 제분율은 금강밀보다 약간 낮지만, 밀가루의 품질과 국수 가공특성이 비슷한 특성을 보였다. 천립중은 42.5 g으로 금강밀보다 약간 적은 소립이며, 지역적응성 시험에서 밭 조건 수량은 517 kg/10a으로 11% 감소하였고, 논 조건 수량은 534 kg/10a로 금강밀보다 3% 증수하였다.

농촌진흥청 국립식량과학원에서 수확시기가 빠른 국수용밀 품종 개발을 목적으로 1997년도에 조숙 계통인 SW86054 를 모본으로 하고 현재 국내 최대 재배되고 있는 금강밀을 부본으로 인공교배하여 SW97031 조합을 육성하였다. 경기도 연천에서 집단재배 후 계통을 전개하여 초형과 수형이 양호하며 숙기가 빠르고 내한성이 강한 계통인 SW97031-B-47-4-1-5-1-1을 2005년과 2006년도 2년간 생산력검정을 거쳐 ‘익산318호’로 계통명을 부여하고, 2007년부터 3개년 동안 지역적응시험을 실시한 결과 숙기가 빠르며 내한성이 강하고, 백립계이면서 수발아 중도저항성으로 생산안정성이 우수하였다. 밀가루와 국수 색택이 좋고 식미감이 우수하지만 단위면적당 수량 개선이 요구되어 2009년 농작물 직무육성 신품종심의회에서 ‘중모2003’으로 명명하였다. 그 후 국립종자원의 재배심사를 거쳐 2013년 종자산업법제55조에 따라 품종보호등록 원부에 등록되었다. 중모2003의 파성은 III형으로 양절형이고, 엽색은 녹색이며 이삭은 방추형이고, 종자는 중간크기로 백색이다. 출수기와 성숙기는 밭 조건 조건에서 각각 4월 24일과 6월 4일로 금강밀보다 1～2일 빠르고, 논 조건에서는 각각 4월 21일과 6월 2일로 2일 빠르다. 추위와 수발아저항성이 강하고, 흰가루병과 붉은곰팡이병은 감수성이다. ‘중모2003’의 제분율은 금강밀보다 약간 낮지만, 밀가루의 품질과 국수 가공특성이 비슷한 특성을 보였다. 천립중은 42.5g으로 금강밀보다 약간 적은 소립이며, 지역적응성 시험에서 밭 조건 수량은 555 kg/10a, 논 조건 수량은 485 kg/10a로 금강밀보다 4%와 7% 감수하였다.

국립식량과학원에서 수발아와 붉은곰팡이병 등에 대한 내재해성이 강한 국수용 밀 품종 육성을 목적으로 1997년도에 금강밀과 은파밀을 인공교배 한 F1을 모본으로 하고 다시 금강밀을 부본으로 인공교배하여 SW97005 조합을 육성하였다. 경기도 연천에서 집단재배 후에 계통을 전개하여 초형과 수형이 양호하고 내한성이 강한 계통인 SW97005-B-26-5-4-1-1-1을 2005년과 2006년도 2년간 생산력검정을 거쳐 ‘익산319호’로 계통명을 부여하고, 2007년부터 3개년 동안 지역적응시험을 실시한 결과 내한성이 강하고, 수발아 저항성이며 붉은곰팡이병에 중도저항성이고, 수량이 많을 뿐만 아니라 국수가공적성이 우수하여 2009년 농작물 직무육성 신품종심의회에서 ‘수안밀’로 명명하였다. 그 후 국립종자원의 재배심사를 거쳐 2013년 종자산업법제55조에 따라 품종보호 등록 원부에 등록되었다. 수안밀의 파성은 III형으로 양절형이고, 엽색은 녹색이며 이삭은 방추형이고, 종자는 중간크기로 적색이다. 출수기와 성숙기는 밭상태 조건에서 각각 4월 25일과 6월 4일로 금강밀보다 1일 빠르고, 논상태에서는 각각 4월 23일과 6월 4일로 동일하다. 내한성과 수발아 저항성이 강하고 붉은곰팡이병은 중도저항성이다. 수안밀의 제분율은 금강밀보다 약간 낮고, 단백질 함량과 회분함량이 낮아 밀가루 색이 밝고 단백질적 특성은 국수용에 적합하다. 천립중은 43.7 g으로 금강밀보다 약간 적은 소립이며, 지역적응성 시험에서 밭상태 수량은 594 kg/10a, 논상태 수량은 563 kg/10a로 금강밀보다 3%와 8% 증수하였다.

Fusarium head blight (FHB) is a major disease problem on wheat and barley around the world. F. graminearum produces trichothecenes mycotoxins such as nivalenol (NIV), deoxynivalenol (DON), and zearalenone (ZEA). The objectives of this study were to survey the natural occurrence of FHB and mycotoxins of 32 Korean wheat cultivars grown in 2011-2012 seasons at the National institute of crop science, Iksan, Korea. There was great deal of rainfall and high humidity during flowering time in May 2011. FHB incidence was counted by Fusarium infected spikes per square meter. The samples of 32 wheat cultivar were collected. The grain and flour samples were to analysis for DON and NIV by gas chromatography and ZEA by high performance liquid chromatography. The result showed that the average of FHB incidence(%) per square meter in 2011 and 2012 were 4.2%, 0.5% respectively. There were significant cultivar differences for FHB incidence ranged from 0% to 24% in 2011. All of 32 wheat cultivars contained 9-2088 ng/g for NIV and ten wheat cultivars contained 5.7-8.5 ng/g for ZEA. In addition, DON concentration of Tapdong, Shinmichal1, and Hanbaek were 217, 35 and 683 ng/g respectively. However, the grain and flour sample harvested in 2012 showed that lower FHB incidence and NIV concentration. These results showed that the 32 wheat cultivars harvested in 2011 were heavily contaminated with Fusarium mycotoxins (NIV, DON, ZEA).

Amplified fragment length polymorphism (AFLP) is one of molecular marker technique based on DNA and is extremely useful in detection of high polymorphism between closely related genotypes like Korean wheat cultivars. Six Korean wheat cultivar specific marker sets have been developed from inter simple sequence repeat (ISSR) analysis and we can identify the 13 Koran wheat cultivars form other cultivars using six that (Son et al., 2013). We used four combinations of primer sets in our AFLP analysis for developing additional cultivar specific markers in Korean wheat. Twenty-one of the AFLP bands were isolated from ACG/M-CAC primer combination and 19 bands were isolated from E-AGC/M-CTG primer combination, respectively. We used forty bands to design sequence characterized amplified region (SCAR) primer pairs for Korean wheat cultivar identification. Only one of 40 amplified primer pairs, C2, were able to use for wheat cultivar identification. The DNA band of 215bp length was amplified by C2 primer pairs in ten cultivars, Eunpa, Olgeuru, Gobun, Saeol, Milsung, Sinmichal, Jokyung, Sugang, Goso, and Joah. Then C2 primer was applied to these primer sets as newly SCAR marker, six cultivars are identifying from other cultivars, additionally. Finally, to use the C2 and six primer sets, 19 Korean wheat cultivars are identified.

“Sooan”, a winter wheat(Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Keumkang/Eunpa//Keumkang” during 1997. “Sooan” was evaluated as “Iksan319” in advance yield trial test in 2006. It was tested in the regional yield trial test between 2007 and 2009. “Sooan” is an awned, semi-dwarf and hard winter wheat, similar to “Keumkang” (check cultivar). The heading and maturing date of “Sooan” were similar to “Keumkang”. “Sooan” had similar test weight (817 g/L) and lower 1,000-grain weigh (43.7g) than “Keumkang” (817 g/L and 46.2g, respectively). “Sooan” showed resistance to winter hardiness and pre-harvest sprouting, which lower withering rate on the low ridge (0.2%) and rate of pre-harvest sprouting (0.2%) than “Keumkang” (9.2 and 25.8%, respectively). “Sooan” showed lower protein content (12.7%), SDS-sedimentation volume (42.0ml) and gluten content (10.5%) than “Keumkang” (13.1%, 57.8ml and 10.8%, respectively). It showed higher lightness (90.08) and lower redness (-1.11) and yellowness (8.80) in flour color than “Keumkang” (89.81, -1.15 and 9.46, respectively). “Sooan” showed higher lightness (81.34) of noodle dough sheet than “Keumkang” (80.28). “Sooan” exhibited lower hardness (3.84N) and similar springiness and cohesiveness of cooked noodles (0.91 and 0.65) compared to “Keumkang” (4.54N, 0.93, and 0.63, respectively). Average yield of “Sooan” in the regional adaptation yield trial test was 5.94 MT/ha in upland and 5.63 MT/ha in paddy field, which was 3% and 8% higher than those of “Keumkang” (579 MT/ha and 520 MT/ha, respectively).

“Joongmo2003”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “SW86054/Keumkang” during 1997. “Joongmo2003” was evaluated as “Iksan318” in advance yield trial test in 2006. It was tested in the regional yield trial test between 2007 and 2009. Its heading date was April 24 in upland and April 21 in paddy filed conditions, which were two days earlier than those of the check cultivar Keumkang, respectively, and Maturing date was June 4 in upland and June 2 in paddy field, one or two days earlier than those of Keumkang, respectively. “Joongmo2003” showed resistance to winter hardiness and pre-harvest sprouting, which lower withering rate on the low ridge (0.3%) and rate of pre-harvest sprouting (18.5%) than “Keumkang” (9.2 and 25.8%, respectively).“Joongmo2003” showed similar protein content (13.2%), lower SDS-sedimentation volume (54.0ml) and higher gluten content (11.7%) than “Keumkang” (13.1%, 57.8ml and 10.8%, respectively). It showed higher lightness (89.85) of flour and noodle dough sheet than “Keumkang” (89.81 and 80.28, respectively). “Joongmo2003” exhibited similar hardness (3.84), springiness and cohesiveness of cooked noodles (0.92 and 0.63) compared to “Keumkang” (4.54N, 0.93, and 0.63, respectively). The average grain yield in the regional yield trial was 5.55MT/ha in upland and 4.85 MT/ha in paddy field, which were 4% and 7% lower than those of the check cultivar, Keumkang, respectively.

“Joongmo2004”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Bacanora88/Keumkang//Keumkang” during 1996. “Joongmo2004” was evaluated as “Iksan320” in advance yield trial test in 2006. It was tested in the regional yield trial test between 2007 and 2009. Its heading date was April 26 in upland and April 23 in paddy filed conditions, and Maturing date was June 5 in upland and June 4 in paddy field, which were similar to Keumkang, respectively. It showed 830 in number of spikes per m2, 35 of grain number per spike, 44.9g of 1,000 grain weight, and 806g of test weight. “Joongmo2004” showed moderate to Fusarium head blight (Scab) in test of specific character although “Keumkang” is susceptible to scab. “Joongmo2004” had higher flour yield (75.5%) than “Keumkang” (72.5% and 0.42%, respectively). “Joongmo2004” showed similar ash (0.42%), lower protein content (12.3%), SDS-sedimentation volume (43.0ml) and gluten content (10.3%) than “Keumkang” (0.42%, 13.1%, 57.8ml and 10.8%, respectively). It showed higher lightness (89.97), redness (-1.38) and yellowness (10.76) in flour color than “Keumkang” (89.61, -1.15 and 9.46, respectively). “Joongmo2004” exhibited lower hardness (3.50N), similar springiness and higher cohesiveness of cooked noodles (0.92 and 0.65) compared to “Keumkang” (4.54N, 0.93, and 0.63, respectively). The average grain yield in the regional yield trial was 5.17MT/ha in upland and 5.34 MT/ha in paddy field, which were lower 11% and higher 3% than those of the check cultivar, Keumkang, respectively.

“Joongmo2012”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Sinmichal/Keumkang” during 2006. “Joongmo2012” was evaluated as “Iksan357” in advance yield trial test in 2010. It was tested in the regional yield trial test between 2011 and 2013. Its heading date was May 2 in upland and May 1 in paddy filed conditions, and Maturing date was June 8 in upland and June 8 in paddy field, which were similar to Keumkang, respectively. It showed 628 in number of spikes per m2, 34 of grain number per spike, 47.6g of 1,000 grain weight, and 762g of test weight. “Joongmo2012” showed susceptible to Fusarium head blight (Scab) in test of specific character although “Keumkang” is susceptible to scab. “Joongmo2012” had lower flour yield (68.4%) than “Keumkang” (73.5%, respectively). “Joongmo2012” showed similar ash (0.40%), lower protein content (11.7%), SDSsedimentation volume (39.8ml) and gluten content (8.1%) than “Keumkang” (0.41%, 12.7%, 55.8ml and 9.2%, respectively). It showed higher lightness (92.70) in flour color than “Keumkang” (91.51, respectively). “Joongmo2012” exhibited lower hardness (3.19N), similar springiness and higher cohesiveness of cooked noodles (0.90 and 0.66) compared to “Keumkang” (4.21N, 0.90, and 0.62, respectively). The average grain yield in the regional yield trial was 5.48MT/ha in upland and 4.47 MT/ha in paddy field, which were higher 5% and similar than those of the check cultivar, Keumkang, respectively

Stem rust of wheat caused by Puccinia graminis f. sp. Tritici is historically one of the most important diseases of crops worldwide. The objective of this study was to screen the growth habits and yield of wheat accessions in Korea winter condition. We collected ninety stem rust resistant germplasms from USDA-Grain and planted for the characteristics of growth and yield in 2013. We measured the growth characteristics such as the plant type, leaf color, heading date, stem length, spike length, awn length, number of spikelet, number of tiller, and 1,000 kernel weight (g). Early maturity is most important wheat breeding target in double cropping system such as Korea. Heading dates of ninety cultivars ranged from May 17 to June 7. The Keumkang control cultivar headed on May 23. Heading dates of seventeen accessions had as early as Keumkang control (May 23). The numbers of semi-erect and intermediate type for growth habit were 45 and 33 respectively. The 1000 kernel weights were also measured. Mean 1000 kernel weights ranged from 16.7g (Webster) to 41.8g (123 A1C2). The average of stem length was 100.2cm and Keumkang was 58.4cm. These results showed that A4849-60P-7P-1P (Chile), MG 31492 (Ethiopia), 1338.F.6.C.3.B (Kenya), 1203 A1C2 (Kenya), and II-90-20/3-1R-1M- 3R (Mexico) were best lines for agronomic characteristic such as early heading date and grain yield

Characteristics of agronomy, especially good yield productivity is very important in wheat (Triticum aestivum L.) as human food crop. Development of elite cultivar having good traits is required for food stability. In 2013, a new wheat line called Iksan370 has been developed by the National Institute of Crop Science, RDA. Iksan370 has hard red winter wheat characteristics and better features like resistance to biotic, abiotic stress and higher yield ability than Keumkang. Heading and maturing date were delayed about ten days and period to flowering date was shorter three or four days than Keumkang. Culm and spike length of Iksan370 were 95cm and 15.3cm, averagely. This means that culm and spike length were longer than Keumkang (79cm and 7cm, respectively). It showed higher milling rate (75.5%), protein content (13.5%) and SDS-sedimentation volume (77.5ml) than Keumkang. Genotypes of six genes, GBSSA, B, D, PPO18, Pina and Pinb, were represented that were similar to Keumkang, respectively. Average yield of Iksan370 in the preliminary trial test was 6.61 tons/ha in upland. We expect that yield productivity and farm income were increased by Iksan370.

Fusarium head blight (FHB), primarily caused by Fusarium graminearum is a major disease problem on wheat and barley around the world. The objectives of this study were to evaluate for FHB resistance under greenhouse and filed condition in 2012 and 2013. We screened for Type I resistance in the field using spray inoculation and for Type II resistance in the greenhouse using single floret inoculation. Sumai 3 and Ning7840 were used the FHB resistant check. Five hundred and twelve lines were evaluated for resistance to initial infection (type I) and to spread of symptoms within spike (type II). The inoculum was F. graminearum (GZ3639) prepared at a concentration of 1 X 105 macroconidai/ml with Tween 20. The field screening plots were inoculated twice and mist-irrigation was applied to facilitate FHB development. FHB severity was assessed visually 21 days and determined as the percentage of symptomatic spikelets from the total of all spikelets observed in 20 spikes. Both in the greenhouse and field test, we could observe five categories of FHB severity: resistant (R: 0-20%), moderately resistant (MR: 21-40%), moderate (M: 41-60%), moderately susceptible (MS: 61-80%), and susceptible (S: 81-100%). The results showed that seventy seven lines showed the R and MR category on FHB severity. In addition, nine lines showed similar FHB severity compared to Sumai 3 (13.3%).

본 연구는 국내 밀 품종의 국수 색깔 및 이와 관련된 종실 및 밀가루 특성 평가를 통하여 국수 면대 색깔 향상을 위해 선발에 이용할 수 있는 방법을 개발하고, 특히 철함량이 국수면대 밝기에 미치는 영향을 평가하여 영양학적으로 우수한 철 고함유 계통 선발을 위한 간이검정법의 적용을 위해 수행하였다. 국내 밀 품종의 밀가루 밝기는 회분(r =-0.634, P<0.01), 단백질 함량(r = -0.635, P<0.01)및 총 폴리페놀함량(r = -0.493, P<0.05)과 부의 상관을 나타내었다. 면대 색깔은 밀가루 명도(r = 0.684, P<0.001)와 정의 상관을 보였고, 종실특성에서 리터중, 천립중, PPO 활성 및 총 폴리페놀 함량과는 부의 상관을 보였다. 또한 밀가루 특성에서 회분, 단백질 함량과 총 폴리페놀함량은 면대 밝기와 부의 상관을 보였고, 연차간 및 품종간 변이에 영향을 받는것으로 나타났다. 철은 밀가루 색 및 국수 면대 밝기와 부의상관을 나타냈고, 간이검정법으로 Potassium hexacyanoferrate(II) trihydrate(PHT) 처리를 한 결과 푸른색으로 염색되었다. 종실을 PHT로 염색하여 측정한 색도와 종실의 철함량은 상관을 보이지 않았으나, 종실을 분쇄한 통밀가루에서는 PHT로 염색된 통밀가루의 적색도(r = -0.665, P<0.001)와 황색도(r = -0.658, P<0.001)와 부의 상관을 나타내었다. 또한 PHT로 염색된 밀가루의 밝기는 종실의 철함량(r = -0.640, P<0.01)과 부의 상관을 나타내었다.

본 연구에서는 밥밑용 잡곡으로 이용하기 위한 찰옥수수 를 도정하여 이화학특성 및 취반특성에 관련된 연구를 수행 하여 얻어진 결과를 보고하고자 한다. 1. 도정수율은 유색 찰옥수수인 흑진주찰, 미흑찰, 얼룩 찰이 다른 품종에 비해 상대적으로 높았으며, 백색 찰 옥수수 품종에서는 찰옥4호, 일미찰, 연농1호가 높았다. 2. 도정 전 후의 색차변화를 측정하였을 때 도정 후 모든 품종의 L 값은 증가하고 a값은 백색 찰옥수수 품종에 서는 감소하지만, 유색 찰옥수수 품종에서는 전체적으 로 증가하는 경향을 보였으며, 흑진주찰은 도정 후 L, a값이 가장 낮았다. 3. 옥수수쌀의 안토시아닌 색소함량을 측정하였을 때 흑진주찰은 154.1 μg/g, 얼룩찰1호는 42.5 μg/g 함유되 어 있었다. 4. 옥수수쌀 전분의 호화양상을 측정하였을 때 연농1호, 미흑찰은 호화개시온도가 가장 낮았으며, 연농1호, 흑 진주찰, 미흑찰은 최종점도와 치반점도가 낮고, 상대 적으로 강하점도가 높아 호화특성이 양호한 것으로 나 타났다. 5. 취반 후 연농2호, 미흑찰, 흑진주찰이 다른 품종에 비 해 수분흡수율, 퍼짐성이 높고, 연농2호, 연농1호, 미 백2호, 흑진주찰, 얼룩찰이 다른 품종에 비해 경도값 이 낮았다. 6. 취반 후 식미평가에서 흑진주찰 > 미흑찰 > 흑점2호 순으로 높은 기호성을 보였다. 7. 종합적인 결과를 바탕으로 흑진주찰, 연농2호, 미흑찰 품종이 취반용 옥수수쌀로 알맞은 특성을 가지는 것으 로 나타났다.

Korean peanut yield by new varieties has marvelously increased since first variety with 1.09 MT/ha was developed in 1960. This means 77.58kg/ha yield increment every year from 1960 to 2012. A new peanut variety “Sinpalkwang”(Arachis hypogaea ssp. hypogaea L.) showed the highest grain yield, 5.40 MT/ha, of Korean varieties was developed at the Department of Functional Crop, NICS, in Milyang in 2012. This was developed from the cross between cultivar “Palkwang“ and crossing line from cultivar “Palkwang” and “PI156649”. “Sinpalkwang” which is Virginia plant type has 41cm of main stem length and 25 branch number per plant. 45 pod number per plant, 79% of shelling ratio and 92g of 100-grain weight in the regional yield trials(RYT) greatly contributed to increase the yield potential of this variety. Each pod has two grains with brown testa and ellipse shape. Seed quality showed 45.4% of crude oil and 29.2% of protein content. This variety also showed more resistant to web blotch and stem rot, compared with check variety, Daekwang. In the regional yield trials “Sinpalkwang” outyielded check variety by 28% with 5.40 MT/ha for kernel.

Recently, fresh peanut eaten after boil is popular to korean consumer owing to its sweet taste. A new vegetable peanut variety “Ami”(Arachis hypogaea ssp. fastigiata.) was developed at the Department of Functional Crop, NICS, in Milyang in 2012. It was developed from the cross between the short stem cultivar “Satonoka” and the large grain cultivar “Milyang 16”. “Ami” which is Shipung plant type has 50cm main stem length and 10 branch number per plant. Each pod has two grains with brown testa and long-ellipse shape and dried 100-seed weight was 84g in the regional yield trials(RYT). The Sucrose and tannin content of fresh peanut are 24.9mg/g and 4.9mg/g, respectively. Functional compound content was 3944㎍/g of Luteolin in peanut shell, This variety also showed more resistant to stem rot and lodging, compared with check variety, Palkwang. In the regional yield trials “Ami” was outyielded than check variety by 9% with 10.10MT/ha for fresh pod and by 14% with 4.51MT/ha for dried kernel.

콩 관련 제품을 생산하는 과정에서 발생되는 부산물은 가축의 사료 및 퇴비 등에 이용되고 있으나 일부는 폐기물로처리되어 추가 비용 및 각종 환경오염을 유발하는 등 사회적인 문제점으로 지적되고 있다. 따라서 본 연구에서는 가공 부산물로 다량 발생되는 콩 배아를 활용하여 생리활성물질인 isoflavone과 soyasaponin을 동시에 분리하는 방법을개발하고자 실시하였다.1. 콩 배아 methanol 추출물을 preparative C18 column에 주입하고, 210 nm의 파장에서 0.5% 초산 용액 30%로부터100% acetonitrile까지 분당 15mL의 유속으로 53분간흘려주어 isoflavone과 soyasaponin 분획을 동시에 분리하였다.2. Preparative C18 column으로 분리된 isoflavone 및 soyasaponin분획은 동결건조시켜 isoflavone 분말 ISF-1과 soyasaponinSAP-1, SAP-2, SAP-3 및 SAP-4의 분말을 얻었다.3. Isoflavone 분획 ISF-1의 재분리는 젤투과 컬럼에서100% acetonitrile을 분당 5 mL가 되도록 흘려주면서254nm 파장에서 관찰하여 2종의 분획 ISF-1-1 및ISF-1-2을 분리하였다.4. 분리된 2종의 isoflavone 중 ISF-1-1은 그 조성이daidzin, glycitin 및 genistin 이고, ISF-1-2는 genistin 단일물질이 주성분인 것으로 나타났다.5. 분리된 4종의 soyasaponin 중 SP-1은 soyasaponin A계열인 Aa(MW: 1364), Ab(MW: 1436), Ac(MW: 1420),Ae(MW: 1202), Af(MW: 1274), SAP-2는 B계열인 Ba(MW: 958), Bb(MW: 942), Bc(MW: 912)와 E계열인Bd(MW: 956), Be(MW: 940), SAP-3는 B계열인 Ba,Bb, Bc, E계열인 Bd, Be와 DDMP계열인 βg(MW:1068), SAP-4는 B계열인 Ba, Bb, Bc, E계열인 Bd, Be와 DDMP계열인 βg, βa(MW: 1038)가 주성분임을 알 수 있었다.