A pregnancy diagnosis is an important standard for control of livestock’s reproduction in paricular dairy cattle. High reproductive performance in dairy animals is a essential condition to realize of high life-time production. Pregnancy diagnosis is crucial to shortening the calving interval by enabling the farmer to identify open animals so as to treat or re-breed them at the earliest opportunity. MicroRNAs are short RNA molecules which are critically involved in regulating gene expression during both health and disease. This study is sought to establish the feasible of circulating miRNAs as biomarkers of early pregnancy in cattle. We applied Illumina small-RNA sequencing to profile miRNAs in plasma samples collected from 12 non-pregnant cows (“open” cows: samples were collected before insemination (non-pregnant state) and after pregnancy check at the indicated time points) on weeks 0, 4, 8, 12 and 16. Using small RNA sequencing we identified a total of 115 miRNAs that were differentially expressed weeks 16 relative to non-pregnancy (“open” cows). Weeks 8, 12 and 16 of pregnancy commonly showed a distinct increase in circulating levels of miR-221 and miR-320a. Through genome-wide analyses we have successfully profiled plasma miRNA populations associated with pregnancy in cattle. Their application in the field of reproductive biology has opened up opportunities for research communities to look for pregnancy biomarker molecules in dairy cattle.

본 연구는 경남지역 내 주요공원(4곳)에 생존하고 있는 조경 수목의 수목활력도를 파악하여 수목보호 관리에 기초자료를 제공할 목적으로 실시하였다. 부산시민공원 내 관목류의 수목활력도는 불량, 활엽 교목, 침엽 교목은 모두 양호한 수준이었다. 다대포해변공원 내 관목류, 활엽 교목, 침엽 교목은 모두 양호한 수준이었다. 해운대나루공원 내 관목류는 양호한 수준이었으며, 활엽 교목, 침엽 교목은 매우 양호한 수준이었다. 밀양아리랑공원 내 관목류는 양호한 수준이었고, 활엽수 교목과 침엽수 교목은 매우 양호한 수준이었다. 수목별 수목활력도는 침엽수 교목이 가장 높았으며, 그다음으로 활엽수 교목, 관목류 순이었다. 4개의 공원에서 수목의 공원별 수목활력도의 평균이 가장 높은 순서는 해운대나루공원, 밀양아리랑공원, 다대포해변공원, 부산시민공원 순이었다. 계절별 수목활력도 변화는 계절별로 측정된 수목의 수목활력도에서 수목활력도가 가장 높은 순서는 밀양아리랑공원, 해운대나루공원, 부산시민공원과 다대포나루공원 순이었다. 계절별로 변화는 수목의 생장이 활기차게 시작되기 전 3월과 4월의 초봄이 가장 낮았고, 다음으로 가을철 9, 10, 11월이 낮았다. 여름철인 6, 7, 8월이 수목활력도가 높았는데, 가장 높은 시기는 8월이었다.

In this study, the effects of heat-moisture treatment (boiling or steaming for 45 min) prior to hot air drying (70 o C for 8 h) on water activity (Aw), moisture (MC), Brix, color, and texture of dried sweet potato slices were investigated to identify the best pretreatment condition for producing dried sweet potato with the best eating quality. Dried sweet potato slices pretreated by boiling (BL) showed significantly lower Aw, MC, and hardness while Brix was significantly higher than with steaming (ST) treatment. There were no significant differences for the color, springiness, cohesiveness, gumminess, and brittleness indexes. At 8 h drying, the Aw, MC, Brix, and hardness of the BL and ST samples were 0.81 and 0.82, 19.71% and 25.53%, 53.80% and 49.40%, and 20.49 kg/cm 2 and 31.98 kg/ cm 2 , respectively. This information will be useful for manufacturers in the production of dried sweet potato slices. These findings provide evidence of the feasibility of heat-moisture treatments in the production of dried sweet potato.

Particulate matter (PM) has recently been considered one of the most harmful air pollutants to public health. Plants have been known to degrade and deposit particle pollutants with epicuticular wax (EW), and this capacity can be influenced by environmental conditions including relative humidity (RH). The present study examined the effects of RH on EW generation and PM deposition upon leaf surfaces within Asplenium nidus ‘Avis’. The plants were treated in growth chambers with two levels of RH (low: 30% - 40% and high: 80% - 90%) for a period of four weeks, and subsequently exposed to a 30 μg･m-3 concentration of TiO2 particles as a PM resource for 72 hours. The EW ultrastructure on the leaf surface was observed as the thin films type, which was not morphologically changed in the condition of low or high RH treatment. For four weeks of RH treatment, the fresh weight and leaf area per plant were not significant between low and high RH treatment, while dry weight was significantly higher in the high RH condition. We also found that greater amounts of EW per fresh weight, dry weight and leaf area were generated in high RH. However, the total amounts of PM deposition (surface PM + in-wax PM) of the plants were higher within the low RH treatment with a higher proportion of surface PM. In contrast the proportion of in-wax PM was 15% higher within the high RH. These results suggest that EW generation is affected by air humidity and that proportion of PM deposition in the EW layer were influenced by the amount of total wax load.

본 연구는 제비동자꽃의 대량번식을 위한 효과적인 발아 조건을 선발하고자 수행되었다. 제비동자꽃 종자는 저온습윤 처리(5°C, 0, 4, 8, 12, 16주), GA3 처리(0, 500, 1000mg･L-1), 프라이밍 처리(0.1M, 0.2M의 NH4NO3, K2HPO4, KCl, KNO3)를 하였다. 모든 종자는 25°C, 12시간 광조건(40μmol･m-2 ･s-1)에 서 발아 실험이 진행되었으며, GA3 처리구는 비닐온실(평균 23.4°C)에서도 비교실험이 진행되었다. 저온습윤 8주 처리구 에서 20.4%로 가장 높은 발아율을 나타냈으며, 12주 이상의 저온습윤 처리는 오히려 제비동자꽃의 발아율을 감소시켰다. GA3 0, 500, 1000mg･L-1 처리된 종자는 25°C에서 각각 3.3, 44.0, 62.0%, 비닐온실에서 각각 5.3, 16.7, 42% 발아하였다. 제비동자꽃 종자는 GA3 농도가 높아질수록 발아율이 증가하였다. 또한 제비동자꽃 종자는 0.2M KNO3-프라이밍 처리구에서 가장 높은 발아율(54.7%)을 나타냈으며, 평균발아소요일수는 처리 간 유의성을 보이지 않았다. 이상의 결과로 효과적인 제비종자꽃 종자 번식을 위한 전처리 조건으로 24시간 GA3 1000mg･L-1 침지 처리 또는 0.2M-KNO3 프라이밍 처리를 추천한다.

This article reports on a classroom-based study that examined the effects of direct focused and unfocused written corrective feedback (WCF) on the accuracy development of the past hypothetical conditional (e.g., If I had had money, I would have bought a new computer.) and the indefinite article (a/an). The study employed a pretest-posttest-delayed posttest design with two feedback treatment sessions. Six intermediate classes, a total of 108 college freshmen at a university in Korea and two native English speaking instructors, participated in the study. The classes were assigned into a focused WCF group, an unfocused WCF group, and a control group. A text-reconstruction task was used as a writing material and a test instrument. The results revealed that both focused and unfocused WCF groups outperformed the control group in the accuracy gains of both target forms. When the relative effects of two types of feedback were considered, there was no difference in the contribution focused and unfocused WCF made for the accuracy improvement of the hypothetical conditional. However, focused WCF was more beneficial than unfocused WCF for the development of the indefinite article.

In this paper, nitrogen-doped reduced graphene oxide(rGO) is obtained by thermal annealing of nitrogen-containing compounds and graphene oxide (GO) manufactured by modified Hummers' method. We use melamine as a nitrogen-containing compound and treat GO thermally with melamine at over 800 ~ 1,000℃ and 1 ~ 3 hr under Ar atmosphere. The electrical conductivity of doped rGO is measured by 4-point probe method. As a result, nitrogen contents on rGO are found to be in the range of 2.5 to 12.5 at% depending on the doping conditions after thermal annealing. The main doping site on graphene oxide is changed from pyridinic-N and pyrrolinic N to the graphitic site as the heat treatment temperature increases. The electrical conductivity of doped rGO increases as the N doping content increases. As the thermal treatment time increases, the change of both total doping contents and doping sites is slight and the surface resistance is remarkably reduced, which is caused by healing effects of doped graphene oxide at high temperature.

We conducted a survey of open clusters within 1 kpc from the Sun using the astrometric and photometric data of the Gaia Data Release 2. We found 655 cluster candidates by visual inspection of the stellar distributions in proper motion space and spatial distributions in l 􀀀 b space. All of the 655 cluster candidates have a well dened main-sequence except for two candidates if we consider that the main sequence of very young clusters is somewhat broad due to dierential extinction. Cross-matching of our 653 open clusters with known open clusters in various catalogs resulted in 207 new open clusters. We present the physical properties of the newly discovered open clusters. The majority of the newly discovered open clusters are of young to intermediate age and have less than 50 member stars.

본 연구는 새만금 간척지에서 방풍시설의 설치에 따른 수림대의 초기 생존율을 분석하기 위하여 생존 분석 기법 중의 하나인 생명표법을 적용하였다. 생명표법은 의학, 사회과학 등 많은 분야에서 활용하고 있는 방법 이며, 본 연구에서는 방풍책의 설치에 따른 초기 수림대 수목의 생존 기간별 생존율, 중위 시간 등을 생명표 를 통해 알아보고, 생존함수 그래프와 위험함수 그래프 그리고 수림대별 비교를 통해 생존율의 차이를 분석 하고자 하였다. 주요 결과로는 곰솔 수림대의 중위 시간은 100% P-tb와 25% P-tb에서는 48.00개월 이었 고, 50% P-tb는 20.85개월로 짧았다. 25% P-tb에서 누적 생존율이 가장 높았으며, 위험률은 50% P-tb가 가장 높았다. 졸참나무 수림대의 경우 모든 수림대에서 중위 시간은 48.00개월 이었으며, 50% P-tb에서 누 적 생존율이 가장 높았고, 위험률은 100% P-tb에서 가장 높았다. 생존율 비교 결과, 곰솔 수림대는 전체적 으로 방풍책의 설치가 생존율에 영향을 미쳤으며, 특히 50% P-tb와 25% P-tb에서 유의미한 차이가 나타났 는데, 25% P-tb가 생존율과 위험률 측면에서 우수한 결과를 나타내었다. 반면에 졸참나무 수림대의 경우 전 체 수림대의 생존율이나 수림대별 생존율은 방풍책의 설치에 영향을 받지 않는 것으로 분석되었다.