충북의 한지형 마늘 재배지에서 파총채벌레와 파좀나방의 발생양상과 약제검정을 수행하였다. 파총채벌레 발생양상은 3월 중순에 발생하기 시작하여 5월 중하순경 부터 밀도가 급격히 증가하였다. 파좀나방은 3월 중하순에 발생이 시작되어 수확기인 6월 중순까지 2화기 발생하였다. 9종의 살충제를 이용하여 파총채벌레와 파좀나방의 약제감수성을 조사하였다. 실내 검정 결과 파총채벌레에 대하여 clothianidin SC, chlorfenapyr EC, dimethoate EC, imidacloprid WP, cyantraniliprole + thiamethoxam WG가 100%의 살충효과를 보였다. 파좀나방에 대해서는 cyantraniliprole EC, pyridalyl EW, spinetoram SG, abamectin + chlorantraniliprole SC가 100%의 살충효과를 보였다. 2종 해충에 대하여 실내검정에서 살충효과가 높았던 약제 각각 5, 4종을 대상으로 포장검정을 수행한 결과 모든 약제에서 처리 3일차에서 90% 이상의 방제효과를 나타내었다.

Urbanization is one of the leading causes of habitat loss, habitat degradation, and fragmentation. Urban development negatively affects biodiversity. This study aimed to clarify the change of butterfly communities on effect of urbanization in urban green areas. Butterfly survey was conducted using the line transect methods from April to October in 2012. A total of 59 species and 1,465 individuals of butterflies were observed in four urban green areas: Namsan Park (NS), Ewha Womans University (EW), Bukseoul Dream Forest (BD), and Hongneung Forest (HF), and natural forest: Gwangneung Forest (GF). The category of land use around study site was determined based on GIS data. Species richness and abundance of niche breadth and habitat type in urban green areas differed significantly from those in GF. Estimated species richness and species diversity (H’) in four urban green areas were significantly lower than those in GF. Species richness and abundance of forest interior species and specialist were positively correlated with paddy, field, and forest, whereas those of forest interior species and specialist were negatively correlated with urban area and road. Butterfly communities in four urban green area differed from that in GF. The result suggests that the decrease of paddy, field, and forest associated with increase of urban area and road negatively influences species composition and changes butterfly communities.

To profile the proteome in porcine plasma, blood samples were collected from adult male barrows and those plasma were retrieved. For the depletion or pre-fractionation of high-abundance proteins, plasma samples were treated with commercial kits. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than forty six proteins were identified and the reference map was constructed. The pre-treatment for the removal of high-abundance proteins caused the changes in 2-DE images and some of the proteins were newly uncovered after the most of high abundant proteins were removed. However, it is expected for further steps necessary to identify more low-abundance proteins that may contain potential bio-markers.

Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-di-mensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the re-sults, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young pig-let separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was car-ried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age de-pendent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.

지구 온난화에 따른 한지형마늘의 적정 파종시기를 구명하고자 10월 20일부터 10일 간격으로 4회 파종하여 시험하였다.한지형마늘 파종시기에 따른 출현율은 85.6~90.3%로 차이가없었으며, 지상부 생육은 초장, 엽폭, 엽수 등 지상부 생육은파종시기 간 차이가 없었다. 지하부 특성은 주당 구중은 25.1~28.8g, 구경은 40.1~42.1mm, 구고는 33.6~34.7mm, 구당쪽수는 6.1~6.3개로 파종시기에 따른 차이가 없었다. 2차생장비율은 파종시기 간 차이가 없는 47.8~56.7%로 높은 경향이었으며, 총포의 무게와 총포경은 파종시기 간 유의성은 인정되지 않았다. 총수량과 상품수량을 고려한 한지형마늘의 파종한계기는 11월 10일이었다.

2008년부터 2010년까지 3년간 인삼재배 29농가를 대상으로 묘삼 이식기종별 경제성을 분석, 손익분기 규모를 추정하여 인삼재배농가의 생력화를 위한 기초 자료로 제공하고자 연구한 결과를 요약하면 다음과 같다. 1. 묘삼이식기종에 따른 이식작업의 생력화율은 관행이식 대비 모종이식기 16%, 기계이식기은 49%, 기계이식기는 63%정도의 노력이 절감되었다. 2. 묘삼 이식기에 따른 손익분기 규모를 분석한 결과, 관행이식 대비 기계이식기은 보조지원을 받지 않는 경우 0.9 ha, 기계이식기은 1.3 ha 정도에서 손익분기점이 나타났다.

에멀션 기반의 계면활성제를 이용한 주형합성법을 이용하여 산촉매로서 염산과 실리카의 전구체인 테 트라에톡시실란을 사용함으로써 메조다공성 실리카 마이크로스피어를 합성하였다. 테트라에톡시실란의 농도 증가에 의해 구형의 입자 형태가 파괴되었고, 기공구조도 크게 변하였다. 산촉매 농도 증가에 의한 구형의 입자형태 파괴 현상은 적었지만 상대적으로 작은 크기의 구형의 마이크로입자가 더 많이 생성되 었다. 하지만, 산성조건에서 입자들 간의 강한 응집현상이 나타남에 따라 낱개의 분리되어 있는 단일입자 를 얻기 위해서는 초음파 등의 후처리 과정이 필요하였다.

This study was performed to develop the mass propagation system using tissue culture technique to supply the seeds of Elephant garlic (Allium ampeloprasum L.) which has difficulty in propagation. Immature spathe of Elephant garlic was cultured on Murashige & Skoog (MS) medium supplemented with two plant growth regulators, naphthaleneacetic acid (NAA) and kinetin. After 6 weeks of culture, the highest number of shoot (14.9/explant) was obtained when the immature spathe with 10 ㎝ length was cultured right after harvesting. In MS medium supplemented with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the most vigorous growth characteristics was observed, the shoot number was 14.9/explant, its length was 11.3 ㎝, and its fresh weight was 2.5 g. When the bulblets were cultured in MS medium with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the addition of 30 ㎎/L adenine improved their proliferation and growth significantly, the highest bulblet formation rate (48%) was obtained. The addition of 7% sucrose also increased the bulblet formation rate at the highest frequency of 98.2%. The shoots were shown be more vigorously proliferated at the secondary subculture stage rather than primary culture stage, their propagation rate was 80% after subculture.

The seasonal variation of phytoplankton communities and its standing crops in accordance with environmental factors were investigated at Yeongrang-ho, Cheongcho-ho, Mae-ho and Hyang-ho which are located in the East coast of Korea during April to October 2011. High concentrations of T-N and T-P were observed in four lagoons due to the inflowing nutrients from farmlands and residential areas near lagoons. Nutrient salts mainly composed of nitrogen and phosphate caused the eutrophication of the lakes, leading to the robust growth of phytoplankton. During the period of investigation, 64 to 107 taxa of phytoplankton were observed in four lagoons. Diatoms were the most dominant species with high density. Non-point pollution source was estimated as the main cause of water pollution of lagoons. Trophic states of lagoons evaluated using the value of TSI and TSIKO indicated that all the investigated lagoons were in the eutrophication state.

충북농업기술원 마늘연구소에서는 2000년부터 수집한 유전자원 중 한지형인 ‘단양종’ 보다 조숙다수성이며 불완전추대의 특성을 가진 계통을 5년 동안 지속 선발하여 ‘단산’ 품종을 육성하였다. ‘단산’ 마늘의 추대 특성은 불완전 추대형으로 ‘단양종’의 완전 추대형과 뚜렷한 구별성을 갖는다. 따라서 화경이 출현하지 않아 수확 전에 쫑을 뽑을 필요가 없으며 숙기도 6일 정도 빨라 수확기 때 장마를 피할 수 있는 장점을 갖고 있다. 2차 생장비율은 3.3%로 ‘단양종’이 17.3% 보다 매우 낮아 이차생장율이 적은 상품성 마늘을 생산할 수 있다. ‘단산’의 통의 크기는 ‘단양종’ 보다 크고, 쪽 껍질의 색은 갈색을 띠며 안토시아닌 줄무늬가 존재한다. 수량은 1,131 kg/10 a로 ‘단양종’ 보다 18% 정도 높다.