본 연구는 제비동자꽃의 대량번식을 위한 효과적인 발아 조건을 선발하고자 수행되었다. 제비동자꽃 종자는 저온습윤 처리(5°C, 0, 4, 8, 12, 16주), GA3 처리(0, 500, 1000mg･L-1), 프라이밍 처리(0.1M, 0.2M의 NH4NO3, K2HPO4, KCl, KNO3)를 하였다. 모든 종자는 25°C, 12시간 광조건(40μmol･m-2 ･s-1)에 서 발아 실험이 진행되었으며, GA3 처리구는 비닐온실(평균 23.4°C)에서도 비교실험이 진행되었다. 저온습윤 8주 처리구 에서 20.4%로 가장 높은 발아율을 나타냈으며, 12주 이상의 저온습윤 처리는 오히려 제비동자꽃의 발아율을 감소시켰다. GA3 0, 500, 1000mg･L-1 처리된 종자는 25°C에서 각각 3.3, 44.0, 62.0%, 비닐온실에서 각각 5.3, 16.7, 42% 발아하였다. 제비동자꽃 종자는 GA3 농도가 높아질수록 발아율이 증가하였다. 또한 제비동자꽃 종자는 0.2M KNO3-프라이밍 처리구에서 가장 높은 발아율(54.7%)을 나타냈으며, 평균발아소요일수는 처리 간 유의성을 보이지 않았다. 이상의 결과로 효과적인 제비종자꽃 종자 번식을 위한 전처리 조건으로 24시간 GA3 1000mg･L-1 침지 처리 또는 0.2M-KNO3 프라이밍 처리를 추천한다.

This article reports on a classroom-based study that examined the effects of direct focused and unfocused written corrective feedback (WCF) on the accuracy development of the past hypothetical conditional (e.g., If I had had money, I would have bought a new computer.) and the indefinite article (a/an). The study employed a pretest-posttest-delayed posttest design with two feedback treatment sessions. Six intermediate classes, a total of 108 college freshmen at a university in Korea and two native English speaking instructors, participated in the study. The classes were assigned into a focused WCF group, an unfocused WCF group, and a control group. A text-reconstruction task was used as a writing material and a test instrument. The results revealed that both focused and unfocused WCF groups outperformed the control group in the accuracy gains of both target forms. When the relative effects of two types of feedback were considered, there was no difference in the contribution focused and unfocused WCF made for the accuracy improvement of the hypothetical conditional. However, focused WCF was more beneficial than unfocused WCF for the development of the indefinite article.

83세 여자 환자가 신장결석으로 입원하여 금식, 세프트리악손 투여 및 경피적 신쇄석술 후 퇴원하였고, 이후 명치 통증이 발생하여 응급실로 내원하였다. 혈액검사 및 복부 전산화 단층촬영에서 담석 및 췌장염 소견을 확인하였고, 담석은 이전에 보이지 않던 소견이었다. 이에 세프트리악손 유발 담석에 의한 급성 췌장염으로 진단하였고 보존적 치료 및 담낭절제술 후 퇴원한 예를 경험하여 보고하는 바이다.

In this study, commercial activated carbons (ACs) were upgraded by different activation methods, and the gases generated during the activations were defined and quantified. The chemical activation commonly applied for upgrading ACs uses complex reactions, involving pyrolysis, physical, and chemical reactions. The ACs based on wood materials were characterized by elemental analysis, N2 physisorption, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and temperature-programmed desorption mass spectrometry. The patterns and composition of the generated gases were analyzed by gas chromatography and X-ray diffraction; high-resolution scanning electron microscopy was also used to characterize the activated carbon. The AC was mostly decomposed to CO2 by pyrolysis and physical activation, while CO was mainly detected during chemical activation from the K2CO3 produced by the reactions between CO2 and K2O. The detected amounts of generated gases were differed at various KOH ratios and residence times. The highest surface area obtained in this study was 2000 m2/g at the optimum ratio of AC and KOH (1:2).

This study investigated the effects of negotiation in the process of providing written corrective feedback (WCF) by comparing three different patterns: non-negotiation, minimal negotiation, and extended negotiation. The data were collected from three intermediate academic English classes at a university in Korea. Each class was exposed to different degrees of negotiation while they received WCF for two target forms, the past verb forms and the English articles, in their written production (text re-construction tasks). Their accuracy improvement was measured by text-reconstruction writing tasks and error correction tests. The study found that the use of negotiation affected accuracy improvement of the target forms; however, its effectiveness varied depending on the target forms and measurements. For the past tense, negotiation, regardless of its amount, resulted in more accuracy improvement than non-negotiation in both text re-construction tasks and error correction tests. However, for the English articles, negotiation operated differently: extended negotiation significantly brought about more accuracy development in both measurements, but the difference between minimal negotiation and non-negotiation was only observed in the error correction test, not in the text re-construction task.

목적: 이 연구는 안경과 선글라스에서 테와 렌즈의 형태와 색상에 대한 한국인과 중국인 소비자 집단의 선호도 차이를 조사하기 위하여 실시되었다. 방법: 한국에 유학 중인 중국인 학생 150명과 한국인 대학생 150명을 대상으로 설문조사를 실시하고 그 결과를 분석하였다. 안경광학 관련 전공자는 설문 대상에서 배제되었다. 중국인 학생들에게는 중국어 간체자로 번역된 설문지를 제공하였으며, 제공된 설문지는 한국 대학 석사과정에 유학 중인 중국인 원어민 학생이 한국어 설문지를 바탕으로 번역한 것이다. 결과: 중국인 소비자 집단은 안경보다 선글라스에서 글로벌 유명 브랜드를 더 선호하였으며, 한국산 안경 제품을 상대적으로 선호하는 것으로 나타났다. 또한, 무테 또는 반무테, 금색테, 투톤컬러 렌즈 항목에서 한국인 소비자 집단보다 높은 선호도를 나타냈다. 결론: 이 연구의 결과는 한국 안경 제조․유통 업체의 중국 시장 진출이나 중국인 고객 비율이 높은 안경원 의 판매 전략 수립에 기여할 수 있을 것으로 기대된다.

The study investigated the effects of recasts and prompts on learning language forms that arose incidentally in dyadic interaction, focusing on the degree of explicitness of each type of feedback and learner proficiency levels. The data were collected from 64 beginning and upper-intermediate English learners of Korean. Thirty-one beginning learners were randomly assigned to a feedback group and to a control group, and thirtythree upper-intermediate learners were also assigned to a feedback group and to a control group. Each learner was paired with an English speaker and worked on a picture-sequencing task. The learners in the feedback groups received recasts or prompts on their erroneous utterances while the learners in the control groups did not. The effects of feedback were measured by pre-interaction picture descriptions and immediate and delayed post-interaction correction tasks. The study found that both recasts and prompts had some effects on learning the targeted forms, and more explicit forms of each feedback resulted in a higher rate of correction. The beginning learners took more advantages of recasts relative to the upper-intermediate learners, and explicit prompts worked better for the upper-intermediate learners. The effects of prompts sustained longer than recasts in both levels.

Cryopreservation of bovine embryos is used to efficiently implant surrogate mothers. It has been widely accepted that high lipid content in the oocyte interrupts its survival during freeze-thaw cycles. Serum component in the culture medium is thought to increase the embryo`s lipid contents. Conversely, L-carnitine stimulates lipid metabolism by transporting long chain fatty acids into the mitochondria. Objective of this study was to analyze the effect of L-carnitine supplementation in IVM medium and defined IVC medium on the development, lipid contents and the cryosurvival of bovine IVF embryos. 0.0, 1.5, 3.0 and 6.0 mM L-carnitine was supplemented in IVM medium, respectively (IVM-LC 0.0, LC 1.5, LC 3.0 and LC 6.0). Development rate from the 2cell to the morula stages was higher in IVM-LC 3.0 groups than those of IVM-LC 6.0 (p<0.05). But there were no significant differences among the other groups in the blastocyst rates and lipid content results. When 0.0, 1.5, 3.0 and 6.0 mM L-carnitine were supplemented in IVC medium (IVC-LC 0.0, LC 1.5, LC 3.0 and LC 6.0), development competence was not significantly different between those embryos. Lipid contents of embryos treated L-carnitine (IVC-LC 1.5, 3.0 and 6.0) were significantly lower than embryos of non-treated group. L-carnitine was supplemented 0.0, 1.5, 3.0, 6.0 mM during IVM and 3.0 mM during IVC (LC 0.0 - 3.0, LC 1.5 – 3.0, LC 3.0 – 3.0, LC 6.0 – 3.0) and cryosurvival of blastocysts confirmed after freezing-thawing. There were no significant differences on development, but LC 3.0 – 3.0 was significantly lower lipid contents than other groups. And LC 3.0 – 3.0 had better survival rates and hatched rates of blastocysts than LC 0.0 – 0.0. In conclusion, supplementation of L-carnitine in defined IVC medium decreases lipid contents. And L-carnitine supplementation improves cryosurvival and developmental ability of bovine IVF embryos.

The early-onset familial Alzheimer's disease (EOFAD/ FAD), the less common type of Alzheimer's disease (AD) currently affects a vast number of individuals worldwide. This type is being inherited as an autosomal dominant fashion. Missense mutations on Amyloid precursor protein (APP) and Presenilins 1 and 2 (PSEN1 & PSEN2) are known as major genetic factors in FAD. Conversely, missense mutations on microtubule-associated protein tau (MAPT) are also thought to involve. Up to date, several triple-transgenic animal models with muted forms of the human APP, PSENs and MAPT have been reported. Compared to other animals, canines are more emotional and their disease signs can be easily diagnosed. This attempt was to develop a triple transgenic canine model for the AD. We have obtained the coding sequences of APP, PSEN1 and MAPT from Dana-Farber/Harvard Cancer Center DNA resource core at HMS and incorporated several common AD mutations. The transgenic construct is composed of hNSE (ENO2) promoter-driven three AD genes fused together with modified 2A sequences. It was transfected into the canine fetal fibroblasts which were then used to perform somatic cell nuclear transfer (SCNT). The viable transgenic embryos were obtained after in vitro culture and the GFP was detected. In this study, we have successfully produced viable triple transgenic canine cloned embryos using SCNT technique. These transgenic canine embryos will be further developed into canines with FAD. The transgenic canines will be a good candidate in the AD research field.

본 연구는 부모의 스마트폰 중독이 유아의 놀이성에 미치는 영향에서 유아의 스마트기기 이용수준의 매개효과를 밝히고자 했다. 이를 위해 만 3∼5세 유아와 그들의 어머니 259명을 대상으로 설문조사를 실시하였다. 수집된 자료는 R version 3.5.1과 Mplus Version 7.4 Base Program Single-User을 이용하여 분석했다. 본 연구의 결과는 다음과 같다. 첫째, 부모의 스마트폰 중독은 유아의 놀이성에 부적인 영향을 미쳤으나 유아의 미디어기기 이용수준에는 정적인 영향을 미쳤다. 그리고 유아의 놀이성에 대해 유아의 미디어기기 이용수준은 부적인 영향을 미쳤다. 둘째, 부모의 스마트폰 중독과 유아의 놀이성의 관계에서 유아의 미디어기기 이용수준도 부분 매개역할을 하는 것으로 나타났다. 본 연구결과는 스마트 기기를 사용할 수밖에 없는 현대인의 삶에서 유아의 스마트기기 이용 수준이 유아의 놀이성에 긍정적인 영향을 미치는 주요한 요인임을 시사한다.

본 연구에서는 김치 제조시 철저한 재료 세척의 필요성을 확인하고, 더불어 비가열 식품인 김치의 위생 안전성 실태를 파악하여 효율적 미생물 저감화 방안을 마련하고자 국내에서 유통되는 김치 원·부재료 200건의 세척 전과 후의 비교분석과 시판 김치 100건에 대하여 미생물 분석을 실시하였다. 김치 원·부재료 및 완제품에 대하여 일반 세균수, 대장균 및 대장균군을 모니터링 하였으며, 병원성 미생물 9종(Bacillus cereus, Clostridium perfringens 정성·정 량, Staphylococcus aureus, Enterohemorrhagic Escherichia coli, Campylobacter jejuni/coli, Yersinia enterocolitica, Salmonella spp., Vibrio parahaemolyticus, Listeria monocytogenes 정성)을 분석 하였다. 모니터링 결과, 김치 원· 부재료 및 완제품에서 일반세균은 1.4~9.0 log CFU/g 수준 으로 확인되었으며, 원·부재료 중 생강에서 8.8 log CFU/g, 완제품 중 총각김치에서 9.0 log CFU/g으로 일반세균이 가장 높게 나타났다. 대장균군은 0.5~7.3 log CFU/g으로 확 인되었으며, 생강에서 7.3 log CFU/g으로 가장 높게 나타 났고, 병원성 미생물 9종의 분석결과, Bacillus cereus를 제 외한 나머지 8종의 식중독균은 모든 시료에서 검출되지 않았다. 김치 제조 시 사용되는 김치 원·부재료들의 세척 공정 후 미생물 수는 일반세균수 0.2~3.2 log CFU/g, 대장 균군 0.3~2.7 log CFU/g, Bacillus cereus 1.0~3.9 log CFU/g 감소하였다. 따라서, 김치 원·부재료의 세척 공정으로 미생물 오염도를 감소 시켰으며, 이 결과를 통하여 김치 완 제품의 위생 및 저장성 증진에 기여 할 수 있을 것으로 확인되었다.

풀무치(Locusta migratoria)는 메뚜기과에 속하는 곤충으로 70% 이상의 단백질을 함유하고 있어 식사료용 곤충으로 이용 가능성이 높다. 그러나 풀무치의 대량생산을 위한 연중사육 관련 연구는 미흡하여 사육방법 정립, 먹이원 선발 등이 필요하다. 본 연구에서는 풀무치의 연중사육을 위한 방법으로 사육밀도에 따른 생육 특성과 최적 산란처를 찾기 위해 상토, 오아시스 등을 이용하여 산란 특성을 조사하였다. 풀무치의 생육 온도는 28℃, 습도는 65% 내외로 유지하고 처리구별 생존율, 탈피횟수, 크기, 무게 등을 측정하였다. 사육밀도별 약충 기간은 32일 소요되었고, 생존율은 82~90 %였으며, 성충 기간은 110~120일 소요되었다. 산란처별 난괴 길이는 33~49mm였으며, 부화 후 생존율을 72~82%였다.

The tobacco cutworm, Spodoptera litura(Fabricius) is serious pest that hurts tomato crops, actives long time of the year. Without pesticide applications, sex pheromone delta traps through experiment first and second half setting during cultural seasons in glasshouse and greenhouse. We investigated captured number of S. litura both in and out side of the two type of tomato cultivated greenhouse. As a result of investigation, captured number of S. litura showed the wave and three high peak typed graph throughout 10 months experiment. First time expression of S. litura was late April in outside installed traps and after that occurrence rate increased gradually until late August. In september, the rate decreased remarkably to middle November in both in and out side treated traps. The result also expressed that captured average number of S. litura in inside of the greenhouse were 8~10 units, and were 15~20units in outside treated traps. Simultaneous installation of internal and external sex pheromone traps succeeded in eliminating a considerable number of S. litura male moths, and the number of larvae was expected to be considerably reduced. Consequently in the experiment, captured male number of S. litura were about 220 units in outside, on the other about 80 of S. litura captured in inside installed traps.