This paper addresses a scheduling problem aimed at minimizing makespan in a permutation flow shop with two machines and an inspection process that must be conducted at least once every certain number of outcomes from the first machine. A mathematical programming approach and a genetic algorithm, incorporating Johnson's rule and a specific mutation process, were developed to solve this problem. Johnson's rule was used to generate an initial population, while the mutation process ensured compliance with the inspection constraints. The results showed that within a computation time limit of 300 seconds, the mathematical programming approach often failed to provide optimal or feasible solutions, especially for larger job sets. For instance, when the process times of both machines were similar and the inspection time was longer, the mathematical programming approach failed to solve all 10 experiments with just 15 jobs and only had a 50% success rate for 100 jobs. In contrast, the proposed genetic algorithm solved all instances and delivered equal or superior results compared to the mathematical programming approach.

This study evaluated the immunogenicity of the Bacillus Calmette-Guérin (BCG) vaccine in a guinea pig model to refine preclinical assessment methods. 24 guinea pigs were divided into four groups for immunohistochemical, histopathological, and molecular analyses, including qRT-PCR and ELISA. The ELISA results revealed significant elevations in interleukin 2 (IL-2), interferon-gamma (IFN- ), and tuberculosis-specific antibodies in vaccinated guinea pigs, particularly γ notable after 6 weeks. Although lung cytokine levels remained unchanged, spleen gene expression showed significant differences in interleukin-17, interleukin-12, interleukin-1β, and C-X-C motif chemokine ligand 10 after 6 weeks. Immunohistochemistry revealed peak IL-2 expression at 8 weeks and significant IFN-γ and TNF-α expression at 6 weeks. This study confirmed the effectiveness of BCG vaccine in guinea pigs, providing crucial insights for future tuberculosis vaccine development and standardizing immune response indicators.

This study aimed to determine the changes in the vitamin B5 content of raw and cooked vegetables. The nineteen vegetables were subjected to different cooking methods, viz. blanching, boiling, pan-broiling, and steaming. Vitamin B5 was quantified by reversed-phase high-performance liquid chromatography (HPLC) using photodiode-array (PDA) detection (200 nm). The standard reference materials (SRM) were used to validate the accuracy of vitamin B5 measurement method used in this study. The cooking yields ranged from 82.63 to 107.62% and decreased in most of the vegetables except bitter melon, curled mallow, and eggplant. The raw kabocha squash, Danhobak, had the highest vitamin B5 content (0.671 mg/ 100 g) among the samples. All cooked vegetables showed lower vitamin B5 content compared to the raw samples. The true retention ranged from 0% (crown daisy, blanching) to 84.49% (kabocha squash, steaming). These results indicate that vitamin B5 is degraded after cooking. Pan-broiling and steaming are better cooking methods than the others for retaining vitamin B5. The true retention of vitamin B5 in the samples markedly depends on the cooking method and food matrix. These results can be used as important basic data for nutritional evaluation of meals.

시판 유통 중인 건조 인삼류(백삼 24점, 홍삼 26점) 포 장 제품을 수집하여 곰팡이 발생 조사를 수행하였다. 백 삼과 홍삼은 각각 50%와 46%의 시료에서 곰팡이가 검출 되었고 검출 시료의 평균 곰팡이 오염도는 각각 0.5와 0.2 log10 CFU/g이었다. 백삼에서는 Penicillium polonicum, P. chrysogenum, Rhizopus microsporus가 각각 18.2%로 우점하 였으나 홍삼은 Aspergillus spp.이 87.6%로 우점하였으며 이 중 A. chevalieri가 50.0%로 가장 높았다. 이 중 독성 종으로 알려진 P. polonicum, P. chrysogenum, P. melanoconidium, A. chevalieri 균주의 citrinin 독소 생성 가능성을 분석한 결 과, 13 균주 모두 독소 유전자가 검출되지 않았다. 이 결 과는 조사한 시료의 곰팡이독소 오염 위험은 매우 낮지만, 건조 인삼류에 곰팡이 오염이 가능함을 보여준다.

In this study, the dietary fiber content of 33 kinds of agricultural products and seaweeds was compared with that of raw products after heat treatment. To verify the total dietary fiber analysis method, the recovery rate was reviewed by measuring the total dietary fiber content for 4 standard certified substances. As a result, the recovery rate of the analysis value for the true value was 98.8%~103.1%, which was judged to be reliable. The total dietary fiber of vegetables ranged between 0.61~5.36 g/100 g for raw vegetables and 0.55~4.84 g/100 g for heat-treated vegetables. Among the 24 kinds of vegetables used in the analanalysis, the total dietary fiber content of heat-treated Korean radish (3.13 g/100 g) was the highest compared to that of raw radish (0.61 g/100 g). The total dietary fiber of beans was between 13.86~29.69 g/100 g for raw beans and 6.72~18.40 g/100 g for heat-treated beans. In particular, the total dietary fiber content of sword beans was the highest in both raw (29.69 g/100 g) and boiled (18.40 g/100 g) beans. The total dietary fiber content of the three types of seaweed was 1.93~4.85 g/100 g in raw seaweed and 0.99~5.72 g/100 g in heat-treated seaweed.

This study deals with replacement analysis of deteriorated equipment for improving productivity of production system. Frequent breakdown of the deteriorated equipment causes a situation that reduces productivity such as low product quality, process delay, and repair cost. However, the replacement of new equipment will be required a high initial investment cost, so it is important to analysis the economic feasibility. Therefore, we analyze the effect of the production system due to the aging effect of the equipment and the feasibility of equipment replacement based on the economic analysis. The process flow, working time, logistics movement, etc. are analyzed in order to build the simulation modeling for a ship and land switchboard production system. Using numerical examples, the economic feasibility analysis of equipment replacement through replacement of existing deteriorated equipment and additional arrangement of new facilities is performed.

위치 기반 AR 게임에서 모바일 기기의 위치를 정확하게 추정하는 것은 중요한 요소 중 하나이다. 모바일 기 기에 내장되어 있는 위치 추정 시스템이 얼마나 정확하게 위치정보를 추정하는 지가 증강되는 AR 콘텐츠의 정확도를 결정한다. 하지만 도시 환경에서는 건물, 건축물, 광고판, 표지판 등 지형지물 및 장애물에 의해서 위치 추정을 위해 필요한 신호가 반사, 굴절, 회절, 차단 등이 발생하게 되고 그로 인하여 모바일 기기의 위 치 추정 시스템으로부터 추정되는 위치에 오차가 생기게 된다. 본 논문에서는 도시 환경에서 모바일 기기의 위치 오차가 증가하는 현상에 대해서 상용 스트리트 뷰와 문자 태그를 활용하여 심플하면서도 신뢰성 있는 위치 보정 방안을 제안한다. 제안하는 위치 보정 방안은 쿼리 이미지와 스트리트 뷰 파노라마 이미지로부터 생성된 문자 태그를 대조하는 것을 통해서 매칭 스코어를 계산한고 매칭 스코어에 따라 쿼리 이미지를 촬영 한 실제 위치와 가까운 스트리트 뷰를 검색하는 것을 통해서 모바일 기기의 위치를 보정한다. 제안하는 위치 보정 방안은 위치 오차가 43.71m인 위치를 위치 오차가 4.09m인 위치로 보정하였으며 낮과 밤에 관계없이 위치를 보정할 수 있다는 장점이 있다.

새싹삼의 곰팡이 발생을 조사하기 위해 18점의 유통중 인 새싹삼을 수집하여 곰팡이 발생빈도를 분석하였다. 전체 시료의 총 곰팡이 발생빈도는 평균 113.3-174.1%였고 Penicillium spp.의 발생빈도가 가장 높았다. 곰팡이 발생 빈도는 이끼가 잎, 줄기, 뿌리보다 유의하게 높았다. 잎과 줄기에서는 Penicillium spp.이, 뿌리에서는 Fusarium spp.의 발생이 높았으며 각각의 우점종은 P. olsonii와 F. oxysporum으로 동정되었다. 계통발생학적 분석을 통해 Fusarium spp.은 총 9개 종, Aspergillus spp.은 A. westerdijkiae와 A. favus, Penicillium spp.은 총 11개 종이 동정되었다. 곰팡이독소 생성 종으로 알려진 25균주의 독소형을 PCR로 검정한 결과 19점의 균주에서 각 독소형이 확인되었다. 이 중 A. flavus 2점과 A. westerdijkiae 11 점이 aflatoxin과 ochratoxin A을 각각 생성하였고 일부 균주는 높은 독소생성능을 보였다. 이 결과는 새싹삼 생산에 있어 곰팡이 발생에 대한 지속적인 모니터링 및 관리방안이 필요함을 시사하였다.

Resistance to extended-spectrum cephalosporin in Enterobacteriaceae is increasingly prevalent in South Korea. This study aims to explore the distribution of AmpC genes in Proteus mirabilis isolated from stray and hospital-admitted companion animals in South Korea. AmpC β-lactamases hold clinical significance due to its potential to facilitate antimicrobial resistance to cefoxitin, cefazolin, and most penicillins. A total of 163 bacterial isolates belonging to the Enterobacteriaceae family were collected from dogs (n = 158) and cats (n=5). Of them, 134 isolates were from hospital-admitted animals, while 29 isolates from stray animals. Boronic acid tests and antimicrobial susceptibility tests were conducted for an initial screening to detect AmpC β-lactamase resistant P. mirabilis. Gene-specific PCRs were conducted to identify the type of AmpC genes, which include six groups (MOXM, CITM, DHAM, ACCM, EBCM, and FOXM), in the resistant isolates. The boronic acid disk tests revealed 45 (27.6%) positive isolates out of 163 isolates tested. Of these 45 isolates, six were determined to harbor the EBCM gene, 13 for CITM, one for FOXM, and one for DHAM by single detection PCR. No isolates carried for ACCM or MOXM. Thus, a total of 21 out of 163 isolates (12.9%) were demonstrated to possess AmpC genes. No isolates contain more than one group of AmpC gene family. A significantly higher percentage of P. mirabilis was found to possess AmpC genes compared to past studies. Therefore, the increasing trend in antimicrobial resistance in P. mirabilis indicates a dire need to monitor antimicrobial prescription in the veterinary field.