Nuclear power plants use ion exchange resins to purify liquid radioactive waste generated while operating nuclear power plants. In the case of PHWR, ion exchange resins are used in heavy water and dehydration systems, liquid waste treatment systems, and heavy water washing systems, and the used ion exchange resins are stored in waste resin storage tanks. The C-14 radioactivity concentration in the waste resin currently stored at the Wolseong Nuclear Power Plant is 4.6×106 Bq/g, exceeding the low-level limit, and if all is disposed of, it is 1.48×1015 Bq, exceeding the total limit of 3.04×1014 Bq of C-14 in the first stage disposal facility. Therefore, disposal is not possible at domestic low/medium-level disposal facilities. In addition, since the heavy water reactor waste resin mixture is stored at a ratio of about 20% activated carbon and zeolite mixture and about 80% waste resin, mixture extraction and separation technology and C-14 desorption and adsorption technology are required. Accordingly, research and development has been conducted domestically on methods to treat heavy water waste resin, but the waste resin mixture separation method is complex and inefficient, and there are limitations in applying it to the field due to the scale of the equipment being large compared to the field work space. Therefore, we would like to introduce a resin treatment technology that complements the problems of previous research. Previously, the waste resin mixture was extracted from the upper manhole and inspection hole of the storage tank, but in order to improve limitations such as worker safety, cost, and increased work time, the SRHS, which was planned at the time of nuclear power plant design, is utilized. In addition, by capturing high-purity 14CO2 in a liquid state in a high-pressure container, it ensures safety for long-term storage and is easy to handle when necessary, maximizing management efficiency. In addition, the modularization of the waste resin separation and withdrawal process from the storage tank, C-14 desorption and monitoring process, high-concentration 14CO2 capture and storage process, and 14CO2 adsorption process enables separation of each process, making it applicable to narrow work spaces. When this technology is used to treat waste resin mixtures in PHWR, it is expected to demonstrate its value as customized, high-efficiency equipment that can secure field applicability and safety and reflect the diverse needs of consumers according to changes in the working environment.

국내 수출입 물동량의 증가와 해운산업의 발달에 따라 항만시설물의 사용빈도 또한 증가 추세에 있으나, SOC의 해운항만 부문의 투입 정부예산은 감축되어 왔다. 증가하는 사용빈도에 반하여 줄고 있는 예산으로 인해 항만시설물의 체계적이고 효율적인 유지관리 및 운영이 필요하다. 효율적인 유지관리 시스템 구축을 위해서 항만시설물이 위치한 지역, 구조물의 형태 및 취급화종, 시공 및 유지관리 수준과 같은 특성을 고려한 열화모델 개발이 필요하다. 항만시설물의 열화모델 개발은 시설물의 열화요인 분석과 열화데이터 수집 및 열화 모델 개발의 과정으로 수행하였다. 열화 모델 개발기법은 변수 특성에 따른 시간 의존적 상태변화를 반영할 수 있는 결정론적 방법인 다중 회귀분석과 변동성이 큰 자료들의 상태이력을 반영할 수 있는 확률론적 방법인 마코브 체인 이론을 이용하였다. 각 방법을 통해 잔교식 구조물과 블록식 구조물의 Project level의 상태 열화모델을 제시하였다.

Mealworms, Tenebrio molitor (L.) is used as an important animal feed additive for growth promotion and health management, but potentially exposes to fungal infection. In this work, virulence of two species of entomopathogenic fungi against the insect, and the relationship between abiotic features and virulence were investigated. Secondly our consideration was also given to the effect of chemical fungicides on conidial germination for risk control. Between Beauveria bassiana (Bb) and Metarhizium roberstii (Mr) (previously M. anisopliae), Bb isolates had much higher virulence (~100% mortality in 3~4 days after the treatment), rather than Mr isolates in laboratory assays. Next, fungus-treated mealworms were kept at wheat bran at 20, 25, 30 and 35℃ with 3, 6, 9 times of water spray to the feeds for set-up of different humidity conditions. Inoculation of fungi to mealworms was conducted by fungal spray and feeding methods, which resulted in higher virulence in feeding method. In the feeding method, all temperature treatments except 35℃ showed high virulence against mealworms, but any significant relationship between virulence and humidity was not observed. In the chemical fungicide screening, fluazinam (CAS No. 79622-59-6) and mancozeb (8018-01-7) significantly inhibited the germination of Bb and Mr conidia. This work suggest that contamination of wheat bran with fungal pathogens, particularly B. bassiana may induce mycosis of mealworms, but introduction of effective fungicides possibly reduce fungal infection.

Hereditary dentin defects consists of dentin dysplasia(DD) and denti nogenesis imperfecta(Dr) ‘ The Dl associated with osteogenesis imperfecta has been classified as DI type 1. whereas isolated inherited defects have been categori zed as DI types II and III , However‘ whether DI type III should be considered a distinct phenotype 01' a variation of DI type 1I is debatable , Recent genetic findings have focused attention on the role of the dentin sialo phosphoprotein(DSPP) gene in the etiology of inherited defects of tooth dentin, We have identified novel mlltation( c,727G - > A, p,D243N) at the 243th codon of exon 4 of the DSPP gene in a Korean patient with DI type III The radiographic and histologic features of the patient revealed the classic phenotype of shell teeth These findings sllggest that DI type II and III are not separate diseases bllt rather the phenotypic variation 01' a s ingle disease

본 연구는 의료용 선형가속기 시설을 차폐하는 콘크리트에 대한 중성자 방사화 연구로써, 일반 콘크리트 와 저 방사화 콘크리트를 비교 분석하였다. 실험 방법은 MCNPX (Ver. 2.5.0)와 FISPACT-2010를 사용하여 모의실험을 진행하여, 광자선과 중성자선에 대한 차폐능을 산정하고 중성자 방사화 평가를 진행하였다. 그 결과 차폐능은 일반 콘크리트에서 20~50 ㎝ 효율적이였으며, 방사화 평가의 경우 저 방사화 콘크리트에서 방사능이 낮게 계산되었으나, 모두 자체처분허용 농도를 초과하지 않는 수준으로 산정되었다. 이를 종합적 으로 분석한 결과 일반 콘크리트를 사용하는 것이 효율적인 것으로 판단된다.

본 연구는 원자력 발전소가 인접해 있는 부산지역 경찰관들의 원자력 사고와 방사능 테러에 대한 인식 과 대처 방안을 파악함으로써, 이와 관련한 정책 수립에 기초자료를 제공하고자 하였다. 부산광역시에 근 무하는 221명의 경찰을 대상으로 하였으며, 조직구성, 지휘능력, 여론운영 등으로 설문을 구성하여 재난대 응 인식도를 분석하였다. 그 결과, 성별 및 지휘계급에 따른 재난대응 인식은 큰 차이가 없는 것으로 나타 났다(p>.05). 연령의 경우 지휘능력에 대한 통계적 차이(p<.05)를 보였으며, 근무경력에 따른 지휘능력도 통 계적 차이(p<.05)를 나타냈다. 상관관계 분석 결과, 조직구성에 대한 인식과 지휘능력, 여론운영 3가지 항목 이 +0.5 이상으로 정적상관을 나타냈다(p<.01). 전반적으로 경찰 체제에 대해 긍정적인 것으로 나타났으며, 방사선사고 및 테러 발생 시 경찰의 업무를 잘 인식하고 있었다.

Perilla frutescens (L.) is an edible plant, not only used as s food ingredient, but also in skin cream, soaps, and medicinal preparetions. ‘Atom-Ros’, a perilla (Perilla frutescenc (L.) Britt. cv. Chookyoupjaso was developed in 1995 by 200 Gy gamma irradiation-mutagenesis. This new cultivar has high rosmarinic acid content more than two fold compare with ‘Chookyoupjaso’ control. The observed phenotypical difference was changed leaf color of the ‘Atom-Ketone’ from violet to green. The yield potential of this cultivar (123.5 kg/10a) was 2.14 fold higher than that of ‘Chookyoupjaso’ (57.65 kg/10a). The methanol extracts of ‘Atom-Ros’ were tested for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophase cells. Atom-Ros showed significant inhibition of NO production. This rosmarinic acid extracted from ‘Atom-Ros’ has a good potential to be developed as an antioxidant agent.

Perilla frutescens (L.) is an annual herbaceous and ornamental plant in the Lamiaceae family. Perilla frutescens (L.) Britt.cv.Chookyoupjaso were irradiated using a 200 Gy gamma ray in 1995. By HPLC analysis, this new cultivar significantly induced isoegomaketone content compared with ‘Chookyoupjaso’ control. The phenotypical difference was the changed leaf color of the ‘Atom-Ketone’ from violet to green. The yield potential of this cultivar (106 kg/10a) was 1.83 folds higher than that of ‘Chookyoupjaso’ (57.65 kg/10a). The methanol extracts of ‘Atom-Ketone’ inhibit nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. This extract was further partitioned using ethyl acetate (EtOAc), butanol (BuOH), and water. The EtOAc fraction (EF-Atom-Ketone) was evaluated for antiinflammatory activities. These results indicated that the EF-Atom-Ketone reduced NO production by inhibiting inducible nitric oxide synthase (iNOS) expression. The EF-Atom-Ketone treatment also significantly diminished expression of MCP-1 and IL-6. Therefore, ‘Atom-Ketone’ reveals the potential therapeutic use of bioactive

최근 순환기계 질환이 증가함에 따라 경피적 관상동맥 중재술이 관상동맥 치료에 주된 치료 방법으로 사용되고 있다. 이러한 이유로 방사선을 이용하는 중재술이 증가함에 따라 작업종사자의 방사선피폭을 증가되고 그에 따른 잠재적방사선 장해를 증가 시킬 수가 있을 것이다. 이에 본 연구에서는 방사선 차폐체인 납 커튼 유·무에 따른 슬 하부 주변선량을 측정하였다. 실험방법은 자동노출조건 60kV, 200mA, 10mS 을 이용하여 본원에 주로 사용되는 관상동맥 촬영방법을 이용하여 슬 하부 주변부 선량을 측정하였다. 결과로는 right coronary artery 검사 시, LAO 30°을 경우 납 커튼사용 유·무에 따른 방서선 방어효과는 98.4%, Cranial30° 98.3% 효과를 가진다. left circumflex coronary artery 검사 시, Caudal 30°을 경우 납 커튼 사용 유·무에 따른 방사선 방어효과는 90.2%, Caudal 30° LAO 30° 88.7%의 효과를 가진다.left anterior descending artery 검사 시, Cranial30°을 경우 납 커튼 사용 유·무에 따른 방사선 방어효과는 98.3%, Cranial30°RAO 30° 80.3%, Cranial 30°LAO 30° 98%의 효과를 가진다. OS(Spider view) 검사 시, Caudal 40° LAO 40°을 경우 납커튼 사용 유·무에 따른 방사선 방어효과는 71.2%의 효과를 가지는 것으로 나타났다. 이러한 이유로 방사선 작업종사자의 경우 방사선 차폐 보조기구를 사용을 불편하더라도 가능한 방사선 차폐도구를 이용함으로써 자신의 방사선 피폭관리에 관심을 기울려야 할 것이다.

VitE (tocotrienols and tocopherols) are micronutrients with antioxidant properties synthesized by photosynthetic bacteria and plants that play important roles in animal and human nutrition. A new mutant line, T1001-1, was isolated from in vitro mutagenized population by ionizing radiation and shown to have increased VitE contents. The total VitE content was 26% increased in the T1001-1 mutant seeds compare with cv. Dongan (wild-type). In addition, we showed that the mutant confers retarded seedling growth during the early seedling growth stage in rice. To study the molecular mechanism of VitE biosynthesis, we used the rice microarray to identify genes that are upor down-regulated in T1001-1 mutant. In addition, we identified differentially regulated pathway using MapMan analysis, which provides deep insight into changes in transcript and metabolites. Our results enhanced the transcription of genes involved in starch and lipid metabolism in T1001-1 mutant. To identify the molecular mechanisms of the events involving transcription factors in tocopherol accumulation, we compared the expression patterns of transcription factors. The AP2-EREBP, WRKY, C2H2 transcription factor were up-regulated, whereas the MYB family was down-regulated in T1001-1 mutant. Our results demonstrate change of important transcript in high level of VitE accumulating rice mutant.

Ionizing radiation is known to cause chromosomal alterations such as inversions and deletions and affects gene expression within the plant genome. To monitor the genome-wide transcriptome changes by ionizing radiation, we used rice Affimetrix GeneChip microarray to identify genes that are up- or down regulated by gamma-ray (200 Gy, 60Co source), cosmic-ray and ion beam (40 Gy, 220 MeV carbon ion). The overall expression patterns between gamma-ray and ion beam were similar but cosmic-ray was regulated differently. Combined results from all 3 radiations identified 27 up-regulated genes and 188 down regulated genes. These results mean the induction of similar mechanism changes in treatments of gamma ray and ion beam. However the different expression in treatment of cosmic-ray might be due to the other environmental conditions. Among the commonly up- or down- regulated genes, we chose highly up- or down- regulated several genes and confirmed its regulation in response to ionizing radiation exposure by RT-PCR analysis. Moreover, we showed that specific co-expression networks of candidate radio marker genes by ARACNE algorithm. Our results present profiles of gene expression related to different ionizing radiation and marker gene to predict sensitivity to ionizing radiation, such as GS (glutelin subunit) and FBX322.

Cryopreservation has been known as an efficient method for long-term preservation of clonally propagated plants, and several cryopreservation methods have been developed. Among them, a droplet-vitrification method for potato using axillary shoot tips in vitro has been established previously. In this study, we have optimized the procedure in which explants were submitted to a step-wise pre-culture in liquid sucrose-enriched medium (0.3 and 0.7 M for 7 and 17 h, respectively). The pre-cultured explants were dehydrated with PVS3 (w/v, 50% glycerol + 50% sucrose) for 90 min or modified PVS2 vitrification solution (w/v, 37.5% glycerol + 15% DMSO + 15.0% ethylene glycol + 22.5% sucrose) for 30 min. This two dehydration solutions produced post-cryopreservation regeneration percentages of 57.2% and 80.9%, respectively. We also compared a new post-culture medium (0.1 mg L ･ -1 GA3, 0.1 mg L ･ -1 kinetin) with the conventional one (0.15 mg L ･ -1 IAA, 0.2 mg L ･ -1 zeatin, 0.05 mg L ･ -1 GA3); the shooting initiation rates were 80.9% and 43.5%, respectively. The results suggest that the modified droplet-vitrification protocol described in this study is more effective, easier to implement, and more economical than the droplet-vitrification protocols currently used for potato.