Italian ryegrass (Lolium multiflorum Lam., IRG) is a widely cultivated winter forage crop known for its high yield and nutritional value. This study evaluated the processing characteristics and feeding performance of IRG-based pellets in Hanwoo cattle (Bos taurus coreanae) and Korean native black goats (Capra hircus). IRG was harvested at the optimal growth stage and processed into two pellet formulations: IRG ≥80% (with up to 20% soybean meal) and 100% IRG. Feeding trials were conducted under ad libitum feeding conditions. Hanwoo cattle showed higher intake of 100% IRG pellets (7.9 kg/day/head) than IRG ≥80% pellets (7.5 kg/day/head, p<0.05), with similar average daily gain (0.9 ± 0.4 kg/day/head). Conversely, black goats exhibited significantly lower intake of IRG ≥80% pellets (54.6 g/day/head) compared to 100% IRG pellets (266 g/day/head), likely due to reduced palatability associated with soybean meal inclusion. These findings suggest that IRG pellets are suitable for Hanwoo cattle, while further optimization of pellet size and formulation is required to improve acceptance in goats. Future studies should assess long-term impacts on digestion, rumen fermentation, and metabolic responses.

This study investigated the quality change of strip loin from hanwoo with quality grade 2 produced by dry aging, and compared the quality of dry-aged strip loin with that of strip loin from hanwoo with quality grade 1+. Hanwoo strip loins with quality grade 1+ (SL1) and 2 (SL2) after a day of slaughter and dry-aged strip loin (DSL2) aged for 21 days at 2℃ with 85% humidity were obtained from local markets. The proximate composition, pH, cooking loss, lipid oxidation, instrumental color (CIE L*, a* and b* value) and sensory properties of the strip loins were measured. DSL2 contained low moisture and high protein contents compared with SL2, and low fat, high protein, and high ash contents compared with SL1 (p<0.05). The pH and cooking loss were higher and lower, respectively, in DSL2 compared to SL2 (p<0.05). SL1 had a higher pH than DSL2 (p<0.05). However, the cooking loss was not different between SL1 and DSL2. The TBARS value was the highest in DSL2 and the lowest in SL2 (p<0.05). DSL2 had higher L* and b* value compared with SL2 (p<0.05). There were no significant differences of L*, a* and b* values between DSL2 and SL1. Sensory properties such as the color, flavor, taste, texture, and acceptability of DSL2 were higher than those of SL2 (p<0.05). There was no significant different in all sensory properties between DSL2 and SL1. According to the results, dry aging can improve the nutritional and sensorial quality of strip loin with quality grade 2 to quality comparable to quality grade 1+.

Colorectal cancer is the third most commonly diagnosed cancer in the world, nearly all patients diagnosed with this cancer die from it. Antibodies are glycoprotein molecules, which can efficiently recognize and eliminate specific pathogenic and disease antigens. Antibody researches for the last several decades have demonstrated the potential of therapeutic antibodies to fight cancer. Monoclonal antibody (mAb) CO17-1A recognizes the tumor-associated antigen GA733-2, a cell surface glycoprotein highly expressed in colorectal carcinoma cell, which is applicable for preventing and curing colorectal cancer. We have currently established baculovirus insect cell expression system to produce anti-colorectal cancer mAb CO17-1A. In this study, mAb CO17-1A was expressed in the transgenic insect cell line SWT4, which has humanized glycosylation processing pathway. Immunoblot confirmed that mAb CO17-1A properly expressed in SWT4. mAb CO17-1A was purified using protein G affinity column. In addition, Maldi-TOF verified that the mAb fused to KDEL, ER retention signal had high mannose type of glycan structure whereas the mAb without KDEL had partially humanized glycan structure. These results suggest that the insect cell expression system with the SWT4 possibly can be used as a useful alternative way to produce full-size mAb with humanized glycan structures for cancer immunotherapy.

Food web of main arthorpods in 3 differently managed rice fields (low input, duck, apple snail farming) was investigated in Hongseong area, Chungcheongnam-do, Korea. Main arthropods were selected by their abundance during the rice growing season in 2010. Food web was analyzed with stable isotope, δ15N and δ13C according to rice growth satges (seedling, tillering, booting, blooming/heading, and ripening stage). Rates of δ15N and δ13C in soil (F=5.72; df=2, 6; P=0.0407, F=40.86; df=2, 6; P=0.0003, respectively) and δ13C in rice (F=6.46; df=2, 6; P=0.0319) were statistically different. Rate of δ13C in Lissorhoptrus oryzophilus (F=9.50; df=2, 6; P=0.0138), Pirata subpiraticus (F=11.00; df=2, 6; P=0.0098), and Pachygnatha clercki (F=6.33; df=2, 6; P=0.0333) were statistically different among farming methods. Stable isotope structures in food web were slightly different among 3 farming methods seasonally. Rates of δ15N and δ13C of rice pests such as Nephotettix cincticeps, Nilaparvata lugens, Laodelphax striatellus, and Sogatella furcifera were related to rice plant. Those of Tomoceridae sp. and Chironomidae sp., however, were not related to rice plant. Spiders as main natural enemy group were ranked on the top of food web and were thought to have life cycle based on the rice field from stable isotope structure.

The faulty regulation of imprinting gene lead to the abnormal development of reconstructed embryo after nuclear transfer. However, the correlation between the imprinting status of donor cell and preimplantation stage of embryo development is not yet clear. In this study, to determine this correlation, we used the porcine spermatogonial stem cell (pSSC) and fetal fibroblast (pFF) as donor cells. As the results, the isolated cells with laminin matrix selection strongly expressed the GFRα-1 and PLZF genes of SSCs specific markers. The pSSCs were maintained to 12 passages and positive for the pluripotent marker including OCT4, SSEA1 and NANOG. The methylation analysis of H19 DMR of pSSCs revealed that the zinc finger protein binding sites CTCF3 of H19 DMRs displayed an androgenic imprinting pattern (92.7%). Also, to investigate the reprogramming potential of pSSCs as donor cell, we compared the development rate and methylation status of H19 gene between the reconstructed embryos from pFF and pSSC. This result showed no significant differences of the development rate between the pFFs (11.2±0.8%) and SSCs (13.3±1.1%). However, interestingly, while the CTCF3 methylation status of pFF-NT blastocyst was decreased (36.3%), and the CTCF3 methylation status of pSSC-NT blastocyst was maintained. Therefore, this result suggested that the genomic imprinting status of pSSCs is more effective than that of normal somatic cells for the normal development because the maintenance of imprinting pattern is very important in early embryo stage.

Infrared emissions from spherical dust, clouds are calculated using quasi-diffusion method. We have employed graphite-silicate mixture with power-law size distribution for the dust model. The grains are assumed to be heated and cooled by radiative processes only. The primary heating source is diffuse interstellar radiation field. hut the cases with an embedded source are also considered. Since graphite grains have higher temperature than silicate grains, the observed IR emission is mainly due to graphite grains, unless the fraction of graphite grains is negligibly small. The color temperature of Bok globules obtained from IRAS 60 and 100 μ m data are found to be consistent with the dust cloud with graphite-silicate mixture exposed to average interstellar radiation field. The color temperature is sensitive to the external radiation field, but rather insensitive to the size distribution of the grains. We found that the density distribution can be recovered outside the beam size using the inversion technique that assumes negligible optical depth. However, the information within the beam size is lost for if beam convolved intensity distributions are used in deriving density profile.