우리는 길앞잡이(Cicindela chinensis)의 장에서 다양한 공생 미생물들을 분리하였다. 그중 다양한 곰팡이 성장 을 억제하는 세균을 동정하였고 “Ch-1”이라 명명하였다. 우리는 Ch-1 균주를 사용하여 10종의 식물 병원성 곰팡 이와 2종 곤충 병원성 곰팡이의 생장 억제를 확인하였다. 또한 8종의 항생제에 대한 저항성을 확인하였다. 동시 에, 본 균주의 genomic sequence를 수행하였고 유전적, 생화학적, 생리적 특성을 조사하였다. Ch-1균주는 특허등 록과 친환경 미생물제제로 등록하였고 향후 생물학적 방제제로써 활용될 수 있을 것으로 판단한다.

본 연구는 게임 관련 전문가 23명에게 델파이 기법을 사용해 기존의 게임 가치평가에 추가적 요소들을 추출해 보고자 한다. 기존에 제시된 게임 컨텐츠의 평가 모델을 이용하여 개발 모델의 특성을 전반적으로 검토 한 결과, 컨텐츠를 선정 할 때 가치 평가 요소가 선정되었음을 알 수 있었으며 델파이 분석은 2차에 걸쳐서 이루어졌으며, 1차는 전문가 FGI, 2차는 델파이 설문으로 진행되었다. 결과, 기술 이전 및 보급, 상용화 촉진을 위한 세부 내용 등 관련 항목등에서 보다 세밀한 요소들이 제시되었다. 포커스 그룹 인터뷰를 통해 추가 요인을 추출하고 게임 업계의 컨텐츠 성공에 대한 시장 분석을 통해 시장 규모, 성장 잠재력, 규제 및 시장 매력과 위험을 예측 했으며 이를 통해 관련 업계는 성공 목표에 대한 시장 분석을 통해 시장 규모, 성장 잠재력, 규제, 시장 호소력 및 위험성을 이해할 수 있을 것으로 기대한다. 이 연구는 제안 된 평가 요소가 게임 및 문화 콘텐츠 산업 전 체에 적용될 수 있음을 시사하며 또한 콘텐츠의 시장 가치 평가, 목적 및 목표 설정, 진입 방법 선택, 전략 요소 등 시장 요인을 구성하는 것에 도움이 될 것이다.

Domestic continuously reinforced concrete pavement has been built on a large scale on the Jungbu Expressway, which opened in 1987. Since then, there have been few application due to cost and construction difficulty. Currently, most of the expressway concrete pavements have been applied with jointed plain concrete pavement, but the interest in continuously reinforced concrete pavement has been increasing recently due to frequent premature failure in joints of JPCP. Accordingly, Korea Expressway Corporation has established design guidelines and details for continuously reinforced concrete pavements for expressway in 2014, based on the design method applied to the Jungbu Expressway, but incorporating foreign useful design methods. In terms of construction, pilot constructions were performed on Pyeongtaek~Jecheon Expressway Construction. The new design guidelines were applied to some sections of the Sangju~Yeongdeok Expressway Construction Project in 2016. This study conducted a survey and analysis of transverse cracks for one year after continuously reinforced concrete pavement construction in Sangju~Yeongdeok Expressway. This route was constructed with pavement thickness, base layer and longitudinal reinforcement according to domestic design guidelines. However, the transverse reinforcement was arranged differently according to the section. After construction, the transverse crack spacing was surveyed and the crack gauges were installed at the crack position to measure the movement. This compares the crack spacing according to the characteristics of longitudinal reinforcement and observes continuous movement of the crack width. The results showed that the crack spacing was more affected by the climate at the time of construction than the arrangement method of the transverse reinforcement. The crack width showed periodic changes according to the temperature change over 24 hours. However, due to seasonal changes, it showed a tendency of gradual increasing or decreasing in crack widths, and it was predicted that different measurement results will be shown depending on the time of crack width measurement. Generally, it is predicted that the crack spacing will be narrowed and the crack movement will tend to converge with the period after construction. This initial crack movement data is expected to be useful for comparing whether these predictions are valid.

This study was conducted to investigate the effects of alpha-lipoic acid (aLA) as an antioxidant that decrease the reactive oxygen species (ROS) in bovine embryonic development. Slaughterhouse derived bovine immature oocytes were collected and 4 different concentrations (0, 5, 10 and 20 mM) of aLA was supplemented in bovine in Vitro maturation (IVM) medium. After 20 hrs of IVM, maturation rates, levels of ROS and glutathione (GSH), and further embryonic development after parthenogenetic activation (PA) and in Vitro fertilization (IVF) was investigated according to aLA concentrations. Maturation rate was significantly higher in 10 mM group than other groups (80.5% vs. 62.9, 73.9, 64.2%; P<0.05). In the levels of ROS and GSH in matured oocytes as an indicator of oocyte quality, significantly better results were shown in 5 and 10 mM groups compared with other 2 groups. After IVM, significantly higher rates of blastocyst formation were shown in 10 mM groups in both of PA (27.9% vs. 18.8, 22.3, 14.2%; P<0.05) and IVF (32.6% vs. 23.9, 27.3, 16.2%; P<0.05) embryos. In addition, significantly more cell total cell number and higher inner cell mass ratio in 10 mM PA and IVP blastocysts showed developmental competence in 10 uM groups. Therefore, based on the entire data from this study, using 10 μM of aLA confirmed to be the optimal concentration for bovine oocyte maturation and embryonic development.

The objective of this experiment was to explore the effects of Roscovitine (Rosco) prior to in vitro maturation (IVM) of immature pig oocyte. Brilliant cresyl blue test has been used to select the good quality of oocyte. Specifically, the effects of Rosco exposure on nuclear and cytoplasmic maturation, diameter, intracellular glutathione (GSH) and reactive oxygen species (ROS), and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT), and gene expression levels in SCNT embryos have been measured. Cumulus oocyte complexes (COCs) have been exposed in 75 μM of Rosco for 22 and 44 h. The COCs that were matured in the IVM for 44 h without Rosco used as control group. Diameter of matured porcine oocytes 44 h culture with Rosco was significantly lower than 22 h culture with Rosco and control groups. GSH was higher in control group than 22 h and 44 h with Rosco but reduction of ROS in 22 h than 44 h with Rosco. In PA, exposure with Rosco 44 h oocytes group has been significantly lower than 22 h and control group in rates of maturation, cleavage and blastocyst formation. Similarly, in SCNT embryos rates of maturation, cleavage and formation of blastocyst have been also significantly lower in 44 h Rosco treated group than other two groups. SCNT embryos treated with Rosco 22 h showed greater expression levels of POU5F1, DPPA2 and NDP52Il mRNA compared with other two groups. Our results demonstrate that Rosco treatment with 22 h prior to IVM improves the development competence of porcine oocyte.

This study was conducted to establish the optimal chemical post-activation conditions in porcine embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) using 4 different chemical compositions (cytochalasin B (CB), cyclohexamide (CHX), demecolcine (DC), 6-dimethylaminopurine (DMAP). Porcine embryos were produced by PA and SCNT and then, cultured for post-activation with CB (7.5 μg/mL), CB (7.5 μg/mL) + CHX (10 μg/mL), CB (7.5 μg/mL) +DC (0.4 μg/mL), and CB (7.5 μg/mL) + DMAP (2 mM). In PA embryonic development, cleavage rates have been significantly higher in CB group (94.7%) and CB+DMAP group (94.1%) than that of CB+CHX and CB+DC group (88.1 and 84.3%, respectively). There have been no significant differences in blastocyst formation rates among the four groups. In cell number of blastocyst was shown in CB group (42.3%) significantly higher than CB+CHX and CB+DC group (40.6 and 40.6%, respectively). In SCNT embryonic development, CB+DMAP group (89.7%) significant differences were found on embryo cleavage rates when compared with other three groups. Blastocyst formation rates in CB+DMAP group (26.9%) were significantly higher when compared with CB, CB+CHX, and CB+DC groups (25.5, 20.2, and 22.1%, respectively). In blastocyst cell number, CB+DMAP group (41.4%) was found higher significant difference compared with other three groups. Additionally, we have investigated survivin expression in early development stages of porcine SCNT embryos for more confirmation. Our results establish that CB group and CB+DMAP group for 4 h during post-activation improves pre-implantation improvement of PA and SCNT embryos.

The purpose of this study is to observe the effect of elasticity of taping on ankle muscles’activity and endurance after plyometric training that easily causes ankle injury, and provide baseline data for physical therapy intervention methods. The study subjects are 24 male students in their 20s who attend N University in Choongnam. They were divided into three groups; 8 subjects in the elastic taping group, 8 in the non-elastic taping group, and 8 in the non-taping group(control group). They had plyometric training for 6 weeks. After the training, this study measured their maximum voluntary isometric contraction(MVIC) and muscle endurance of the muscles around ankle joint. The experiment result is as follows. After the training, all three groups showed improvement in muscle strength and endurance. The elastic taping group showed insignificant improvement in muscle strength but significant increase in muscle endurance in plantarflexion. In dorsiflexion, both muscle strength and endurance increased significantly. The non-elastic taping group showed insignificant improvement in muscle strength but significant increase in muscle endurance in plantarflexion. Taping during plyometric training had a little or insignificant effect on muscle endurance and strength compared to the non-taping group.

사용후 핵연료내 우라늄 및 초우란원소를 회수하는 파이로프로세싱 공정에서 배출되는 금속염화물계 방사성 폐기물은 높은 휘발특성과 붕규산계 유리와의 낮은 상용성으로 인해 고화처리가 쉽지 않은 폐기 물이다. 이를 위해, 본 연구에서는 고화처리의 한 방법으로 탈염화 반응을 통한 고화체제조 개념을 채택 하였다. 솔젤법을 이용하여 탈염화물질, SiO2-Al2O3-P2O5 (SAP)을 합성하였으며 이를 이용하여 탈염화 반 응거동 반응생성물의 고형화 특성을 조사하였다. LiCl계 폐기물과 달리, LiCl-KCl폐기물의 반응은 두 개 의 온도범위에서 반응이 진행되며, 400℃의 경우에는 LiCl이, 약 700℃에서는 KCl이 주로 반응하는 것으 로 확인되었다. 여러 가지 반응실험을 통하여 LiCl-KCl의 탈염화 반응에 가장 적합한 물질은 SAP 1071 (Si/Al/P=1/0.75/1 in molar)인 것으로 확인되었다. 4가지 종류의 고형화 실험을 통하여 고화체의 bulk shape과 densification은 SAP/Salt의 비에 영향 받는 것을 확인하였다. 제조된 고형화 시료는 Product Consistency Test-A법을 이용하여 기본적인 내구성을 평가하였다. 본 연구는 SiO2, Al2O3, P2O5 로 이루 어진 탈염화 물질을 이용하여 반응특성과 고형화 특성에 대한 기본적인 정보를 제공하였으며, 이와 같은 실험을 통하여, 본 연구에서 제안된 탈염화 고화처리방법이 휘발특성이 높고 기존 유리매질과 상용성이 낮은 금속염화물계 폐기물에 적용이 가능함을 확인하였다.

금속염화물계 방사성 폐기물은 전해공정으로 이루어진 파이로프로세싱공정의 주요한 방사성 폐기물이 다. 이와 같은 폐기물은 탄산염이나 질산염과 달리 고온에서 분해되지 않고 바로 휘발되며, 기존의 규산 계 유리와 상용성이 낮아 처리가 쉽지 않다. 본 연구팀은 금속염화물계 폐기물을 고화처리하는 방법으로 탈염화처리법을 채택하였다. 본 연구에서는 그 후속적인 연구로서, 탈염화물질로 제안된 SAP (SiO2- Al2O3-P2O5)의 조성을 변화시켜 LiCl-KCl과의 반응성을 향상시키고 고화공정을 단순화시키고자 하였다. 기본물질계에 Fe2O3를 첨가할 경우 무게반응비 SAP/Salt를 3에서 2.25로 낮출수 있으며, Fe가 Al을 치환 하는 몰분율이 0.1이상이 될 경우에는 오히려 반응성이 점진적으로 감소하는 것으로 확인되었다. 또한 M-SAP에 B2O3를 첨가할 경우에는 유리매질을 사용하지 않고 monolithic form을 제조할 수 있었다. 침출 시험결과 U-SAP 1071이 가장 높은 내구성을 보여주었으며, 1 g의 금속폐기물을 처리시 약 3∼4 g의 고 화체가 발생되며, 이는 기존의 고화처리법보다 약 ⅓∼¼배정도 최종처분부피가 감소되는 효과를 얻을 수 있다. 이상의 실험결과로부터, 기존의 유리고화공정으로 처리가 어려운 휘발성 금속염화물계 폐기물 을 단 하나의 물질을 이용하여 처리할 수 있음을 확인하였으며, 이러한 처리방법은 고화처리시 발생되는 부피를 최소화활 수 있는 대안적인 고화처리방법이 될 것으로 판단된다.

This study is on a seismic analysis of ab50rber rod in KMRR Reactivity Control Mechanism. The model being studied is two coaxial tubes(control absorber rod and flow tube) immersed in the water and partially coupled(overlap) by water gap. The hydrodynamic mass effects by the water in 않ch surrounding conditions are considered in the model. The natural frequencies, stresses and displacements of the system due to Safe Shutdown Earthquake are computed in the cases of in-phase m여es and out-of-phase modes of two coaxial tubes. The results show that maximum stresses are well below the allowable limit but the maximum displacements at the ends of both tu∞s are 50 much that the ab50rber rcxi contacts with the flow tube(or surrounding wall).