Despite the recent increase in the consumption level of the processed meat-byproducts, the health and safety issue has consistently been raised in the processes of production, distribution and consumption. The purpose of this study is to analyze and evaluate the microbiological hazard elements in the Korean sausage, “Sundae,” to present not only the safety standard of meat by-product vendors based on HACCP (Hazard Analysis Critical Control Point), but also the quality control criteria and sanitary arrangements of small manufacturers. For the study, the microbiological hazards in 24 raw materials, 7 manufacturing processes, 40 facilities and tools, 17 workplace environment, and 12 workers were analyzed. The analysis revealed the hazardous elements in the initial stages with 6.28 and 4.07 log CFU/g of total aerobic count and coliforms, respectively, detected from the porcine blood and 3.23 log CFU/g of coliforms from the porcine small intestines. The result also showed that the total aerobic counts and coliforms in the process of mixing and filling process exceeds the standards in the hygiene guidelines by Natick with the total aerobic counts of 5.23, 5.45 log CFU/g, and the coliforms of 3.25, and 3.31 log CFU/g, respectively. Although the detected total aerobic count and the coliforms in the filling and washing rooms exceeded the standards, it was found that the total aerobic count was significantly reduced by 98% after cleaning and disinfecting and no coliforms was detected in any process thereafter. In order to achieve high level of safety in the manufacturing processes of Sundae, the separation of washing and disinfection room from the other sections and the sanitation control of the workers must be preceded, along with strict monitoring in the storage and distribution processes. The study raises necessity for additional studies for the safety evaluation of the processed meat-byproducts and further researches on the validity of the critical limits.

본 연구에서는 항산화 및 미백 소재의 개발을 위해 다양한 추출법을 이용하여 용담화 추출물을 제조하고 항산화 효과 및 멜라닌 생합성 저해능을 평가하였다. 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거활성을 평가한 결과 용담화 추출물의 항산화 활성은 모두 농도 의존적으로 증가하였다. 또한 용담화 추출물은 tyrosinase 활성을 저해시켰으며, B16F10 세포에서 멜라닌 생성을 감소시키는 효과를 보였다. 멜라닌 생합성에 관여하는 유전자의 발현에 미치는 영향을 알아보기 위해 tyrosinase-related protein (TRP)-1 및 2 mRNA 발현을 확인한 결과 용담화 추출물에 의해 TRP-1 및 2의 발현이 감소되는 것을 확인할 수 있었다. 이러한 결과로 보아 용담화 추출물은 항산화 및 미백 기능성 화장품 소재로서의 응용이 가능할 것으로 사료된다.

It was conducted to provide a basis for the development of healing programs utilizing the existing infrastructure of the rural education farm in the process of looking for early application plan of ‘Agro-healing’. The purpose of operating the farm was in order production･ process･sales and education, and then, healing. The production･process･sales was the highest (p=0.000). All farms have had signs or bulletin board, parking. Gender-separated toilets, and basin was held 90% or more. The farm which was held cultivation facilities for disabled in wheelchair was found to be 9.6%. Regular employee was minimum 1 people including owner up to 10 people, an average of 3.7 people were working, a welfare horticultural therapist is the highest as a retain qualification. The purpose of operating program in the farm was education, it was the highest. The most utilizing resources were agricultural work for operation the programs (p=0.000). The program was developed by owner themselves in the most of farms. Regular employment was found to be 6.7%. The age of participant was in order elementary schoolchild and then toddlers. Group form of visiting was school unit or work unit mostly. Target who needed help appeared to alcohol drug addicts and then handicapped, the number of people of participating at the same time was minimum 10 to 100 people. 79% of respondents had the intend of introducing healing program and transition to healing farm. Subject who respondents wanted to access for the next healing program was elementary schoolchild. Number of participants which respondents wanted as a group was 15. Most general people wanted the emotional experience which stimulated the emotion and mood such as positive emotions, joy, and pride. If we introduce the program for healing utilizing the infrastructure of the rural education farm, it might contribute to explore new spaces for creative agriculture and the early settlement of ‘Agro-healing’.

본 연구에서는 해파리로부터 콜라겐 추출물을 제조하였고, 이에 대한 보습 효과를 측정하였다. 보습 효과 는 caspase 14 발현, filaggrin, hyaluronan synthase-3 (HAS- 3), aquaporin-3 (AQP-3) 및 desmocollin (DSC)을 측정하였다. 그 결과, caspase 14 mRNA 발현은 비교물질인 retinoic acid (RA)와 유사한 효 과를 나타냈다. 그리고 콜라겐 추출물은 20 mg/mL의 농도에서 각각 211.7%, 139.9%, 212.5% 및 116.8%의 filaggrin, HAS-3, AQP-3 및 DSC 발현 증가를 나타냈다. 따라서, 본 연구에서 해파리 콜라겐 추출물은 보습 효과를 갖는 화장품 소재로서의 개발 가능성이 클 것으로 기대할 수 있다.

본 연구는 그린인테리어용 식물을 활용한 실내 주거공간의 심리적 효과를 알아보기 위해 112명을 대상으로 설문조사하였다. 심리상태측정은 주의회복척도, 단축판 기분상태검사(POMS), 어의구별척도법(SD법)으로 조사하여 빈도분석, 일원배치분산분석, 그리고 Pearson’s 상관분석으로 비교 분석하였다. 그린인테리어에 대한 일반적 인식에 대해 조사한 결과, 그린인테리어 조성시 경관디자인과 식물의 종류를 중요하게 생각하였으며, 설치 투자비용은 200만원 미만이 80.93%, 유지관리비 10~50만원이 81% 이상이었다. 또한 3가지 심리적 지표를 분석한 결과, 주의회복척도는 전체 문항에서 ‘매우 그렇다’고 답변한 대상자의 비율이 높은 것으로 나타났다. 직업군별로 비교한 결과, 회사원보다 주부들의 주의회복력이 더 높았으며 두 직업군 간에 유의한 차이를 보였다. 기분상태검사는 긴장－불안, 우울, 분노, 피로, 그리고 혼란은 낮게, 활력은 높은 경향이었다. 또한 그린인테리어 실내 주거공간에 대한 감성을 SD법으로 알아본 결과, ‘쾌적감, 자연감, 진정감’ 모두 긍정적으로 유의하게 높은 점수 분포를 나타냈다. 3가지 감성어휘에 대한 상관관계를 분석한 결과, 그린인테리어 주거공간에 대해 모든 감정간에 유의하게 높은 상관관계를 보였다. 그린인테리어가 주는 효과에 대한 응답자의 인식을 분석한 결과, 공기정화효과, 거주자의 작업 능률 향상 효과, 심리적 안정 효과, 그리고 인체의 자연치유력을 높이는 효과에 대해 94% 이상으로 긍정적인 반응을 보였다. 그린인테리어효과의 문항간 상관분석 결과, ‘그린인테리어는 심리적 안정 효과를 높여준다’와 ‘그린인테리어는 공기정화효과를 높여준다’는 0.818로 가장 높은 정의 상관관계를 나타냈다. 본 연구결과, 그린인테리어용 식물을 활용함으로써 거주자들의 심리적 안정과 부정적 정서완화 효과가 클 것으로 기대한다.

‘Haeoreum’, a new variety of Hibiscus syriacus was selected from the progenies of artificial breeding between ‘Bulkot’, as a mother plant, and ‘Wonhwa’, as a pollenizer, at the Korea Forest Research Institute in 2006. The preliminary, advanced and regional trials for evaluation and selection of this variety were carried out from 2012 to 2013. Investigated morphological characteristics showed that ‘Haeoreum’ is a simple flower type and has bell shape in open type of corolla. The diameter of flower is about 12.8mm, and the white petals are slightly folded each other. The length of red eye zone is very long, about 26.0mm. Leaf type is in oval shape, and leaf thickness, glossy level and the shape of leaf base is medium, medium and rounded, respectively. Its blooming period each year lasted more than 80 days, and the quantity of bloom is large in normal condition. In addition, it showed faster growth performance compared to the similar varieties, about 90cm per growing season in tree height. According to all these characteristics, the new variety ‘Haeoreum’ could be useful for large container plants or street trees.

Over the last decades, increasing natural disasters and climate change are considered as the major environmental problems facing the globe. Numerous studies have been indicated it would cause huge losses on agriculture, especially in the grain productivity. Therefore, several alternatives are suggested for boosting up productivity of wheat as one of the main human food crop. One of important strategy is proper management of inflorescence development and DELLA proteins have been elucidated to play pivotal roles in growth of many plant organs. In this study, putative negative regulator of DELLA protein, GAST (Gibberellic acids stimulated transcript) have been isolated to identify their role in the developing spike of wheat. Four genes were isolated from its gene family and designated as TaGAST1, 2, 3, 4. Genomic structure was analyzed to demonstrate chromosomal localization of TaGAST genes and evolutionary relationships were also verified with GAST genes in other plant species. RT-PCR was conducted to detect transcriptional changes of TaGAST genes on external phytohormone. Each of TaGAST genes showed considerable changes in transcription level after GA, ABA, PAC treatment, respectively. Through Yeast two-hybrid assay, one protein for TaGAST1, and four proteins for TaGAST2 was isolated as putative interactive proteins in wheat spikes just before and after emergence.

Rye has important genes for biotic and abiotic stress resistance. Introduction of these genes to wheat by breeding wheat-rye translocation have been intensively used in wheat breeding program. Rye chromatin 1RS and/or 2RL show superior performance in unfavorable environments. In order to develop high yielding wheat, we applied various molecular breeding strategies. To develop EST-derived 1RS specific markers, we used comparative genomics with public sequence databases of Poaceae family. Putative rye chromatin specific sequences were used to design 1RS specific markers. To identify genes related to water deficiency, cDNA AFLP analysis was used in PEG treated seedlings of 1RS RILs. For functional analysis of identified genes and markers, we used Brachypodium distachyon, as a new model plant of temperate grasses. B. distachyon were recently applied for transformation and we constructed Agrobacterium-mediated transformation system. Integration of those strategies and conventional breeding method would enhance the usefulness of rye chromatins for wheat improvement.