인간이나 환경에 해로운 영향을 주는 지속성 유기오염물질의 독성을 평가하기 위해서 염분과 온도에 대해 내성이 강한 Acartia 종들과 Artemia을 대상으로 실험하였다. 지속성 유기오염물질인 PAHs와 TBT에 대한 요각류의 독성을 평가하기 위해 3가지의 실험을 실시하였다. 1) 광양만에서 주로 나타나는 5가지 PAHs(anthracene, benzo〔a〕pyrene, fluoranthene, phenanthrene, pyrene)에 대한 A. omo

유기주석화합물은 농업과 산업분야에서 많이 쓰이고, 이들은 수계환경으로 유입되어 물이나 저질 중의 세균들에 의해 분해되어 최종적으로는 무기주석으로 된다. Trialkyltin 화합물이 해양생물에 미치는 영향에 관해서는 많은 연구가 있지만, 해양생태계에서 기초적인 생산을 담당하는 플랑크톤류에 미치는 영향에 관해서는 잘 알려져 있지 않다. 본 연구에서는 TBT와 TPT의 분해물인 DBT, MBT, DPT, MPT를 비롯하여 trimethyltin(TMT)과

유기주석화합물은 독성이 강하고 잔존력이 감하여 수계환경으로 유입되면 해양생태계에 오랫동안 영향을 미친다 이들 화합물이 해양생불에 미치는 영향에 관해서는 어류나 패류 등의 산업적 가치가 큰 생물종을 대상으로 한 연구는 적지 않지만, 해양생태계에서 먹이사슬의 가장 하부에 위치하며 기초생산을 담당하는 플랑크톤류에 미치는 영향에 관해서는 잘 알려져 있지 않다. 따라서 본 연구에서는 TBT류의 TBTC, TBTO, TBTA, TBTB를 비롯하여 TPT류의 TPT

연안지역의 환경오염 및 양식용수의 이용과정에 파생될 수 있는 구리의 오염에 따른 넙치의 생존, 대사율, 사료효율 및 성장율에 미치는 구리의 영향을 검토하였다. 4개의 아치사구리 농도(50, 80, 180, 320 μg L-1)에서 6주동안 실험하였다. 실험기간 동안 넙치는 대조구에서는 실험종료시 까지 사망개체가 전혀 나타나지 않아 100%의 생존율을 나타내었다. 구리 노출농도 180 μg L-

3-weeks old Commelina was transferred to and grown in Hoagland solution (± 100 μM Cd2+, 100 μM Cd2+ ＋ 100μM Ca2+, 100 μM Cd2+ ＋ 200 μM EGTA) for two weeks and then a number of physiological activities

Rapidly progressive interstitial renal fibrosis has recently been reported in young women who have been on a slimming regimen including chinese herbs. Aristolochic acid, suspected as the causal factor of this renal disease, is a well known carcinogen. It has been known that Madouling (Aristolochiae fructus) contains aristolochic acid. The objective of this study was to investigate the effects of Madouling, Madouling-tang, which are the extract mixture from 10 different chinese herbs including Madouling, and aristolochic acid on reproductive and developmental toxicity. Female rats were administered orally with the extracts of Madouling, madouling-tang, and aristolochic acid from 14 days before mating to day 17 of gestation. Madouling (8mg/kg) decreased fertility in the 8mg/kg group, but Madouling-tang and aristolochic acids did not. Significant decrease of mean fetal body weights were observed in the 16mg/kg group of aristolochic acids. External, visceral and skeletal malformation of fetuses were not observed with treatment. Histopathological examination showed the discrete damage of kidney in the 8mg/kg group of Madouling and 16mg/kg groups of aristolochic acid. In whole embryo culture, Madouling and Madouling-tang caused the retardation of growth and development of embryo in the dose of 1 g/ml and 0.02 /kg, respectively while aristolochic acids showed the similar effect in the dose of 300 /kg. These results indicate that Madouling, up to 0.05mg/kg (prescription dose to human) has no adverse effects on the fertility, reproduction and development of Sprague-Dawley rats.

The morphological patterns and the cytopathogenicity time of the Vero cells induced by free Cd2+ and pigment-bound Cd2+ were observed by inverted microscope in order to investigate the difference of cadmium toxicity. The Vero cells induced by He

생태계를 평가하기 위한 평가법으로 주로 사용되는 이화학적 측정 평가법은 객관적인 측정치를 제시하는데는 용이하다. 그러나 단지 측정치의 단순한 평균이나 최고치만으로는 타 인자와의 복합 효과 또는 지속 시간에 의한 가중 효과 때문에 실상을 나타낼 수 없는 경우가 많다. 이에 따라 이화학적 측정치들을 객관적으로 종합하여 생태계의 상태를 파악할 필요성이 보다 중요하다. 즉 key factor나 trigger factor와 같은 생물체의 내성과 관련이 있는 인자

Ag와 Cu로 오염된 퇴적물이 이매패류 Macoma balthica에 미치는 만성독성영향과 주요한 중금속 흡수경로를 평가하기 위해, 실험실에서 미소생태계 실험이 수행되었다. 실험생물인 M. balthica는 4개의 농도구배를 갖는 Ag(0.01-0.87μmol g-1)와 Cu(0.75-5.55μmol g-1)로 오염된 퇴적물에서 90일 동안 배양되었다. 퇴적물 내 중금속의 지화학적 분포 특성과 생물이용도를 조절한다고 알

3주간 생장한 닭의장풀을 Hoagland용액에서 3주 동안 수경배양하면서, 100μM Cd2+과 100μM salicylic acid(SA)를 처리한 후 성장, 엽록소 함유량, 수분퍼텐셜, 엽록소 형광, 광합성능 및 기공전도도를 조사하였다. Hoagland용액에 Cd2+ 처리시 3주 동안 2.1cm생장했으나, 대조구는 7.2cm생장했다. 반면에 Cd2+ + SA 처리구는 1주 동안 0.7cm자랐으며, 2주 후에는

In order to investigate the distribution of paralytic shellfish poison, we examined the toxicity during from February to October in 2000. Of 591 shellfish samples, 17(2.88%) samples were detected. Scapgarca broughtonii was highest collected 14.29%(2/14). In the monthly detection rate of PSP, April was highest 13.3%(8/60), in the regional collecting rate, Cheon-nam coastal area was highest 3.82%(10/262), and in cases of imported area, China was 8.3%(1/12). Imported area as well as domestic area samples should be strengthen to examine enduringly.

Epoxidized soy bean oil (ESBO) is a plasticizes of PVC which is being widely used as a gaskets for the lid of glass jars including baby food. Using reverse mutation assay, chromosome aberration test and micronucleus test, ESBO were evaluated the mutagenicity. In the reverse mutation test, ESBO did not induced mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA102 with and without metabolic activation. In the chromosome aberration test using CHL cells, the results showed no increased structural and numerical aberrations in the concentration of sample producing cytotoxicity with and without metabolic activation. The in vivo induction of micronuclei was measured in polychromatic erythrocytes of bone marrow of young (3 weeks old) and adult (6 weeks old) ddY mice of both sex. At 24 hours after treatment with ESBO 20, 10, 5, 2.5 g/B.W. kg/corn oil 10 ml by oral route animals were sacrificed and bone marrow cells were prepared for smear slides. The results showed no increased micronucleated polychromatic erythrocytes regardless of sex and age. It was concluded that water soluble ESBO did not show certain genotoxicity within our studies conducted.

The toxicity and toxin composition between blue mussel, Mytilus edulis and oyster, Crassostrea gigas collected at Woepori in Koˇ je island in South Coast of Korea in 1996 and 1997 were compared. The highest toxicity score was about 10 times higher in blue mussel than oyster (blue mussel, 8,670 ㎍; oyster, 860 ㎍ in 1996, blue mussel, 5,657 ㎍; oyster, 531 ㎍/100 g in 1997). The blue mussel also retained its toxicity for slightly longer period than oyster. In the both shellfish, PSP was composed almost exclusively of C toxins (C 1 and C2, 2065%) and gonyautoxins (GTX1, 2, 3 and 4, 38-78%). In the early period of toxin accumulation, the ratio of 11(3-epimer toxins (C2, GTX4) whose amount was 2556 mole% (5th March to 12th April in 1996) and 2580 mole% (18th March to 7th April in 1997), were higher than that of llα-epimer toxins (C1, GTX2) in both shellfish. As the lapse of intoxification time, however, the ratio of 11-epimer toxins (C1, GTX2) whose amount was 41-57 mole% (27th May to 3rd June in 1996) and 25-56 mole% (29th April to 12th May in 1997), became higher than that of 11-epimer toxins. The toxin compositions in the both samples changed on a daily basis, presumably owing to metabolism of the toxins in the bivalves.