For application in nuclear decommissioning, underwater laser cutting studies were conducted on thick stainless-steel plates for various cutting directions using a 6 kW fiber laser. For cutting along the horizontal direction with horizontal laser irradiation, the maximum cutting speed was 110 mm∙min−1 for a 48 mm thick stainless-steel plate. For cutting along the vertical direction with horizontal laser irradiation, a maximum speed of 120 mm∙min−1 was obtained for the same thickness, which confirmed that the cutting performance was similar but slightly better. Moreover, when cutting with vertically downward laser irradiation, the maximum cutting speed was 120 mm∙min−1 for a plate of the same thickness. Thus, the cutting performance for vertical irradiation was nearly identical to that for horizontal irradiation. In conclusion, it was possible to cut thick stainless-steel plates regardless of the laser irradiation and cutting directions, although the assist gas rose up due to buoyancy. These observations are expected to benefit laser cutting procedures during the actual dismantling of nuclear facilities.

Pacific herring, Clupea pallasii, a keystone species with significant ecological and commercial importance, is declining globally throughout much of its range. While traditional fishing equipment methods remain limited, new sensitive and rapid detection methods should be developed to monitor fisheries resources. To monitor the presence and quantity of C. pallasii from environmental DNA (eDNA) extracted from seawater samples, a pair of primers and a TaqMan® probe specific to this fish based on mitochondrial cytochrome b (COB) sequences were designed for the real-time PCR (qPCR) assay. The combination of our molecular markers showed high specificity in the qPCR assay, which affirmed the success of presenting a positive signal only in the C. pallasii specimens. The markers also showed a high sensitivity for detecting C. pallasii genomic DNA in the range of 1 pg~100 ng rxn-1 and its DNA plasmid containing COB amplicon in the range of 1~100,000 copies rxn-1, which produced linear standard calibration curves (r2=0.99). We performed a qPCR assay for environmental water samples obtained from 29 sampling stations in the southeastern coastal regions of South Korea using molecular markers. The assay successfully detected the C. pallasii eDNA from 14 stations (48.2%), with the highest mean concentration in Jinhae Bay with a value of 76.09±18.39 pg L-1 (246.20±58.58 copies L-1). Our preliminary application of molecular monitoring of C. pallasii will provide essential information for efficient ecological control and management of this valuable fisheries resource.

Aluminum (Al) stress in acidic pH is known to decrease the growth and productivity of alfalfa. However, not much is known about how the application of silicon (Si) affects the Al stress response in alfalfa. This study was conducted to evaluate the effect of exogenous application of Si on the growth of alfalfa seedlings exposed to Al stress in pots. Alfalfa seedlings grown in pots for 2 weeks were treated either Al stress (pH 4.0, 0.2 mM Al) or Al stress + Si (1 mM) for 5 days, lengths and biomass of shoot and root, and chlorophyll and carotenoid contents in leaf tissues were analyzed respectively. Al stress treatment inhibited shoot and root growth, and decreased fresh and dry weights, and chlorophyll content in leaves, but increased carotenoid content. In contrast, when alfalfa seedlings treated with Al stress combined with Si, delayed growth caused by Al stress of shoot and root of alfalfa seedlings was restored, dry weight was increased and chlorophyll content of leaf tissue was increased, but carotenoid content was decreased. These results suggest that Si has a function of alleviating Al toxicity in alfalfa, of which it exhibits a mitigating effect by a function that overlaps with some of the intracellular functions of carotenoids.

Recently, the demand for atypical structures with functions and sculptural beauty is increasing in the construction industry. Existing mold-based structure production methods have many advantages, but building complex atypical structures represents limitations due to the cost and technical characteristics. Production methods using molding are suitable for mass production systems, but production cost, construction period, construction cost, and environmental pollution can occur in small quantity batch production. The recent trend in the construction industry calls for new construction methods of customized small quantity batch production methods that can produce various types of sophisticated structures. In addition to the economic effects of developing related technologies of 3D Concrete Printers (3DCP), it can enhance national image through the image of future technology, the international status of the construction civil engineering industry, self-reliance, and technology export. Until now, 3DCP technology has been carried out in producing and utilizing residential houses, structures, etc., on land or manufacturing on land and installing them underwater. The final purpose of this research project is to produce marine structures by directly printing various marine structures underwater with 3DCP equipment. Compared to current underwater structure construction techniques, constructing structures directly underwater using 3DCP equipment has the following advantages: 1) cost reduction effects: 2) reduction of construct time, 3) ease of manufacturing amorphous underwater structures, 4) disaster prevention effects. The core element technology of the 3DCP equipment is to extrude the transferred composite materials at a constant quantitative speed and control the printing flow of the materials smoothly while printing the output. In this study, the extruding module of the 3DCP equipment operates underwater while developing an extruding module that can control the printing flow of the material while extruding it at a constant quantitative speed and minimizing the external force that can occur during underwater printing. The research on the development of 3DCP equipment for printing concrete structures underwater and the preliminary experiment of printing concrete structures using high viscosity low-flow concrete composite materials is explained.

본 연구에서는 가리맛조개 (S. constricta)의 토사물을 현미경 검경과 차세대염기서열분석 (NGS) 기법으로 먹이원을 확인하고, 이를 통해 형태학적 및 분자학적 방법에 따른 먹이원 분석을 비교하였다. 가리맛조개 (S. constricta)의 먹이원은 분석방법에 따라 차이를 보였다. 먹이원생물은 위 내에서 분해되어 현미경 분석을 통한 생활사 확인과 정량적 분석이 가능하였으나 형태학적 및 해부학적 특성 파악이 불완전하였다. NGS 분석은 유기물 형태로 잔존하는 생물의 DNA 확인이 가능하여 현미경 검경 결과와의 상호보완적 적용 가능성을 확인하였다.

동물플랑크톤이 식물플랑크톤을 선택적으로 섭식하는 특성에 대한 이해는 수생태계 먹이사슬 내의 물질 이동에 중요하다. 하지만 해부를 통한 위내용물 추출 방법은 소형 요각류를 대상으로 적용하기에는 적절하지 않고, 유전자가 유실되거나 위내용물이 아닌 개체 외부의 유전자로 인해 오염될 가능성이 존재한다. 본 연구에서 호내 식물 플랑크톤 조성 및 기타 환경이 상이한 두 지점을 선정하여 모든 지점에서 지속적으로 출현하는 기수성 요각류인 Sinocalanus tenellus를 대상으로 위내용물의 유전자 분석을 수행하였다. 요각류 개체 외부의 DNA를 제거하는 데 2.5%로 희석한 시판용 표백제(차아염소산나트륨 5.4%) 에 2분간 처리하여 증류수로 2회 세척한 뒤 유전자를 추출하였다. 추출된 유전자는 23S rRNA을 증폭하여 서열분석을 실시하였다. Capillary sequencing 분석 결과, 원수와 처리수 및 요각류 위내용물에서 다양한 분류군 (규조강, 녹조 강, 남조강, 와편모조강, 은편모조강, 황갈조강)에 속하는 식물플랑크톤이 검출되었으며, 새만금호 내 시공간 차이에 따라 상이한 경향을 보였다. 현미경을 이용하여 동정한 식물플랑크톤 군집 조성의 경우 규조강이 우점한 반면, 동일한 원수의 유전자 분석 (capillary sequencing) 결과에서는 주로 녹조강, 남조강 및 와편모조강이 우점하여 다소 상반된 경향을 나타냈다. 본 연구에서 적용한 위내용물 분석에 특화된 외부 유전자 제거 전처리 방법은 농도와 처리시간 조절 등의 응용방법에 따라 다양한 동물플랑크톤 분류군에 적용이 가능할 것으로 사료된다.

국내 생태계의 환경유전자 적용도 가속화되고 있으나 생산된 데이터를 처리하고 분석하는데 한계를 느끼고 있으며, 분석 생산된 생물데이터의 신뢰성에 대한 의구심이 제기되기도 하고 시료 매체 (대상 시료, 물, 공기, 퇴적물, 위내용물, 분변 등) 간의 방법에 따른 차이와 분석 방법의 정량화와 개선 등이 필요한 상태이기도 하다. 따라서 국내 생태계의 환경 유전자를 활용한 생물다양성 연구의 신뢰성과 정확성을 확보하기 위해서는 생태분류학으로 축적된 데이터베이스를 적 극적으로 활용하여 검증 절차를 거치고, 유전자 서열 분석으로 높아진 데이터의 해상력을 전문가들이 검증하는 과정이 반드시 필요하다. 환경유전자 연구는 분자생물학적인 기술 적용으로만 해결될 수 없으며, 생산된 데이터의 신뢰성을 확보하기 위한 생태-분류-유전학-인포메틱스 등 학제 간의 연구 협력이 중요하며, 다양한 매체를 다루는 연구자들이 함께 접근할 수 있는 정보 공유 플랫폼이 절실한 분야이며, 발전의 속도도 급격하게 진행될 것이고 축적되는 데이터는 수년 내에 빅테이터로서 성장할 수 있을 것으로 기대된다.

Recently, due to the aging of workers and the weakening of the labor base in the automobile industry, research on quality inspection methods through ICT(Information and Communication Technology) convergence is being actively conducted. A lot of research has already been done on the development of an automated system for quality inspection in the manufacturing process using image processing. However, there is a limit to detecting defects occurring in the automotive sunroof sealer application process, which is the subject of this study, only by image processing using a general camera. To solve this problem, this paper proposes a system construction method that collects image information using a infrared thermal imaging camera for the sunroof sealer application process and detects possible product defects based on the SVM(Support Vector Machine) algorithm. The proposed system construction method was actually tested and applied to auto parts makers equipped with the sunroof sealer application process, and as a result, the superiority, reliability, and field applicability of the proposed method were proven.

팬픽션은 처음에는 같은 취향을 지닌 마니아층에서 무상으로 공유되었지만, 과학기술의 발전으로 인해 많은 사람이 인터넷을 통해 팬픽션을 접하게 되었다. 그중 대중들의 많은 사랑을 받은 팬픽션은 출판이 되기도 하였다. 팬픽션은 원작의 캐릭터와 인물관계를 이용해 스토리를 풀어나간다는 특징을 지니고 있어 상업적으로 출판이 되는 경우 원저작자와의 법정 다툼을 빚기도 한다. 2018년 8월 16일, 중국의 첫 팬픽션 사건에 대한 법원의 1심 판결이 진행되었다. 법원은 피고 강남에 대해 즉각 부정경쟁행위를 중단하고, 원고에 대해 손해 배상과 재고 서적을 소각할 것을 명하였다. 해당 판결은 저작권법의 보호를 받지 않는 원저작물 속 인물 요소들을 사용한 팬픽션에 대해 부정경쟁방지법으로 규제하는 것이 타당한지에 대한 논의를 촉발했다. 해당 논문은 ‘차간적소년 사건’을 통해 저작권 법의 보호를 받지 않는 원저작물속 인물 이름⋅ 인물관계 등 요소들을 사용한 팬픽션을 부정경쟁 방지법으로 규제하는 것이 타당한지에 대해 살펴 보았다. 피고는 <차간적소년>에서 원고의 원저작 물속 인물 이름, 인물관계 등 추상적인 요소만 차용하였고 구체적인 인물이나 스토리 면에서 원작과는 뚜렷한 차이점이 존재한다. 저작권법의 보호가 부여되지 않는 성과를 부정경쟁방지법의 일반 조항으로 넓게 해석하여 쉽게 확대 적용하는 것은 타당하지 않다.

The purpose of this study was to analyze the operators' perception of the allowable price range and the optimal price of kindergarten meals by using the PSM (price sensitivity measurement) technique and provide basic data on calculating kindergarten meal costs reflecting realistic meals. From April to May 2021, 779 kindergartens in Seoul were surveyed, based on 246 (31.6%) of the respondents. According to the survey, kindergarteners spent 3,506 won for meals on average, followed by 3,822 won for kindergarten attached to elementary school, 3,316 won for public kindergartens, and 2,896 won for private kindergartens (p<0.001). The allowed price range for the kindergarten meal service workers was estimated at 3,447~3854 won, 3,447 won for PMC (Point of Marginal Cheapness), and 3,854 won for PME (Point of Marginal Expensiveness). The appropriate cost of the kindergarten meal service provider was 3,950 won for kindergartens attached to elementary school, 3,425 won for public kindergartens, and 3,546 won for private kindergartens.

Recently, various researches have been studied, such as water treatment, water reuse, and seawater desalination using CDI (Capacitive deionization) technology. Also, applications like MCDI (Membrane capacitive deionization), FCDI (Flow-capacitive deionization), and hybrid CDI have been actively studied. This study tried to investigate various factors by an experiment on the TDS (Total dissolved solids) removal characteristics using MCDI module in aqueous solution. As a result of the TDS concentration of feed water from 500 to 2,000 mg/L, the MCDI cell broke through faster when the higher TDS concentration. In the case of TDS concentration according to the various flow rate, 100 mL/min was stable. In addition, there was no significant difference in the desorption efficiency according to the TDS concentration and method of backwash water used for desorption. As a result of using concentrated water for desorption, stable adsorption efficiency was shown. In the case of the MCDI module, the ions of the bulk solution which is escaped from the MCDI cell to the spacer during the desorption process are more important than the concentration of ions during desorption. Therefore, the MCDI process can get a larger amount of treated water than the CDI process. Also, prepare a plan that can be operated insensitive to the TDS concentration of backwash water for desorption.

교통카드 데이터는 도시 유동 패턴 분석을 가능하게 하는 양질의 대용량 데이터를 제공한다. 하지만 유동 클러스터링의 방법론적 어려움으로 인해 기존의 연구들은 데이터의 이점을 최대로 활용하지 못하고 있다. 이러한 한계를 극복하고자, 본 연구는 교통카드 데이터에 기반한 효율적인 유동 클러스터 탐지 기법을 제시하고, 이를 서울시 대중교통 통행 데이터에 적용하여 유의미한 유동 통행 클러스터를 도출하고자 한다. 사례 분석 결과, 서울 전체에 걸쳐 있는 다양한 대중교통 통행 클러스터를 도출할 수 있었고, 특히 공간상에서는 인접하지만 서로 다른 교통수단과 노선 하에서 이루어진 개별 유동들을 하나의 유동 통행 패턴으로 탐지할 수 있었다, 더 나아가, 일반적인 빈도분석으로는 뚜렷하게 나타나지 않지만 공간적으로 인접한 여러 다발의 유동이 모였을 때 유의미한 통행량을 가지게 되는 주요 통행 패턴을 포착할 수 있었다. 본 연구는 유동 현상을 분석하는데 중요한 방법론적 시사점을 제시하고 있으며, 제시된 알고리즘은 향후 보다 진보된 유동 클러스터링 기법을 개발하는데 필요한 기초 결과로 활용될 수 있을 것으로 기대된다.

Chitosan powder is synthesized by a deasetylation process of chitin, obtained from processing of dried shrimp shell powder. Subsequently, chitosan (CS) membranes filled by montmorillonite (MMT) particles and phosphotungstic acid are prepared, and characterized by FT-IR and SEM. The morphology, obtained by SEM for the composite membrane, showed that MMT filler is successfully incorporated and relatively well dispersed in the chitosan polymer matrix. Water and methanol uptake for the CS/MMT composite membranes decrease with increasing MMT loadings, but IEC value increases. In all prepared CS/ MMT composite membranes, the CS membrane filled by 5 wt% MMT particles exhibits the best proton conductivity, while that with 10 wt% MMT loading exhibits the lowest methanol permeability; these values are 2.67 mS·cm−1 and 3.40 × 10−7 cm2·s−1, respectively. The best membrane selectivity is shown in the CS/MMT10 composite membrane; this shows that 10 wt% filled MMT is the optimum loading to improve the performance of the chitosan composite membrane. These characteristics make the developed chitosan composite membranes a promising electrolyte for direct methanol fuel cell (DMFC) application.

Conditional spectra (CS) are applied to the seismic fragility assessment of a nuclear power plant (NPP) containment building for comparison with a relevant conventional uniform hazard response spectrum (UHRS). Three different control frequencies are considered in developing conditional spectra. The contribution of diverse magnitudes and epicentral distances is identified from deaggregation for the UHRS at a control frequency and incorporated into the conditional spectra. A total of 30 ground motion records are selected and scaled to simulate the probability distribution of each conditional spectra, respectively. A set of lumped mass stick models for the containment building are built considering nonlinear bending and shear deformation and uncertainty in modeling parameters using the Latin hypercube sampling technique. Incremental dynamic analysis is conducted for different seismic input models in order to estimate seismic fragility functions. The seismic fragility functions and high confidence of low probability of failure (HCLPF) are calculated for different seismic input models and analyzed comparatively.

The purpose of this study was to assess the forage productivity and nutritive value of kenaf at different fertilizer application amounts and various stages of maturity. The experiment was conducted from May to September 2020, the amount of 80 kg of kenaf seed/ha was supplied with different types and amounts of nitrogen fertilizer and the plants were harvested at 10-day intervals from different harvesting dates (24th August and 3rd, 13th, 23rd September). According to the different fertilizer types and application amounts, the highest kenaf height was recorded in the inorganic fertilizer amounts of 200 and 250 kg N/ha and the fresh and DM yield were significantly improved in the inorganic nitrogen amount of 250 kg N/ha. The highest CP and TDN content in the leaf was achieved in the inorganic fertilizer amounts of 150 and 200 kg N/ha, respectively; and the highest TDN content in the stem was also found in the inorganic fertilizer amount of 200 kg N/ha. According to the different harvesting dates, the highest DM ratio was found in the harvesting date of 13th September, the leaf ratio increased with advanced maturity, whereas the stem ratio decreased significantly and the highest DM yield of kenaf was recorded in the harvesting dates of 13th and 23rd September. Besides, the highest CP, CF, CA, ADF, NDF and TDN content in the leaf as influenced by different harvesting dates was 15.4, 31.8, 10.2, 22.1, 34.7 and 76.5%, respectively, and the CP, CA, ADF and TDN in stem decreased significantly with advanced maturity of kenaf. In conclusion, the optimal fertilizer amounts and the appropriate harvesting dates for a high forage yield and high-quality kenaf as livestock feed were the inorganic fertilizer application amounts of 200-250 kg N/ha and from 13th and 23rd September, respectively.

Phalaenopsis ‘KS Little Gem’ is a new cultivar with superior ornamental qualities. Newly produced cultivars are often studied to determine the optimum growing conditions, as production management practices vary within species. We focused on the effects of temperature and fertilizer application on the growth and carbohydrate content of ‘KS Little Gem’ plantlets. Two-month-old plantlets were subjected to different temperatures (20, 25, and 30°C) and fertilizer rates (0.33, 0.5, and 1 g･L-1) inside a growth chamber. Data were collected from leaves and roots regarding their growth, fresh weight, sugar content, and starch content. The results revealed that temperature significantly affected leaves and roots parameters, fresh weight, and carbohydrate content of the plantlets. However, fertilizer rates alone showed no significant effects on the growth and carbohydrate content of plantlets. The highest leaf and root growth, root fresh weight, and leaf carbohydrate content were observed in plantlets subjected to 25°C. In addition, ‘KS Little Gem’ plants grown under 30°C had significantly inhibited growth and reduced carbohydrate content in both the leaves and roots.

Lactic acid bacteria produce different types of inhibitory compound that have a bactericidal effect. The inhibitory compounds produced include metabolic end products, bacteriocins that are antimicrobial peptides with antibiotic effect, hydrogen peroxides and numerous organic acids depending on their fermentation pathway. The inhibitory activity by bacteriocins produced by lactic acid bacteria is diverse, comprising of strains capable of inhibiting different types of Gram-positive microorganisms to those that affect only organism of close general. With the growing concern over the spread of antibiotic-resistant microorganisms and the possibility of current therapies becoming inefficient, it is imperative to find antibiotics alternatives. In food preservation, naturally occurring peptides with antimicrobial activity are favoured over chemical preservatives a more reason why there should be a holistic approach to taking advantage of bacteriocinogenic bacteria in medicine and food industry. This review focuses on the application of bacteriocin in food preservation, food industry, livestock and medicine.

Eruca sativa (E. sativa)는 루꼴라라고 불리우고 이탈리아 요리에 많이 사용되는 식물로 Brassicaceae 속에 속하는 다년생 식물이다. E. sativa를 70% 에탄올로 환류추출 및 농축하고 (ES), n-hexane (EHex), ethyl acetate (EEA), chloroform (ECHCl3), n-butyl alcohol (EBuOH) 및 water (EDW) 용매 극성별로 분획하여 미백 효능을 규명하고 화장품 소재로서의 응용성을 연구하였다. ES, EHex, EEA, ECHCl3, EBuOH 및 EDW의 mushroom tyrosinase 활성 저해를 평가한 결과 EEA가 가장 유의하게 저해하였으며, B16F10 멜라노마 세포 내에서의 멜라닌 생성을 확인한 결과 ES, EEA 및 EBuOH 를 처리하였을 때 멜라닌 생성이 유의하게 저해되었으며, 특히 EEA 분획물에서 가장 효과가 뛰어났다. 피부염과 여드름을 일으키는 황색포도상구균, 여드름 원인균을 포함한 8종의 세균, 진균에 대해 ES, EHex, EEA, ECHCl3, EBuOH 및 EDW의 항균력과 방부력 시험을 평가한 결과 EEA는 8종의 균 모두 효과적으로 항균력을 나타냈다. ES를 0.05, 0.1%를 함유하여 제조한 토너와 에멀션은 기존 처방에서 방부력을 증가 시켜 천연보존제 역할을 할 수 있을 것으로 판단된다. ES를 토너, 에멀션 제형에 0.05, 0.1%로 첨가하여 일광, -20, 4, 25, 55 ℃ 조건에서 4 개월동안 안정성을 평가한 결과 pH 및 점도에 대한 영향은 크게 없었 으나 대조군 및 ES를 첨가한 에멀션 55 ℃에서 분리가 일어나는 것을 확인하였다. 또한, 피부침투시험을 통해 EEA는 6 hr에서 0.058%가 침투되어 임상적인 효능을 예측할 수 있었다. 천연소재 E. sativa는 미백 기능성화장품 소재 및 천연 항균 보존제로 활용 가치가 있을 것으로 사료된다.

연구목적은 코로나-19 확산 속에서 비대면 수업의 적용에 따른 보건계열 학과 1, 2 학년 학생들의 전공 몰입과 전공 만족도의 변화를 분석하는 데 있다. 연구대상은 보건계열학과에 재학 중인 1, 2학년 학생으로 총 181명이 참여하였다. 대상자들은 COVID-19 확산에 따라, 15주간 비대면 수업에 참여하였으며, 비대면 수업은 교육부 지침에 근거하여, 실시간 온라인 강의와 영상콘텐츠를 활용한 수업으로 구성되었다. 전공 몰입 평가는 전공몰입척도를 활용하여 수행되었고, 전공 만족도는 전공만족도척도를 활용하여 수행되었다. 분석결과, 비대면 수업의 적용 후 대상자들의 전공 몰입과 전공 만족 정도가 통계적으로 유의미하게 감소되었다. 본 연구결과에 따라, 비대면 수업의 적용은 대상자들의 전공 몰입과 전공 만족도의 감소에 영향을 미칠 수 있는 요인이다. 따라서, 비대면 수업의 적용에서 보건계열 학과 1, 2학년 학생들의 전공 몰입과 전공 만족도의 향상을 위해서, 적절한 고려가 필요할 것이다.