Three-week old Commelina communis was transferred and grown in Hoagland solution containing 100μM Cd2+, 100μM Cd2++100μM kinetin, 100μM Cd2++100μM zeatin and 100μM Cd2++200μM zeatin for 7 days, and then a number of physiological activities were investigated. In control, the length of the stem of plants was increased to 4.7cm, but in Cd2+, Cd2++kinetin, Cd2++100μM zeatin and Cd2++200μM zeatin treatments, the growth of plants were increased to 1.5cm, 2.1cm, 3.9cm and 4.3cm, respectively. In the treatments of Cd2+, Cd2+ +kinetin, Cd2++100μM zeatin and Cd2++200μM zeatin, total chlorophyll contents were reduced to 26%, 24%, 15% and 3%, respectively, on the contrast to the control. In chlorophyll fluorescence experiments, Fv/Fm ratios were also reduced to 44%, 21%, 17% and 5% in the light intensity of 2100μmole E m-2s-1 by Cd2+, Cd2++ kinetin, Cd2++100μM zeatin and Cd2++200μM zeatin treatments on the contrast to the control. Water stresses were increased to 2.6, 1.7 and 1.2 times by Cd2+, Cd2++kinetin and Cd2++100μM zeatin. On the other hand, combination of Cd2++200μM zeatin reduced water stress to 0.12%. In Cd2+ accumulation experiments Cd2+ transports were inhibited to 33%, 48% and 70% by Cd2++kinetin, Cd2++100μM zeatin and Cd2++200μM zeatin. Therefore, it could be concluded that zeatin clearly reduced the toxicities of Cd2+ by reducing the absorption of Cd2+.
감마선(0, 0.5, 1, 2, 3 kGy) 조사와 methyl bromide(MeBr, 2 g/kg, 4 hr) 훈증 및 이들의 처리시기가 사과의 생리화학적 품질특성에 미치는 영향을 비교하여, 검역처리를 위한 감마선 조사의 응용성을 검토하였다. 감마선과 MeBr 처리에 따른 사과의 생리화학적 품질평가에서 2 kGy 이상의 감마선 조사와 MeBr 훈증은 사과의 일부 생리화학적 품질특성의 변화를 촉진시키는 것으로 나타났다. 감마선과 MeBr의 처리
본 시험에서는 원형 포트에 재배한 맥류(피맥 2품종, 과맥 2품종)의 염(NaCl, MgSO4 )을 도별(0, 60, 120, 180mM)로 출수기에 10일 동안 처리하여 출수기의 내염성 정도와 이에 수반된 몇 가지 대사물질의 변화와 수량구성요소의 변이를 조사하여 재배법개선, 그리고 내염성이 큰 품종육성을 위한 기초자료를 얻고자 수행하였다. 염류간의 비교에서 수수, 간장, 일수립수, 그리고 수량에 있어서는 유의성이 없었으며 건경중과 천립중에서는 염종류간 차이가 있어서 유의성이 인정되었는데 MgSO4 보다 NaCl에서 높았다. 지엽의 조사에서는 엽록소와 유리 proline이 유의성이 있었는데, 엽록소는 NaCl 보다 MgSO4 에서 높았으나 유리 proline 축적은 MgSO4 보다 NaCl에서 높았다. 농도간 비교에서는 모든 조사항목이 유의성이 있어서 농도간의 차이가 나타났는데 중실수량을 포함한 모든 항목이 전반적으로 저농도에서 높고 고농도일수록 낮았다. 지엽의 조사항목에서도 모두 유의성이 인정되었는데 농도증가에 따라 생건비는 증가하는 경향이지만 상대팽압도와 엽록소는 상당히 감소하는 경향이었다. 그리고 유리 proline 함량은 대조적으로 농도증가에 따라 축적량이 증가되었는데 대조에 비하여 많으면 10배까지 증가되었다. 종합적으로 볼 때 생식생장기의 생육단계에서 유리 proline축적이 많으며 수량구성요소와 종실수량의 감소가 적은 백동이나 동보리1호가 상대적으로 내염성이 높게 나타났으나, 출수기의 염 stress는 생육장해가 커 수량감소를 초래하므로 이시기의 관리가 중요하다고 하겠다포장 출수기, 성숙기는 결빙방지 단백질의 농도, 광합성의 광저해에 대한 내성, 포장 내동성과 고도의 정의 상관을, 등숙기간은 각 형질들과 부의 상관을 보였다. was "glossy and smooth" in all cases and preference about the texture was high. The Doenjang with added P. japonica Powder had a saltier taste and the Doenjang with P. japonica Powder had the least sweet taste. In the flavor and overall Preference, the Doenjang with P. japonica powder was the lowestEX>로 측정되었고, 계사내 지붕의 표면 온도는 최고 29℃ 가 측정되었다. 계사 내 표면 온도 및 닭의 표면 온도는 계사내 공기온도의 영향을 많이 받는 것으로 나타났다.ill in a good agreement with those predicted by Rohsenow's formula, which was based on nucleate boiling. For the condenser, the wall temperatures were practically uniform, and the measured values of condensation heat transfer coefficient were 1.7 times higher than the predicted values obtained from Nusselt's film condensation theory on tilted
This study was conducted to evaluate the drought tolerance of Japonica and Indica rice cultivars during germinating and seedling stages by using the polyethylene glycol (PEG) solution. Each 5 cultivars of Japonica and Indica were cultured from 14 days after seeding(DAS) to 21 DAS using the PEG solution in a moderate water potential (-0.63 MPa). The lengths of radicle and plumule during the germinating stage were inhibitied by the PEG treatment to about 50% and 85%, respectively. The application of PEG to the seedling of two rice types caused to inhibit the plant height and leaf age about 23 % and 10%, respectively. Shoot and root dry weights by PEG treatment were inhibited more severely in Japonica than those in Indica. The difference on delaying of leaf area expansion between both rice types was not found with treatment of PEG, while the leaf color was increased in both Japonica and Indica by 19.9% and 9.2%, respectively. The average photosynthetic ability was inhibited more in Japonica to 36.0% than did Indica to 27.9%. The stomatal conductance was severely affected by PEG treatment, and the degree was varied in both rice types, ranged with 80-85% in Japonica and 29.3-81.6% in Indica. These results indicate there is little relationship between seed germination and seedling growth under the stress of low water potential.
The object of this study was to determine the difference of the time course changes of transpiration, diffusion resistance and photosynthetic rate of rice at several different growth stages subjected to soil moisture stress (SMS) and recovery by irrigation. A japonica rice cultivar 'Dongjinbyeo', was grown under flooded condition in a plastic container filled with silty loam soil. At 5 main growth stages, the container was treated by SMS until initial wilting point (IWP) and then reirrigated. The duration of SMS until IWP were the longest, 13 days for tillering stage, and the shortest, 7 days for panicle initiation and meiosis stage. The transpiration rate rapidly decreased during SMS and the transpiration rate at IWP of the stressed plant showed 10∼20% compared with control, and the transpiration rate of stressed plant at most growth stages also recovered rapidly after irrigation and then reached 100% of control within a week. The shoot photosynthetic rate in all growth stages rapidly decreased by SMS, and the rates at IWP of stressed plants were de-creased nearly to 0%, beside the treatment at tillering stage. The recovery degree of photosynthetic rate by irrigation ranged from 20 to 90%, showed higher at early growth stages of SMS treatment than that of later stages. At all growth stages the leaf diffusion resistance of stressed plants was over 3 times that of the control resulting from a rapid increase at 3 to 5 days after draining for SMS, and showed quick recovery by irrigation within 3 days after drainage. The above physiological parameters changed in close relation with the decrease of the soil matric potential after SMS. These results indicate that at all main growth stages of rice plants the transpiration and photosynthesis reduction by stomatal closure reponded sensitively to the first stage of SMS closely related with decrease of soil water potential, while those recovery pattern and recovered degree by irrigation are little different by growth stage of rice.
Methyl methanesulfonate (MMS) was fed to Drosophila melanogaster in order to investigate its toxic capability at developmental and adult stages, and the hereditary effect of toxicity and the potency for induction of sex-linked lethal mutation during the spermatogenesis by the means of an attached-X method.
In the control group, the egg to adult viability of D. melanogaster was 95.2%, while 3.5mM and 5.0mM treated groups were 90.0% and 84.1%, respectively. In the case of their progenies (F_1), the viability was 96.9% in the control group, while 3.5mM and 5.0mM treated groups were 54.5% and 1.6%, respectively. Therefore, these differences between two generations show significant physiological toxic effects in the next generation. In the parental generation, the developmental time was calculated 11.05 days in the control group, 12.43 days in 3.5%mM treated group, and 13.23 days in 5.0mM. In the case of F_1 it was estimated 10.35 days in the control group, and 11.43 days in 3.5mM treated group. Compared with the control groups in two generations, the developmental time generally delayed as the dose of MMS increased. As to the sex-ratio, there was no differences between the control and MMS treated groups. The toxic values of adult stage showed which increased the frequency of mortality with MMS concentrations. The mortality at 120hr in the control group was 1.67% and it in 0.5mM MMS treated group 3.33%. In 2.5mM MMS treated group, it was 33.3% at 72hr, and it 95% at 120hr. The increase of the morality was shown from 72hr in 4.0mM treated group which was 100% at 96hr. There was the concentration-dependent induction of sex-linked lethal mutation during the spermatogenesis by means of an attached-X method, MMS had more pronounced effect in sperm and spermaid stages in D. melanogaster.
전보 10)의 경과 품종, 종자숙도의 차이에 따라 중배축 신장성 정도가 다르게 나타난 것이 유전 또는 생리적 특성의 차에 기인한 것인지를 구명하기 위해 수행하였던 바 실험결과를 요약하면 다음과 같다. 1. 습윤.고온처리에 의한 중배축 신장 효과는 처리구가 무처리구보다도 7배 이상 크게 신장하여 그 효과가 현저하였다. 2. 토양수분 함량에 따른 중배신장은 수분함량 8%구에서 최고치를, 16%구에서 최저치를 나타내 토양수분함량이 높을수록 작아지는 경향이었다. 3. 식물 Hormone중 중배축신장 효과는 cis-ABA 처리구가 GA3 , IAA 및 Kinetin 처리구보다도 그 효과가 현저하게 컸다. 4. 중배신장과 분리근장과는 어떠 뚜렷한 경향을 찾을 수 없었으나 중배축장과 분리근의 건물중간에는 중배축장이 클수록 건근중이 크게 나타났다.
수도의 직파재배에 있어서 가장 취약점인 출아.입묘율을 향상시키는데 많은 영향을 미치는 중배축 신장에 대하여 검토하였던 바 그 결과를 요약하면 다음과 같다. 1. 품종의 기원별 중배축장은 다수형 품종이 9.6mm로 재래종과 일본형 각각 4.4, 3.2mm 보다도 훨씬 컸다. 2. 생태형별 중배축 신장은 tjereh, aman, aus, boro 및 bulu 순으로 tjereh가 29.3mm로 buld의 5.4mm에 비해 약 5배나 컸다. 3. 품종간 중배축장은 재래종에서 백경조, 다다조 및 해조가, 일본형은 상풍벼, 팔달 및 간량도가, 다수형 품종에서는 원풍벼, 가야벼, 밀양30호 및 수정벼가 컸다. 4. 종자 숙도별 중배축장은 개화 1주후 채종 종자가 39.3mm로 가장 컸고, 성숙일수가 길어짐에 따라 중배축장은 점차 작아졌다. 5. 종자 저장기간이 길어질수록 종자숙도와 관계없이 중배축장은 점차 감소하는 경향이었고, 저장온도 5℃ 구가 15℃ , 25℃ 에 비하여 중배축장의 감소정도가 작았다.
통일형품종과 일본형품종간의 침관수저항성 차이를 명확히 하고자 유묘기, 분얼성기 및 수잉기에 각각 관수처리하여 관수저항성과 관계한 생리 화학적 특성을 검토한 결과는 다음과 같다. 1. 유묘기 관수처리로 묘생존율은 'FR13A'가 91.8%, 통일형품종들이 33.8~40% 인데 비하여 일본형품종들은 완전 고사하였으며 묘생존율이 높은 품종 일수록 관수중 O2 발생량이 높았고 POD활성은 낮았으며 Catalase 활성은 높은 경향이었다. 2. 분얼성기 관수처리로 일본형품종들은 통일형품종들 보다 관수기간중 초장 이상 신장이 현저하였고 관수후 초장회복이 느렸으며 관수일수가 증가됨에 따라 수량 감소도 급격하였다. 3. 수잉기 관수처리로 통일형품종들은 일본형품들 보다 광합성, 암호흡, 근산화력이 높았고 에틸렌생성량은 낮은 특성을 보였으며 감수율도 낮았다. 감수율도 낮았다.