Multiple interferon tau (IFNT) genes exist in bovine. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), one of new type I interferon tau (IFNT) genes. The objectives of this research were to investigate whether multiple, distinct gene encode bIFNT1 and other type I bIFNT gene in the bovine genome or not and to examine the expression of bIFNT1 and other bIFNTc1 mRNAs during conceptus development. The transcription of these genes could be regulated through caudal-related homeobox-2 (CDX2) and ETS2 and/or AP1(JUN) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. The presence of mRNAs encoded by bIFNT1 and type I bIFNTc1 genes were examined quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis of total cellular RNA (tcRNA) extracted from on the days 17, 20 and 22 bovine conceptuses. bIFNT1 was highly expressed on the day 17 and transcripts were gradually and weakly detectable on the days 20 and 22. However, the other bIFNTc1 gene examined transcripts was highly expressed on the day 20 and transcripts were weakly detectable on the days 17 and 22 bovine conceptuses. Furthermore, human choriocarcinoma JEG3 was co-transfected with an -1kb-bIFNT1/c1-Luc constructs and several transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1-Luc increased when this constructs were co-transfected with, ETS2, AP1(JUN), CREBBP and/or CDX2. Also, bIFNTc1 gene was had higher effect on activity by alone ETS2, and AP1(JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNT1 gene expression of the upstream region was not idented. These results demonstrate that these genes display differential, tissue-specific expression and developmental regulation during pregnancy.

Kisspeptin-10 (KP-10) has been reported to act as a tumor metastasis suppressor via its receptor, G protein-coupled receptor 54 (GRP54). The KP-10/GPR54/BMPs signaling pathway plays an important role in embryonic kidney development. However, its function in osteoblast differentiation is unknown. The aim of this study was to confirm the molecular mechanism for the action of KP-10 on osteoblast differentiation. Expression of the Bone morphogenetic protein-2 (BMP2) and osteogenic genes were determined by RT-PCR and real-time PCR analysis in C3H10T1/2 cells. Transient transfection assays were performed to confirm the effects of KP-10 on BMP2-Luc activity. BMP2 and phospho-Smad1/5/9 protein levels were determined by Western blot analysis. Alkaline phosphatase (ALP) staining experiment was performed to evaluate ALP activity. To further confirm the effect of KP-10-induced GPR54, we used GPR54 Knock out (KO) C3H10T1/2 cells. KP-10 significantly increased osteogenic gene such as Runx2, ALP and Dlx5 in C3H10T1/2 cells. The ALP staining levels were also increased by KP-10. Interestingly, BMP2 mRNA, protein expression and promoter activity were also increased by KP-10. However, KP-10-induced BMP2 expressions were not increased in GPR54 KO cells. These results suggest that KP-10 increases BMP2 expression through GPR54. Next, Western blot analysis shown Smad1/5/9 phosphorylation were enhanced in a time-dependent manner by KP-10 treatment. It is well known that BMP2 increased phosphorylation of Smad1/5/9 via BMP2 receptor. In addition, KP-10 increased NFATc4 mRNA levels and NFATc4 overexpression enhance BMP2 mRNA levels. To confirm the KP-10-induced BMP2 action, we used KP-10-treated medium in wild type cells and GPR54 KO cells. The osteogenic genes were not elevated by KP-10-treated medium (GPR54 KO cells) whereas increased expression levels by KP-10 medium (wild type cells). These data indicate that KP-10 induced osteoblast differentiation through NFATc4-mediated BMP2 signaling.

The effects of some essential oils and Sopophora extract on Frankliniella occidentalis adults, Myzus persicae adults, Plutella xylostella larvae, and Spodoptera exigua larvae were investigated. Insecticidal and phytotoxicity activities of emulsion in water formulations containing the active essential oils as active ingredients under laboratory and pot conditions were also tested. Based on these results, NRS-13 and NRS-24 formulations were selected to further assess as follows; the GC and GC-MS analysis on the active essential oils, the quantity analysis of the major components contained in the formulations, their insecticidal activities under pot and field conditions, and their oral, dermal, skin and eye irritation, and fish toxicities. Thus, the NRS-13 (BaechooSaferTM) and NRS-24 (JindiOutTM) formulations could be considered as insecticides for pest control.

The American house dust mite, Dermatophagoides farinae Hughes, 1961 (Acari: Pyroglyphidae), is recognized as an important source of allergens in the domestic environment. An assessment was made of the toxicity of constituents from essential oil of cade, Juniperus oxycedrus L. (Cupressaceae), and related compounds as well as four experimental spray formulations containing the oil (10–40 g/liter sprays) to adult D. farinae. In a contact + fumigant mortality bioassay, methyleugenol (24-h LC50,5.82 μg/cm2) and guaiacol (8.24 μg/cm2) were the most toxic constituents to the mites and the toxicity of these constituents and benzyl benzoate did not differ significantly.

We evaluated the repellent activity of 12 Apiaceae plant essential oils and their components against male and female adult German cockroaches, Blattella germanica, to find new natural repellents. Of the plant essential oils tested, ajowan (Trachyspermum ammi) and dill (Anethum graveolens) essential oils showed the most potent repellent activity against male and female adult German cockroaches. Of the compounds identified, carvacrol, thymol, and R-(-)-carvone showed >80% repellent activity against male and female adult German cockroaches at 0.2 mg/L air concentration. S-(+)-Carvone, (+)-dihydrocarvone, and terpinen-4-ol showed >70% repellent activity against male and female adult German cockroaches at 0.8 mg/L air concentration. Our results indicated that Apiaceae plant essential oils and their constituent have good potential as natural repellents against adult German cockroaches.

We evaluated the larvicidal activity of 12 Apiaceae plant essential oils and their components against the Asian tiger mosquito, Aedes albopictus, and the inhibition of acetylcholine esterase with their components. Of the 12 plant essential oils tested, ajowan (Trachyspermum ammi), caraway seed (Carum carvi), carrot seed (Daucus carota), celery (Apium graveolens), cumin (Cuminum cyminum), dill (Anethum graveolens), and parsley (Petroselinum sativum) resulted in >90% larval mortality when used at 0.1 mg/mL. Of the compounds identified, α-phellandrene, α-terpinene, p-cymene, (-)-limonene, (+)-limonene, γ-terpinene, cuminaldehyde, neral, (S)-+-carvone, trans-anethole, thymol, carvacrol, myristicin, apiol, and carotol resulted in >80% larval mortality when used at 0.1 mg/mL.

Agricultural by-productsis a rich source of dietary fibers including arabinoxylans, which is found mainly in water-insoluble form and reported to have immunomodulatory activities. We investiglatedwhether solublearabinoxylan (AX)-enriched byproducts alters macrophage activity.Agricultural by-products wereorally given to mice for 4 weeks. Peritoneal macrophages from mice were assayed for scavenger receptor expression, phagocytosis, and lipopolysaccharide (LPS)-stimulated responses. Serum levels of inflammatory cytokines were determined 1 hour after intraperitoneal injection of LPS. Agricultural by-productstreated mice showed a higher expression of SRA and CD36, accompanied by enhanced phagocytosis of latex beads, relative to control mice. Upon stimulation with LPS ex vivo, macrophages from the byproducts group showed lower levels of CD86 and CD40 at the surface protein level and lower production of NO, tumor necrosis factor (TNF)-α, and interleukin (IL)-6, compared with the control group. Theagricultural by-products group showed a decrease in serum levels of LPS-induced TNF-αand IL-6, while mice given crude byproducts, which was prepared without enzymatic hydrolysis, showed a reduction only in serum TNF-α,indicating that soluble arabinoxylans contributes to the immunomodulatory effect ofagricultural by-products. Our data present evidence that soluble arabinoxylan-enriched agricultural by-products can be an immune enhancing functional food.

The antioxidant activity of 70% methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts from turmeric (Curcuma longa L.) was investigated in this study. The antioxidant potential of various extracts of turmeric was evaluated by using different antioxidant tests, namely DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, ABTS [2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, ferric reducing antioxidant power and β-carotene bleaching assays as well as total phenol content and total flavonoid content. All extracts exhibited abundant total phenol content, total flavonoid content and strong antioxidant activity. Particularly, higher activity was exhibited by CM extract with 46.32±0.35 mg CAE/g and 15.58±0.39 mg QE/g of total phenol content and total flavonoid content. Generally, antioxidant activity of various extracts from turmeric increased in the following order: 70% methanol extract, 70% ethanol extract and CM extract, respectively. Results suggested that turmeric could be widely used as a source of antioxidant and a kind of functional material in food.

Fucoidan is a sulfated polysaccharide that is purified from brown algae, such as Fucus vesiculosus. This compound has multiple biological activities including immune-stimulating, and anti-viral activities. We recently demonstrated that the cytotoxicity of fucoidan can be dependent on the batch of its production and its molecular weight. In a previous study, fucoidan B exerted cytotoxicity toward mouse spleen cells. To confirm the biological activity of Fucoidan B, we cultured HL-60 cells, a human leukemia cell line, and treated then with fucoidan. The metabolic activity of the HL-60 cells decreased in response to treatment by fucoidan. Moreover, the morphology of HL-60 treated by fucoidan changed. To investigate the fucoidan’s effects, we analyzed the size and level of Annexin V/propidium iodide staining of HL-60 cells using a flow cytometer. Fucoidan consistently induced cell death, including apoptosis of HL-60 cells. As potential mechanisms, fucoidan destabilized the mitochondrial membrane potential and altered the production of reactive oxygen species in HL-60 cells. Taken together, these results suggest that fucoidan has anti-tumor activity on HL-60 cells via destabilization of mitochondrial membrane potential. The present study demonstrates that fucoidan can be used as an anti-cancer agent for leukemia.

This study was conducted to investigate changes in the proximate composition, antioxidant activities, and α-glucosidase inhibitory activity of Opuntia ficus-indica (OFI) cladodes cultivated in Jeju (JJ1, JJ2, JJ3) and Jeonnam (JN1, JN2). The difference in the proximate composition (crude protein, lipid and ash content) of OFI between the two regions was not significant. Ca, Mg and Na were the major mineral components of OFI. The ascorbic acid content of OFI ranged from 57.87 to 143.72 mg/100 g. A 70% ethanol extract was used to investigate the antioxidant content and activity as well as the α-glucosidase inhibitory activity. The total polyphenol and flavonoid contents of OFI were 38.69~55.29 and 3.33~4.03 mg/g, respectively. The antioxidant activities based on the DPPH and ABTS free radical scavenging assays were 45.19~ 61.52% and 39.15~51.96%, respectively, at a concentration of 1 mg/mL. The inhibitory activity of OFI extracts against rat intestinal α-glucosidase was 29.72~45.73% at 1 mg/mL concentration, and JN1 showed the highest α-glucosidase inhibitory activity. This information could be very useful for authentication of Opuntia species with the highest potential as sources of nutritional and therapeutic elements.

The black raspberry (Rubus coreanus Miquel) contains anthocyanin, tannins, gallotannin, gallic acid, ferulic acid and phenolics. It brightens the eyes and protects the liver and kidneys. It was effective for anti-aging. Thus, the purpose of this study was to inform the excellence of black raspberry and to screen antioxidant activity to ensure the possibility as a functional material. In this study, bioactive compounds in black raspberry were determined. Additionally, black raspberry was extracted by CM (chloroform:methanol, 2:1, v/v), 70% methanol and 70% ethanol, and were investigated and compared in vitro methods. Total phenol and flavonoid contents were measured to compare each different solvents. In addition, DPPH radical scavenging activity and ABTS radical scavenging activity, ferric reducing antioxidant power, reducing power were determined to measure the antioxidant activity. The results were the highest in 70% ethanol extracts, and the higher the concentration showed a significantly higher antioxidant capacity. When observed the relationship between the study, antioxidant activity of black raspberry was supposed to affect by the anthocyanin, phenol and flavonoid contents.

The study examined qualities including chlorophyll, total phenol, total flavonoid content and antioxidative activity in 7-year-old mistletoe that was artificially cultivated with Japanese apricots as the host. The chlorophyll a content of the artificially cultivated Japanese apricot mistletoe leaves was 7.67 mg/g (old leaves) and 3.17 mg/g (new leaves), while the chlorophyll b content was 0.80 ~ 3.53 mg/g and 1.31 ~ 3.56 mg/g. The carotenoid content of the Japanese apricot mistletoe leaves were in the range of 0.29 ~ 2.48 mg/g, and the old leaves (1.85 mg/g) had a carotenoid content 2.1 times higher than the new leaves (0.89 mg/g). The total polyphenol content of the leaves and nodes of the 7-year-old mistletoe was 60.22 mg/100 g TAE for the leaves and 35.50 mg/100 g TAE for the nodes. Among the host trees, polyphenol content was highest (81.44 mg/100 g TAE) in the old mistletoe leaves of the Quercus mongolica, while the total flavonoid content of the Japanese apricot mistletoe was higher in the new leaves (47.89 mg/100 g RUE) than the old leaves (42.19 mg/100 g RUE). The DPPH radical scavenging ability of the Japanese apricot mistletoe scored a low 22.4 ~ 28.5% in the leaves, but was relatively high in the nodes at 52.4 ~ 80.1%. In terms of 1mg/mL concentration, the new leaves (69.1%) and old leaves (82.3%) of the Quercus monglica mistletoe displayed the highest inhibition rate. Chestnut mistletoe displayed an inhibition rate of 21.9 ~ 31.9% for the leaves, and 35.0 ~ 63.8% for the nodes. These results indicate that Japanese apricot mistletoe would be substitute oak tree mistletoe as a source of bio-active property for functional food and medicine.

This study was conducted to investigate the total polyphenol, total flavonoid and the antioxidant activity in different origin and parts of Moringa olerifera cultivated in Cheorwon. The extraction yield from leaf, root and stem were 25.5, 23.0, 12.3%, respectively. The total polyphenol content was high in the leaf extracts (15.77 mg/g) followed by root extracts (11.23 mg/g) and stem extracts (3.50 mg/g) but the difference of origin was not observed. The total flavonoid content was high in the leaf extracts (14.78 mg/g) followed by stem (1.36 mg/g) and root (1.14 mg/g). The total flavonoid content of Philippines and Thailand leaf were statistically high compared to india leaf. The DPPH radical scavenging activity was high in the leaf extracts at the 1 mg/ml, 0.25 mg/ml but the difference of origin was not observed. The DPPH radical scavenging activity may be attributed to the presence of several compounds such as polyphenol and flavonoid. Hence consumption of diet supplemented with Moringa olerifera leaves could protect the human against diseases induced by oxidative stress.

This study was conducted to get beneficial information of fatty acid compositions and antioxidant resources from safflower genetic resources. The fatty acid compositions and antioxidant activities in 128 safflower germplasm collected from South Asia and Africa were evaluated using gas chromatography and spectrophotometer, respectively. The total oil contents in safflower germplasm were 15.8 ~ 32.2%. The unsaturated fatty acid and linoleic acid were 89.4 ~ 93.3%, and 15.5 ~ 80.4%, respectively. Six accessions (K184652, K184658, K184662, K184671, K185831, and K185832) from South Asia exhibited high oleic acid contents. Variation of DPPH and ABTS activities ranged from 0.8 ± 0.28 to 7.6 ± 0.06 μg ASC mg-1 and from 23.1 ± 1.72 to 134.7 ± 1.25 μg Trolox mg-1, respectively. Total polyphenol contents ranged from 5.1 ± 0.17 to 52.0 ± 1.24 μg GAE mg-1. The antioxidant activities and fatty acid compositions were not different between the South Asia and Africa germplasm, while oil contents of seeds were higher in South Asia germplasm. DPPH values had positive correlation with ABTS activity (r = 0.862**) and total polyphenol contents (r = 0.864**) whereas oleic and linoleic acid showed strong negative correlation (r = −0.998**). Principal component analysis based on oil and antioxidant traits exhibited that the first four principal components together expained 85.0% of the total variation.

Background: Many previous studies recommended the side-lying hip abduction (SHA) exercise for targeting the gluteus medius (Gmed) and gluteus maximus (Gmax) muscle activity while the decreasing tensor fasciae latae (TFL) activation. Mischoice of hip position and angle in SHA may increase the risk of lower extremity injuries and undesirable muscle activation. However, information is limited on the effect of composite hip flexion angles and hip rotation on the gluteal muscle activity during SHA. Objects: This study aimed to compare muscle activity (Gmed, TFL, and Gmax) and activity ratios (Gmed/TFL, Gmax/TFL, and Gmed/Gmax) using surface electromyography (EMG) during SHA exercise at three different hip flexion angles either with or without internal rotation (IR) in subjects with Gmed weakness. We hypothesized that applying hip flexion and IR during SHA would increase gluteal muscle activity and decrease TFL activity. Methods: Muscle activity and activity ratios in 20 volunteers with Gmed weakness during 6 different SHA were investigated with surface EMG. One-way repeated-measures analysis of variance was used to determine the statistical significance. Results: Significant differences were found among the six different exercises for Gmed (F2,41=11.817, p<.001) and Gmax (F3,52=5.513, p=.003) muscle activity, and Gmed/TFL (F3,54=8.735, p<.001) and Gmax/TFL (F2,37=4.019, p=.028) activity ratios. Conclusion: Applying hip flexion is an effective method for increasing gluteal activity, and it elicits great Gmed/TFL and Gmax/TFL activity ratios during SHA in subjects with Gmed weakness.

Background: Recently, there has been an emphasis on the use of interventions with biofeedback information for the maintenance or correction of posture. Objects: This study assessed the change of trunk posture and trunk muscle activation when people exhibiting postural kyphosis performed visual display terminal work with or without a contact feedback device (CFD). Methods: Eighteen right-handed individuals were recruited. Thoracic angle and right thoracic erector spinae (TES) muscle amplitude were analyzed. There were two sessions in these experiments. The control session involved 16 minutes of typing without a CFD, and the CFD session involved 16 minutes of typing with a CFD. The visual analog scale score was analyzed with a paired t-test, and the kinematic and electromyography data were analyzed through two-way repeated analysis of variance. Results: The paired t-tests revealed that subjects had significantly less pain after the CFD sessions than after the control sessions (p<.05). Significant main effects by session and by time were observed in the thoracic kyphosis angle (p<.05). There was a significant session×time interaction for TES amplitude (p<.05), along with significant main effects by session and by time (p<.05). Conclusion: The CFD caused people with postural kyphosis to straighten and to activate their TES continuously, even though they were habituated to bend their bodies forward. Therefore, the CFD was a beneficial treatment tool

The effect of drying temperature and steaming time on the browning and antioxidant activity of dried Platycodon grandiflorum was investigated. Thirteen treatment conditions were constructed using central composite face-centered design containing 5 center points. Drying temperature and steaming time (as factors) were 45-75oC and 15-45 min. According to treatment conditions, dried Platycodon grandiflorum was assessed for color characteristic, degree of browning, total polyphenol content, and DPPH and ABTS free radical scavenging (as responses). When increasing drying temperature within a given steaming time, dried Platycodon grandiflorum exhibited decreased lightness, increased redness, degree of browning, and total polyphenol contents, and enhanced antioxidant activities. Except for total polyphenol contents and antioxidant activities, steaming time within a given drying temperature exhibited similar effects to those observed in drying temperature. However, steaming time did not likely influence total polyphenol contents and revealed the opposite trends observed for the effect of drying temperature on their antioxidant activities. The overall results suggested that drying temperature was the main factor for changes in the browning and antioxidant activity of dried Platycodon grandiflorum.

본 연구는 잠재적인 생균제제 Lactobacillus plantarum P1201을 이용하여 갈색콩 두유의 젖산발효 중 이화학적 특성, 총 phenolics, isoflavone 함량 및 항산화 활성 변화를 연구하였다. 갈색콩 두유는 발효가 진행됨에 따라 pH가 최종 4.03으로 감소하였으며, 이에 따라 총산이 증가하여 발효 후(60시간) 1.03%를 나타내었다. 생균수 역시 11.55log cfu/g으로 발효 과정 중 점차적으로 증가하였다. 총 phenolics 함량은 발효 전 2.87mg/g에서 발효 후 2.98mg/g으로 약간 증가하였다. Isoflavoneglycosides 및 -malonylglycosides 형태는 감소하였고 -aglycones 형태는 증가하였다. 특히 발효 초 기 isoflavone-aglycones 함량은 38.30μg/g에서 증가하여 발효 36시간째 84.31μg/g으로 최대치를 나 타내었고 이후에는 감소하였다. 발효 36시간째 isoflavone-aglycones 형태인 daidzein, glycitein 및 genistein의 함량은 각각 24.12μg/g, 25.25μg/g 및 24.71μg/g이었다. 한편 DPPH 및 ABTS 라디칼 소 거활성 및 환원력은 발효 중 서서히 증가하였다.