This study is on the early historical background and the process of development of modern architecture in Thai, during the period from 1782(King Rama 1) to 1934(King Rama 7). Thai started tn form a connection with western nations from the late 18C, founded the Bangkok Dynasty. Since then, Thai was increasingly influenced by western civilization and this trend included an increasing influence of western architecture. In this paper, the centeral objective is to observe the architectural change during the period from King Rama 1(1782-1809) to King Rama 7(1925-1934). This can be divided into three period. During the first period, from King Rama 1 to King Rama 3(1824-1851), Thai architecture showed a tendency to follow the preceding traditional example and to imitate Chinese architecture. The second period is from King Rama 4(1851-1868) to King Rama 5(1868-1910). During this period, Thai architectural design was increasingly influenced by western concepts, specially European Neo-classicism Style. During the third period, from King Rama 6(1910-1925) to King Rama 7(1925-1934), Thu had been enjoying extensive commerce with western nations. The great developments in the field of architecture during this reign were apparent in the construction of public utilities and facilities. These buildings were designed by western architects. At this time, modern architectural concept of western was introduced.

This experiment was carried out to evaluate the effect of early mouse embryonic development in vitro by co-culture with bovine and porcine oviductal epithelial cells (BOEC and POEC). The 2-cell embryos were collected from the oviducts of the superovulated and mated cultured in D-PBS /15% FCS at 48 hours after hCG injection. The in vitro developmental rate of blastocyst formation in the embryos were examined under the fllowing treatments; 1) TCM 199 added 15% HCS, 2) Ham's F-10 added 15% HCS, 3) MediCult IVF medium, 4) TCM 199 added 15% HCS + BOEC, 5) TCM 199 added 15% HCS + POEC, 6) Ham's F40 added 15% HCS + BOEC, 7) Ham's F-10 added 15% HCS + POEC,8) MediCult IVF medium + BOEC, 9) MediCult IVF medium + POEC. For a comparative study of in vitro development for 96 hours after hCG injection, were cultured with oviductal epithelial cell and media only. The obtained results were 2-cell embryos developed to the blastocyst stage in TCM 199, Ham's F-10 and MediCult IVF medium at the rates of 84.4,83.2 and 81.6%. respectively. The higher developmental rates(91~97%) of blastocyst formation was appeared when the embryos were co-cultured with a monolayer of bovine or porcine oviductal epithelial cells in TCM 199 or Ham's F-10 and MediCult IVF media. No significant difference in developmental rates was shown between bovine and porcine oviductal epithelial cells but significant difference in co-culture system in comparison between media only system and co-cultures. In conclusions, oviductal epithelial cells, BOEC and POEC, when co-culture with mouse early embryos improved the rates of development, blastocyst and hatching. Therefore, it is suggested that co-culture system using oviductal epithelial cells improve early embryonic developtnent in mouse.

This study was performed to establish the condition and the methods for the techniques of insertion the isolated blastomere cells into cytoplasm, in order to research the develop-mental ability of bovine embryo blastomere cells in vitro produced. After 24h in vitro ovary maturation with the ovaries from a slaughter house, in vitro fertilization was performed to the vital sperms which their mobility were decided by percoll gradient method, with 2~8 cell stage embryos, the blastomeres were isolated in +. +-free PBS, and following that embedded into agar and alginate solution, respectively. The rates of in vitro develop-ment are as follows ; in agar embedded 11 among 120(9.2%) 1 /2~1 /3 blastomers cleaved and 6 among 93(6.5%) 1 /4~1 /8 blastomeres cleaved. In sodium alginate-embedded 14 among 84(16.7%) 1 /2~1 /3 blastomeres cleaved and 6 among 85(7.1%) 1 /4~1 /8 blastomeres cleaved. In case of Na-alginate, the rate of the cells were better than those of agar. The results suggest that the techniques for embeeding the isolated blastomeres into gel may help cloning of bovine early embryo without nuclear transplantation.

This research was conducted to obtain the basic information on the cell block phenomenon occuring during early development in vitro of mouse embryos. Early embryos were recovered at 3h post-hGG injection(hph). Various chemicals (EDTA, EGTA, DTPA, MA and PRA) were tested to examine the effects of them on the overcoming the 2-cell block phenomenon. One hundreds M of the chelating agents were added to the M16 medium containing embryos. The treated embryos were worked and transferd to fresh M16 medium after 1, 3, 6 and 12h of treatment. Development was examined at 58 and l2Oph injection, respectively. 44.7~68.9% of the treated embryos developed to 4-cell stages at 58hph. Only 17.6~60.3% of the embyos developed upto blastocyst at l20hph. Whereas control embryos showed slightly lower development in M16 medium alone (38.9~42.4%, 4-cell and 3.8~65.5%, blastocyst). Three mitogenic agents were tested. 51.6~63.8% and 43.4~48.1% of embryos developed up to 2-cell and blastocyst stage, respectively when treated in 5 g PHA-M Imi for 5 min, 1, 3 and 6h subsequently cultured in fresh M16 medium. Control embryos only showed 38.8% for 4cell and 5.9% fo blastocyst at 58 and l2Ohph, respectively. 100M PMA was also beneficial for the 2-cell block. Showing better development them that of control (42.4 vs 57.9~59.4% 4cell and 5.9 vs 25.0~55.6% blastocyst, respectively. However 1M butyric acid was toxic to early embryos, thus arresting further development. These result indicate that either chelating or mitogenic agents could be used to overcome the "in vitro 2-cell block" occuring during early development in vitro of ICR embryosCR embryos

This experiment was arried out to investigate the development of ea4y rabbit embryos in vivo. Twenty-six New Zealand White does were superovulated by treatment with PMSG(Intervet Co; I. M single injection, 150. U./rabbit) followed 3 day later by simultaneous I.V injection of 100 I.U HCG (Intervet Co, )and natural service with fertile male. All of does was killed at the specific times (24, 27, 30, 36, 42, 50 and 93 h post-hCG) to find out the early embryonic development in vivo respectively. Embryos at the specified stages of development were obtained at the following times after injection of hCG; one-ceH at 24 h, two-cell at 24~27h, four-cell at 27~36 h, morulae at 50 h and early blasto-cyst at 93 h and expanded or hatching blastocyst at 144 h. Number of embryos recovered per rabbit superovulated was 26.1 and average of recovery rate was 83.7%. The results suggest that superovulation was efficient for the increase of embryo number in rabbits, and as shown in results, asynchronous cleavage was prevalent among the recovered embryos.

In vitro development of parthenogenetic embryo was examined after ethanol treatment of follicular oocytes matured in vitro for 42, 48, 54 and 60h in the pig. The follicular oocytes were matured in TCM 199 containing 15% FCS and gonadotrophins in an atmosphere of 39 5% . The cumulus-free oocytes were activated by 10% ethanol treatment in M2+4mg /ml BSA for 10 min. The ethanol-activated oocytes were washed and further cultured in TCM199+20%FCS containing granulosa cell monolayer. Maturation rates at 42, 48, 54 and 60h of IVM were 75.0, 86.5, 81.6 and 87.9%, respectively. Thus the oocytes maturated in vitro for longer periods did not improve nuclear maturation much. Pronuclear formation rates at 18h post-activation in ethanol-activated oocytes were 21.9, 25.0, 47.4 and 32.6%. The cytoplasmic maturation leading to pronuclear formation upon activation increased when the I VM period was extended from 42 to 54h. When the activated oocytes were cultured for 96~120h to analyse early development of the activated oocytes, the rates of embryonic development upto 5~8 cell were 5.3, 5.8, 12.0 and 11.7% among the cultured embryos. The result indicate that earlier development of activated porcine occyte is dependent on the duration of oocyte maturation, and that better development could be obtained from the oocyte matured for 54h.

In vitro developmental ability of early preimplantation monse embryos was shown to be depend on the embryonic stages, media and snpplements and their interaction(Experiment 1). The development of I-cell embryos were more promoted in the complex medinm(Ham's Fl0) than in the simple one(m-KRB), but that of 2-cell embryos showed the reverse effect. The bovine serum albumin(BSA) as a medium snpplement more promoted the development of I- and 2-cell embryos, compared with human fetal cord serum(HCS). On the other hand, the harmful effect of HCS was especially shown on the early cleavage in the embryonic development of the two stages. The effect of serum, in the respect of interaction between media and snpplements. was also more significantly appeared in m-KRB than Ham's Fl0. In the experiment 2, when the harmful effect of HCS was compared with that of fetal bovine serum(FBS), the former more promoted the development of l - and 2-cell embryos than the latter. The effect of HCS was more significantly shown in the development of I-cell than that of 2-cell embryos. Conclusively, as I- and 2-cell embryos were different in the requirements for the in vitro development. the optimal medium and supplement have to be selected for each embryonic stage. It is also respected to the better result if it take into consideration into the kinds of sera when serum is used for culture of early preimplantation embryos.

본(本) 연구(硏究)는 임간초지개량(林間草地改良)과 관리(管理) 및 이용(利用)의 기초자료(基礎資料)를 얻고자 차광정도(遮光程度)를 0%구(區)(自然光狀態), 25%, 50%, 75%차광구(遮光區)를 설치(設置)하고 Orchardgrass, Timothy, Perennial ryegrass를 '84년(年) 8월(月) 25일(日)에 파종(播種)하여 목초(牧草)의 동사(凍死) 및 월동전(越冬前) 초기생육특성(初期生育特性)을 조사(調査)하였던바 그 결과(結果

항로표지는 선박에 대하여 등광, 형상, 색채, 음향, 전파 등을 수단으로 운항 선박에게 위치, 방향 및 장애물의 위치 등을 알려주는 항행보조시설 일체를 의미하며, 주야간 선박항행의 안전을 위한 기본 수단이다. 예인선은 안전을 위해 종류별 최소 설비요건을 마련하여 사고를 대비하고 있지만, 예인삭의 길이, 조류의 영향, 상대적으로 중량이 큰 부선의 타력으로 인한 영향 등으로 예인선과 선회권이 달라, 변침 시 부선이 예인선을 따라가지 못하여 등부표와 추돌하는 사고가 발생하기도 한다. 본 연구의 목적은 두 선박의 물리적 관계를 분석하여 예인선 열을 식별하고 등부표와의 위험을 항로표지 관리운영 시스템을 통해 전파하는 기초 시스템을 제시하는데 있다. 연구를 통해 예부선의 사전 식별 가능성을 확인하였고, 위험 전파를 위한 메시지를 설계하고 모의 시스템을 통한 위험 전파 및 전자해도 상에 위험을 표시하였다.

품질의 변동성이 많은 순환유동층 플라이 애시를 현장에서 혼화재로 사용하는 것 보다 수경성 시멘트의 초기강도 발현을 위한 자극제로 활용할 수 있는 가능성을 판단하고자 하였으며, 이를 위하여 다양한 조건의 시멘트 조합을 사용하여 강도특성을 실험적으로 분석하였다. 실험을 통하여 순환유동층 보일러 플라이애시의 free CaO와 반응성 CaO가 수경성 시멘트 품질에 미치는 영향을 분석하였으며, 석고를 대체할 수 있는 결합재로 활용이 가능함을 확인하였다.

본 연구는 교사 간 협력 통한 내러티브 중심 교육과정 실행에서 교사의 경험 및 유아의 언어능력에 미치는 영향을 분석하였다. 연구 목적을 위해 혼합연구방법론을 적용하는 과정에서, 내러티브 중심 유아교육과정 실행 경험에 대한 교사 면담분석과 유아의 언어 능력 변화를 동시에 살펴보았다. 연구결과는 다음과 같다. 첫째, 교사의 협력은 새로운 나를 발견하는 시간, 함께 의미를 구성하는 시간, 아이들의 이야기에 귀 기울일 수 있는 시간으로 그 의미가 발견되었다, 둘째, 교사 협력의 경험은 유아의 언어발달에 유의미한 영향을 미친 것으로 나타났다. 따라서 교사 협력을 통한 내러티브 중심 교육과정의 실행은 교사의 교육과정 이론 구성과 유아의 언어 발달에 있어 유의미한 과정임을 확인할 수 있었다.

시멘트 산업은 탄소배출 감축을 위한 주요 산업분야로 고려되고 있으며, 콘크리트 분야에서 시멘트 사용량 저감을 위한 노력이 진행되고 있다. 이에 대한 노력의 일환으로 시멘트의 일부를 대체하여 사용할 수 있는 산업부산물을 다량으로 활용하기 위한 연구가 진행되고 있다. 산업부산물을 사용한 콘크리트는 내구성, 친환경성 및 경제성 등의 장점을 가지고 있으나, 응결시간 지연, 초기강도 저하 등의 문제점이 있다. 따라서 이 연구에서는 시멘트의 사용량을 줄이고 산업부산물인 플라이애시를 치환하여 사용하면서도 초기강도가 저하되는 문제점을 해결하는 것을 목표로 하였다. 이에 따라, 먼저 고분말도 시멘트 사용에 의한 수화반응 촉진으로 양생온도에 관계없이 모든 재령에서 강도가 증진되는 것을 확인하였다. 이어서 고분말도 시멘트에 플라이애시를 치환하여 강도발현 특성을 검토한 결과, 초기 재령에서도 보통 포틀랜드 시멘트와 비교하여 동등 이상의 강도 발현이 가능하였다. 또한, 플라이애시를 30%까지 치환하여도 수직․수평부재 거푸집 해체 시기를 단축할 수 있는 것을 확인하였다.

A new mandarin hybrid cultivar ‘Shinyegam’ was developed from a cross between ‘Kiyomi’ (Citrus unshiu × C. sinensis) and ‘Wilking’ (Citrus reticulata) at the Citrus Research Institute of the National Institute of Horticultural and Herbal Science in 2012. The ‘Jegam na No. 33’, a first selection seedling from the fruits obtained in 2008 was finally named ‘Shinyegam’ after field evaluation trials at five locations in Jeju island, Korea from 2010 to 2012. The tree vigor was intermediate and the tree was spreading in nature, similar to that of the ‘Kiyomi’ cultivar. The fruits produced were compressed to oblate and globose in shape, were a deep orange in color, and moderately weighed at 130 g. ‘Shinyegam’ produces fruit that mature in late December, and has few seeds, which are mono-embryonic. The average seed number of a fruit varied depending on the pollinator citrus tree. Cultivation of ‘Shinyegam’ along with the other cultivars possessing high pollen fertility resulted in seed numbers that was more than 20, while in the absence of a pollinator species the seed number was around 5. The fruit contained soluble solids at 11 to 12 ° Bx and the acidity was approximately 1% at maturity. The rind thickness of approximately 2.5 mm ensures easy peeling. The flesh is very tender and juicy, with a pleasant, aromatic flavor similar to that of ‘Wilking’ but is comparatively weak. ‘Shinyegam’ is moderately resistant to the citrus scab disease and melanose, but susceptible to citrus canker. Owing to the high quality of fruit, good aroma, and cold hardness characteristics, this cultivar is expected to satisfy consumer acceptability in terms of the diversity of the cultivar in open-field cultivation.

Early maturing glutinous rice lines with giant embryo were developed using anther culture. Deuraechan, mid-late maturing high-yielding japonica rice variety with resistance against rice stipe virus (RSV), bacterial blight (BB), and lodging, and Chenghyangna ge, early maturing glutinous rice germplasm with giant embryo were used the parents. F2 seeds from the cross between Deuraechan and Chenghyangna ge with glutinous endosperm and giant embryo were selected and propagated to F2 population. In F2 population, anther culture was conducted using the panicles from the early maturing plants. All doubled haploid (DH) lines showed early maturing, glutinous endosperm, and giant embryo phenotype. Through marker-assisted selections to Stvb-i and Xa3, 17 DH lines carrying both resistance genes were selected. Among 17 DH lines, six lines with more embryo size and better agronomic traits were selected and analyzed their characteristics. These lines were early maturing glutinous rice with giant embryo and showed enhanced yield, resistance against RSV and BB, and lodging, compared to previously developed giant embryo rice varieties. But they were vulnerable to preharvest sprouting which is important trait in early maturing rice. According to the texture and rapid viscosity analysis, DH lines were considered to have appropriate properties of cooked brown rice. They showed less hardness, gummniess, chewiness, and setback. Developed DH lines could be useful materials for diversification of cropping system and enhancing the brown rice consumption but the breeding efforts to improve the vulnerability against preharvest sprouting is required to apply for practical variety.

본 연구는 법무부에서 실시하고 있는 조기적응 프로그램의 추진 배경 및 운영 실태와 문 제점을 파악하여 조기적응 프로그램의 발전 방안에 대해 논의하고자 하였다. 이를 위해 중도입국 청소년을 대상으로 조기적응 프로그램 참가시간 및 교육 내용 등에 대한 만족도에 관한 설문조사에서는 총 51명의 학생이 참여하였다. 설문지 내용을 토대로 한 심층면접은 학생 8명, 현장 전문가 5명으로 실시하였다. 연구 분석결과 조기적응 프로그 램의 대상이 초․중․고등학생으로 함께 참여하고 있는데 아이들의 이해 수준과 집중도 차 이의 어려움, 법과 제도 및 질서에 대한 교재 내용 이해의 어려움, 한국 사회 정보에 대한 이론적인 이해의 어려움, 교육 내용을 이수하기에 너무 많은 내용으로 구성되어 있어 시간 의 부족함 등이 문제점으로 나타났다. 이러한 문제들을 해결하기 위한 발전 방안으로는 초․중․고등 분리교육 진행과 만화나 그림으로 이해하기 쉬운 교재개발의 필요성, 현장에 나가서 진행하는 현장체험학습과 같은 프로그램 추가, 한국인 및 본국 선배들의 멘토링 프 로그램 실시, 교육부 차원에서 지원해주는 조기적응 프로그램 교육을 받을 수 있는 연계과 정의 필요성 등 중도입국 청소년들이 한국 사회에서 범죄를 예방하고 사회생활과 학교생 활, 직장 생활을 잘 적응 할 수 있도록 제도적인 적응 지원방안이 마련되어야 함을 제안하 고 있다.