This study was in line with consecutive growth of urban agriculture implemented to provide a functional vegetable garden model for urban vegetable gardeners. It targeted the users who actually raise functional vegetable gardens, analyzed the flaws for correction in many ways including a type of products, quantity, ratio, and the like and existing problems. It is found out from Utilization that first is the highest ranking with no experience of growing vegetable garden education while growth education helping vegetable garden activity takes the half. Saying yes to the participation in the growth education following the process ranked the highest. In addition when planting, in regards to earning information about plantation timing and measure, they responded that they directly determine those by themselves. Second, the type of plant that they are mainly supposed to produce in family gardening was vegetables. Many say that it is easy to produce, easy to get information. In the fall, Kimchi and vegetables ranked the highest, the reason for functional vegetable gardeners to participate in planting family garden is an interest in a variety of producing crops. Third, the current amount included is proper for flower･herb plants in functional vegetable garden model while the ratio of crops vegetables60.5 : medical21.2 : flower･herbs18.3 in vegetable garden model is responded moderate.

Sensory evaluation, in vitro antioxidant activities and main compounds of coffee water-extract, coffee liqueur (CL) and coffee-ground liqueur (CGL) were investigated to consider their industrialization. Sensory evaluation showed that all groups of CGL without 25% CGL (3 month) were relatively higher than CL groups. Total phenolic compounds and in vitro antioxidant activities such as 1,1-diphenyl-2picryl-hydrazyl (DPPH) radical scavenging activity and ferric reducing/antioxidant power (FRAP) were also performed. The group of 35% CGL had higher total phenolic compounds than others, and the result of DPPH radical scavenging activity was similar to that of total phenolic compounds. In addition, 35% CGL is comparable to the FRAP of coffee water extract (CE). Qualitative and quantitative analysis using high-performance liquid chromatography (HPLC) were performed, and chlorogenic acid as a ployphenolic compound and caffeine as a nonpolyphenolic compound were detected in all samples. Moreover, the HPLC analysis showed that CGLs contain a larger amounts of chlorogenic acid (difference of 0.3~10.5%) and also greater amounts of caffeine (difference of 10.0~18.2%) more then CE. Consequently, these results suggest that coffee-ground as coffee by-products could be used as commercially available food substances because of its physiological molecules remained.

This study, examines the flow characteristics of upstream channel depending on water gate operation of Nakdan Multi-fuctional weir. The specific purpose of this study are to simulate the variation of flow velocity depending on the operation of the weir using 1-dimensional hydraulic model, HEC-RAS, and compare it with observed velocity. For discharge conditions from 50 ㎥/s to 3,500 ㎥/s, it is observed that the velocity of upstream channel is almost constant, whereas for probability flood discharge, the velocity and froude number are increased as the discharge values are increased. The velocity values for downstream boundary condition EL, 40.0 m are more decreased than those for EL. 40.5m. From comparison on the variation of water stage depending on water gate operation, it is observed that the stage values are almost constant for discharges below 300 ㎥/s, whereas 5 cm to 20 cm for discharges over 700 ㎥/s. Flow velocity at streamflow gauging station. Nakdong, is decreased by more than 875% after installing the weir. The results obtained from this study indicate that the velocity of upstream channel is decreased and the discharge and velocity of downstream channel are significantly varied after installing the weir.

The purpose of this study was to perform a functional components analysis and investigate the physical properties of powders made from the stems or fruit of freeze-dried Cheonnyuncho cactus (Opuntia humifusa). The functional components analysis showed that the stem and fruit powders han vitamin C levels of 42.14 mg and 105.21 mg, respectively. The stems powder contained more lutein than the fruit powder. The fruit powder contained more vitamin C than the stem powder. The SDF (soluble dietary fiber) and IDF (insoluble dietary fiber) in the stem powder were 45.24% and 22.15%, respectively, which were higher then the values for the fruit powder. The stem and fruit powders contained 19.30 mg/g and 25.10 mg/g of crude saponin, respectively. The pH of the stem and fruit powders was 5.34 and 5.07, respectively, both indicating low acidity. The L, a and b values of the stem powder color were 78.28, –3.71, and 19.19, respectively. The L, a and b values of the fruit powder color were 55.56, 24.84, and –3.18, respectively. The stems powder had a higher bulk density, water holding capacity, and swelling power than those of the fruit powder, but water-retaining capacity of the stem powder was lower than that of the fruit powder. In addition, the stems powder had a higher viscous material content and water uptake compared to the fruit powder. Based on the above results, we determined that Cheonnyuncho (Opuntia humifusa) powder had potentially useful functional components and physical properties.

Buckwheat sprouts are a vegetable; a functional food should provide health benefit and enhance performance as high nutritionally important substances. Buckwheat noodles are the major buckwheat food in Japan, Korea and China. In addition, Buckwheat as preventive medicine has undergone a great advancement in the last decade. Comparison of the functional properties distribution and utilization in tatary buckwheat is required of understanding the metabolites. The study was conducted to identify the sorts of phenolic compounds and metabolites in tatary buckwheat seedling at 4, 7, and 10 days seedling under the different combinations of lightemitting diode (LED) such as blue, red, mix (red, blue, and white), dark, and natural lights in stem and leaves. After breaking the dormancy, buckwheat seeds were grown in culture room under lights for 14 hrs and the dark condition for 10 hrs, at 25ºC for 10 days. Length of buckwheat was gradually increased under all of the conditions. Using HPLC, rutin was highest at 7 days under mix and natural light in stem and leaf, respectively. Quercetin was highest at 4 and 7 days under natural light in both. Chlorogenic acid was highest at 7 days under mix and natural in stem and leaf, respectively. Taken Together, this study indicates that phenolic compounds and metabolites present in those plants could be helpful for the human health and nutritional additive.

Crops are exposed to various environmental stresses. These have been affecting the growth of crops, resulting in the severe loss of agronomic production in many countries. Therefore, development of new varieties of resistant crops is required to assure the desired productivity of crops in stress conditions. In this study, a putatively stress-related gene BrTSR53 was isolated from Brassica rapa. The BrTSR53 is 481 bp long and contains ORF region of 234 bp. The expression of BrTSR53 was determined by quantitative real-time PCR analysis. After 3 hr, the highest quantities of mRNA were revealed in cold and salt stress treatments. In drought stress treatments, there was the highest expression after 36 hr. Therefore, it was confirmed that the ORF in BrTSR53 should be a gene that confer increased resistance to B. rapa growing in different stress conditions. The ORF region of BrTSR53 gene was cloned into an expression vector, pYES-DEST52, and a new protein with molecular weight of 13 kDa was detected by western blot analysis. Also, stress tolerance tests showed that BrTSR53-ORF transgenic yeast exhibited increased resistance to the salt stresses compared with the control. In conclusion, the present data predicts that novel ORF in BrTSR53 can serve as an important genetic resource for abiotic stress resistance.

UDP-glucose 4-epimerase (UGE; EC 5.1.3.2) is an enzyme that plays an essential role in the interconverts UDP-D-glucose (UDP-Glc) and UDP-Dgalactose (UDP-Gal). Five members of the Chinese cabbage (Brassica rapa) UDP-glucose 4-epimerase gene family, designated BrUGE1 to BrUGE5, have been cloned and characterized. Quantitative PCR shows that the BrUGE1and BrUGE4 mRNA are most abundant among other BrUGE genes, accounting for more than 55% of total BrUGE transcripts in most of the tissues examined. All genes showed organ specific expression pattern, two of which (BrUGE1 and 4) actively responded after Pectobacterium carotovorum subsp. carotovorum infection, while four genes (BrUGE-1, -3, -4 and -5)were shown to respond considerably against salt, drought and abscisic acid (ABA) treatments. To better understand the function of the UGE gene, we constructed a recombinant pART vector carrying the BrUGE1 gene under the control of the CaMV 35S promoter and nos terminator and transformed using Agrobacterium tumefaciens. We then investigated BrUGE1 overexpressing rice lines at the physiological and molecular levels under biotic and abiotic stress conditions. Bioassay of T3 progeny lines of the transgenic plants in Yoshida solution containing 120 mM Nacl for 2 weeks, confirmed that the BrUGE1 enhances salt tolerance to transgenic rice plants. Also T3 progeny lines of the transgenic plants, when exposed to infection caused by Xanthomonas oryzae pv oryzae, showed tolerance to bacterial blight. These results showed that BrUGE1 can be used as potential genetic resource for engineering Brassica with multiple stress resistance.

Rice gene functional annotation is greatly hindered due to functional redundancy. Based on OGRO database information, function of only 1022 genes were characterized previously where estimated expressed genes is approximately 50000. TFs protein class consist of 80 families and function of only 211 were reported. To address this issue, we developed web resource using MySQL, PHP and related frame work. Database integrates expression pattern and diverse data in phylogenomic contest. Since TFs plays diverse role in plants, meta-expression analysis would provide putative function of remaining genes. Using this approach and in-house database, we have identified featured expression groups: 228 belongs to anatomy, 224 to abiotic stress, 202 to biotic stress and hormone responsive group includes 267 genes. Out of 315 known genes through loss of functional studies, 294 genes have no closely related family members. Among 12 pairs with probes in database, 6 genes have PCC value with more than 0.5 among closely related genes. These data suggest that TFs showing more than 0.5 PCC value among closely relating family members more likely have functional dominancy. This study will provide useful functional information for whole rice TFs and suggest promising functional genomic studies.

Rice, as a model system of monocotyledon plants for genomic studies, is a main staple food for over half of the world population. A rice retrotransposon, Tos17, is active during tissue culture and its ability was wildly used in insertional mutagenesis. In this study we have produced 2,000 non-GM mutants induced by Tos17 in rice. We analyzed >2,000 flanking sequences of newly transposed Tos17 copies by the adaptor-ligation PCR method. The frequencies of Tos17 insertions in the genic and intergenic regions were 60.3% and 36.6%, respectively. We also selected four Tos17 insertion mutant lines for three TF genes which can be considered to be considered to be involved in rice seed development based on expression microarray data: osrem3, osta1, osbhlh1-1, and osbhlh1-2 mutant lines. According to Quadruple 9-mer-based protein binding microarray (Q9-UPBM) experiment, we found that the OsREM3, OsTA1, and OsbHLH1 bound to the ACACCAC, CACGTG, and GTAACA motifs, respectively. In combination of Q9-UPBM, RiceArrayNet analysis, and expression microarray data, we identified 8, 20, and 9 putative target genes of OsREM3, OsTA1, and OsbHLH1, respectively. We have been screening and characterizing the mutations by extensive phenotypic analysis as well as the functional analysis of genes.

국내 4대강에 설치된 다기능보는 하천을 횡단하는 수공구조물로써 고정보와 가동보로 구분되어 설치되었으며, 각 다기능 보의 수문운영 방식에 따라 유량이 변화하여 하상변동 및 유사이동 형태의 변화 가능성이 있다. 본 연구에서는 다기능보의 수문운영에 따른 장기 하상변동을 금강유역을 중심으로 연구하였다. 연구결과, 금강에서는 다기능보의 설치 및 수문운영 시나리오에 따라서 최심하상고의 변화가 연평균하상고의 변화에 비해서 상대적으로 큰 것으로 나타났다. 최심하상고의 하상저하는 최대 2.79 m, 하상상승은 최대 1.90 m까지 발생하는 것으로 나타났으며, 연평균하상고의 하상저하는 최대 2.16 m, 하상상승은 최대 1.24 m까지 발생하는 것으로 나타났다. 또한 하상변동에 따른 홍수위 분석 결과, 다기능보 설치 후에 홍수위가 대부분 상승하는 것으로 나타났으며, 최대 2.23 m까지 상승하는 것으로 나타났다. 이러한 결과로 인해서 제방의 여유고를 상회하는 홍수위가 발생할 가능성이 있기 때문에, 하천의 유사관리 및 하천계획수립을 함에 있어서 다기능보의 수문운영을 고려해야 한다고 판단된다. 또한 본 연구결과는 향후 하천계획을 수립함에 있어서 종단적 하도관리 및 안정하상 채택 등을 위해서 기초자료로 활용될 수 있을 것으로 본다.

본 논문에서는 경북 구미시 봉곡천에 최근 건설된 다기능어도를 대상으로 SAP2000으로 구조 해석하기 위한 변수를 R/C Slab, R/C+S/C Slab 및 지하이동통로 규격(가로×세로)을 1m×0.2m, 1m×0.4m, 1m×0.6m와 유속 0.8m/s, 1.2m/s, 1.6m/s으로 구분하여 해석한결과와 봉곡천 설계식을 비교하여 안정성을 검토하였다.봉곡천의 설계식 보다 R/C+S/C Slab 타입이 지하이동통로 출구부는 휨모멘트와 최대응력은 각각 28~54%, 26~50%, 측벽은 24~47%,17~31%, 상부슬래브인 경우도 10~27%, 4~20% 적게 나타났다. 따라서 최대응력과 휨모멘트가 R/C+S/C Slab 타입이 구조 안정성이 확보되는 것으로 나타났기 때문에 지하통로는 휨모멘트와 최대 응력이 27%, 25%, 측벽은 24%, 15% 상부슬래브는 14%, 10%의 보완이 요구되는 것으로 판단된다.이러한 결과는 지하이동통로 규격이 봉곡천 규격과 동일한 1m×0.4m일 때가 1m×0.2m, 1m×0.6m 보다 안정성이 가장 유리한 것으로확인되었다. 또한 해석 및 분석 결과를 근거로 다기능어도 시공 시 기본 자료로 활용이 기대된다.

In this study, the multi-functional fishway with underground passage, sediment block system reflect the fish's ecological characteristics and many kinds of fish up and down easily is proposed. The 3D-analysis of Reinforced Concrete(R/C) Slab and R/C + Steel Concrete(S/C) Slab with rectangular underground passage are carried out by SAP 2000 software program for multi-functional fishway's durability. The Slab analysis results show that the R/C Slab type of the maximum stress is decreased by 33.5 than that of R/C + S/C type(Size:1m x 0.4m, Velocity: 1.6m/sec)and the shortage of shear is 2 than that of R/C + S/C type(Size:1m x 0.4m, Velocity: 1.6m/sec). The stability of R/C + S/C concrete slab with underground passage is more advantageous than that of R/C concrete slab.

2-acetyl-1-pyrroline (2AP) was widely known as the principal aroma compound, it development in rice has been reported due to the loss of function of betaine aldehyde dehydrogenase gene (badh2) on chromosome 8. In previous study, a lot of haplotypes have been found of this gene, while only limited haplotypes have been proved as functional mutations. A total of 137 core set accessions, and additional 45 germplasms have been employed in this study. Finally, two new mutations have been found (3bp deletion in exon12 and C/A SNP in exon 10), and 23 haplotypes have been detected, most of them had strong relations with aroma formation. According to the sequence results, five functional markers have been developed, the markers showed a highly efficient in discriminating the special aromatic rice varieties, and displayed perfect co-segregation with the trait of fragrance in F2 population. Those new markers developed in the present study would be useful in molecular breeding of fragrant rice varieties. Based on the haplotypes, the further research is in progress.

Glutamine synthetase (GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. Exposure of plants to cadmium (Cd) has been reported to decrease GS activity in maize, pea, bean, and rice. To better understand the function of the GS gene under Cd stress in rice, we constructed a recombinant pART vector carrying the GS gene under the control of the CaMV 35S promoter and OCS terminator and transformed using Agrobacterium tumefaciens. We then investigated GS overexpressing rice lines at the physiological and molecular levels under Cd toxicity. The GS activity along with mRNA expression were found higher in transgenic than in wild type plants. And this is validated by the low malondialdehyde contents observed 10 days after treatment. GS overexpression in rice resulted in the modulation of expression of enzymes responsible for membrane peroxidation, which may result in the sudden death of plants. Our results thus describe the features of a transgenic rice plants with enhanced tolerance to Cd toxicity.