5-axis laser cutting has great advantages when it is applied to three dimensional machining requiring high cutting quality. For developing 5-axis CNC laser cutting systems, however, many problems such as rotating a laser head or a working table, 5-axis se

Body and head lice (Pediculus humanus humanus and Pediculus humanus capitis, respectively) are typical ectoparasites of humans. They differ not only in the ecological habitat but also in the vector competence in spite of their conspecific nature. Only body lice transmit several bacterial pathogens to humans, including Bartonella quintana, Rickettsia prowazekii and Borrelia recurrentis. In this study, the proliferation rates of two model bacteria, a gram positive Staphylococcus aureus and a gram negative Escherichia coli, were determined following bacterial challenge by cuticular injection. Both bacteria proliferated rapidly in body lice at the early stage of bacterial challenge but not in head lice, suggesting that head lice have more sensitive immune responses to these bacteria. In vivo phagocytosis assay revealed that head lice have much higher phagocytic activity against E. coli than body lice whereas only slight differences in phagocytic activity against S. aureus were observed between the two lice species. Taken together, these findings suggest that the reduced phagocytosis activity of body lice contributes, at least in part, to their higher vector competence.

The differences in the immune response between body lice, Pediculus humanus humanus, and head lice, Pediculus humanus capitis, were regarded as primary factors determining their differential vector competence. To find any differences in genetic components in immune system between body and head lice, whole genome sequences of head lice were determined by both SBS [sequencing by synthesis, Illumina Genome Analyzer (Illumina-GA)] and pyrosequencing (Roche GS FLX), and compared with the reference genome sequences of body lice. The short DNA reads from Illumina-GA (an average mapping depth of 50-fold) were aligned first to the body louse reference genome, to which Roche GS FLX DNA reads (an average depth of 2.5-fold) were subsequently assembled to make up gaps between mapped consensus. Total consensus showed a size of 114 Mb and a coverage of 96% of the published body louse genome sequences. From this head louse genome sequences, a total of 12,651 genes were predicted and used for comparing with the 10,775 genes previously reported from the body louse genome. The homolog analysis identified 873 head louse-specific genes and 422 body lice-specific genes. Comparison of immune response genes between both louse species showed head lice have more number of immune-related genes than body lice. Head lice were determined to possess all of the 107 immune-related genes reported in the previous study (Kim et al., 2011), suggesting that there is no difference in genetic make-up in terms of the 107 immune-related genes between body and head lice.

Angelica decursiva has been used in Korean traditional medicine as an antitussive, an analgesic, an antipyretic and a cough remedy. However, the anti-cancer properties of Angelica decursiva have not yet been well defined. In our current study the cytotoxic activity of ethanol extracts of Angelica decursiva root (EEAD) and the mechanism of cell death exhibited by EEAD were examined in FaDu human head and neck squamous cell carcinoma cells. The cytotoxic effects of EEAD upon the growth of FaDu cells were examined with an MTT assay. In addition, the mechanism of cell death induced by EEAD was evaluated by DNA fragmentation analysis, immunoblotting and caspase activation measurements. EEAD induced apoptotic cell death in FaDu cells in a concentration- and time-dependent manner, as determined by MTT assay and DNA fragmentation analysis. Furthermore, the proteolytic processing of caspase-3, -7 and -9 was increased by EEAD treatment of FaDu cells. In addition, the activation of caspase-3 and -7 was detected in living FaDu cells by fluorescence microscopy. These results suggest that EEAD can induce apoptosis and suppress cell growth in cancer cells and may have utility as a future anticancer therapy.

This study compares the collocation of Korean and Mongolian language, using the noun 'head', which indicates the part of body. The purpose of this study is to analyze the semantic and syntactic aspects coping of collocation and give the linguistic explanation to the regularly appearing collocation aspects coping.The collocations can be defined as a nuclear of collocation which has transparent meaning, and limited combination of old vocabulary, which has no transparent meaning. Korean noun 'head' participates in the collocation usually with the meanings such as 'part of body, hair, inside of the head, spirit'. Especially, the meaning 'hair' appears the most frequently. In this case, the meaning of the 'head' equals to 'us', not the 'tolgoi'. This is because, mongolian noun 'tolgoi' has no meaning of 'hair'. Only one exception exists, where 'wash the head/wash the hair' can be represented in mongolian collocation “tolgoi ugaah/u's ugaah”. (wash the head/wash the hair) Consequently, among the 31 korean collocations there can be 27 mongolian collocation corresponding forms. And 16(+1) of them are composed with meaning 'tolgoi'. Furthermore, this study analyzes the mongolian word 'tolgoi' as a starting point and searches for its collocation corresponding forms. Mongolian noun 'tolgoi' has participated in the collocations with the meanings such as 'part of the body, spirit'. Among 22 collocations with the word 'tolgoi', there exists 16 korean collocation corresponding forms. 14 of them were with the meaning 'head'. In comparing the lexical semantic representation, mongolian and korean collocations may be equal. But the verbal meaning is not equal. And there are occasions where collocation corresponding forms does not exist at all. In this case, they can be interpreted by free join or an independent word. Syntactically, korean and mongolian collocations have shown almost the same configuration.

차선을 벗어난 승용차가 기초에 강결된 표지판 지주에 충돌할 경우 차량이 받는 충격과 변형은 탑승자의 안전을 위협하게 되므로 분리지주를 쓰는 것이 일반적이다. 국도에서 1200mm×1200mm의 단일노선표지에 사용되는 직경 101.6mm, 두께 4.0mm의 강관지주가 기초에 강결되었을 때와 Clip형 분리장치를 갖는 경우에 대한 820kg-50kg-정면 충돌 시 차량과 지주의 거동을 LS-DYNA 시뮬레이션을 통하여 비교 분석하였다. 분리식 지주 충돌 시뮬레이션 결과는 동일한 조건의 충돌실험 결과와 비교하여 신뢰도를 높였다. 강결된 원형지주에 대한 충돌 시뮬레이션 결과 탑승자 안전지수(THIV, PHD)와 차량의 변위가 탑승자의 안전을 위협하는 수준으로 나타났으나 Clip형 분리장치를 갖는 지주에 대한 충돌 시뮬레이션 결과 충돌 시 지주의 분리로 감속도가 현저히 줄어들어 안전한 것으로 확인되었다.

The purpose of this study was to measure intra-rater and inter-rater reliability and range of motion for measurement of passive shoulder internal rotation range of motion and to compare anterior glide distance of humeral head in three methods. Fifty healthy subjects and fifty patients with shoulder musculoskeletal pain were recruited for this study. The subjects' passive shoulder internal rotation range of motion was measured by visual estimation, manual stabilization, and pressure biofeedback unit methods. In two trials, measurements were performed on each subject by two examiners. Intraclass correlation coefficient (ICC(3,1)) was used to determine the reliability of each measurement. The intra-rater reliability of the three methods was excellent (ICC=.77~.93) in both groups. The inter-rater reliability of the visual estimation method was poor (ICC=.20, .29), the manual scapular stabilization method was poor and fair (ICC=.09, .50), and the pressure biofeedback unit method was excellent (ICC .86, .75) in the experimental and control groups. In the experimental group, the difference of examined range of motion by each examiner was significant in the visual estimation method and manual scapular stabilization method, but there was an insignificant difference between the groups is the pressure biofeedback unit method. This result suggests that the intra-rater and inter-rater reliability of a pressure biofeedback unit was better than the other methods. The difference in distance of the anterior glide of humeral head was insignificant among all the methods. The pressure biofeedback unit method was the most reliable method, so it is proposed to be a new and reliable method to measure internal rotation range of motion.

Domestic SUV were evaluated for rating the ability of seat and head restraints to prevent neck injury in low speed rear-end crashes. The dynamic test criteria are divided into two groups-seat design parameters(Initial evaluation) and test dummy response parameters(Dynamic evaluation). Head restraint designs with initial geometric ratings of “acceptable" or “good" will be tested in a simulated 16 km/h rear impact to assess whether they provide effective dynamic support for the torso, neck and head. In the measurement and rating of static head restraint geometry, the initial static geometric rating of the domestic SUV cars were “good" except the A-car. In the final rating of the seat and head restraints, W-Car was “good", the other cars were “poor". The performance of dynamic test for the most part of the domestic SUV cars were “poor" and it is necessary to improve.

The human embryonic-lethal abnormal vision-like protein, HuR, stabilizes mRNA containing adenine- and uridine- rich elements in their 3’untranslated region. Because cyclooxygenase-2 (COX-2) mRNA is a cellular transcript that contains an adenine- and uridine-rich element, it can be regulated by the HuR protein. In this study, we examined the relationship between COX-2, HuR, MVD, and the clinicopathological parameters. Nineteen out of 43 cases of HNSCC showed high level of COX-2expression, and 68% of these patients showed high COX-2 immuno-reactivity indicating the strong expression of the cytoplasmic HuR protein. Also, MVD expression in the cases with high COX-2 expression was higher than in the cases with low COX-2 expression. These results suggest a strong correlation between the overexpression of cytoplasmic HuR and COX-2 expression in HNSCC, and that COX-2 is associated with MVD in HNSCC. In conclusion, COX-2 regulated by cytoplasmic HuR may be a good tumor angiogenic factor in HNSCC.

목적 : 본 연구는 머리 각도에 따른 목뿔위근육의 활성 정도를 확인하여 임상에서 삼킴 기능 증진을 위한 치료적 적용시 목뿔위근육들이 보다 활성화 되는 자세를 확인하고 이를 치료에 적용하기 위하여 시행되었다. 연구 방법 : 대전광역시 OO대학교에 재학중인 학생들을 대상으로 실험참가 대상자들을 모집하여, 지원자 중 무작위로 39명의 학생(남 24명, 여 15명)들을 선정하여 실험을 진행하였다. 프랑크포트 수평면이 지면과 수평이 되는 각도를 기준으로 머리각도 중립 자세(head flexion 0°)를 설정하였으며, 방사선 촬영을 통하여 머리각도 15°하방, 머리각도 15°상방 상태를 확인한 후, 각각의 상태(머리 중립 0°, 머리 굴곡 15°, 머리 신전 15°)에서 3회씩(1회 삼킴 후 10초 휴식) 마른 삼킴과 젖은 삼킴을 시도하였다. 결과 : 마른 삼킴과 젖은 삼킴 모두에서 중립시보다 머리 각도를 15°굴곡하였을 경우 목뿔위근육들이 보다 활성화되는 것을 알 수 있었다. 이에 반하여 머리 각도를 15°신전하여 삼킴을 시도하였을 경우에는 통계적으로 유의할만한 결과를 보이지 않았다. 결론 : 머리를 굴곡시킨 자세에서 삼킴을 유도하는 것이 좀더 목뿔위근육을 활성화시키고 목뿔의 가동성을 높여, 보다 안전한 식이를 위한 방법으로 제안될 수 있을 것이다.

To search for hyper-variable genetic markers that can distinguish regional populations of head lice, we screened the inter simple sequence repeats (ISSR) based on the genome database of body louse, which is closely related conspecific species. An ISSR mining software, SciRoKo 3.4, was employed to excavate ISSR markers from the genome database under the MISA mode (≥ 60 bp repeats). Entire body louse genome (ca.100 Mb) was loaded to SciRoKo for ISSRs mining. A total of 5,336 ISSRs were obtained, and primers specific to individual ISSRs were designed by the Primer 3 and DesignPrimer 1.0 softwares. In order to prove the compatibility of body louse ISSRs to head lice, 31 PCR primers were randomly chosen out of a total of 613 pairs, and their appropriateness was tested by comparing the amplified PCR band patterns between body and head lice. Eleven primer pairs that resulted in poor or little amplification were excluded, and 20 primer pairs were further tested for three head louse populations (California, Panama and Chung-ju, Korea). Finally, nine primer pairs ensuring robust amplification of highly variable band patterns were selected to use for population genetic study of head lice.

This study was conducted to investigate specific stages and distribution of overwintering nymphs of green rice leafhopper, Nephotettix cincticeps by measuring head capsule width. The nymphal head capsule width of the 1st instar to the 5th instar was 0.381, 0.502, 0.673, 0.979 and 1.128 ㎜, respectively. Its coefficient variation was 5.3, 4.0, 3.0, 4.5 and 5.3%, respectively. Growth ratio of each instar was not significantly different among 2nd to 4th instars as 1.31 to 1.34, but for the 5th instar it decreased as 1.28. The logarithm of the nymphal head capsule width was regressed as a function of the stage number of insects, resulting in LogY = 1.4627 + 0.1192X (r² = 0.9993). Also fitness to the Dyar’s law for the nymphal head capsule width of each instar was 98% or over and the Dyar’s constant (K) was 1.316. The occurrence of N. cincticeps was maximum at the end of January with 195 individuals/0.25 ㎡. The most abundant instar in the overwintering N. cincticeps population was 4th instar accounting for over 90%. Population of the 5th instar began to increase from mid-March, and adults began to occur in early April.

Body lice (Pediculus humanus humanus), obligatory human ectopasites, differ from conspecific head lice (Pediculus humanus capitas) in the choice of habitat and the capacity of disease transmission. Only body lice are known to naturally transmit a variety of human diseases, including epidemic typhus, trench fever and relapsing fever. Such differences in vectoral capacity are expected to be due to their differences in immune responses during pathogen invasion. Here, we annotated 94 immune related genes from the body louse genome and determined the differences in the transcription profiling of immune related genes between the head and body lice by qrt-PCR. In general, head louse females showed more sensitive immune responses than body louse females to Staphylococcu. aureus dermal challenge as judged by selective induction of defensin 2 in head lice. In contrast, when the 3rd nymphs were orally challenged, body lice exhibited more sensitive immune responses than head louse to Escherichia coli as judged by selective induction of defensin 1 and PGRP in body lice. These stage- and pathogen-specific differences in immune responses should provide basic insight on the vector competencies in the head and body lice.

I look at the images of Maud Gonne in Yeats's "Bronze Head." The bronze head is a sculpture made by Lawrence Campbell, which is in the Municipal Gallery of Modern Art, Dublin. When Yeats saw it, he must be shocked; she is old, and yet she looks "magnificent." In the bronze work, Yeats sees Maud Gonne as "human, superhuman," and "supernatural," as well. He puts down all that occurs to him, from the very first encounter, when "she walks like a goddess," not without wildness, though, to the image of Cathleen-like soul, to the image of her being supernatural with a sterner eye. All this enriching vision is made possible in this last poem of his; it is a conclusion to his poetry that is a history of a great heart craving for life for anther great heart; it is the best paean dedicated to a Goddess in his heart.

Determining age distributions of insect is required for many basic ecological studies. Most of insect developmental studies, the head capsule width and other developmental traits have been widely used to distinguish the instars. Unlike to other insect species, collembolan continues to molt after they reach sexual maturity. So these traits sometimes make it difficult to conduct developmental studies. Model animal used in this study was Paranura rosea a native collembolan speciese. To determine the age groups of P. rosea, we periodically (1week) made digital images of P. rosea (over 100 different images) under the magnifier (DIMIS-M) for consecutive 20 week. And then the head capsule width and the surface area of P. rosea were analyzed using a image software (ImageJ). In this test the head capsule widths and surface area were set as developmental parameters. Both two developmental parameters showed time-dependent increase patterns. And they gave good agreements between predicted developmental parameters and observed parameters (r2=0.83 and 0.71). Based on the reproducibility of juveniles and statistical mean separation tests (tukey's test), both two developmental parameters could be divided into 3 age groups. The information given this study will be used as a basis for understanding the population dynamics and other ecological research.

In this study, alterations in whole proteome expression patterns in the head of Japanese medaka (Oryzias latipes) was investigated following acute or chronic exposure to benzo(a)pyrene (BaP) (25 μg L-1) for 48 hrs and 15 days, respectively. The

Amino acid transporters are essential for the growth and proliferation in all living cells. Among the amino acid transporters, the system L amino acid transporters are the major nutrient transport system responsible for the Na+-independent transport of neutral amino acids including several essential amino acids. The L-type amino acid transporter 1 (LAT1), an isoform of system L amino acid transporter, is highly expressed in cancer cells to support their continuous growth and proliferation. 2-Aminobicyclo-(2,2,1)-heptane-2-carboxylic acid(BCH) is a model compound for study of amino acid transporter as a system L selective inhibitor. We have examined the effect and mechanism of BCH on cell growth suppression in FaDu human head and neck squamous cell carcinoma. The BCH inhibited the L-leucine transport in a concentration-dependent manner with a IC 50 value of 43.8±4.3μM. The majority of L-leucine uptake is, therefore, mediated by LAT1 in FaDu cells. The growth of FaDu cells was inhibited by BCH in the time- and concentration- dependent manners. The formation of DNA ladder was not observed with BCH treatment in the cells. Furthermore, the proteolytic processing of caspase-3 and caspase-7 in the cells was not detected by BCH treatment. These results suggest that the BCH inhibits the growth of FaDu human head and neck squamous cell carcinoma through the intracellular depletion of neutral amino acids for cell growth without apoptotic processing

A test was conducted using two high density discharge lamps, the H2D and the structurally new H4D. They were tested for luminous illumination and luminous temperature in the day and night time. The test was conducted without crippling the performance of the H2D by adding a magnetic actuator, enabling it to move left to right, and up and down. By making these modifications we constructed a sample of the H4D. We compared the H2D and the H4D sample's luminous illumination and luminous temperature by using a photometer and a digital thermometer in the day and night time. We discovered that the H2D and H4D performed similarly from the data we gathered. Now we know the H4D has potential use and extensive research needs to be made to gather more detailed data.

본 연구에서는 아스팔트 혼합물의 변형강도시험에서 하중봉의 치수가 변형강도 특성에 미치는 영향을 평가하였다. 이를 위하여 직경(D) 40mm, 하단의 원형 절삭 반경(r)을 10.0mm와 10.5mm로 제작한 두 개의 하중봉으로 아스팔트 혼합물의 변형강도를 측정하고, 반복주행시험을 수행하여 얻어진 소성변형 특성치와 상관성 분석을 하였다. 또한 하중봉의 원형 절삭반경에 따른 골재치수에 어떠한 영향을 미치는지를 확인하기 위하여 통계 분석을 하였다. 변형강도 값과 변동계수는 r=10mm하중봉을 사용한 것이 더 낮은 것으로 나타났다. 또한 r=10mm 하중봉이 변형강도와 소성변형 특성치와 상관성 분석에서도 더 높은 상관성을 보였다. 골재치수와 원형 절삭반경은 변형강도에 영향을 미치는 것으로 나타났으나 두 변수의 교호작용에서는 골재의 크기가 10mm~19mm내에서는 r에 영향을 받지 않는 것으로 나타났다. 따라서 소성변형과의 상관성이 더 우수하며 변동계수도 낮은 것으로 나타난 D=40mm, r=10.0mm의 하중봉을 사용하는 것이 적합할 것으로 판단되었다.

A quantitative sequencing (QS) protocol that detects the frequencies of sodium channel mutations (M815I, T917I and L920F) responsible for knockdown resistance in permethrin-resistant head lice was tested as a population genotyping method. Genomic DNA fragments of the sodium channel α-subunit gene that encompass the three mutation sites were PCR-amplified from individual head lice with either resistant or susceptible genotypes, and combined together in various ratios to generate standard DNA template mixtures for QS. Following sequencing, the signal ratios between resistant and susceptible nucleotides were calculated and plotted against the corresponding resistance allele frequencies. Quadratic regression coefficients of the plots were close to 1, demonstrating that QS is highly reliable for the prediction of resistance allele frequencies. Prediction of resistance allele frequencies by QS in several globally collected lice samples including 12 Korean lice populations suggested that permethrin resistance varied substantially amongst different geographical regions. Three local populations of Korean lice were determined to have 9.8-36.7% resistance allele frequencies, indicating that an urgent resistance management is needed. QS should serve as a preliminary resistance monitoring tool for proper management strategies by allowing early resistance detection.