We analyzed the performance of hubless rim propellers based on the number of blades, maintaining a fixed pitch ratio and expanded area ratio, using computational fluid dynamics (CFD). Thrust coefficient, torque coefficient and efficiency according to the number of blades were analyzed. In addition, the pressure distribution on the discharge and suction sides of the blade was analyzed. As the advance ratio increases, the thrust coefficient decreases. The highest thrust was shown when the advance ratio was lowest. For the three, four, five and six-blades, the torque coefficient tended to decrease as the advance ratio increased. In the case of seven and eight-blades, the torque coefficient tended to increase as the advance ratio increased. The maximum efficiency was found when the advance ratio was 0.8. When the three-blade, it showed high efficiency at all advance ratios. A high pressure distribution was observed at the leading edge of the discharge blade, and a low pressure distribution was observed at the trailing edge. Applying a hubless rim-driven thruster with the three-blade can generate higher thrust and increase work efficiency.

The towing distance, which is speed over the ground, and the water flow quantity, which is speed through the water, were used when estimating the amount of Metapenaeus joyneri resources that rose to the surface at night using the swept area method in order to compare and analyze the difference. It was conducted using a shrimp dredge, trial fishing gear for catching Metapenaeus joyneri. Catch during the entire survey period was 188.9 kg. Monthly catch ranged from 3.1 to 109.2 kg, highest in June and lowest in September. The swept volume calculated using the speed over the ground was about 13% higher than using the speed through the water. Metapenaeus joyneri resources estimated using the towing distance ranged from 320.1 to 14,649.8 kg. Resources estimated using the water flow quantity ranged from 278.5 to 12,886.3 kg. Therefore, the amount of Metapenaeus joyneri resources estimated using the speed over the ground was about 14% higher than the method using the speed through the water, indicating that the amount of resources was overestimated.

Organic wastewater causes serious environmental pollution, and catalytic oxidation is promising technique for wastewater treatment. Developing green and effective catalysts is currently challenging. In this work, green synthesis of nano zerovalent iron loaded onto porous biochar derived from popcorn is conducted, and catalytic oxidation of Rhodamine B (RhB) is evaluated in the presence of H2O2. Effect of process factors is examined on catalytic performance for RhB removal. The mechanism of RhB removal is discussed by characterizations (Fourier transform infrared spectra and Raman) and UV–vis spectra. RhB removal is improved with high catalyst dosage, low initial RhB concentration, and high reaction temperature, while it is slightly influenced by carbonization temperature of biochar, H2O2 dosage and pH value. Under conditions of BC-250 1.0 g/L, H2O2 0.01 mol/L, pH 6.1, and temperature 30 °C, the removal rate of RhB is 92.27% at 50 min. Pseudo first-order kinetics is used to fitting experimental data, and the activation energy for RhB removal in BC-250/H2O2 system is 39 kJ/mol. RhB removal in BC-250/H2O2 system can be attributed to adsorption effect and catalytic oxidation with the dominant role of hydroxyl radical. This work gives insights into catalytic oxidation of organic wastewater using green catalyst.

The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

예이츠는 성실한 연구와 주변 철학자들과의 지적인 교류를 통하여 플라톤과 플로티누스에 대한 깊은 이해력을 키웠다. 특히 플로티누스의 철학 체계인 신플라톤주의가 그의 작품 전반에 걸쳐 널리 미치는 영향을 인식하는 것은 그의 작품을 이해하는데 필요한 귀중한 통찰력을 준다. 예이츠의 신비주의와 비교가 주목을 받아온 반면, 예이츠의 시에서 신플라톤주의는 상대적으로 충분히 탐구되지 않았다. 본 논문은 예이츠의 시, 특히, “The Tower”에 얽혀있는 이러한 주제들을 밝히고자 한다. 예이츠는 “The Tower”에서 풍부한 상징주의와 우화를 사용하여 이 시를 신플라톤주의를 탐구하기 위한 이상적인 장으로 만들었다. “The Tower”를 통하여, 영혼의 상승, 물질적 한계의 초월, 지상과 천상의 상호 작용이라는 신플라톤주의의 철학적 주제들이 탐구되어질 수 있다. 시 속의 탑은 신플라톤주의의 핵심인 더 높은 진리를 향한 영혼의 추구와 물리적 세계와 영적 세계 사이의 연결을 상징한다.

본 연구는 특화된 문화관광지이자 주제관광의 대상이기도 한 한방식물원 탐방객의 인식 분석을 통해 한방식물원의 설계와 관리 방향을 제시하고자 한 것이다. 중국 하북성 안국시의 약박원(藥博園)을 사 례로 지각된 가치가 탐방객의 정서 및 편익에 미치는 영향을 밝히고자 AHP-엔트로피 가중치를 통해 한방식물원 탐방객의 지각된 가치 지표의 가중치를 계산하는 한편 요인분석을 통해 요인과 지표의 관 계를 탐색하였다. 본 연구에서는 경관평가 연구에서 일반적으로 적용하는 지표 가중치 분석방법을 탐 방객의 지각된 가치 척도 도구로 활용하였다. 그 결과, 자극 내부요인반응(S-O-R)이론의 관계를 바탕으 로 ‘탐방객의 지각된 가치’, ‘정서’, ‘지각된 편익’ 간의 관계를 설명하는 구조 방정식 모델에서는 다른 요인과는 달리 ‘관리 서비스 지각가치’는 탐방객의 지각된 편익에 유의한 정(+)의 영향을 미치지 않는 것으로 나타났다. 또한 AHP 계층분석법 및 엔트로피(Entropy) 가중치법을 통해 탐방객의 지각된 가치 지표에 관한 주·객관적 복합 가중치를 계산한 결과, ‘환경 지각 가치’의 가중치가 가장 높은데 반해 ‘관 리서비스 지각 가치’의 가중치는 가장 낮은 것으로 밝혀졌는데 이는 구조방정식의 상관관계 검정 결과 와 일치하는 것이다. 궁극적으로 한방식물원의 주변의 ‘환경’은 탐방객의 체험 향상에 가장 중요한 가 치임이 확인되었다.

This research investigated the immunoenhancing effect through the intracellular MAPKs and NF-B signaling pathways in macrophages activated by crude polysaccharides (YBP) of barley sprouts. YBP extracted from barley sprouts is composed of xylose (25.8%), arabinose (24.1%), galactose (23.4%), and galacturonic acid (11.7%). YBP did not affect the cytotoxicity and showed superior secretion of nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor (TNF)- by RAW264.7 cells. Also, YBP dose dependently increased IL-6, TNF-, and inducible nitric oxide synthase (iNOS) mRNA gene expression. In the western blot, YBP strongly induced the phosphorylation of the p38, JNK, ERK, and IB pathways in RAW 264.7 cells. In the anti-pattern recognition receptor (anti-PRRs) assay, the effect of YBP on NO secretion strongly decreased toll-like receptor (TLR) 4 and Dectin1 antibodies, whereas IL-6 and TNF- secretion by YBP mainly decreased SR and CD14. Therefore, we concluded that YBPinduced NO, IL-6, and TNF- were secreted via the MAPKs, while NF-B pathways through TLR4, Dectin1, SR, and CD14 receptors existed in a macrophage surface and were involved in the immunoenhancing effect.

자생식물은 관상용, 약용, 식량자원으로 활용될 수 있는 잠재력을 지닌 고유 유전자원이다. 돌부추(Allium koreanum H.J. Choi & B.U. Oh)는 우리나라 해안 암반지대에 분포하는 자생식물 중 하나로, 기후변화와 서식지 감 소로 인해 보전 가치가 높은 식물이다. 이번 연구는 온도와 과산화수소가 돌부추의 발아에 미치는 영향을 조사하기 위해 수행되었다. 종자를 무처리(대조군) 또는 1% 과산화수소(H2O2)로 90분간 처리한 종자를 준비해 15, 20, 25°C 로 설정된 식물 생장 챔버에 배치하였다. 그 결과, 파종 23일 후 15°C에서 42%인 발아율이 20°C와 25°C에서 각각 18%와 0%인 발아율보다 2배 이상 높았으며, H2O2 처리 여부와 관계없이 15°C에서 발아율이 42%로 가장 높았다. H2O2 처리와 관계없이 최종 발아율 50%(T50)에 도달하는 일수는 20°C에서 가장 짧았지만, 일평균 발아율(MDG)은 15°C에서 가장 높았다. 따라서 1%의 H2O2 처리는 돌부추의 발아율에 큰 영향을 미치지 않았으며, 15°C의 온도가 돌부추의 발아율을 높이는 데 최적인 것으로 판단된다. 본 연구 결과는 돌부추의 발아를 위한 기초 연구 자료로 활 용될 수 있을 것으로 기대된다.

In the genus Asobara Foester, 1863 (Braconidae: Alysiinae), two new species, Asobara rotundata sp. nov. is described and illustrated. In addition, the DNA barcode region of the mitochondrial cytochrome c oxidase subunit I (COI) were sequenced for the species. An identification key for the Asobara species officially recorded from Korea is provided.

Bacillus thuringiensis (Bt) is currently the most commonly used microbial pesticide. In the previous study, Bt IMBL-B9 known for its high toxicity against Spodoptera exigua, S. frugiperda and Plutella xylostella was characterized. To develop novel biopesticide, optimization of culture medium is required for the cost-effective mass production for toxin production of IMBL-B9. Through experimental design by Plackett-Burman design, ingredients that significantly influenced the production of IMBL-B9's toxin were selected. Using these results, the novel culture medium for IMBL-B9 was developed and the toxin yield of IMBL-B9 was significantly increased than conventional media by using this medium. These results could be useful for the development of biopesticides.

Stilbene-based fluorescent brighteners (FB) have been shown to enhance insecticidal activities of entomopathogenic viruses but little is known its effect on entomopathogenic bacteria. We investigated the effect of two FBs (FB 28 and FB 71) on the insecticidal activity of B. thuringiensis var. kurstaki (Btk) as well as the Lymantria dispar multiple nuclear polyhedrosis virus (LdMNPV) in Lymantria dispar asiatica. FB 28 increased the mortality at the combination with low concentration (1.6×102 IU/ml) of Btk, but FB 71 slightly reduced the mortality with middle and high concentrations (1.6×103 and 1.6×104 IU/ml) of Btk in comparison to Btk alone. Both FB 28 and FB 71 increased mortality in combination with LdMNPV at all concentrations (3×102, 3×104, and 3×106 POBs/ml) compared to LdMNPV alone. Our findings suggest that FBs enhanced pathogenic activities but depend on chemical nature of FBs.

Canine parvovirus-2 (CPV-2) has been reported worldwide as a major pathogen associated with acute hemorrhagic enteritis. The disease is a major infectious cause of death, particularly in young dogs. The earliest type of CPV-2 was replaced with three main subspecies, CPV-2a, CPV-2b, and CPV-2c, within a few years. Vaccination is carried out regularly, but the emergence of antigenic variants and the influence of maternal antibodies have limited the efficacy of commercial vaccines. New vaccines, such as the subunit vaccine, have been developed for alternative, safe, and effective vaccination. The baculovirus expression vector system (BEVS) is an excellent eukaryotic expression system with a high-level expression of foreign proteins and the ability of post-translational modification. Therefore, it is used widely to produce recombinant protein and subunit vaccines. In this study, the VP2 protein of CPV-2b cloned in the gateway vector system was generated using a baculovirus expression system in Spodoptera frugiperda (SF9) insect cells. Hemagglutination assay (HA) titers (24) were obtained, and the expression was detected in 6-His tagged VP2 and monoclonal antibody (mAb) against CPV-2 by western blotting. The VP2 protein of CPV-2b expressed in this study may provide a basis for a clinical diagnosis and vaccination applications for CPV-2.

1,2-Dichlorobenzene (1,2-DCB) is in various industries as solvents in herbicides, pesticides, and wax, and as degreasers or in the production of dyes. Studies on the hazards and risks of 1,2-DCB showed that this substance can cause skin corrosion, skin irritability, respiratory irritability, and certain target organ toxicity. Industrial workers are exposed to 1,2-DCB by inhalation or skin exposure and there is a lack of information on human hazards even though they can be exposed to organic compounds such as benzene or other DCB complexes rather than a single substance. In this study, we investigated the specific organ toxicity of 1,2- DCB and sex differences using whole-body inhalation in laboratory mice. Male and female mice were exposed to 0–120 ppm of the test substance for 13 weeks. After euthanization, the organs were collected, histopathological assessments and immunohistochemistry (IHC) were performed, and lipid peroxidation was analyzed. Macro and microscopic lesions were observed in the livers of male mice exposed to the test substance, and microscopic alterations were observed in the nasal cavities of male and female mice. IHC analysis of the liver confirmed a greater increase in cytochrome P450 induction in males than in female mice, and malondialdehyde and 4-hydroxynonenal were increased in both sexes by 1,2-DCB inhalation. Based on the relevant literature and experimental results, 1,2-DCB is believed to cause specific organ toxicity in the livers of male mice and the nasal cavities of both sexes of mice, which is supposed to be related to sex differences in cytochrome P450 induction and changes in lipid and oxidative products associated with the early metabolites of the test substance.

Hymenobacter 속(genus)은 Bacteroidota 문(phylum), Hymenobacteraceae 과(family)의 대 표 속(type genus)이다. 이 속에 속하는 세균들은 붉은색 색소를 함유하는 그람 음성 간균으로서, 자연 계의 다양한 환경에서 분리되고 있다. 본 연구에서 붉은색 색소를 함유하는 그람 음성 간균이 경남 창 원시 소재 창원대학교 교내의 연못에서 분리되었고, 이 세균은 균주 B2로 명명되었다. 균주 B2를 계통 분석 및 생화학적으로 분석한 결과, Hymenobacter 속에 속하는 것으로 밝혀졌다. 이 세균의 16S rRNA 유전자 염기서열을 genbank의 BLAST로 분석해 본 결과, 다른 어떠한 세균과도 16S rRNA 유전 자 염기서열의 상동성이 새로운 미생물로 인정되는 기준인 98.7%보다 낮은 것으로 나타났다. 균주 B2 의 지방산을 분석해 본 결과, 주된 지방산은 summed feature 3(C16:1 ω7c and/or C16:1 ω6c, 22.8%), iso-C15:0(16.2%), anteiso-C15:0(12.9%), C16:1ω5c(12.4%) 및 summed feature 4 (iso-C17:1 I/anteiso-C17:1)(9.5%)인 것으로 밝혀졌는데, 결과적으로 균주 B2의 지방산 함량은 다른 Hymenobacter 종들의 지방산 함량과 뚜렷한 차이가 있는 것을 알 수 있었다. 이 세균의 16S rRNA 유전자 염기서열 은 genbank에 accession number OQ318247로 등록되었다.

예이츠와 히니는 아일랜드의 호수와 바다에 서식하는 물고기와 어부의 이미지를 시적 글쓰기에 활용한다. 예이츠의 ｢물고기｣와 ｢어부｣는 삶의 희망과 불안을 썰물과 밀물로 탐구하며 삶의 지혜를 어부를 통해 낭만적으로 표현한다. 히니는 ｢송어｣, ｢농어｣, ｢연어잡이가 연어에게｣에서 바다와 호수의 생태적인 면과 이상 및 현실의 경계를 접목하여 해당 어류들이 사이를 치열하게 넘나든다. 히니의 물고기는 장어처럼 바다와 강의 경계를 상징하고 물과 흙 이미지의 결합인 진흙과 연결된다. 예이츠의 물고기와 어부는 경험과 지혜를 토대로 이상향을 향해 쌓아 올린 탑과 같다면, 히니의 물고기와 어부는 물과 흙의 경계에서 과거 기억과 현실, 그리고 시적 상상이 어우러져 균형을 유지하려는 수평기를 떠오르게 한다.

이 글은 예이츠와 디킨슨의 집을 프로이트의 언캐니의 관점에서 논의한다. 프로이트는 어떤 사건이나 개인의 심리적 경험인 언캐니의 개념을 단순히 신비한 것이 아니라 기묘하고 친숙하게 느껴지는 방식으로 구체화한다. 예이츠와 디킨슨에서 익숙한 집은 불안하고 섬뜩한 맥락에서 자주 마주쳐져 자아에 환원될 수 없는 불안감을 낳는다. 구체적으로 본고는 예이츠가 『연옥』에서 현실의 불안과 혼란, 절망의 상징으로서 집을 노인이 표상하는 방식, 그리고 디킨슨이 여성의 역할이 강요되고 억압되는 공간으로 제시하는 방식을 살펴본다. 그녀의 시에서 집을 매개로 자신의 과거를 은폐하고 억누르는 상황은 분열된 자아, 낯선 또 다른 나를 마주치게 한다. 예이츠와 디킨슨에게 언캐니 집은 억압되어 실현되지 못한 자아의 파편들이 잠복하여 출몰하고 의식의 경계가 모호해지는 공간이다.

Thermoelectric (TE) energy harvesting, which converts available thermal resources into electrical energy, is attracting significant attention, as it facilitates wireless and self-powered electronics. Recently, as demand for portable/wearable electronic devices and sensors increases, organic-inorganic TE films with polymeric matrix are being studied to realize flexible thermoelectric energy harvesters (f-TEHs). Here, we developed flexible organic-inorganic TE films with p-type Bi0.5Sb1.5Te3 powder and polymeric matrices such as poly(3,4-eethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) and poly (vinylidene fluoride) (PVDF). The fabricated TE films with a PEDOT:PSS matrix and 1 wt% of multi-walled carbon nanotube (MWCNT) exhibited a power factor value of 3.96 μW ‧ m-1 ‧ K-2 which is about 2.8 times higher than that of PVDF-based TE film. We also fabricated f-TEHs using both types of TE films and investigated the TE output performance. The f-TEH made of PEDOT:PSS-based TE films harvested the maximum load voltage of 3.4 mV, with a load current of 17.4 μA, and output power of 15.7 nW at a temperature difference of 25 K, whereas the f-TEH with PVDF-based TE films generated values of 0.6 mV, 3.3 μA, and 0.54 nW. This study will broaden the fields of the research on methods to improve TE efficiency and the development of flexible organic-inorganic TE films and f-TEH.