The effect of the surrounding vegetation on the seed germination and growth of mountain-cultivated ginseng (MCG, Panax ginseng C.A. Meyer) was investigated. Seed germination rate and growth were tested for allelopathy effects on four forest tree species after treatment with fallen leaves and leaf extracts. In the case of soil treatment through fallen leaves and crushed leaves, the germination rate was lower in the Quercus myrsinifolia treatments, and the average germination time was slower when Chamaecyparis obtusa was treated. In the case of Pinus densiflora and Quercus variabilis, which are used in most of the MCG cultivation areas, they did not have a significant effect on seed germination. In the fallen leaves treatments, the stem showed a tendency to lengthen. The hot water extract treatment showed a higher germination percentage than the cold water extract treatment. The extract treatment showed a deficient germination percentage of some MCG seeds. However, in the case of the treatments except for this, the germination percentage was similar to that of the control treatment. However, the Mean Germination Time, germination rate, and germination value were faster and higher than the control treatment. As a result of calculating the allelopathic index (AI) of MCG according to the extract treatment of 4 species, most had a negative effect on germination, and P. densiflora and Q. variabilis extracts showed the most significant effect. The ginsenoside content was higher in the fallen leaves treatment than in the control. The above results will help select and manage MCG plantations.

본 연구는 allelopathic potential을 지닌 벼의 교잡종을 포장에서 재배하여 그 식물체의 잎을 생물검정에 이용하여 allelopathy 효과 유무를 검정하고, 생물검정으로 선발된 교잡종을 대상으로 phenolic compounds를 조사하여 allelochemical로써의 가능성을 확인하고 나아가 phenolic compounds 생합성에 관여하는 효소의 활성 측정과 이 효소의 작용에 의해 유도되는 phenolic compounds를 구명한 결과는 다음과 같다. 1. 포장상태에서 피를 대상으로 생물검정을 실시한 결과 양친으로는 Kouketsumochi, Woo co chin yu에서 공통적으로 90% 이상의 높은 피뿌리 생장 억제율을 보였고, 교잡종에서는 Kouketsumochi/Woo co chin yu, Dongjinbyeo/Kouketsumochi, Dongjinbyeo/Woo co chin yu 조합에서 80% 이상의 높은 억제율을 나타냈다. 2. Kouketsumochi의 PAL 활성은 protein 1kg당 로 타 공시품종보다 높았다. 또한 Kouketsumochi와의 교잡종도 타교잡종보다 높은 것으로 나타났다. 3. Kouketsumochi의 cinnamate 함량은 생체중 1g당 으로서 다른품종이나 교잡종보다 2~5배 높았고, Kouketsumochi와의 교잡종도 타교잡종보다 높았다.

1. 꽃무릇으로부터 용매를 다르게 하여 잎과 인경의 MeOH와 수용추출물을 bioassay 한 결과, 농도가 5% 및 10%로 증가하였을 때는 전혀 발아되지 않았으며, 1%에서는 근장이 크게 억제되었다. 용매간의 추출효과에는 차이가 없었으나, 상추의 생장에서는 인경추출액이 잎추출액보다 억제효과가 다소 높은 것으로 보였다. 2. 꽃무릇의 인경을 추출하여 UV를 조사하였을 때 Rf 0.49-0.52에서 보인 spot는 GC-MS에 의해 lycoris alkaloid계통의 lycorenan으로 동정되었다.

서양금혼초의 군락 형성에 따른 경쟁적 우세원인을 규명하고 친환경농자재 개발을 위한 기초자료로 제공하고자 생육특성과 Allelopathy 효과를 알아보았다. 서양금혼초의 생육특성은 예초지역과 비예초지역간의 묘조(苗條) 수, 지상부 직경과 화주 수를 비교한 결과 예초지역의 묘조(苗條) 수, 지상부의 직경과 화주수가 비예초지역보다 높은 것으로 조사되었고, 서양금혼초의 수용성 추출액에 대한 레드클로버(Trifolium pratense), 들묵새(Festuca myuros), 도깨비바늘(Bidens bipinnata) 등 실험식물 14종 대해 발아와 생장 실험 결과 일반적으로 수용성 추출액의 농도가 증가함에 따라 검정식물의 발아율, 유식물의 뿌리털, shoot, root의 생장이 억제되었고 검정식물의 종류와 부위에 따라 억제정도에 있어서 차이를 보였다. 또한 서양금혼초의 수용성 추출액에 대한 실험곰팡이의 생장에 있어서는 Pythium속, Phytophthora속은 수용성 추출액의 농도가 높아짐에 따라 성장이 억제되는 경향을 보인 반면, Rhizoctonia속인 경우에는 농도가 높아짐에 따라 생장억제 현상이 뚜렷하지 않았다. 이와 같은 결과를 종합하여 보면 서양금혼초의 예초는 서양금혼초의 Split현상을 유도하여 더 많은 영양번식과 종자번식을 일으켜 군락을 형성하고, 서양금혼초에 함유된 allelochemicals에 의해 타 식물체의 발아와 생장, 미생물의 생장 등에 관여하기 때문에 제주도내 생태계에서 경쟁적 우위를 점하고 있으며, 친환경농자재로서의 활용 가능성이 있는 것으로 판단되었다.

This study was conducted to evaluate the allelopathic effect of aqueous extract of Ganghwa domestic mugwort (Artemisia spp.) on vegetables and its related allelochemicals. When the receptor vegetables, such as Chinese cabbage, lettuce, and red radish, were treated with aqueous extract obtained from Sajabalssuk (A. sp*I), Ssajuarissuk (A. sp*II) or Ssajarissuk (A. sp*III), their germination rate, leaf number, plant height, and root length were restricted with increasing concentration of aqueous extract. Allelopathic effect was the highest in radish, than lettuce and Chinese cabbage in order. The growth of topplant were more inhibited then root growth observing in restriction of plant height, root length, and chlorophyll contents. The plant height, the root length of red radish were 53.3 and 61.2% and their fresh weights were 19.8 and 26.4% compared to those of controls, respectively. A. sp*III showed the highest allelopathic effect among the donor plants. In HPLC analysis, 7 phenol compounds were identified in A. sp*I and A. sp*II, and, in A. sp*III, and hydroxybenzoic acid and phenylacetic acid were further identified as allelochemicals. It is considered that their plant growths were variously inhibited by the amounts and types of allelochemicals in aqueous extracts. To increase the productivity of farm land after cultivation of mugwort, these results can be useful to select the following field crops.

A complete diallel cross was performed to determine the inheritance of allelopathic potential and combining ability of six rice germplasm that showed different level of allelopathic potential against barnyardgrass. Parents and F1 of 30 crosses were assessed for allelophatic potential in field condition. Barnyardgrass (Echinochloa crus-galli (L.) Beauv. var. frumentaceae) was used for rice allelopathic potential indicator which was observed in height and shoot dry weight, then suppression percentage were calculated by comparing to barnyardgrass grown in control plot (no rice). It was found that general combining ability showed no significant effect, whereas specific combining ability and reciprocal effect were significant. Variance analysis confirmed that genetic control of allelopathic potential in rice against barnyardgrass was mostly directed by dominance gene effect. The dominant genes were associated with high allelopathic potential and recessive genes were associated with low allelopathic potential. Heritability in broad sense for barnyardgrass dry weight suppression was around 41%; whereas for barnyardgrass height suppression was 51%. However, heritability in narrow sense was very low. The finding suggests that accumulation of genes associated with allelopathic potential would be a strategy to develop promising varieties in reference with the specific combining ability of cross combinations.

Several wild plant species are known to contain biologically active substances that are allelopathic to weed species as well as antioxidant to foods. Plant extracts or residues from leaves of 4 species, Achyranthes japonica (speedwell), Cucumis sativus (Cucumber), Trifolium repens (white clover), and Vicia angustifolia (narrowleaf vetch) were bioassayed against Medicago sativa (alfalfa) or Echinochloa crus-galli (barnyard grass) to determine their allelopathic effects, and used for measurement of antioxidant activities. The aqueous extracts applied on filter paper significantly inhibited root growth of alfalfa. Aqueous extracts or residues from V. angustifolia showed the most inhibitory effect on alfalfa or barnyard grass seedling growth and followed by A. japonica and T. repens. Oxidative stability by Rancimat method, antioxidant activity by TBA (2-thiobarbituric acid) method and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity for the ground samples or methanol extracts were the greatest in V. angustifolia, although were less than those of commonly used antioxidants, BHT (butylated hydroxytoluene) and ascorbic acid. These results suggest that the wild plant species had potent allelopathic and antioxidant activities, and that their activities differed depending on plant species.

Alfalfa (Medicago sativa L.) plants have been reported to be autotoxic as well as allelopathic. Laboratory and greenhouse experiments through petri-dish and pot test were conducted to determine autotoxic effects of alfalfa leaf and soil extracts on the germination or early seedling growth of alfalfa, and to evaluate allelopathic effects of alfalfa leaf residues on alfalfa, barnyard grass, com, eclipta and soybean. Alfalfa seed germination was delayed depending on aqueous extract concentration, with no difference in final germination after 48 hours. Alfalfa root length was more sensitive to the autotoxic chemicals from leaf extracts than was germination or shoot length. Root growth of alfalfa was significantly inhibited at extract concentration of more than 1 g dry tissue/L (g ~textrmL-1 ). Hypocotyl growth, however, was not affected by all the concentrations of leaf extracts. Soil extracts from 4-yr-old alfalfa stand significantly reduced alfalfa root length by 66%, while soil extracts from 0,1, and 3yr-old stand stimulated root length up to 14-32% over the control. Residue incorporation with dry matters of alfalfa leaf at 100 g ~textrmkg-1 reduced seedling length of several crop and weed species, ranging from 53 to 87% inhibition. Addition of nutrient solution into alfalfa leaf extracts alleviated alfalfa autotoxic effect. This result indicates alfalfa leaf and soil extracts or residues could exert autotoxic as well as allelopathic substances into soil environments during and after establishment.

A series of aqueous extracts and residues from leaves, stems, roots, pods and seeds of Korean black soybean (Glycine max (L.) Merr.) were assayed against alfalfa (Medicago sativa) and barnyard grass (Echinochloa crus-galli) to determine their allelopathic activities through petri-dish and greenhouse experiments, and the results showed highest inhibition in the extracts or residues from the seeds, and followed by pods. The extracts of 40g dry tissue ~textrmL-1 applied on filter paper in petri-dish bioassay significantly inhibited root growth of alfalfa, and especially extracts from seeds and pods reduced root length of alfalfa more than those from leaves, stems, or roots. Plant height, root length, shoot and root dry weights of barnyard grass were reduced significantly by residue incorporation of seeds and pods as the incorporated amount increased. These results suggest that black soybean plants had herbicidal potential, and their activities were exhibited differently depending on plant parts.

Some Compositae plants are known to contain biologically active substances that are allelopathic to weeds species as well as antioxidant to foods. Aqueous extracts from leaves of 3 plant species, Cirsium japonica, Kalimeris yomena, and Lactuca saliva, were bioassayed against alfalfa (Medicago saliva) to determine their allelopathic effects. The extracts applied on filter paper in bioassay significantly inhibited root growth of alfalfa. Extracts of 20 g dry tissue ~textrmL-1 from Lactuca sativa showed the most inhibitory effect on alfalfa seedling growth and followed by Cirsium japonica and Kalimeris yomena. Oxidative stability by Rancimat method and antioxidant activity by TBA method for the ground samples were the greatest in Lactuca sativa although were less than that of a commonly used antioxidant, 1% ascorbic acid. Antioxidant activity of methanol extracts on storing meat was stably kept for 28days and was excellent compared to control. These results suggest that three Compositae plants have potent allelopathic and antioxidant effects, and that their activities differ, depending on plant species.

Oryzalexine A, a potent growth inhibitor against several weeds such as Digitaria sanguinalis (L) Scop., and Amaranthus lividus L. was purified by conventional solvent partitioning and column chromatographies. This substance showed strong inhibitory activity on several weeds: Germination of seeds of Poa annua L. was inhibited by 36.5 % at 1.0 mM and Amaranthus lividus L. by 56.1% at 2.5mM. Growth of root and shoot of Digitaria sanguinalis (L.) Scop. by 10.2% and 22.4% at 2.5mM, respectively. These suggest that Oryzalexine A in rice straw might affect the germination and growth of susceptible weeds and other plants.

This study was conducted to assess the allelopathic potentiality of Korea traditional seventy-nine rice (Oryza sativa L.) cultivars on barnyardgrass (Echinochloa crus-galli P. Beauv. var. oryzicola Ohwi) and to identify possible allelopathic compounds from selected rice cultivars, such as Seogandodobyeo, Huadobyeo and Heugbalbyeo. In the straw mixture, Seogandodobyeo showed the greatest inhibition (67.07%) on total emergence percentage. The greatest inhibition on total seedling length and dry weight of barnyardgrass occurred in Huadobyeo (58.32 %) and Heugbalbyeo (81.20%), respectively. An HPLC analysis with nine standard compounds showed that the concentrations and compositions of allelopathic compound depend upon the cultivars. Four compounds including Ο-coumaric acid in Seogandodobyeo extracts, four compounds including p-coumaric acid in Huadobyeo extracts, and seven compounds including Ο-coumaric acid in Heugbalbyeo were detected, respectively. Ο-coumaric acid (0.97 mg/g) in Seogandodobyeo, p-coumaric acid (0.92mg/g) in Huadobyeo and Ο-coumaric acid (1.02 mg/g) in Heugbalbyeo was detected as the highest amounts, respectively. The preliminary identification by HPLC analysis resulted in peaks with retention times close to those of standards which were confirmed with EI/MS. The mass spectra of p-coumaric, and Ο-coumaric acids showed molecular ions (m/z) at 164 and 164, and their base peaks (m/z) at 164 and 118, respectively.

미국자리공 및 겨자무의 잎추출물을 이용하여 기내배지상에서 몇가지 작물들과의 allelopathy를 조사한 결과는 다음과 같다. 1. 미국자리공 잎의 methanol 추출물의 농도가 증가함에 따라 공시한 모든 작물의 발아율, 근장, 배축장, 초장, 생체중, 건물중, 유묘의 활력이 급격하게 감소하였다. 2. 미국자리공의 autoallelopathy는 고농도(50u l 및 100u l) 첨가시에 나타나기 시작하였으며, 생체중과 건물중은 농도가 증가함에 따라 현저히 감소하였다. 3. 겨자무 잎의 methanol 추출물은 공시한 모든 작물의 초기생장량을 유제시키는 효과는 보였으나, 미국자리공의 잎추출물의 효과보다는 높았다. 4. 겨자무 뿌리의 methanol 추출물 처리는 밀, 미국자리공의 배축장을 농도 증가에 따라 감소시켰으나, 나머지 작물은 증가시켰다. 또한 초장도 밀, 고추, 미국자리공에서는 추출물의 농도가 증가할수록 감소하였으나 그 외의 작물에서는 약간 증가하였다. 5. 미국자리공의 methanol 추출물로부터 재출물한 4종의 분획물 중 ethyl acetate 분획물이 발아율 및 초기생육을 가장 크게 감소시켰다. 6. Ethyl acetate 분획물은 다른 분획물보다 벼, 보리, 배추의 엽록소 함량을 감소시켰다. 7. Ethyl acetate 분획물 100u l l 첨가시에 배추의 유리 proline의 함량은 31.2배 증가하였으며, 추출물농도가 증가할수록 proline 함량이 증가하였다.

알팔파 종자와 재배지 토양의 추출물이 알팔파 및 무우의 발아 및 생육에 미치는 영향과 이들 물질을 구명코자 수행한 결과를 요약하면 다음과 같다. 1) 알팔파 종자 추출물의 무우생육에는 Luna, Sparta, Magnum, Husky, Milkmaker, Challenger, Anchor의 7개 품종에서 60∼80％ 생육억제 효과를 보였다. 2) 알팔파 재배지 토양잔류추출물을 이용한 무우생육조사는 추출물의 농도가 증가하면서 발아억제 및 줄기, 뿌리 등의 생장억제에 관여하는 것으로 나타났다. 3) 알팔파 재배토양으로 알팔파와 무우 생육실험시에는 80％의 발아억제를 보이면서 줄기와 뿌리의 길이 및 생체중에서도 큰 차이를 보여 allelopathy와 autotoxicity가 관여하는 것으로 사료된다. 4) 위 실험을 통해 관여하는 물질의 활성을 분석하기 위해 조사한 결과 표준 phenol compounds와 식물부위별 추출물을 비교 분석하였더니 salicylic acid, p-hydroxybenzoic acid, vanillic acid, syringic acid, p-cou-maric acid, ferulic acid 등의 7가지 acids를 분리동정 할 수 있었다.

These experiments were conducted to determine the effects of Sorghum allelopathic substances on the callus growh of several weeds and crops. 1. When substances extracted from allelopathic Sorghum(Sorghum bicolor L.) were treated on medium, growth of callus of several weeds and crops were in-hibited. The degree of inhibition differed depending on the genotypes, ranging from 50 to 90% com-pared with that of control. 2. The extracts of above 5% Sorghum inhibited the callus growth of Che-nopodium albun L., Commelina communis L., and .Ammaranthus retroflexus L.and showed in-hibition rate of above 70% in callus growth. These results indicate that we could investigate theallelopaihy effect by using in vitro system. 3. The suitable explant for callus induction fromallelopathic plants was immature embryos, the callus induction rate differed depending on the geno-type, growth regulators and concentrations. In general, the addition of 2, 4-D and NAA onto medium increased the rate and amount of callus.