곤충 Laccifer lacca의 분비물인 stick lac에서 얻어지는 락색소는 물에 용해되는 붉은색 식용 색소로, 식품에 붉 은 색을 더하는 착색제이다. 주요 성분은 안트라퀴논계 기 반의 laccaic acid로 A, B, C, D, E로 구성된다. 본 연구 의 목적은 HPLC-DAD를 활용하여 laccaic acid를 정량적 으로 분석할 수 있는 방법을 검증하는 것과 섭취 노출량 을 산출하는 것이다. 제안된 분석법은 특이성, 선형성, LOD, LOQ, 정확성, 정확도 등 다양한 검증 항목에서 평 가되었으며, 매트릭스 효과 또한 충분히 고려되었다. 모든 매트릭스에서 검량선은 높은 선형성 (R20.999)을 보였고, 락색소의 MDL과 MQL은 각각 1.93 mg/kg와 5.86 mg/ kg로 확인되었다. 일내 및 일간 회수율 시험에서 정확도 는 91.5-100.9% 사이였고, 정밀도는 0.8-2.6%RSD로 AOAC 기준을 충족하였다. 한국에서 유통되는 다양한 식 품들을 수집하여 락색소를 모니터링하여 식품 유형별 락 색소 섭취 노출량을 평가하였다. 락색소 섭취 수준의 범 위는 국민전체, 섭취자 각각 0.0000-0.0027 mg/kg·bw/day 와 0.0000 -0.0306 mg/kg·bw/day였다. 개발된 분석법은 다 양한 식품 유형에 적용 가능하여 락색소 추출물의 효과적 인 모니터링을 가능하게 하며, 섭취량 평가를 통해 락색 소의 안전성 평가에 추가적으로 활용될 수 있을 것이다.

This study determined the minimum size of a representative molecular structure for use in future dynamic analyses of asphalt binders. The minimum representative size, considering factors such as aging, additive types, and temperature variations, was established using density and radial distribution functions. This approach ensures that the structure reflects temperature-dependent property changes, which are critical characteristics of asphalt binders. In this study, the structure of asphalt-binder molecules was generated using the composition proposed by Li and Greenfield (2014) for AAA1. To assess the appropriateness of the molecular structure size, we generated additional structures, X2 and X3, maintaining the same composition as X1, but with two and three times the number of molecules, respectively, as suggested by Li and Greenfield (2014). Silica and lignin were considered as additives, and the aging conditions examined included unaged, short-term aging, and long-term aging. In addition, 11 temperature conditions were investigated. The density and radial distribution functions were plotted and analyzed. The variables influencing the density and radial distribution functions were set as the aging degree of the asphalt binder (unaged, short-term aging, long-term aging), 11 temperature conditions ranging from 233 to 433 K in 20 K intervals, structure size (X1, X2, and X3), and the presence of additives (no additives, silica, and lignin). For density, clear differences were observed based on the degree of aging, temperature conditions, and presence of additives, whereas the structure size did not significantly affect the density. In terms of radial distribution functions, the X1 structure reflected differences based on the degree of aging and the presence of additives but was limited in exhibiting temperature-dependent variations. In contrast, the X3 structure effectively captured temperature-dependent trends, indicating that the size of the molecular structure is crucial when evaluating energy calculations or physical tensile strength, necessitating careful assessment.

Phosphine (PH3) fumigation has been widely used for controlling storedgrain insect pests, causing the development of resistance of stored-grain insect pests to phosphine. PH3 resistance in Sitophilus oryzae has been reported in Korea. However, PH3 resistance in Tribolium castaneum has not been reported yet. This study was conducted to determine susceptibilities of T. castaneum collected from five different domestic locations to PH3. The susceptibility to PH3 was investigated using the FAO fumigation method. All domestic T. castaneum individuals were controlled by PH3 at 0.04 g m-3. At 0.01 g m-3, T. castaneum collected from two domestic locations did not exhibit 100% mortality. A P45S point mutation in dihydrolipoamide dehydrogenase (dld) gene was found in a PH3-resistant strain of T. castaneum (Aus07), but not in five domestic stains or a PH3-susceptible strain (Aus10). No significant difference was found in dld or cyt-b5-r gene expression across all tested strains. However, the Gyeongju-collected strain of T. castaneum showed more than a 1.7-fold increase in cyt-b5-r expression compared to the Aus07 strain. cDNA sequence analysis revealed that P45S (C133T) in the dld gene was only present in Aus07. A characteristic single nucleotide polymorphism in the cyt-b5-r gene sequence was identified in the five domestic strains. This study suggests that it is necessary to continuously monitor PH3-susceptibility of T. castaneum in Korea to quickly identify resistant individuals and prevent the spread of PH3 resistance through rapid control.

가로수의 수종에 따른 결함 요인을 분석하고자 인천광역시의 가로수 느티나무, 백합나무, 양버즘나무 와 왕벚나무 4수종을 대상으로 시각적 수목평가를 실시하고 위해 정도를 파악하였다. 느티나무는 변재 부의 부후 상처가 다른 수종에 비해 많고 상처면적이 큰 개체도 다수 발견되나 줄기에서 관찰되는 공동 의 수는 적고 균류의 자실체 발생률 또한 낮았다. 반면 타진음 검사에서 이상 소견이 가장 많이 관찰되었 고 동일세력 줄기의 발생률과 분기지점의 결함 수관에서 차지하는 고사지의 비율 등은 다른 수종에 비 해 높다. 또한 줄기를 옥죄는 뿌리의 발생률은 비교적 낮고 수관이 차도 또는 보행로 방향으로 편중된 경향을 보였다. 백합나무는 구조적으로 안정된 수형을 이루고 있으며, 변재부의 부후상처 발생률, 타진음 검사결과 동일세력 줄기의 결함 등은 다른 수종에 비해 건전한 것으로 분석되었다. 그러나 백합나무 는 줄기가 직립하는 성질이 강하여 수관이 편향된 개체의 비율은 비교적 낮은 편이다. 양버즘나무는 세장비가 비교적 높고 줄기가 약하게 기울어 자라는 특성이 관찰되나 활관비가 높아 활력을 유지하는 것으로 판단된다. 줄기에 발생하는 결함 요인들도 비교적 낮은 수준이어서 건강성이 높으나 위험하지 않을 정도로 줄기가 기울어 수관이 차도 방향으로 편중된 개체의 비율은 높다. 왕벚나무는 세장비가 4수종 중 가장 낮아 초살이 잘 발달하는 수종이며, 활관비와 줄기의 기울기 각도는 비교적 안정한 편이 다. 그러나 부후와 공동의 발생률은 다른 수종에 비해 유의적으로 높아 상처에 취약한 수종임을 확인할 수 있다. 수종별 위험도 등급을 구분한 결과 느티나무와 왕벚나무의 결함 발생률과 위험도가 비교적 높게 나타났으며, 백합나무와 양버즘나무는 상대적으로 도로환경의 적응도가 높은 것으로 판단된다. 시각적 수목평가는 조사할 나무의 수량이 많고 선형으로 식재된 가로수의 위험성 평가에 흔히 통용되 는 방법인데, 향후 가로수의 건강한 생육과 안전한 관리를 위해 평가 항목을 개발하고 평가 기준을 표준 화하는 등의 추가적인 연구가 필요하다.

The digitization of ship environments has increased the risk of cyberattacks on ships. The smartization and automation of ships are also likely to result in cyber threats. The International Maritime Organization (IMO) has discussed the establishment of regulations at the autonomous level and has revised existing agreements by dividing autonomous ships into four stages, where stages 1 and 2 are for sailors who are boarding ships while stages 3 and 4 are for those not boarding ships. In this study, the level of a smart ship was classified into LEVELs (LVs) 1 to 3 based on the autonomous levels specified by the IMO. Furthermore, a risk assessment for smart ships at various LVs in different risk scenarios was conducted The cyber threats and vulnerabilities of smart ships were analyzed by dividing them into administrative, physical, and technical security; and mitigation measures for each security area were derived. A total of 22 cyber threats were identified for the cyber asset (target system). We inferred that the higher the level of a smart ship, the greater the hyper connectivity and the remote access to operational technology systems; consequently, the greater the attack surface. Therefore, it is necessary to apply mitigation measures using technical security controls in environments with high-level smart ships.

In ecosystems within limited resources, interspecific competition is inevitable, often leading to the competitive exclusion of inferior species. This study aims to provide foundational information for the conservation and restoration management of Microphysogobio rapidus by evaluating species’ ecological response to biological factors within its habitat. To understand this relationship, we collected food web organisms from site where M. rapidus coexist with Microphysogobio yaluensis, a specie ecologically similar to M. rapidus, and evaluated the trophic levels (TL), isotopic niche space (INS), and the overlap of INS among fishes within the habitat using stable isotope analysis. Our analysis revealed that the M. rapidus exhibited a higher TL than M. yaluensis, with TL of 2.6 and 2.4, respectively. M. yaluensis exhibited a broad INS, significantly influencing the feeding characteristics of most fish. Conversely, M. rapidus showed a narrow INS and asymmetric feeding relationships with other species, in habitats with high competition levels. This feeding characteristics of M. rapidus indicate that the increase in competitors sharing the similar resources lead to a decrease in available resources and, consequently, is expected to result in a decrease in their density.

PURPOSES : In this study, the resources and energy consumed to produce hot mix asphalt mixtures and hot mix reclaimed asphalt mixtures in asphalt concrete plants were estimated and the emissions from the detailed processes of the production process were evaluated based on TRACI(the tool for the reduction and assessment of chemical and other environmental impacts). METHODS : To estimate the energy consumption of the aggregate drying process, which consumes a significant amount of energy in the production process, an energy consumption calculation model based on the thermal equilibrium equation was used, and the energy consumed for material transportation, storage, and operation of other facilities was cited from the literature. RESULTS : For the system boundary conditions established and the inventory considered, the emissions to produce one ton of hot mix reclaimed asphalt mix are greater than the emissions to produce one ton of hot mix asphalt mix for a number of key impact categories. The process of producing hot mix reclaimed asphalt mixtures was evaluated to consume more resources and energy in the production of recycled aggregates and heating for drying than in the production of hot mix asphalt mixtures, but less resources and energy in the production of binders and natural virgin aggregates and the heating to heat these materials. CONCLUSIONS : The results of the emissions assessment using the life cycle inventory for the production of hot mix asphalt mixtures were generally similar to the results understood in the field and in much of the literatures, confirming the reliability of the methodology. However, in order to evaluate the dominance of specific processes or mixtures, it is believed that the construction of a wide range of inventory databases after inventory redesign is necessary for a specific and rigorous assessment.

Benthic attached diatoms (BADs), a major primary producer in lotic stream and river ecosystems are micro-sized organisms and require a highly magnified microscopic technique in the observation work. Thus, it is often not easy to ensure accuracy and precision in both qualitative and quantitative analyses. This study proposed a new technique applicable to improve quality control of aquatic ecosystem monitoring and assessment using BADs. In order to meet the purpose of quality control, we developed a permanent mounting slide technique which can be used for both qualitative and quantitative analyses simultaneously. We designed specimens with the combination of grid on both cover and slide glasses and compared their efficiency. As a result of observation and counting of BADs, the slide glass designed with the color-lined grid showed the highest efficiency compared to other test conditions. We expect that the method developed in this study could be effectively used to analyze BADs and contributed to improve the quality control in aquatic ecosystem health monitoring and assessment.

본 연구는 중국 요녕성 심양시 심수만(沈水灣)공원을 대상으로 여름철 주간과 야간의 소리경관 특성을 비교 분석함으로써 수변공간의 소리특성에 대한 인식도와 만족도를 평가하는 한편 이를 바탕으로 유사 공원설계시 적용할 수 있는 소리공원 설계지침을 제시할 목적으로 시도되었다. 본 연구의 핵심 연구범위는 혼하 구간으로, 데이터베이스 자원을 이용하여 GIS 플랫폼에 관련 전문 컴퓨팅 소프트웨어를 통합하고, 현지 측정 데이터와 설문조사 결과를 결합하여 도시 수변공원의 <소리경관도>를 작성하였다. 이후 이 자료를 바탕으로 시간대별 소리경관을 분석 및 해석함으로써 소리경관 변화 패턴을 분석하여 수변공원의 시간대별 소리경관 조성을 위한 전략을 제안하였다. 소리 디자인이론을 바탕으로 심양 수변공원 소리경관에 대한 특성과 문제점을 도출한 본 연구 결과가 향후 수변공간 조성을 위한 이론 및 실천적 기초자료로 제공되기를 기대한다.

Mercury and its compounds are globally managed chemicals as risks to the human body and ecosystem. It mainly enters the human body through ingestion of seafood, especially, methylmercury causes serious diseases such as central nervous system (CNS) disorder and renal dysfunction. In this study, total mercury (Hg) and methyl mercury (MeHg) were determined in seafood (16 species, n = 97) commonly consumed in Daejeon, using a gold amalgamation method. The average concentration (Hg/MeHg mean ± S.D. (minimum–maximun) mg/kg) of total Hg and MeHg in the samples was as follows; Fish 0.038 ± 0.058 (0.004 – 0.272) / 0.028 ± 0.047 (N.D. – 0.236), Crustacea 0.023 ± 0.021 (0.003 – 0.078) / 0.016 ± 0.018 (N.D. – 0.055), Mollusks 0.015 ± 0.015 (0.002 – 0.056) / 0.008 ± 0.013 (N.D. – 0.040). The concentration of MeHg in seafood were significantly correlated with total Hg concentration (p<0.001). The species with the highest average concentration of Hg was the Korean rockfish, but there was no sample that exceeded the maximum residual limit. The total %provisional tolerable weekly intake (%PTWI) value of MeHg for all of the samples was 3.76%, compared with the JECFA’s reference value, which indicates that there is almost no health risk from heavy MeHg intake through the consumption of seafood distributed in Daejeon.

PURPOSES : In a previous study, an error was detected in data pertaining to the direction and velocity of a roller. Hence, in this study, the correlation between these two variables and acceleration data is analyzed. Relevant algorithms are developed by adding variables to existing algorithms. METHODS : A tachometer and GPS are used to acquire the velocity, compaction direction of rollers, and number of compactions. Subsequently, data input to an accelerometer are compared and analyzed. RESULTS : Based on FFT analysis, it is discovered that the data are inaccurate when a forward reverse is performed. Using the GPS, the velocity of the roller is differentiated based on the number of pledges, and then added as a variable to the algorithm. Subsequently, it is evaluated and analyzed only with data during forward movement based on changes in the latitude and longitude. CONCLUSIONS : It is discovered that the acceleration data values from both the left and right rollers differ owing to their weight difference, as indicated by the asphalt gradient. Data changes based on asphalt gradients are analyzed using gyro sensors. If the correlation between the two sets of data is high, then the algorithm is advanced by introducing a cross spectrum after calibrating the acceleration value based on the gradient.

서울시 경동시장 및 인근 대형마트에서 유통 중인 건조 수산물 150여 건을 시료로, ICP-MS를 이용하여 규격 미설정 중금속인 비소, 크롬, 니켈에 의한 오염도를 확인하고 그에 따른 위해도를 분석하였다. 크롬과 니켈은 검출량 및 위해도 분석 결과, 유의할 만한 결과를 확인할 수 없었다. 비소 검출량의 경우 건조어류 1.76±1.36 (0.00- 7.34), 건조연체류 3.38±2.42 (0.06-7.89), 건조해조류 1.99±1.33 (0.28-5.97), 건조갑각류 6.62±2.50 (3.30-13.57) ppm으로, 현재 건조 농산물 일부 및 한약재에서의 3 ppm 이하 규정을 간접적으로 적용했을 때, 건조갑각류는 분석에 사용된 시료 21건 모두 기준 이상으로 검출되었고, 건조연체류는 총 20건 중 50%에 해당하는 10건이 기준 이상, 건조 어류는 총 108건 중 15건(13.9%)이 기준 초과, 건조 해조류는 35건 중 6건(17.1%)의 시료가 기준을 초과 한 결과를 보였다. 시료 건수에 따른 한계에도 불구하고, 위 결과를 통해 각 품목별 및 세부 품목별 미 규격 중금속 오염에 대한 지속적인 자료 축적과 규격 설정의 필요성을 확인할 수 있었다. 비록 %PTWI 분석 결과 아직은 안전한 것으로 확인되었지만, 중금속은 여러 경로로 인체 내로 유입되며 위해도는 총합으로써 평가해야 하므로, 해양 및 수산물의 오염으로 인한 건강 위해 가능성에 대한 확인은 다각적인 연구와 검토가 필요할 것으로 판단된다.

국토의 환경생태적 평가 등급과 환경부에서 제공하는 도시대기 측정망의 대기오염물질들 농도와의 상관관계를 분석하여 환경생태적으로 우수한 지역이 대기오염물질의 농도 저감에 영향을 미치는지를 살펴보고자 했다. 이를 위해 본 연구에서는 우리나 라 전체 260개 시군구를 대상으로 2013년부터 2018년까지 측정된 환경부 도시대기 측정망 자료와 보간법 중 하나인 IDW를 적용하여 2013년부터 2018년까지의 각 시군구별 대기오염물질 평균 농도와 환경생태적 평가 등급의 면적 비율과의 피어슨 상관분석을 수행했다. SO2, CO, NO2, PM10, PM2.5의 농도 값은 환경생태적으로 우수한 지역인 1등급의 비율이 높은 지역일수록 상대적으로 음의 상관성이 뚜렷하게 나타났다. 반대로, 환경생태적 평가등급이 낮은 5등급의 비율이 증가할수록 오히려 대기오염물질과 높은 양의 상관성을 보였다. 즉, 환경생태적으로 우수한 지역의 비율이 높을수록 대기오염물질의 농도가 낮으며, 그 반대의 경우에는 대기오염물질의 농도가 높았다. 이는 녹지 중에서도 환경생태적으로 우수한 녹지의 면적이 차지하는 비율이 높을수록 대기오염물질 의 농도 감소에 긍정적 영향을 미칠 수 있음을 보여준다. 따라서 국민의 건강에 직접적 영향을 미치는 대기오염물질의 농도를 감소시키기 위해서는 환경생태적 우수지역의 면적을 충분히 확보할 필요가 있을 것이라 판단되며, 향후 도시계획 단계에서 환경생 태 우수지역과 연계된 계획의 수립이 필요하다고 판단된다. 후속 연구에서는 이러한 오염물질들의 특성 및 환경생태적 우수지역의 패치 면적, 패치 분포 등 다양한 사항들을 고려하여 추가적인 상관분석을 수행하고, 분석 결과를 국가 정책에 반영해야 할 것으로 생각된다.

Protecting and preserving the natural and environmental treasures of Jeju Island is, eventually, the collective responsibility of the people of Jeju, all levels of the government, corporate and the environmental NGO sectors. Jeju Island has a vibrant environmental NGO sector with about 80 NGOs providing a variety of active environmental services. All efforts and services to conserve the environment of Jeju Island for the benefits of future generations need to be galvanized within the overarching environmental governance where all three sectors actively participate and make collective decisions within the three key elements of sustainable development: people, planet & profit. The key ingredient for promoting networking and collaborative opportunities between environmental NGOs, government entities and corporations is to improve their trust on one another. Given the current status of environmental NGOs - governments - corporations relations, it will take time to develop such trust, but if small measures are undertaken, mutual understanding & respect between the leaders of NGOs, government and corporations will grow and eventually result in mutual trust.

Despite the maritime industry’s significant role in the global economy, maritime accidents are a threat to life at sea and maritime economic performance. Furthermore, the human factor still accounted for as the main factor causing maritime accidents. Every year, many maritime accidents occur in Japan and Hong Kong, with collisions being the most common. In this study, Human Error Assessment and Reduction Technique (HEART) method is applied to the collisions data to identify the common mistakes committed by seafarers by determining the generic task, error-producing conditions and the value of Human Error Probability (HEP). This study aims to find the causes of collision in Japan and Hong Kong, compare them between the two countries, and apply HEART methodology to various maritime accidents. The data was sourced from the maritime accident data report of the Japan Transportation and Safety Board and the Government of the Hong Kong Special Administrative Region of the Marine Department from 2008 to 2016. There are 27 collision cases for Japan and 21 for Hong Kong. In general, human error is the most common factor leading to collisions. In conclusion, in Japan’s collision assessment, fairly simple tasks performed rapidly or with scant attention are identified as the most common generic task. However, in Hong Kong, most of the accidents occur during complex tasks. Japan has 101 EPCs for 27 cases while there are 115 EPCs for 21 cases that occurred in Hong Kong. Both Japan and Hong Kong have the time shortage, inadequate checking of progress, and poor information exchange among seafarers on the bridge as the common error-producing conditions occurred.