With medical use of hemp, many medicinal cultivars were bred worldwide. Propagating cultivar using seed has a high cost. On the other hand, vegetatively propagating cultivar has various merits including short breeding period and uniformity. This study aimed to determine optimal conditions for propagating hemp after cutting, including sterilizing of rooting media, rooting hormone, and mixing ratio of growing media and sand of rooting media. Korean landrace strain of hemp plant was grown in Wagner pot (size: 1/2,000 a) for 60 days. Its branches were cut to 70∼80 mm in length and used for cutting slips. The rooting medium, a horticultural nursery medium, was autoclaved for 3 hours at 120 ℃. The mixing ratio of sand and nursery media was 9:1, 7:3, 5:5, or 3:7 in weight. Cutting slips were coated with rooting hormone (1-naphthylacetamide 0.4%) just before planting. Cutting materials were planted in a tray pot of 72 cells and grown in a walk-in-chamber for 28 days with a temperature of 25 ℃ and an intensity of radiation of 800 μmol/㎡/sec. Seedling rates were 61.1%, 77.8%, and 63.0% for mixing ratios of 1:9, 3:7, and 5:5 of sand mixed with horticultural nursery medium. These seedling rates were significantly higher than a seedling rate of 31.5 % for a mixing ratio of 7:3. Root lengths were 97 mm, 91 mm, and 81 mm for mixing ratios of 1:9, 3:7, and 5:5 of sand mixed with horticultural nursery medium. These root lengths were significantly longer than a root length of 37 mm for a mixing ratio of 7:3. Rooting rates were 81.1 % and 91.2 % for slips coated with rooting hormone and sterilized rooting media, respectively. They were 40.0 % and 18.3 % for slips not coated with rooting hormone or sterilized rooting media, respectively. Thus, for vegetative propagating (cutting) of medicinal hemp, sterilizing of rooting media and coating slips with rooting hormone will be essential to conducting the propagation process.
This study was conducted to secure basic data for developing technologies to reduce the generation of odor substances by investigating the effects of environmental temperature on growth performance and the generation of odor substances from feces in growing pigs. A total of 16 pigs (Landrace × Yorkshire × Duroc, average body weight 56.49±0.47kg) were randomly assigned to two treatments: thermal-neutral (TN) and heat stress (HS) conditions. The experiments were conducted for two weeks, with average temperature-humidity indices of 68.91±0.09 for TN and 85.98±0.08 for HS. The results showed that HS significantly decreased average daily feed intake (ADFI, 33.3%) and average daily gain (ADG, 25.8%) compared with TN (p<0.05). Non-esterified fatty acid in serum was lower (36.2%) in HS compared with TN (p<0.05). However, protein, blood urea nitrogen, cholesterol, triglyceride, glucose, and IgG in serum showed no difference between HS and TN. Phenol (350.0%) and skatole (416.3%) were significantly higher in HS than in TN (p<0.05). The decrease in growth performance is attributed to reduction in ADFI. The increase in phenol and skatole in HS is presumed to be due to the effect of HS on the metabolism of intestinal microbial composition. Digestion rate, intestinal microbial composition, and urine emissions are known to affect odor substances. Further research on the content of odor substances in urine, nutrient digestion rate, and intestinal microbial composition is considered necessary to determine the exact associations.
The study aimed to investigate the impact of varying levels of neutral detergent-soluble fiber (NDSF) in Hanwoo growing cattle diets on rumen fermentation and methane (CH4) emissions. An in vitro rumen fermentation experiment utilized feeds with different NDSF levels, incorporating ingredients such as corn grain, soybean meal, soybean hulls, palm kernel meal, beet pulp and timothy hay. The NDSF contents in the diets were 9.02% (T1), 10.09% (T2), 12.42% (T3) and 14.63% (T4). In vitro dry matter digestibility (IVDMD) at 48 h was 7.4% higher for T4 compared to T1 (p<0.05). Total gas production at 48 h was 6.6% higher for T4 than T1 (p<0.05). CH4 production significantly decreased at 9 h and 12 h for T1 and T2 (p<0.05). At 48 h, CH4 production was 5.6% higher for T4 compared to T1 and 6.7% higher compared to T2 (p<0.05). At 12 h ans 24 h, the ammonia nitrogen concentration of T4 was approximately 33.1% and 40.4% lower, respectively, compared to T1 (p<0.05). The acetate to propionate ratio at 48 h was approximately 18.8% higher for T4 than T1 (p<0.05). From 9 h to 48 h, the proportions of butyrate and valerate were significantly higher for T4 (p<0.05). At 48 h, the dominant phylum in T4's rumen microbial community was Candidatus Thermoplasmatota Methanomassiliicoccus, an Archaea. Therefore, this study confirmed that increasing the NDSF content in growing Hanwoo cattle diets up to 12.42% increases IVDMD without increasing CH4 emissions, which is expected to positively impact Hanwoo productivity.
This study was conducted to investigate the growth characteristics of domestic 'Sulhyang' strawberry (Fragaria × ananassa Duch.) seedlings and to analyze their relationships in order to develop a growth prediction model. Fresh weight, dry weight, and leaf area were measured to validate the newly developed growth model. The relative growth rate (RGR) of ‘Sulhyang’ seedlings’ dry weight was an average of 0.026 g·g-1·d-1, and it increased to 0.066 g·g-1·d-1 on the 49th day after transplanting (DAT). The relationship between DAT and RGR was represented as RGR (dry weight)(g·g- 1·d-1) = 0.0392/(1 + exp(–(DAT – 34.9940)/5.8662)). The crop growth rate (CGR) was an average of 0.060 g·m-2·d-1, and it increased to 0.211 g·m-2·d-1 on the 70th DAT. The relationship between DAT and CGR was calculated as CGR (dry weight)(g·m-2·d-1) = 0.1293/(1 + exp(–(DAT – 49.3917)/6.0928)). The relationship between shoot fresh weight (y) and shoot dry weight (x) per plant showed a linear relationship of y = 4.3189x + 0.7812 (R2 = 0.9976). Fresh weight, dry weight, and leaf area with respect to DAT and cumulative temperature increased exponentially, and sigmoid curve models were developed based on these data. The model with the highest coefficient of determination was found for the relationship between shoot dry weight (y) and cumulative temperature (x), represented as y = 14.2285/(1 + exp(–(x – 1590.1295)/377.8112)) (R2 = 0.9715). The results of this study can be utilized as valuable information for establishing a systematic management system for seedling production using strawberry cutting propagation methods. For the development of a more precise growth prediction model in the future, it is necessary to analyze and apply a wider range of growth indicators and meteorological factors related to strawberry seedlings.
This research was conducted to analyze the relationship between the induction time of runners and the growing degree days for domestic strawberry cultivars, 'Sulhyang' and 'Arihyang'. Runners were induced at 15-day intervals over five periods, starting from late April to early June, and the study compared the number of runner formations and temperature changes for each period while it also investigated the plantlet quality on the transplanting date. Based on back calculations from the transplanting date of September 18th, 'Sulhyang' required induction from late April to early May to achieve 70-day-old plantlets and from late April to mid-May for 60-day-old plantlets. On the other hand, 'Arihyang' needed induction from late April for 70-day-old plantlets and from late April to early May for 60-day-old plantlets. To secure about 20 daughter plants, the growing degree days needed to be around 1,000°C, and a delay in runner induction led to a delay in formation of daughter plants. The relationship between growing degree days and runner formation was expressed as the following quadratic equations: for 'Sulhyang,' y = 9E-06x2 + 0.0124x – 2.4806 (R2 = 0.9696), and for 'Arihyang,' y = 1E-06x2 + 0.0192x – 2.9274 (R2 = 0.9431), and these equations showed high correlations. The crown diameter on the transplanting date was thickest in mid-May for 'Sulhyang', and it tended to increase with delayed runner induction for 'Arihyang'. In conclusion, this study confirmed that the number of daughter plants and growth characteristics varied depending on strawberry cultivars and the timing of runner inducement time. The relationship equations derived through measurements of growing degree days can be used as the fundamental data for reasonable production of daughter plants in the future.
Recently, a closed-type plant factory has been receiving attention as a advanced agricultural method. It has diverse advantages such as climate-independence, high productivity and stable year-round production. However, high energy cost caused by environmental control system is considered as a challenges of a closed-type plant factory. In order to reduce the energy cost, investigation about energy load which is directly connected to energy consumption needs to be conducted. In this study, energy load changes of a plant factory have been analytically analyzed according to the environmental changes. The target plant factory was a lettuce growing container farm. Firstly, the impact of photoperiod, set temperature and relative humidity change were examined. Under the climate condition of Daejeon in South Korea, increase of photoperiod and set temperature rose a yearly energy demand of a container farm. However, increase of set relative humidity decreased a yearly energy demand. Secondly, the climate environment effect was compared by investigating the energy demand under 9 different climate conditions. As a result, the difference between maximum and minimum value of the yearly energy demand showed 21.7%. Lastly, sensitivity analysis of each parameter (photoperiod, set temperature and relative humidity) has been suggested under 3 different climate conditions. The ratio of heating and cooling demand was varied depending on the climate, so the effect of each parameter became different.
The Republic of Korea reclaimed land to increase its food self-sufficiency rate, but the yield was reduced due to abnormal climate. In this study, it was hypothesized that rapid and continuous monitoring technology could help improve yield. Using the vegetation index (VI) analysis, the drought stress index was calculated and the drought stress for corn grown in Hwaong, Saemangeum, and Yeongsan River reclaimed tidal land was predicted according to drying treatment. The vegetation index of corn did not decrease during the last 20 days of irrigation when soil moisture rapidly decreased, but decreased rapidly during the 20 days after irrigation. The reduction rate of the vegetation index according to the drying treatment was in the order of Saemangeum>Yeongsan River>Hwaong reclaimed tidal land, and normalized difference vegetation index (NDVI) decreased by approximately 50% in all reclaimed tidal lands, confirming that drought stress occurred due to the decrease in moisture content of the leaves. In addition, structure pigment chlorophyll index (SIPI) and photochemical reflectance index (PRI), which are calculated based on changes in light use efficiency and carotenoids, were reduced; drought stress caused a decrease in light use efficiency and an increase in carotenoid content. Therefore, vegetation index analysis was confirmed to be effective in evaluating and predicting drought stress in corn growing on reclaimed tidal land corn.
본 연구는 소맥 위주 사료에 xylanase 효소제의 첨가가 육성돈의 사양성적, 영양소 소화율, 혈액성상, 분 중 휘발 성 지방산 및 암모니아성 질소 농도에 미치는 영향을 알 아보기 위하여 실시하였다. 총 192두(4처리, 8반복, 반복당 6두)의 육성돈(25.14±0.11 kg)을 공시하여 xylanase 첨가수 준(0, 0.0125, 0.025, 0.0375%)으로 6주간 사양시험을 실시 하였다. xylanase의 첨가수준이 증가함에 따라 전체 사양 구간에서의 일당증체량(ADG), 일일사료섭취량(ADFI) 및 사료요구율(FCR)이 유의적으로 개선되는 효과를 나타냈 다(p<005). 영양소 소화율에 있어서, xylanase 첨가수준이 증가함에 따라 phase Ⅰ에서는 건물 및 에너지, phase Ⅱ 에서는 조단백질 소화율이 유의적으로 개선되었으며, 또 한 육성돈의 혈중 GLU 농도는 사료 내 xylanase의 첨가 수준이 증가함에 따라 유의적으로 증가하는 효과를 보였 다(p<0.05). 반면에, 휘발성 지방산 및 암모니아성 질소 농 도에서 xylanase의 유의적인 첨가효과는 나타나지 않았다 (p>0.05). 결론적으로, 소맥 위주의 사료 내 xylanase의 첨 가는 육성돈의 사양성적, 영양소 소화율 및 혈중 GLU 농 도를 증가시키는데 긍정적인 효과를 보였으며, 육성돈 사 료내 소맥을 주원료로 사용할 경우 xylanse의 적정 첨가 수준은 0.0375%으로 사료된다.
This study was aimed to determine the effects of grow media on the mineral contents of the leaves and growth characteristics of strawberry grown under aquaponics system in a plant factory. For aquaculture, 12 fish (Cyprinus carpio) (total weight, 2.0 kg) were raised in an aquaponics tank (W 0.7 m × L 1.5 m × H 0.45 m, 472.5 L) filled with 367.5 L of water at a density of 5.44 kg·m-3 and total 34 of strawberry seedlings were transplanted in the pots filed with 200 g of orchid stone, hydroball or polyurethane sponge in the growing bed (W 0.7 m × L 1.5 m × H 0.22 m) laid out with holly acrylic sheet (140×60 mm, Ø80) on the top of the system. The pH and EC of the aquaponic solution was ranged from 7.6 to 4.9 and 0.24-0.91 dS·m-1, respectively. The concentration of NO3-N was about 28% lower than that of the hydroponic standard solution, and K, Fe and B were 10, 27 and 3.8 times lower, respectively; however, the mineral contents of strawberry leaves were in the appropriate ranges with lower contents in the leaves grown with sponge media. The organic content (OM), nitrogen (N), phosphorus (P), and potassium (K) of the sludge were 61.5, 5.72, 8.92, and 0.24%, respectively. The leaf area, leaf number, and dry and fresh weights of shoot at 81 DAT were significantly higher in the hydroball, and the average number of fruits per plant was significantly higher in both the orchid stone and hydroball. There was no significant difference in the fresh and dry weights of fruits. Integrated all the results suggest that the orchid stone and hydroball media are more effective to utilize nutrients in solid particles of aquaponic solution, compared to the polyurethane sponge.
Mushrooms in Naejangsan National Park between May and September of 2021 have been surveyed. In this period, a total of 4 divisions, 9 classes, 25 orders, 72 families, 171 genera, and 381 species, including 3 climate-sensitive biological indicator species were found. The order in which the most diverse array of species was observed is Agaricales, which includes 24 families, 64 genera, and 170 species. Among these, the genus Russula was dominant, with 30 species, followed by the genus Amanita with 27 species. Among the 12 grids we investigated, species diversity was greatest in grid F5, in which 56 species of mushrooms were found. In particular, a large number of ectomycorrhizal mushrooms, including Russula spp. and Lactarius spp. were recognized. We presume that the gentle slopes and the low occurrence of Sasa borealis in this area may create a favorable environment for wild mushrooms. In corroboration, some grids (e.g. F6, F8, and F10) covering steep slopes and harboring large numbers of Sasa borealis contained only 19 species. Based on DNA sequence analysis, the NJ21064 was identified as Chlorophyllum hortense, which is newly recorded in Korea.
The home hydroponic system used in this experiment supplied the nutrient solution using the ebb and flow-type system, and the supply time, interval, and water levels of the nutrient solutions were fixed. This experiment was conducted to find a more favorable growth medium for plant growth in the hydroponic system. The medium used in the experiment was of two types, phenolic foam and rockwool, and the heights were 28 and 38 mm, respectively. As for the the multipurpose nutrient solutions (GNU1 and GNU2) were prepared in the lab. The composition of the GNU1 and GNU2 was the same, and there was a difference between 100 and 70% in ion concentrations. The plants used were Ssamchoo (Brassica lee ssp. namai) and Romaine lettuce (Lactuca sativa L. var. longiflora). First, in the physicochemical measurements of the empty medium, change in the pH and EC of the distilled water passing through the medium was less in the rockwool than in the phenolic foam. This indicates that there is less change in the chemical properties of the nutrient solution due to the medium when rockwool is used. After hydroponic culture of Ssamchoo and Romaine lettuce, the medium showed more residual salts in Romaine lettuce. Both Ssamchoo and Romaine lettuce showed less salt accumulation in rockwool. The emergence rate was significantly higher in rockwool for both species. The lower height of the medium, the faster the emergence was. Ssamchoo displayed significantly higher fresh weight (124.7%), shoot length (107.6%), and leaf width (107.9%) when grown in GNU1, but there were no significant differences as affected by the medium. However, in Romaine lettuce, the type and height of the medium resulted in significant differences. The fresh weight of Romaine lettuce was significantly higher in rockwool (119.6%) than in phenolic foam, and significantly higher at 38 (114.6%) than 28 mm. Although there is a difference depending on the species, rockwool is more suitable for the ebb and flow-type system than phenolic foam, and the height of 38 is more advantageous than 28 mm.
본 연구에서는 ‘설향’ 딸기를 두 작기(2020-2021년, 2021 -2022년)에 걸쳐 재배하면서 외부 광환경과 생육도일온도 가 작물 생산량에 미치는 영향을 분석하였다. 2년 동안 온실 내 환경 관리, 양액 관리 등은 동일하게 하였다. 재배기간 중 주 간의 온실 온습도는 두 작기에서 유사하게 관리되었고, 야간 의 온습도는 통계적으로 차이가 있었으나 작물 생육 범위를 벗어나지 않았다. 일사량은 9월과 10월에 첫 번째 작기의 일 평균 일사량이 많아 누적일사량도 많았으며, 11월부터는 2월 까지는 두 번째 작기의 일사량, 3월에는 다시 첫 번째 작기의 일사량이 많은 것으로 나타나 1월부터의 누적일사량은 두 번 째 작기에서 많은 것으로 나타났다. 딸기의 최적 일장 조건인 8시간 이상의 일장이 나타난 일은 두 작기 간 큰 차이가 없었고, 변화 양상은 누적일사량의 변화와 유사하게 나타났다. 누 적일사량과 생육도일온도는 상관관계가 커 생육도일온도가 딸기의 생산량과 당도에 미치는 영향을 조사해 본 결과의 초 기의 누적일사량과 생육도일온도가 적었던 두 번째 작기에서 초기 수확량은 적었으나 누적일사량 및 생육도일온도가 증가 함에 따라 후기에 수확량이 첫 번째 작기보다 많았으며 잠재 적 최대 생산량도 큰 것으로 나타났다. 당도는 생육도일온도 가 증가함에 따라 감소하였으며, 이는 촉성딸기의 특성으로 판단된다. 추후 연구를 통해 단순 수확량뿐만 아니라 작물 생 육, 꽃눈분화 및 출뢰시기를 조사, 분석하여 생육도일온도가 작 물 생육에 미치는 영향을 다각도로 분석하는 연구도 필요하다 고 판단된다.
Pancreatic acinar cell carcinoma (ACC) is a rare neoplasm accounting less than 1% of malignant pancreatic tumors. A 47-year-old male patient visited the emergency room with epigastric pain. Computed tomography or magnetic resonance imaging revealed a 4.7-cm heterogeneously enhanced solid and cystic mass with internal necrosis located in the head of the pancreas. Radiological diagnosis was borderline malignancy such as neuroendocrine tumor or solid pseudopapillary neoplasm. Two months later, the necrotic mass in the pancreas head had grown up to 11 cm, compressing the duodenum, superior mesenteric vein, and proximal transverse colon. Pylorus preserving pancreatoduodenectomy with segmental resection of transverse colon was performed. Histopathological examination revealed that the tumor was pancreatic ACC. The patient recovered without any complication and was doing well without recurrence for 12 months after surgery.
This study was conducted to evaluate the forage productivity and feed value of Sasa borealis (S. borealis) using growing black goats(S. borealis) in order to improve the utilization of S. borealis and to help mitigate the problem of reduced plant species diversity caused by S. borealis in Hanlla Mountain. One control and three treatments were made by the level of addition of S. borealis silage to the TMR feed. T1(10%), T2(20%), and T3(30%) treatments showed more daily weight gains than control group. Feed conversion ratio of T2 is 4.4g, which is significantly lower than control(P<0.05). The nitrogen retention in the control, which had relatively high dry matter intake, was 12.5g, which was significantly higher than that of T3. Sasa. borealis silage is considered to be able to use as a forage source for black goats, and if it is fed in an appropriate amount, it is considered that it will help improve livestock productivity, such as weight gain and feed conversion ratio.