직물의 결점은 원단손실을 가중시키기 때문에, 검단공정에서 결점부위를 제거한다. 실제공장에서 이뤄지는 검단공정은 육안판정방식과 전자동 방식 중 하나를 채택하는데, 두 방식 나름대로 장단점이 있다. 본 연구에서는 육안판정과 전자동 방식의 장점만을 모아 검출은 사람이 하지만, 결점위치 정보 제공 및 결점정보 기록을 컴퓨터가 하도록 반자동 검단기를 개발하였다. 본 연구에서 개발한 레이저 그리드는 결점의 위치를 검단자가 쉽게 파악하도록 돕는 역할을 하며, 야드미터는 자동으로 결점의 위치를 측정하는 역할을 한다. 컴퓨터는 야드미터로 측정된 직물의 길이와 사람이 검출한 결점의 위치정보를 받아들여 저장하고 직물의 결점정보를 한눈에 보여주는 역할을 한다. 실제 사용되는 직물을 대상으로 특정 패턴으로 재단했을 때의 손실률을 계산하여 개발된 시스템의 성능을 객관적으로 평가하였다.

조사료로서 소화율을 향상시킨 신품종 형질 전환 오차드그래스를 개발할 목적으로 lignin 합성경로에 있어서 중요한 효소 유전자 중의 하나인 COMT 유전자를 cloning하여 그 특성을 해명하였다. 오차드그래스의 COMT 유전자는 식물체의 전 조직에서 발현되고 있었으며, 특히 줄기와 뿌리조직에서 높은 발현량을 나타냄으로서 목질화에 크게 관여하는 lignin 생합성 유전자일 것으로 판단되었다. Dgcomt 유전자의 발현을 억제시킨 형질전환 오차드그래스를

일몰 후 적색 및 원적색 광처리가 토마토 묘 소질에 미치는 효과를 조사하기 위해 24일간의 광처리 기간동안 12일 간격으로 묘의 초장, 제1절간장, 줄기직경을 측정하였고, 실험 마지막 날에는 묘의 뿌리와 줄기를 분리 건조하여 각 건물중을 측정하였다. 적색광 처리는 식물의 초장을 억제시키는 것으로 나타났고, 원적색광 식물의 초장신장을 촉진하는 결과를 보였다. 적색 및 원적색광 처리가 식물의 초장에 미치는 효과는 광처리 시간이 10분 또는 20분이든 큰 차이가 없었다. 또한, 적색광 처리된 식물의 줄기직경이 대조구 및 원적색광 처리된 식물의 줄기 직경보다 큰 것으로 나타났다. 적색광 처리는 원적색광 처리 및 대조구에 비해 줄기·뿌리의 건물중비가 작았다. 토마토 묘의 충실도에 미치는 광처리 효과는 적색광 처리가 가장 우수한 것으로 나타났고, 원적색광 처리된 묘의 충실도가 가장 낮았으며 광처리 없는 대조구는 그 중간정도의 충실도를 나타내었다.

Since the reform and opening up, China has maintained rapid economic growth for nearly 40 years, and is the country with the fastest economic growth in the world. However, the rapid economic growth has also paid a huge social price, which is directly related to the unreasonable economic structure and extensive growth mode. Traditional engines of economic growth began to fade. This paper through the analysis to the theory of ancient and modern Chinese and foreign economists study on economic growth: economic growth is an extremely complex process, many factors influence the economic growth, correctly grasp the role of these factors in economic growth, to grasp the law of economic growth, the correct understanding of modern economic growth is very important. Sustained economic growth in China, on the basis of dynamic analysis, combined with the current economic situation at home and abroad, as you can see, in the high-speed economic growth since reform, various factors have been attenuation or mutation, the major developed countries and regions for the adjustment of the external imbalance in pressure, the depth of the global economic growth and recovery of the factors of uncertainty, It is difficult to maintain and improve the driving force and source of China’s economic growth in the future. The current industrialization process has reached a historical and international high level and is nearing the end. The growth rate of industrial investment will slow down significantly in the future. The demographic dividend is gradually depleting, and the savings rate will gradually decrease in the future. The era of rapid economic growth brought by demographic dividend and high savings rate in a long period of time will change. Therefore, based on the perspective of supply side demand and the development of new industries, the paper analyzes the driving force of traditional economic growth in China in the past, and holds that the driving force of consumption upgrading and digital economy are the important driving force of economic growth, and strives to improve the consumption structure of residents, increase the consumption rate and vigorously develop digital economy. Shifting China’s economic growth from relying on external demand to relying on domestic demand will provide strong support for high-quality development.

A new Gomchwi cultivar ‘Damogy ’ was bred by crossing between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The selection and investigation of growth and yield characteristics were conducted from 2006 to 2011 in field and greenhouse of Highland Agriculture Research Institute, NICS, Rural Development Administration, Korea. On a newly developed cultivar ‘Damogy’, Color of petiole ear was purple, petiole trichome was exist, light of leaf back was not exist, and density of leaf vein was degree 4. Plant height, leaf length, leaf width and petiole length were 45.9, 16.9, 21.1 and 29.0 ㎝, respectively in the 3rd year in growth characteristics. Plant size was also higher than that of Gondalbi. Bolting and flowering time were Aug. 23th and Sept. 10th, respectively. In contrast, Gondalbi showed quite earlier bolting time more than 38 days compared with ‘Damogy’ and consequently earlier flowering time more than 26 day. ‘Damogy’ showed similar leaf number (129) per plant compared to ‘Gondalbi’ (130). Furthermore, yield was higher ‘Damogy’ (1,889 g/plant) than in ‘Gondalbi’ (798 g/plant). ‘Damogy’ showed higher leaf thickness (0.71 ㎜) than ‘Gonalbi’ (0.46 ㎜), and consequently showed more hardness in leaf characteristics (28.3 ㎏/㎠) compared with ‘Gondalbi’ (23.0 ㎏/㎠). ‘Damogy’ showed higher resistance in the susceptibility of powdery mildew disease compared to ‘Gondalbi’. ‘Damogy’ variety was registered plant variety protection right as a No. 89 on December 2015.

본 연구에서는 서울지역에 설치된 SK-Planet의 강우관측소에서 관측된 강우자료의 품질을 평가하고, 자료의 품질과 관측소의 공간분포를 고려하여 자료의 신뢰성 및 활용성이 검증된 최적관측소를 선정하였다. 이를 위해 SK-Planet 강우관측소에서 2013년 6월 1일부터 2014년 5월 31일까지 관측된 강우자료를 수집하여 관측소별 결측치와 이상치에 대한 통계적 특성을 분석하였다. 강우자료의 결측률은 전체 자료에 대한 것뿐만 아니라 강우발생 조건과 강우강도를 고려하여 분석하였다. 그 결과 전체 자료에 대한 결측률은 대략 20% 전후로 나타났으며, 강우 발생 시 결측률은 대략 10% 미만으로 나타났다. 이상치는 각 관측소의 총 강우량 및 인근 관측소의 강우자료를 이용하여 분석하였다. 그 결과 전체 자료에 대한 오측률은 대략 10% 전후로 나타났다. 분석 결과를 이용하여 우수한 품질의 강우자료를 관측하는 최적관측소를 선정하였다. 그 결과, 서울 지역에 설치된 전체 263개의 강우관측소 중 64개의 관측소가 최적관측소로 선정되었다. 최적관측소는 공간적으로 균질하게 분포하고 있으며, 영향범위를 고려했을 때 서울 지역을 전체적으로 포괄하는 것으로 나타났다. 본 연구에서 선정된 최적관측소의 강우자료는 서울 지역에 대한 홍수예경보 및 강우유출해석에 유용한 기초자료로 쓰일 수 있을 것이라 판단된다.

Genotyping-by-sequencing (GBS) is a robust and rapid tool to develop SNP markers. Reduced sequencing complexity and multiplex sequencing of GBS has reduced genotyping cost for complex genome. However multiplex sequencing brings low sequencing depth which can lead to reduced number of markers. Therefore to find the appropriate condition for GBS is needed. In this research we demonstrated the use of ApeKI and selective primers for GBS of pepper (Capsicum spp.). Selective primers which amplify the GBS library with one or two flanking sequences to ApeKI site were used to increase sequencing depth. By in silico digestion, we developed six different selective primers amplify 4,000-400,000 regions. We made the GBS library with eight pepper accessions in four species using six selective primers and sequenced. Proper selective primers and pooling rate for each species will be determined. This approach will be useful for genotyping Capsicum breeding lines or populations by developing high quality SNP markers.

‘MS-CDE’와 ‘VFR’의 고정된 계통을 교배하여 특성 및 수량이 우수한 F1 품종인 ‘TY알토랑’을 육성하였다. ‘TY알토랑’의 주요특성은 TMV, TYLCV, 근부위조병, 잎곰팡이병에 내병성이며, 2~3월, 7~9월에 정식하는 품종으로 숙기는 중조생종으로 평균과중 225 g 내외의 고구형의 완숙 대과종토마토이다. 경도가 강하여 저장성이 우수하고 과형이 정연하며, 착과가 안정되어 수량성이 높다. 또한 초세가 강하면서 이상줄기 발생이 적어 재배가 안정적인 품종이다.

Rice is one of the most important major food crops which provide the major food for more than half of global population. To improve the grain quality as well as grain yield has been the essential breeding goal in rice. The composition of amylopectin is the determinant of rice eating quality under certain threshold of protein content and the ratio of amylose and amylopectin. In this study, RBE 1 driven by CaMV-35S promoter was constructed and transformed using Agrobacterium tumefaciens. We selected single copy with low amylose content among transgenic lines. The mRNA expression was investigated using RT-PCR, and enzyme activity was determined using activity staining method in mid-milky stage endosperm. Also, the overexpression vectors for RBE 1 and SSS 1 driven by seed specific globulin promoter were constructed, respectively. Moreover, the RNA interference vectors for soluble starch synthase 1 and granule bound starch synthase 1 derived by CaMV35S promoter were constructed, respectively and transformed using Agrobacterium tumefaciens. The transgene has been confirmed by amplification of HPT and target gene. The transgenic plants obtained will be used to investigate the gene function of related starch pathway in plant cells using Gopumbyeo as a wild type rice, based on the gain-of-function and the loss-of-function. The development of designed site-specific endonucleases boosted the establishment of gene targeting (GT) techniques in a row of different species. However, the methods described in plants require a highly efficient transformation and regeneration procedure and, therefore, can be applied to very few species. Here, we describe a highly efficient GT system that is suitable for all transformable plants regardless of transformation efficiency. Efficient in planta GT was achieved in rice by expression of a site specific endonuclease (SSS1::ZFN) that not only cuts within the target but also the chromosomal transgenic donor, leading to an excised targeting vector.

The purposes of this research project are to identify quantitative trait loci (QTLs) associated with yield-related traits using a recombinant inbred line (RIL) population derived from a cross between a high-yield soybean genotype SS0404-T5-76 and Daewonkong and to develop high-yield soybean and lodging-resistant sprout soybean cultivars. For development of DNA markers and identification of functional sequence variations, firstly, whole genome of five soybean genotypes, Sinpaldalkong 2, SS2-2, Pungsanamulkong, SS0404-T5-76 and Daewongkong, were sequenced using Illumina Hi-Seq technology. SS2-2 is a EMS-induced mutant of Sinpadalkong 2. SS0404-T5-76 showing high-yield is a F8 RIL derived from a cross of Pungsanamulkong x SS2-2. Daewonkong is a elite cultivar with high-protein. Furthermore, to construct a genetic linkage map, we are advancing F4 lines of SS0404-T5-76 x Daewonkong by single seed-descent. Secondly, we developed high-protein and high-yield soybean lines and lodging-resistant sprout lines. Area-adaptability tests of these promising lines are performing in three different locations including Jeju, Naju, and Suwon. Based on the results of area adaptability tests, we are planing to conduct cultivar registration of the promising soybean lines.

Eating quality is critical for consumers who take rice as staple food. Here we present the development and identification of high eating quality rice lines. The identification of positive transgenic lines, physicochemical properties of transgenic rice, mRNA expression and enzyme activity were analyzed. OsSbe1 was introduced into Gopumbyeo seeds using Agrobacterium-mediated transformation, and 1,005 out of 1,065 T1 plants were shown positive. The apparent amylose contents in T2 brown rice ranged from 11% to 25% in 890 favorable lines (Gopumbyeo was used as a reference with 18% of AAC). The activity of starch branching enzyme including three isoforms (SBE1, SBE3, and SBE4) in endosperms of T3 lines was higher than that of Gopumbyeo. Physicochemical properties related to eating quality for T3 polished rice were detected using 52 favorable lines out of 500 lines selected according to AAC. The Toyo taste meter value in 52 T3 lines ranged from 61.1 to 72.6, whereas 70.4 in Gopumbyeo. Of them, eleven lines displayed the higher palatability score than Gopumbyeo. Moreover, these elite lines produced higher yields (607.9~695.8 kg/10a) than Gopumbyeo (602.7 kg/10a). These results indicated the possibility of developing new high quality rice varieties in the future.

To develop natural antimicrobial agents for keeping qualities of postharvested greenhouse produce the antimiocrobial actions of Polygonum cuspidatum Sieb. et Zucc. extract , which showed remarkable antimicrobial effects against microorganism causing the postharvest decay of greenhouse produce, were investigate. In the inhibitory experiment of enzymes related to energy production metabolism hexokinase activities decreased to 73% and 68% by treating with Polygonum cuspidatum Sieb. et.Zucc. extract and Eugenia caryophyllata Thumnberg extract in comparison with control, respectively. Direct visualization of microbial cells by using both transmission electron microscope and scanning electron microscope showed that microbial cell membrane was destroyed by treating with the dilute extract solution. this change of celluloar membrane permeability could be identified in the experiment that 0-nitrophenyl--D-galactopyrano-side(ONPG), the artificial substrate of -galactosidase, was hydrolyzed in the presence of the extract, indicating that the membrane was perturbed. The separation and identification of the most antimicrobialo substances isolated from Polygonum cuspidatum Sieb et. Zucc. extract and Eugenia caryophyllata Thunberg extract were carried out by using gas chromatography and mass spectrometry 9GC/MSD), which were identified as eugenol. As a result, the functionality of Polygonum cuspidatum Sieb. et Zucc. extract and Eugenia caryophyllata Thunberg extract as antimicrobial agents for keeping qualities of postharvested greenhouse produce may be recommended.

본 실험은 고품질 다수성 생강 품종육성에 필요한 기초 자료를 얻기위해 국내에서 수집한 우량생강 24클론을 포장과 하우스에서 재배하여 주요 작물학적 특성을 조사한 결과 다음과 같다. 조사된 클론중에서 수량과 6-gingerol 함량에서 높은 유의차가 인정되었다. 포장에서는 CG9579(3520kg/10a)의 수량이 가장 높았고, 그 다음으로 CG95134, CG9512, CG96109, CG9596 순이었다. 우량 클론으로 선발된 클론중 6-gingerol 함량을 조사한 결과 CG9579가 4.44mg/g로 가장 높았으며, 그 다음으로는 CG9583이 4.35mg/g, CG95134가 4.32mg/g순이었다. 하우스에서 는 수량이 CG9503과 CG9521 클론이 10a당 5529kg으로 가장 높았고, CG9515, CG9578, CG9579, CG96103 그리고 CG95105 클론이 10a당 4,000kg이상의 수량을 보았다.