This study evaluated the efficacy of a wild boar repellent (Repellent A) consisting of tannins and plant oils (castor oil, garlic oil, and cinnamon oil). Sixty farmed wild boars (4-8 months old) were divided into three groups: the normal control group (NC, n=20), the experimental group (EP, n=20), and the comparative experimental group (C-EP, n=20), which used Repellent B consisting of guaiacol, eugenol, menthol, thymol, and indole. EP and C-EP were equipped with four repellents per feeder, while no repellents were installed in the NC feeder. The feed intake and the number of feeding approaches were measured for one week in all groups. The number of approach of wild boars in feeders was monitored daily using a CCTV camera. The daily feed intake per farmed wild boar in EP and C-EP was significantly decreased compared to NC (p<0.05), and EP was significantly decreased compared to C-EP (p<0.05). In the average number of daily approaches, EP and C-EP were significantly decreased compared to NC (p<0.05), and EP was significantly decreased compared to C-EP (p<0.05). In conclusion, Product A has been confirmed to have excellent repellent effects on wild boars, and it could be used to prevent wild boars from approaching pig farms.

The bean bug, Riptortus pedestris, is one of major hemipteran pests against the soybean, Glycine max in Korea. In previous study, it was suggested that water-taking in the first instar nymphal stage of R. pedestris is certainly necessary for successful ecdysis and their survival. To investigate water-taking behavior of the first instar nymphs of R. pedestris on plant, electrical penetration graphic patterns were analysed while R. pedestris fed on the napa cabbage leaf, Brassica rapa subsp. pekinensis by using EPG system. In the result, a X-waveform was dominantly observed for average 42.0 min out of total recording time (120 min). There was significant positive correlation (r = 0.457, P = 0.000452, n = 55) between body weight increase and cumulative durations of X-waveform. In addition, histological observation of cross-sections indicated that stylet tip in the plant tissue during X-waveform period is located nearby xylem cells, suggesting that X-waveform is closely related to xylem sap feeding behavior of R. pedestris in plant.

산림 생태계에서 초식 곤충은 생물 다양성의 구조와 기능에서 주요한 역할을 담당하는데 알려져 있는 곤충 종 수의 25%를 차지할 정도로 중요한 부분이다. 초식 곤충 종 풍부도와 다양성은 식물 종 다양성과 밀접한 연관을 지니고 있다. 이 연구에서는 우리나라 온대림에 서식하는 초식 곤충 활동량을 나타내는 식흔량이 먹이식물 다양성, 균등도, 나뭇잎 수, 먹이식물 밀도와 관련된 기저단면적 (basal area), 흉고직경 (DBH)과 어떠한 관련이 있는가를 알고자 하였다. 지리산국립공원 온대낙엽수림에 0.1 ha의 방형구를 두 군데 설치하여 잎을 씹어먹는 초식 곤충 (chewer)에 의한 식흔량을 측정하였다. 연구결과 평균 11.2% (±1.76%)의 식흔 발생량을 확인하였고, 조사 지역 중 식물 다양성이 높은 곳보다 낮은 곳에서 식흔량이 더 많았다. 또한 한국 남부 온대림에서 초식 곤충 활동에는 밀도 요인인 기저단면적이 긍정적으로 작용하는 것을 알 수 있었다. 추후 온대 낙엽활엽수림의 영양 단계 (trophic level)에서 초식 곤충의 여러 길드가 어떤 작용으로 영향을 받는가에 대한 연구가 필요하다고 생각한다.

RNA interference (RNAi)-based strategy has been developed to control various phytophagous chewing pests. However, only a few cases of RNAi-based control success have been reported for sucking pests, suggesting that sucking pests likely ingest less amount of transgenic subcellular hairpin RNA (or dsRNA). In this study, as the basic information for the establishment of ingestion RNAi against sucking pests, feeding amount and time course of plant subcellular fractions of the four sucking pest species (Frankliniella occidentalis, Frankliniella intonsa, Tetranychus urticae and Nilaparvata lugans) were determined by quantitative PCR (qPCR). Adults of the four species were starved for 24 h and then fed with kidney bean leaf (F. occidentalis, F. intonsa, T. urticae) or rice leaf (N. lugens) for 48 h. The leaf-fed adults were collected every 6-h interval and their genomic DNA was extracted. The ingested fractions of chloroplast and nuclear were quantified using rubisco and 50s rRNA as marker genes, respectively. The ingested amount of rubisco and 50s rRNA genes in F. occidentalis, F. intonsa and T. urticae showed rapid increasing pattern after feeding and then slightly reduced over time. In contrast, N. lugens neither ingest nuclear nor showed any distinct feeding pattern of chloroplast. These results demonstrate that F. occidentalis, F. intonsa and T. urticae ingest both chloroplasts and nucleus along with cytosol as cell-feeders but N. lugens, a phloem sap feeder, does not ingest nucleus during sucking. Our findings further suggest that ingestion RNAi-based control strategy would work better for cell-feeding sucking pests compared to phloem sap-feeding sucking pests.

귤나무속 과수의 주요 해충인 귤응애의 방제효과 극대화를 위해 괭이밥의 천적유지식물 적용 가능성을 검토하였다. 괭이밥을 지피식물로 조성한 처리구에서 일반 초생재배구에 비해 포식성 응애류 밀도가 평균 3배 이상 높게 나타났으며, 제초구에 비해 3.7배 낮은 귤응애 발생 밀도를 확인 할 수 있었다. 칠레이리응애는 괭이밥응애 알과 성충을 포식하지 못하였고, 사막이리응애는 일일 평균 괭이밥응애 알 1.96개와 암컷 성충 1.93개를 포식하였다. 온도 23 ± 1℃, 습도 70 ± 5%, 광조건 16L : 8D로 설정된 사육실에서 괭이밥응애 알에서 성충까지 17.4일이 소요되었으 며 77.5%의 부화율과 84.8%의 유충생존율을 확인하였다.

담배가루이 등 방제용 천적으로 유망한 담배장님노린재(Nesidiocoris tenuis)의 줄알락명나방(Ephestia kuehniella) 알 섭취 전 먹은 식물성 먹이에 따라 줄알락명나방알 섭취량이 달라지는지를 알아보기 위해 포트에 재식된 참깨, 파프리카, 솜에 적신 물을 각각 하루 동안 섭식하게 한 담배장님노린재에게 냉동한 줄알락명나방의 알을 공급하고 하루 동안 섭취한 알의 개수를 비교하였다. 파프리카를 먼저 섭식한 담배장님노린재가 가장 많은 55.7±30.19개의 줄알락명나방 알을 섭취한 것으로 나타났으며, 참깨를 먼저 섭식한 경우에는 줄알락명나방 알을 24.2±34.70개 섭취하였고, 물을 먼저 섭식한 경우에는 줄알락명나방 알을 49.2±30.58개 섭취하였다. 파프리카와 물을 먼저 섭식한 경우 통계적인 유의차는 보이지 않았다. 그러나 참깨를 먼저 섭식한 경우 파프리카 또는 물을 먼저 섭식한 경우에 비해 더 적은 수의 줄알락명나방의 알을 섭취한 것으로 나타났다.

This study was carried out to know whether the host plant feeding have an influence to subsequent prey consumption of a zoophytophagous mirid, Nesidiocoris tenuis, with biological control potential. Piece of leave of Paprika, Sesame, and cotton ball soaked with water were respectively provided to the test insects, adult mirid starved about a day, and frozen eggs of Ephestia kuehniella, were presented to them for a day and counted the number of the eggs consumed by them. The mirid fed by Sesame leave took significantly less prey than both the Paprika and the water fed one.

We present the first comprehensive cladistic analysis of Miridae, the plant bugs, based on analysis of 3935 base pairs of mitochondrial (16S, COI) and nuclear (18S, 28SD3) DNA for 91 taxa in seven subfamilies. We analyzed the data using maximum likelihood (ML), maximum parsimony (MP), and Bayesian inference (BI) phylogenetic frameworks. A Bayesian relaxed molecular clock was used to examine divergence times, and ancestral feeding habits were reconstructed using parsimony and a Bayesian approach. Clades recovered in all analyses are as follows: Cimicomorpha, Miroidea, and Miridae; Bryocorinae: Bryocorini; Stenodemini; Mirinae; Deraeocorinae (Clevinemini + Deraeocorini); Cylapinae; Isometopinae; Bryocorinae: Dicyphini; Orthotylini; Phylinae (Phylini + Pilophorini), and Phylinae is sister-group to all the remaining mirid taxa. These results are largely congruent with former hypotheses based on morphological data with respect to the monophyly of various subfamilies and tribes however, our results indicate that the subfamily Bryocorinae is not a monophyletic group as the two tribes, Dicypini and Bryocorini, were separated in our phylogeny. Ancestral feeding state reconstructions based on Bayesian and parsimony inference were largely congruent and both reconstructed phytophagy at the root of the Miridae.

복숭아혹진딧물의 섭식행동을 관찰하기 위해, 고추, 무, 배추, 가지, 참외 등 5종류의 기주식물에서 EPG기술을 이용하였다. 복숭아혹진딧물의 구침이 식물체 표면에 접촉된 후 전기적 연결이 일어나기까지 소요되는 시간과 맨 처음 potential drop이 일어나기까지 소요된 시간, 구침의 세포간극내에서의 활동에 의하여 일어나는 potential drop의 수, 전기적 연결신호가 나타난 시점부터 체관부를 섭식하는 순간까지 소요된 시간 등을 조사한 결과 Potential drop 수에서는 기주들 사이에 유의성 있는 차이를 나타내었다. 그러나 나머지 요인에서는 기주식물들 사이에 큰 차이를 보이지 않았지만, 참외나 배추보다는 고추, 무, 가지에서 즘 더 기주선호성과 관련된 섭식패턴들을 확인할 수 있었다.

From a chemist's perspective, Scarabaeidae is one of the most exciting group of insects to work on the isolation, identification, and synthesis of sex pheromones because-as opposed to Lepidoptera, which by and large utilizes straight chain alcohols, aldehydes, and acetates-the pheromonal chemistry of scarab beetles is remarkably diverse. While species in the subfamily Rutelinae utilize pheromone constituents, which are presumably fatty acid derivatives, the more primitive species in the subfamily Melolonthinae use phenolic, amino acid derivative, and terpenoid compounds. Here, I discuss the recent advances we have accomplished in the identification of scarab sex pheromones with especial emphasis on their chemical diversity. Also, I discuss the potential role of these sex pheromones in insect pest management. Field tests revealed that, in contrast to what has been frequently observed in the Lepidoptera, the higher the dosage of sex pheromone loaded in the traps the greater the capture of scarab beetles. These data suggest that mass trapping is more likely to be useful for scarab pest management than mating distruption.

본 실험은 재식밀도와 재식양식이 Sorghum-Sudangrass hybrid(sordan 79)의 생육특성, 건물수량, 조단백질수량, 기호성 등에 미치는 영향을 알아보고자 실시하였으며 그 결과를 요약하면 다음과 같다.1. 재식밀도가 높아짐에 따라 초장, 엽장, 엽폭(P<0.05), 엽수는 대체적으로 감소하였고, 같은 재식밀도에 있어서는 직사각형구에서 증가하였다.2. 1차 예취시 엽비솔은 밀식구중 직사각형구(30kg/ha, 25cm×4cm)