나리(Lilium spp.)는 절화, 정원 식물 및 화분 식물과 같은 관상용 가치로 인해 가장 중요한 화훼 작물 중 하나이다. 나 리는 연작으로 인한 환경 스트레스에 민감하며, 환경 스트레 스의 원인 중 하나로는 염 스트레스가 있다. 본 연구는 분홍 색 오리엔탈 나리 'Medusa', 'Lake Carey', 'Ovada'의 생 육 시기별 염스트레스에 따른 표현형 및 색 관련 화합물 함 량 변화를 조사하였다. 염 처리는 생육 시기에 따라 다양한 처리기간(무처리, 발아 전, 발아 후, 전체 생육기간)에 주 1 회 염(8dS･m-1)처리를 실시하였다. 생육 시기별 염스트레스 에 의한 개화의 차이가 있었지만, 전체 생육기간동안 염 스 트레스 처리시 모든 품종에서 개화가 이루어지지 않았다. 염 스트레스 처리 시기에 따라 초장과 꽃의 크기가 감소율이 달 랐으며 'Medusa', 'Lake Carey'는 발아 후 염 처리에서 정 상 개화하였다. 또한, 염스트레스는 꽃과 같은 식물에서 생성 되는 색 관련 화합물인 페놀과 플라보노이드 함량도 시기별 로 차이가 있었다. 품종마다 차이는 있지만, 발아 전이 발아 후 염 처리보다 총 페놀과 총 플라보노이드 함량이 더 낮은 것을 확인하였다. 이 결과는 생육 시기에 따라 염 스트레스 에 의한 나리의 표현형과 화색 관련 화합물의 함량의 변화에 차이가 있었으며 생육초기 염스트레스에 의한 피해가 높은 것으로 판단된다.

An austenitic stainless steel canister functions as a containment barrier for spent nuclear fuel and radioactive materials. The canister on the spent fuel storage system near the coastal area has several welding lines in the wall and lid, which have high residual tensile stresses after welding procedure. Interaction between austenitic stainless steel and chloride environment from a sea forms a detrimental condition causing chloride induced stress corrosion cracking (CISCC) in the canister. The South Korea is concerned with the dry storage of high-level spent nuclear fuel and radioactive wastes to be built on the site of a nuclear power plant. The importance of aging management has recently emerged for mitigating CISCC of dry storage canisters. When a corrosive pit is created by a localized corrosion in a sea water atmosphere, it initiates and grows as CISCC crack. Surface stress improvement works by inducing plastic strain which results in elastic relaxation that generates residual compressive stress. Surface stress improvement methods such as roller burnishing process can effectively mitigate the potential for CISCC of the canister external surfaces. The generation of compressive stress layer can inhibit the transition to cracking initiation. In this study, a flat roller burnishing process was applied as a prevention technology to CISCC of stainless steel canisters. Roller burnishing process parameters have been selected for 1:3 scale canister model having a diameter of 600 mm, a length of 1,000 mm and a thickness of 10 mm on the basis of the burnishing conditions available to control residual tensile stress of austenitic stainless steel plate specimens. The surface roughness of the scaled canister model was investigated using a surface roughness measurement equipment after roller burnishing treatment. The surface residual stresses of the scaled canister model were measured by a hole drilling contour method attached with strain gauge. The burnishing test results showed that the surface roughness of the scaled canister model was considerably improved with flat rollers having the tip width of 4 mm. The surface of the scaled canister model had significant residual compressive stress after burnishing treatment. The roller burnished canister with good surface roughness could reduce the number of crack initiation sites and the residual compressive stress formed on the welded surface might prevent the crack initiation by reducing tensile residual stress in the weld zone, finally leads to CISCC resistance.

The spent fuel storage canister is generally made of austenitic stainless-steel and has the role of an important barrier to encapsulate spent fuels and radioactive materials. Canister near coastal area has welding lines, which have high residual tensile stresses after welding process. Interaction between austenitic stainless steel and chloride environment forms detrimental condition causing chloride induced stress corrosion cracking (CISCC) in canister. Reducing or eliminating tensile stress on canister can significantly decrease probability of crack initiation. Surface stress improvement works by inducing plastic strain which results in elastic relaxation that generates compressive stresses. Surface stress improvement methods such as burnishing process can effectively prevent for CISCC of canister surfaces. In this study, burnishing treatment has been evaluated to control residual tensile stress practically applicable to atmospheric CISCC for aging management of steel canisters. Burnishing process was selected as a prevention technology to CISCC of stainless steel canisters to improve resistance of CISCC through enhancement of surface roughness and generation of compressive residual stress. SUS 316 SAW (Submerged Arc Welding) specimens were burnished with flat roller and round roller after manufactured and assembled on CNC machine using base plate. The burnishing test results showed that the surface roughness of SUS 316 SAW welded specimens after roller burnishing of pass No. 5 was improved with 85% with flat roller and 93% with round roller, individually. Surface roughness showed the best state when burnished at pressure of 115 kgf, feeding rate of 40 m/stroke and pass No. of 5 turns with round roller. The surface of SUS 316 SAW welded specimens had much high residual compressive stress than yield stress of SUS 316 materials with roller burnishing treatment, independently of kinds of roller. The surface of the welded specimen by round roller burnishing showed smaller compressive stress and deeper stress region than in the surface of flat round roller burnishing. The roller burnished canister with good surface roughness could reduce the number of crack initiation sites and the high residual compressive stress formed on the welded surface might prevent the crack initiation by reducing or eliminating tensile residual stress in the weld zone, finally leads to excellent CISCC resistance. The crack growth behavior of SUS 316 welded specimens will need to investigate to evaluate the corrosion integrity of the canister materials under chloride atmosphere according to burnishing treatment.

In this study, an AISI 316 L alloy was manufactured using a selective laser melting (SLM) process. The tensile and impact toughness properties of the SLM AISI 316 L alloy were examined. In addition, stress relieving heat treatment (650oC / 2 h) was performed on the as-built SLM alloy to investigate the effects of heat treatment on the mechanical properties. In the as-built SLM AISI 316 L alloy, cellular dendrite and molten pool structures were observed. Although the molten pool did not disappear following heat treatment, EBSD KAM analytical results confirmed that the fractions of the low- and high-angle boundaries decreased and increased, respectively. As the heat treatment was performed, the yield strength decreased, but the tensile strength and elongation increased only slightly. Impact toughness results revealed that the impact energy increased by 33.5% when heat treatment was applied. The deformation behavior of the SLM AISI 316 L alloy was also examined in relation to the microstructure through analyses of the tensile and impact fracture surfaces.

본 연구는 케일(Brassica oleracea L. var. acephala)의 노지 재배 시, 생육 초기에 노출될 수 있는 건조 스트레스 조건에서 Glutamate (Glu)의 스트레스 경감 및 생육 회복 효과를 구명하기 위해 실시하였다. 건조 조건(D)에 노출된 케일에 5㎛ Glu 용액을 경엽처리하였다(D+Glu). Glu 처리 전 및 처리 후 4, 8일 차(0, 4, 8DAT)의 생육과 엽록소 함량을 측정하고 식물 체내의 ABA와 Glu, Pro의 함량을 분석하여 정상 환경 및 D, D+Glu 처리구 간의 비교를 실시하였다. 엽록소 함량의 경우, 8DAT에서 D+Glu 처리구는 일정한 수준을 유지한 반면에, D처리구는 14% 감소하였다. 엽면적으로 대표되는 생장 또한 D+Glu 처리구가 D 처리구에 비해 높게 나타났다. 또한 4, 8일차의 ABA 함량이 D 처리구에서 D+Glu 처리구에 비해 44.13, 49.18% 높게 나타났으며, 체내 아미노산 대사 및 건조저항성 지표인 Glu, Pro 함량은 D 처리구에 비해 D+Glu 처리구에서 보다 높은 수준을 유지하였다. 이러한 결과를 통해, Glu 처리에 의해 건조 스트레스가 경감되고 생육이 회복될 수 있다고 판단된다.

In this study, the effects of cryogenic treatment cycles on the residual stress and mechanical properties of 7075 aluminum alloy (Al7075) samples, in the form of a tube-shaped product with a diameter of 500 nm, were investigated. Samples were first subjected to solution treatment at 470˚C, followed by cryogenic treatment and aging treatment. The residual stress and mechanical properties of the samples were systematically characterized. Residual stress was measured with a cutting method using strain gauges attached on the surface of the samples; in addition, tensile strength and Vickers hardness tests were performed. The detailed microstructure of the samples was investigated by transmission electron microscopy. Results showed that samples with 85 % relief in residual stress and 8% increase in tensile strength were achieved after undergoing three cycles of cryogenic treatments; this is in contrast to the samples processed by conventional solution treatment and natural aging (T4). The major reasons for the smaller residual stress and relatively high tensile strength for the samples fabricated by cryogenic treatment are the formation of very small-sized precipitates and the relaxation of residual stress during the low temperature process in uphill quenching. In addition, samples subjected to three cycles of cryogenic treatment demonstrated much lower residual stress than, and similar tensile strength compared to, those samples subjected to one cycle of cryogenic treatment or artificial aging treatment.

고주파 처리는 파밤나방(Spodoptera exigua)의 생리변화를 유발시켜 섭식행동, 발육 및 면역반응의 변화를 초래한다. 본 연구는 이러한 고주파의 영향을 파밤나방의 생화학적 변화를 통해 분석했다. 고주파(5,000 Hz, 95 dB) 처리는 중장 상피세포의 단백질 합성과 분비를 억제시켰다. 또한 이 고주파 처리는 중장의 인지질분해(phospholipase ) 소화효소의 활성을 현격하게 억제시켰다. 고주파 처리는 세 종류의 열충격단백질과 지질운송단백질(apolipophorin III)의 유전자 발현을 변동시켰고 이러한 변화는 중장 조직에서 뚜렷했다. 혈림프 혈장에 존재하는 지질 및 유리당의 함량이 고주파 처리에 의해 현격하게 증가했다. 이러한 결과는 고주파 처리가 파밤나방의 체내 생화학적 변화를 유발시켜 생리적 교란을 유도하는 스트레스로 작용한다는 것을 제시하고 있다.

The key regulators of apoptosis are the interacting protein of the Bcl-2 family. Bcl-2, an important member of this family, blocks cytochrome C release by sequestering pro-apoptotic BH3-only proteins such as Bid, Bad, Bax and Bim. The pro-survival family members (Bcl-2, Bcl-XL, Bcl-W) are critical for cell survival, since loss of any of them causes cell death in certain cell type. However, its role during early porcine embryonic development is not sufficient. In this study, we traced the effects of Bcl-2 inhibitor, ABT-737, on early porcine embryonic development. We also investigated several indicators of developmental potential, including gene expression (apoptosis-related genes) and apoptosis, which are affected by ABT-737. Porcine embryos were cultured in the PZM-3 medium with or without ABT-737 for 6 days. In result, significant differences in developmental potential were detected between the embryos that were cultured with or without ABT-737 (14.7±3.0 vs 30.3±4.8%, p<0.05). TUNEL assay showed that the number of containing fragmented DNA at the blastocyst stage increased in the ABT-737 treated group compared with control (4.7 vs 3.7, p<0.05). The mRNA expression of the pro-apoptotic gene Bax increased in ABT-737 treated group (p<0.05), whereas expressions of the anti-apoptotic Bcl-2 family members (Bcl-2, Bcl-XL, Bcl-W) decreased (p<0.05). Also, expressions of the ER stress indicator genes (GRP78, XBP-1 and sXBP-1) increased in ABT-737 treated group (p<0.05). In conclusion, Bcl-2 is closely associated with of apoptosis- and ER stress-related genes expressions and developmental potential in pig embryos.

This studies were carried out in summer season to increase high temperature tolerance using hydrogen peroxide treatments on cucumber in greenhouse. The water stress of cucumber in greenhouse by the hydrogen peroxide treatments showed as control〉250 mM〉500 mM treatments in order. The photosynthesis rate of cucumber at 30℃ did not show difference with each hydrogen peroxide treatment in temperature controlled greenhouse. However, the photosynthesis rate of cucumber in the control and hydrogen peroxide treatments at 40℃ was significantly different. The photosynthesis rate of cucumber in combined treatment with 1,000 mg·L-1 CO2 supply and hydrogen peroxide was also higher than control, however, there was no different of photosynthesis in 250 mM and 500 mM treatment. The value of Fv/Fm and Fm/Fo of chlorophyll fluorescent in 500 mM hydrogen peroxide treatment at 40℃ was highest. Also the activity of POD, the antioxidant enzyme, was higher with high hydrogen peroxide concentration than the other treatments. The high temperature limits for growth were 43℃ in the control, 44℃ in the 250 mM and 46℃ in the 500 mM according to analyze chlorophyll fluorescent Fo. The high temperature tolerance in cucumber increased approximately 3℃ by the hydrogen peroxide treatments under this experiment conditions.

7050 Al합금의 응력부식저항성에 미치는 2단 시효처리의 영향을 미세조직관찰, 전기전도도시험 및 SCF값을 평가함으로써 조사하였다. 최대 경도 이상 과시효에 의해 주 강화상인 η'상이 η상으로 변태되었고, 입내 및 입계에 존재하는 η상의 크기와 간격이 커졌으며 그 결과 응력부식저항성이 증대되었다. 2차 시효시간의 증가에 따라 전기전도도값은 증가되었으나 항복강도의 감소로 인하여 SCF값은 감소되었는데, 이러한 결과는 응력부식저항성의 증가를 의미한다. AMS 4107규격을 기준으로 하였을 때 7050 Al합금 단조재의 적정시효조건은 1차시효가 120˚C에서 6시간, 2차시효는 175˚C에서 12시간이었다.

We investigate the effect of endoplasmic reticulum (ER) stress inhibitor treatment during parthenogenetic activation of oocytes on the ER stress generation, apoptosis, and in vitro development of parthenogenetic porcine embryos. Porcine in vitro matured oocytes were activated by 1) electric stimulus (E) or 2) E+10 μM Ca-ionophore (A23187) treatment (EC). Oocytes were then treated by ER stress inhibitors such as salubrinal (200 nM) and tauroursodeoxychloic acid (TUDCA, 100 μM) for 3 h prior to in vitro culture. Parthenogenetic embryos were sampled to analyze ER stress and apoptosis at the 1-cell and blastocyst stages. The x-box binding protein 1 (Xbp1) mRNA and ER stress-associated genes were analyzed by RT-PCR or RT-qPCR. Apoptotic gene expression was analyzed by RT-PCR. At the 1-cell stage, although no difference was observed in Xbp1 splicing among treatments, BiP transcription level in the E group was significantly reduced by salubrinal treatment, and GRP94 and ATF4 transcription levels in EC group were significantly reduced by all treatments (p<0.05) compared to control. In the EC group, both apoptotic genes were reduced by ER stress inhibitor treatments compared to control (p<0.05) except Caspase-3 gene by TUDCA treatment. These results suggest that the treatment of ER stress inhibitor during parthenogenetic activation can reduce ER stress, and thereby reduce apoptosis and promote in vitro development of porcine parthenogenetic embryos.

본 연구의 목적은 1) 사회적 평가, 경쟁, 금전적 보상을 통한 스트레스 유발 처치가 시공간과제 수행에 미치는 영향과 2) 스트레스와 수행간의 관계를 매개하는 신경효율성의 변화를 조사하는 것이다. 본 연구의 피험자는 평균나이 23.4세의 대학생 15명으로 구성되었다. 피험자들은 스트레스 유발 처치조건과 무 처치조건에서 시각-모터 포인팅 과제를 수행하였고, 스트레스 유발 처치의 효과를 검증하기 위해 과제수행 시 각성측정의 지표인 심박수와 visual analogue scale(VAS)을 통해 경쟁심, 스트레스가 측정되었다. 또 스트레스 유발 처치가 수행력과 뇌 활성화에 미치는 효과를 검증하기 위해 과제수행 정확성과 뇌파(EEG)가 분석되었다. 먼저 스트레스 유발처치가 유의한 각성의 증가를 유발했는지를 검증하기 위해 스트레스 유발조건과 무 조건간 심박수, 경쟁심, 스트레스 척도에 대한 t-검증을 실시하였다. 또 과제수행시 뇌파의 차이를 비교하기 위해 조건(스트레스 유, 무), 뇌반구(좌, 우), 영역(전두, 중심, 두정, 측두, 후두엽)이 반복측정된 분산분석을 실시하였다. 이때 종속변인은 델타, 세타, 알파, 베타, 감마파의 파워값이었다. 분석결과, 스트레스 무 처치조건과 비교해서 유발조건에서 심박수, 경쟁심, 스트레스가 유의하게 증가하였다. 그러나 과제수행정확성은 무 처치조건보다 스트레스 조건에서 더 높은 것으로 나타났다. EEG 분석결과 스트레스 유발조건에서 시공간 정보처리를 담당하는 우뇌의 활성화가 스트레스 무 처치조건보다 높게 나타났고, 특히 이러한 조건별 차이는 과제 관련 뇌 영역인 측두엽에서 나타났다. 이 결과는 스트레스 유발 처치로 인한 과제 수행력 향상의 기전이 과제관련 뇌 영역의 활성화를 유도하는 신경효율성의 증가일 수 있다는 가능성을 제시한다.