Antioxidant activities and α-glucosidase inhibitory activities of Taraxacum officinale solvent fractions were measured. Extraction yields (relative to raw material) of 50% ethanol, hexane, ethyl acetate, butanol, and water were found to be 10.29, 2.61, 5.54, 2.15, and 0.96%, respectively. Polyphenol and flavonoid contents were high in ethyl acetate extract of Taraxacum officinale at 56.88 mg gallic acid/g and 33.27 mg gallic acid/g, respectively. DPPH, hydroxyl radical scavenging activity, and SOD-like activity measurement (IC50%) of Taraxacum officinale 50% ethanol extract, hexane, butanol, ethyl acetate, and water fractions were 22.64, 18.65, 10.29, 20.81, 20.46 mg/mL, 24.68, 10.69, respectively. It was found to be 9.66, 15.81, 13.77 mg/mL, 32.84, 17.09, 12.73, 33.63, and 33.91 mg/mL, and was high in the ethyl acetate layer. Results showed that α-glucosidase inhibitory activities of Taraxacum officinale solvent fraction were 25.75, 15.93, 35.87, 15.96, and 2.88% for 50% ethanol extract, hexane, butanol, ethyl acetate, and water fractions, respectively.

This study compared the nutritional composition and antioxidative activities of different parts (aerial part and root) of Taraxacum coreanum and Taraxacum officinale. To identify the nutrient composition of different parts of Daedelion species, nutrient contents were analyzed. Crude ash, protein, and fat contents of T. coreanum and T. officinale were higher in the aerial part compared to the root. There was no significant difference in crude ash, protein, or carbohydrate content of the aerial part depending on the Daedelion species. Aerial part contents of vitamin A, E, and C were significantly higher in T. coreanum than T. officinale. The K content of the aerial part of T. coreanum and T. officinale was highest, although there was no significant difference among Daedelion species. Contents of oxalic acid, citric acid, and acetic acid in the aerial part of T. coreanum were highest. Contents of total polyphenols, total flavonoids, and DPPH radical scavenging activity of the aerial part were significantly higher in T. coreanum than T. officinale. In conclusion, the aerial part of T. coreanum and T. officinale could be used as antioxidative functional food sources.

This study investigated the antioxidant effect of Taraxacumofficinale on the qualitycharacteristics of Yugwa during storage. Total polyphenol content was higher than total flavonoid content. DPPH radical scavenging activity and antioxidative index of Taraxacum officinale ethanol extracts were significantly (p<0.05) lower compared with those of Vit.C, whereas they were similar with those of BHA. The L, a, and b levels of Yugwa significantly (p<0.05) decreased with increasing amount of Taraxacum officinale powder; 6% Taraxacum officinale powder showed the lowest L and b levels. Hardness was unaffected by Taraxacum officinale content during the storage period. Brittleness of Yugwa was significantly (p<0.05) lower in control, 1.5%, and 6% Taraxacum officinale powder, whereas 3% Taraxacum officinale powder showed no significant differences during storage. Peroxide value significantly decreased in 3% and 6% Taraxacum officinale powders compared to those of control and 1.5% Taraxacum officinale powder after 4 weeks of storage. Taste, flavor, and crispness showed significant (p<0.05) differences according to Taraxacum officinale concentration. In conclusion, addition of 3~6% Taraxacum officinale powder provides beneficial effects in terms of storage life of Yugwa without any detrimental effects on quality characteristics.

최근 자연생태계에서 일어나고 있는 자생 민들레류의 감소와 서양민들레의 급속한 증가원을 종자 발아특성을 통해 알아보고자 한다. 각각의 종자는 무처리를 비롯하여 10일, 20일, 30일, 60일간의 저온처리를 실시하였으며 발아조건은 광조건과 암조건으로 실험하였다. 자생민들레의 경우에 광조건하의 무처리 대조구에서 28%의 발아율을 나타내었으나 60일간의 저온처리구에서 90%의 발아율을 나타내었다. 발아세와 평균발아기간도 30일 이상의 저온처리와 광조건하에서 양호한 성적을 나타내었다. 좀민들레의 경우에도 민들레와 거의 유사한 경향을 나타내어 30일 이상의 저온저리와 광조건하의 발아상태가 대조구에 비해 상대적으로 양호하였다. 그러나 서양민들레는 저온무처리구의 광조건에서 발아율 88.8%로 자생 민들레류에 비해 현저히 높게 나타났다. 또한 서양민들레 종자는 저온처리에 의핸 현저한 발아율 향상을 볼 수 없었다. 그러므로 30일 이상의 저온처리에 의해 휴면이 타파되고 발아율이 향상되는 자생 민들레류의 비휴면성인 성양민들에 비해 종 번식에 있어 상대적으로 불리하다는 것을 알 수 있었다.

서양민들레의 시설생산을 위해 배지조성 및 차광정도를 달리한 환경조건에 대한 생육특성과 그에 따른 생리활성 변이를 검토하고자 온실과 실험실 시험을 수행하였다. 서양민들레 시설생산을 위한 적정한 배지조합은 coco peat와 perlite를 각각 70 : 30과 50 : 50으로 혼합한 배지가 coco peat와 perlite 각각의 단독배지보다 생육 및 수량에 있어서 유의적으로 높게 나타났다(p<0.05). 차광정도에 따른 서양민들레의 초장, 근장, 엽수, 근직경, 엽면적, 엽록소 함량 및 생체중은 무차광이 50%와 70%차광보다 유의적으로 높게 나타났다(p<0.05). 총 페놀 함량과 총 플라보노이드 함량은 지하부보다는 지상부에서 높게 나타났다. 총 페놀 함량은 무차광에서 차광보다 높게 검출되었고, 총 플라보노이드 함량은 50%차광에서 가장 높았고 그 다음이 무차광과 70%차광 순으로 높았다. 한편, DPPH 라디컬 소거능과 아질산염 측정을 통한 항산화성은 추출물 농도가 증가할수록 높은 활성을 보였고 지하부보다는 지상부에서, 차광보다는 무차광에서 높은 활성을 보였다.

Dandelion has been widely utilized for medicinal and edible purposes. This research was conducted to evaluate the effect of supplemental LED (light-emitting diode) light on germination, growth characteristics and anthocyanin content of dandelion (Taraxacum officinale) seedling using LED blue (460 nm), red (660 nm, R), blue + red (B:R=6:4) and fluorescent lamp light treatment. By LED illumination to T. officinale seed germination speed was delayed, and germination rate was the highest in the fluorescent light. The growth characteristics (plant height, number of leaves, root length and fresh weight) were greatly influenced by supplemental LED light compared with control treatment, and the growth promotion was the most effective in the red LED illumination. After 60 days of red and mixed LED light treatments, anthocyanin content of dandelion plants was significantly changed. The anthocyanin content was increased by 12~19 mg/100 g under the red LED and the mixed light conditions compared with the control and the blue LED. Results indicate that illumination with red and mix LEDs, compared with other light treatments, is beneficial for promotion of growth and anthocyanin content in dandelion.

Ionizing radiations have been effective mutagen to overcome the limitation of the useful genetic resources in natural environment. This study was conducted to investigate an effect of proton beam on germination, growth patterns in the irradiated dandelion (Taraxacum officinale), and genetic variation in 12 morphological mutants induced in proton-beam irradiated dandelion. Percentage germination rate was drastically decreased over 250Gy. The lethal dose 50 (LD50) of germination was estimated between 250 Gy to 500 Gy. Significant decreases in growth patterns (plant height, number of leaf and fresh weight) were observed by increase of dose (Gy) of proton beam irradiation. According to the correlation analysis between dosage and growth factors, the orders of compactness of correlation were germination, plant height, fresh weight and number of leaf, respectively. Twelve morphological variants such as, dwarf, color, plastid, growth and leaf shape were screened at 50 to 250 Gy of the beam irradiation. As a result of ISSR analysis of the 12 variants, out of 33 bands detected overall, 8 bands were identified to be polymorphic with a rate of 24.2% at the control group. While 33 bands detected overall, 21 bands were identified to be polymorphic with a rate of 63.6% at the proton beam irradiation. The result indicates that the dandelion with proton beam treatment might be promoted variation at DNA level

Dandelion has been widely used as a folkloric medicine for treatment of diverse diseases. This study was conducted to evaluate the effect of light spectrum using red (660 nm), blue (460 nm), red and blue mixed (Red : Blue = 6 : 4) LED (light emitting diodes) and fluorescent lamp on growth and functional components of dandelion (Taraxacum officinale). When LED was illuminated to T. officinale cv. Goldenboll, seed germination was delayed, and germination rate was the highest in the control (fluorescent light). The growth (plant height, root length and fresh weight) except leaf number was increased under the LED treatments compared with the control, and the growth promotion was the most effective in the red LED illumination. Total polyphenol contents in dandelion irradiated with the red and blue mixed or the red LED were 121.77 mg% or 115.36 mg%, respectively, which were greater than those in dandelion treated with blue LED and fluorescent lamp. Asparagine, proline, serine, threonine, glutamic acid and arginine were the predominant amino acids in dandelion and total amino acid was the highest under the Red LED illumination. The results indicate that application of the red and the mixed LED illumination promote growth and increase functional components during cultivation of dandelion

This study was conducted to evaluate the effect of light spectrum using red (660 nm), blue (460 nm), red and blue mixed (Red : Blue = 6 : 4) LED (light-emitting diode), and fluorescent lamp on antioxidant activities and functional components of dandelion (Taraxacum officinale). Total polyphenol contents in dandelion irradiated with the red and blue mixed or the red LED were 121.77 mg/100 g or 115.36 mg/100 g, respectively, which were greater than those in dandelion treated with blue LED and fluorescent lamp. Asparagine showed the highest content among amino acids in leaves and roots regardless of treatments. Total amino acid was the highest when illuminated with the red LED. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity and SOD (super oxide dismutase) activity of ethanol extracts were increased under all of the LED treatments compared with fluorescent lamp, and the antioxidant activities were increased by the red and the mixed LED illumination. The results indicate that application of the red and the mixed LED illumination promote antioxidant activity and increase functional components during cultivation of dandelion.

서양민들레의 부위별 성분 및 생리활성 차이를 검토하고자 폴리페놀 함량, 플라보노이드 함량, 항산화성 및 세포독성을 분석하였다. Folin-Denis방법에 따른 총 페놀 함량은 꽃 추출물에서 72.0 mg kg-1으로 가장 높게 나타났으며, 그 다음이 잎, 뿌리, 꽃대 추출물 순으로 나타났다(p〈0.05). 한편, 총 플라보노이드 함량은 총 페놀 함량과 같은 경향을 보였으나 더 낮은 범위의 함량이 검출되었다. DPPH 라디컬 소거능은 추출물 농도가 증가할수록 높은 활성을 보였으며 그 중 꽃 추출물 (IC50 값 = 624.3 mg kg-1)에서 가장 높은 활성을 보였고 그 다음으로 잎, 뿌리, 꽃대 추출물 순으로 나타나(p 〈0.05), 이는 페놀 및 플라보노이드 함량이 DPPH 라디컬 소거능과 관련이 있음을 보여 주었다. MTT법에 의한 세포독성 시험에서 폐암세포주(Calu-6)와 위암세포주(SNU-601)의 세포 생존율은 꽃 추출물에서 가장 낮았고(IC50값 = 85.7과 311.4 mg kg-1), 그 다음으로 잎, 꽃대, 뿌리 추출물 순으로 뿌리 추출물이 가장 높은 생존율을 보였다. 이는 세포독성이 꽃 추출출물에서 가장 높고, 뿌리에서 가장 낮은 것을 보여 준 것이다. 결론적으로 총 페놀 함량과 총 플라보노이드 함량이 항산화성(r2=0.7280 to 0.9971)과 세포독성(r2=0.5795 to 0.9515)에 높은 상관관계가 있고, 그 함량과 활성은 식물체 부위별로 다르게 나타남을 확인하였다.

This study was carried out to investigate the physiochemical and microbiological changes of dandelion during fermentation. Thirty and fifty percentage raw sugar groups (SFE30 and SFE50) were introduced into dandelions and fermented for 120 days at 15-20℃. This study was conducted to investigate the utilization of sucrose on lactic acid bacteria from dandelions and their effect on the pH, titratable acidity, microorganism and formation of organic acids in dandelions during fermentation. The number of lactic acid bacteria increased remarkably up to 15 days of fermentation and then decreased rapidly thereafter. The maximum number of lactic acid bacteria, 7.9 log CFU/mL was reached at pH 4.17 and the pH of dandelion showed a slight decrease during fermentation and decreased steadily up to 90 days to reach an optimum pH of 4.0. The titratable acidity of dandelions fermented increased during fermentation. The concentration of organic acid, amino acid and free sugar in SFE30 was higher than both SFE50 and DWE. The results of dandelions fermented were remarkably retarded in the 50% raw sugar group compared to the 30% raw sugar group.

The study was conducted to investigate functional materials as skin whitening and anti-inflammatory agent from Taraxacum officinale and Taraxacum coreanum. The total polyphenol and flavonoid content in the ethanol extract of Taraxacum officinale were found to be 64.07mg/g and 32.46mg/g, respectively. In tyrosinase inhibitory activity, the hot water extract of Taraxacum coreanum was higher than the other extracts. However, in nitric oxide (NO) scavenging ability, the ethanol extract of Taraxacum coreanum was higher than the other extracts. the ethanol extract of Taraxacum coreanum showed strong NO production inhibitory effect in lipopolysaccharide (LPS)-stimulated Raw 264.7 cell. In the cell viability measurement by MTT assay and the lactate dehydrogenase (LDH) assay against L929 cell, the extracts were exhibited fine cell viabilities and normal LDH release levels as nontoxic result in sample concentration of 250~1000μg/ml. As a result, the ethanol extract and the hot water extract of Taraxacum coreanum could be applicable to functional materials for anti-inflammatory and skin whitening related fields, respectively.

The genetic diversity and the genetic relationship among 30 genetic resources of T. officinale and T. coreanum collected from 20 regions in Korea were evaluated by using ISSR markers. Out of 127 loci detected overall, 122 were identified to be polymorphic with a rate of 96.0% at the 30 individuals. The intraspecific polymorphism between T. officinale and T. coreanum was 92.6% and 88.2%, respectively. The genetic similarity matrix (GSM) revealed a wide range of variablility among the 30 accessions, spanning from 0.179 to 922. According to the clustering analysis, different species T. officinale and T. coreanum, were divided into independent groups and all of the accessions could be classified into 7 categories. Especially, all of the mountain collected accessions belonged to independent groups. The study findings indicate that T. officinale and T. coreanum accessions have a high genetic diversity and accordingly carry a germ-plasm qualifying as good genetic resources for breeding.

The propose of this study was to investigate the antioxidant activity of 80% ethanol extracts and various solvent fractions of dandelion (Taraxacmn officinale) leaves and roots, Total phenolics and phenolic acid contents were also examined. The total phenol content of leaves and roots were and respectively. Eight phenolic acids were separated by GC, among which caffeic acid (113.7 mg%)and m-coumaric acid (152.6 mg) were the dominant phenolic acids in leaves and roots, respectively. Amongst solvent functions of leaves and roots, the ethyl acetate fraction showed the strongest radical scavenging activity. A strong correlation was found between total phenol content and electron-donating ability, and ABTS radical scavenging activity showed a similar trend as electron-donating ability. Hydroxyl-radical-scavenging activity and lipid peroxidation were significantly higher in the ethyl acetate fraction than other factions. In particular, the SOD-like activity was highest (43.6%) in the ethyl acetate fraction of dandelion leaves, and was higher than that of trolox. Thus, the ethyl acetate fraction of dandelion leaves exhibited significant phenol content, antioxidant activity, and free-radical-scavenging effects.

Some studies of dandelion have been carried out on bioactivities, however, no comparative analysis on antioxidant and cytotoxic activities in the Korean dandelion (T. coreanum NAKAI.; KD) and dandelion (T. officinale WEB.) have been reported. In this study, the extracts of KD and dandelion analyzed relative phenolic contents and free radical scavenging, antioxidant enzyme and cytotoxic activities. The extract of Korean dandelion leaves (KDL) exhibited a higher phenolics content (368±11.5 mg/l00 g) and a strong free radical scavenging activity (RC50value;87.89 ug/ml) than other parts and BHT (120.12 ug/ml), synthetic antioxidant. At ascorbate peroxidase (APX) activity, the dandelion root (DR) had a greater (38.8 U/mg protein) antioxidant enzyme compare to the dandelion leaves (12.2 U/mg protein). The catalase (CAT) and superoxide dismutase (SOD) followed higher enzyme activity in Korean dandelion root (KDR) than other parts. In a cytotoxic activity against human cancer cell, the extracts of KDR was found to be active against Calu-6, HCT-116, and SNU-601 cell lines, with IC50 values of 522.34, 532.74 and 614.85 ug/ml, respectively. These results suggest that KD and dandelion would be an alternative antioxidant source, based on natural plant resources.