This study aimed to investigate the changes in bioactive compounds across the ripening stages of three pepper cultivars, each characterized by unique skin colors. The samples used in this study consisted of three pepper cultivars distinguished by their skin colors as green, purple, and yellow green at breaker ripening stage. Samples were harvested at each of the four ripening stages, including premature, breaker, turning, and mature, and subjected to analysis for various bioactive compounds, including capsaicin, ascorbic acid, kaempferol, quercetin, and sugars. In all cultivars with varying skin colors, the capsaicin content within green pepper fruits consistently increased as the ripening stages advanced. Ascorbic acid was most abundant during the premature stage of development in purple and green cultivars, subsequently declining as maturation progressed. In the case of the purple cultivar, kaempferol content decreased by approximately 30% at the mature stage, while the green cultivar exhibited a gradual increase in kaempferol content with maturation. Conversely, the kaempferol content of the yellow green cultivar rapidly declined as maturation progressed. Regarding quercetin content, the purple and green cultivars tended to decrease with maturity, while the yellow green cultivar displayed an increasing trend. Furthermore, the accumulation patterns of glucose, fructose, and sucrose, the predominant free sugars in green pepper fruit, demonstrated an inclination to increase as the maturation stage advanced in both purple and green cultivars. In contrast, the yellow green cultivar initially showed an elevation in free sugar content during the immature stage, followed by a minor reduction during maturation and a subsequent rise during the mature stage. Each pepper cultivar, distinguished by its unique skin color, exhibits varying levels of bioactive substances at different ripening stages. Therefore, optimal harvesting and utilization should align with periods when the desired substance content is at its peak.

Novel ionic liquid-functionalized carbon quantum dots (IL-CDs) were prepared by hydrothermal method, and characterized with FT-IR, TEM and XPS. The IL-CDs exhibited narrower particle size distribution with more uniform dispersion and the surface potential changes from negative to positive due to the function of IL. IL-CDs could be quenched (“turned off”) after adding ascorbic acid (AA), and as an “on–off”, fluorescent probe could be established for direct analysis AA. The linear range of AA was 0.34–30.00 μg/mL and the LOD was 0.11 μg/mL. The method was successfully applied to the determination of AA in real samples with satisfactory results.

본 연구는 ‘스마트꿀’ 참외를 수확 후 ascorbic acid (AA) 및 salicylic acid (SA) 침지 처리와 PE film 처리가 저온저장(10℃) 중 과골 갈변 발생과 과실품질 변화에 미치는 영향을 조사하였다. 참외 과실의 저장기간 동안 과골 갈변 발생 정도는 무처리 과실의 경우 저장기간 동안 꾸준히 증가하여 저장 15일후 3.8 수준으로 증가하였으나, PE film 처리 과실은 저장 15일후 2.7 수준으로 낮은 과골 갈변 증상을 보였다. 참외 과실의 고유색을 나타내는 황색 과피의 황색도(b*)의 경우, 무처리 과실은 저장 15일 후 38.1로 감소하였으나, PE film 처리 과실은 저장 15일 후에도 39.8로 높은 황색도를 보였다. 흰색 과골 명도(L*)는 무처리 과실은 저장 15일 후 67.9로 감소하였으나, PE film 처리 과실은 72.9로 높게 유지되었다. 그리고 흰색 과골 적색도(a*)의 변화는 무처리 과실의 경우 저장 15일 후 1.0으로 증가하였나, PE film 처리 과실은 저장 15일 후에도 –1.4 이었으며, AA 처리 과실 역시 –0.5로 적색도 변화를 효율적으로 억제하였다. 가용성 고형물 함량은 무처리 과실은 저장기간 동안 점차 감소하였으나 PE film, AA 및 SA 처리 과실들은 거의 변화가 없었다. 그리고 과실 중량감소율은 무처리 과실은 저장 15일 후 1.98%이었으나, PE film 처리 과실은 1.33%로 가장 낮은 중량감소율을 나타내었다. 따라서 ‘스마트꿀’ 참외를 저장 및 유통할 때 과골 갈변 발생과 과색 및 품질의 변화를 억제하는데 PE film 처리가 효과를 보여 처리방법에 대한 효율적인 적용 방안을 모색할 필요가 있다고 판단되었다.

L-ascorbic acid (L-AA; vitamin C) induces apoptosis in cancer cells. This study aimed to elucidate the molecular mechanisms of L-AA-induced apoptosis in human laryngeal epidermoid carcinoma Hep-2 cells. L-AA suppressed the viability of Hep-2 cells and induced apoptosis, as shown by the cleavage and condensation of nuclear chromatin and increased number of Annexin V-positive cells. L-AA decreased Bcl-2 protein expression but upregulated Bax protein levels. In addition, cytochrome c release from the mitochondria into the cytosol and activation of caspase-9, -8, and -3 were enhanced by L-AA treatment. Furthermore, apoptosis-inducing factor (AIF) and endonuclease G (EndoG) were translocated into the nucleus during apoptosis of L-AA-treated Hep-2 cells. L-AA effectively inhibited the constitutive nuclear factor-κB (NF-κB) activation and attenuated the nuclear expression of the p65 subunit of NF-κB. Interestingly, L-AA treatment of Hep-2 cells markedly activated Akt and mitogen-activated protein kinase (MAPK; extracellular signal-regulated kinase 1/2, p38, and c-Jun N-terminal kinase [JNK]) and and LY294002 (Akt inhibitor), SB203580 (p38 inhibitor) or SP600125 (a JNK inhibitor) decreased the levels of Annexin V-positive cells. These results suggested that L-AA induces the apoptosis of Hep-2 cells via the nuclear translocation of AIF and EndoG by modulating the Bcl- 2 family and MAPK/Akt signaling pathways.

Colon cancer is known as the third most widespread cancer in the world. The interaction of heme-iron and ascorbic acid (AA) in colon carcinogenesis is not evident. Hemin (ferric chloride heme) is an iron-containing porphyrin with chlorine that can be formed from a heme group. The purpose of this study was to investigate the protective effect of AA on the formation of pre-neoplastic lesions induced by azoxymethane (AOM)/dextran sodium sulfate (DSS) plus hemin in mice. Forty-five ICR male mice were divided into three experimental groups; AOM/ DSS treatment (control group), hemin (2 g hemin/kg of b.w.), hemin + AA (1.0% in drinking water). The mice had three s.c. injections (0–2nd weeks of the experiment) of AOM (10 mg/kg b.w.) weekly and 2% DSS as drinking water for the next one week and the animals fed on AIN-76A purified rodent diet for 6 weeks. The numbers of aberrant crypt foci (ACF) and aberrant crypts (ACs) in colonic mucosa were counted after methylene blue staining. Lipid peroxidation in feces was measured by the thiobarbituric acid-reactive substances (TBARS) assay. The numbers of ACF and ACs per colon significantly increased in Hemin group compared to the control group. However, the numbers of ACF and ACs per colon notably decreased in hemin + AA group compared to the control group or hemin group (p<0.05). In feces, the TBARS value of hemin group was higher than the control group (p<0.01). The TBARS value of hemin + AA group was slightly decreased compared to Hemin group. These results indicate that hemin can promote the experimental colon carcinogenesis in ICR mice. On the other hand, additional supplement of AA via drinking water has a protective effect against the colon carcinogenesis. The related mechanisms need to be illustrated by further studies in future.

The study aimed to determine effects of light emitting diode (LED) and the ultraviolet radiation (UVA) light of plant factory on plant growth and ascorbic acid content of spinach (Spinacia oleracea cv. Shusiro). Plants were grown in a NFT (Nutrient Film Technique) system for 28 days after transplanting with fluorescent light (FL, control), LEDs and UVA (Blue+UVA (BUV), Red and Blue (R:B(2:1)) + UVA (RBUV), Red+UVA (RUV), White LED (W), Red and Blue (R:B(2:1)), Blue (B), Red (R)) under the same light intensity (130 μmol·m-2·s-1) and photoperiod (16/8h = day/night). All the light sources containing the R (R, RB, RUV, and RBUV) showed leaf epinasty symptom at 21 days after transplanting (DAT). Under the RUV treatment, the lengths of leaf and leaf petiole were significantly reduced and the leaf width was increased, lowering the leaf shape index, compared to the R treatment. Under the BUV, however, the lengths of leaf and leaf petiole were increased significantly, and the leaf number was increased compared to B. Under the RBUV treatment, the leaf length was significantly shorter than other treatments, while no significant difference between the RBUV and RB for the fresh and dry weights and leaf area. Dry weights at 28 days after transplanting were significantly higher in the R, RUV and BUV treatments than those in the W and FL. The leaf area was significantly higher under the BUV treatment. The ascorbic acid content of the 28 day-old spinach under the B was significantly higher, followed by the BUV, and significantly lower in FL and R. All the integrated data suggest that the BUV light seems to be the most suitable for growth and quality of hydroponically grown spinach in a plant factory.

Colorectal cancer (CRC) is the third most prevalent cancer in the world, and heme iron is known to promote the CRC in an animal model. This study was conducted to investigate the effects of ascorbic acid in the presence of hemin on the formation of pre-neoplastic lesions induced by azoxymethane (AOM)/disodium sulfate (DSS) in mice. After acclimation for 1 week, five-week old mice received three s.c. injections (0-2 weeks of the experiment) of AOM [10 mg/kg body weight (BW)] weekly and were treated with 2% DSS in drinking water for the next week to induce aberrant crypt foci (ACF). All animals were fed the AIN-76A purified rodent diet for experimental period of 6 weeks. Experimental groups were then divided into three groups: carboxymethylcellulose (CMC) alone (control), CMC + Hemin, CMC + Hemin + ascorbic acid (AA). The CMC was used as a solvent for hemin. The daily doses were 534 mg/kg BW hemin and 246 mg/kg BW ascorbic acid administered orally. After the colonic mucosa were stained with methylene blue, aberrant crypt foci (ACF), aberrant crypt (AC) and polyps were counted. Lipid peroxidation in liver was evaluated by the thiobarbituric acid-reactive substances (TBARS) assay. The numbers of ACF, AC and large ACF (≥4 AC/ACF) per colon increased in the hemin group compared to the control group, while they decreased significantly in the hemin + ascorbic acid group compared to the control group or hemin group (p<0.01). The number of polyps/colon in the hemin + AA group was significantly decreased compared to the hemin group (p<0.05). In the liver, the TBARS value of the hemin group was significantly higher than that of the control group (p<0.01). Additionally, the TBARS value of the hemin + AA group decreased slightly compared to that of the hemin group. Taken together, these results suggest that hemin can promote colon carcinogenesis in a mouse model and that ascorbic acid has a protective effect against hemin-promoted colon carcinogenesis.

Excessive iron can promote the production of free radicals, thereby leading to harmful effects on cancer and aging. Ascorbic acid is not only an antioxidant but also a co-factor of iron absorption. The effect of iron-overload with ascorbic acid on experimental colon carcinogenesis was investigated in male ICR mice. Animals were treated weekly with azoxymethane (AOM, 10 mg/kg b.w.) at 0, 1, and 2 week and then drunk 2% dextran sodium sulfate (DSS)-containing water for the next 1 week. There were four experimental groups: carboxymethylcellulose (CMC) alone (control), CMC + ascorbic acid (AA), CMC + Fe, CMC + Fe + AA. The animals fed on AIN-76A purified rodent diet for six weeks. AA or Fe2O3 at the dose of 450 mg/kg b.w. were daily and orally treated for 6 weeks. The colonic mucosa was stained with methylene blue and then aberrant crypt foci (ACF) and polyps were counted. Thiobarbituric acid-reactive substances (TBARS) in serum and liver were determined. Iron concentration in liver was measured by inductively coupled plasma spectrophotometer. Fe-overload with AA strongly increased liver iron contents compared to control or Fe group (p<0.05). There were no significant differences in the number of ACF or polyps among all groups, although ironoverloaded groups had slightly higher numbers compared with the control or AA group. TBARS values in the liver were increased in the iron-overloaded groups compared to control and AA only group (p<0.05), but serum TBARS values were not changed. These results indicate that the excessive iron treatment did not affect the experimental colon carcinogenesis regardless of presence of AA in mice.

Iron-overload can cause harmful effects such as cancer and aging via promoting the production of free radicals. The effect of orally administered nano-Fe overload with ascorbic acid on colon carcinogenesis was investigated in male ICR mice. After a 1-week acclimation, 5-week-old mice received three intraperitoneal injections (experimental week 0-2) of azoxymethane (AOM, 10 mg/kg body weight) weekly, followed by 2% dextran sodium sulfate (DSS) in drinking water for the next 1 week to induce aberrant crypt foci (ACF). Animals were divided into four groups; carboxymethylcellulose (CMC) alone (control), CMC + ascorbic acid (AA), CMC + nano-Fe (NFe), and CMC + NFe + AA groups. Animals were fed an AIN-76A purified rodent diet and daily administrated oral doses of 450 ppm each of nano-Fe and AA combination for 6 weeks. The colonic mucosa was stained with 0.5% methylene blue, and then the ACF and polyps were counted. Lipid peroxidation in the serum and liver was evaluated using the thiobarbituric acid-reactive substances (TBARS) assay. Iron concentration in the liver was measured using Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES). Iron concentration in the liver of the NFe-overloaded groups was higher than that of the control (p<0.05). AA treatment increased the iron concentration in the liver. The number of ACF was not significantly different among all the groups. The number of polyps in all the NFe-treated groups was slightly higher than that in the control group and AA only-treated group. The serum TBARS was not significantly different among all the groups, but that in the liver was higher in all the NFe-treated groups than it was in the control group (p<0.05). These results indicate that the additional NFe treatment did not affect the experimental colon carcinogenesis in mice regardless of the presence of ascorbic acid.

The objective of this study was to determine the effect of drinking water containing trimethyl glycine or ascorbic acid on growth performance and blood parameter profiles of duck exposed to scorching heat stress. A total of 480 ducks were randomly assigned to the following eight experiment groups for 42 days : control group C with general water, treatment group 1 (T1) with drinking water containing 100 ppm ascorbic acid, treatment group 2 (T2) with drinking water containing 200 ppm ascorbic acid, treatment group 3 (T3) with drinking water containing 300 ppm ascorbic acid, treatment group 4 (T4) with drinking water containing 400 ppm trimethyl glycine, treatment group 5 (T5) with drinking water containing 800 ppm trimethyl glycine, treatment group 6 (T6) with drinking water containing 1,200 ppm trimethyl glycine, treatment group 7 (T7) with electrolytes of KCl (0.5%) + NaHCO3 (1.0%)+NaCl (0.5%). Our results revealed that the body weights and feed intakes of treatment groups, especially T3 and T6, were increased compared to the control group, where as the feed conversion ratios of treatment groups were decreased (p<0.05). Blood levels of total cholesterol, triglyceride, LDL-C, glucose, AST, ALT and pH in treatment groups were lower compared to those in the control group (p<0.05). Blood levels of red blood cell, platelets profiles, electrolyte and gas in treatment groups were higher compared to those of the control group (p<0.05).

Mulberry fruits were semi-dried using hot air (60-100℃) or cool air (20-40℃), and the effects of the dryingtemperature and processing time on the quality of the final driedmulberry fruits were investigated. Response surface methodologywas employed to establish a statistical model and predict theconditions resulting in minimal loss of the total phenolic content(TPC) and ascorbic acid. Thus, using overlapped contour plots,the optimal conditions for producing semi-dried mulberry fruits,which reduced the moisture residue to 45% and minimized thenutrient losses of TPC and ascorbic acid, were determined for thehot-air process (60.7℃ for 5.4h) and cool-air process (34.8℃ for23.3h). Plus, a higher drying temperature was found to lead to afaster loss of moisture and ascorbic acid, while the TPC wassignificantly decreased in the cool-air dried mulberry fruits due tothe higher activity of polyphenol oxidase between 30 and 40℃.

본 연구는 절화 보존용액의 sucrose, 8-HQS, pH 그리고 유기산 등이 수국 ‘Magical Brilliant’, ‘MagicalJewel’의 절화수명에 미치는 영향을 알아보기 위하여수행하였다. 실험환경은 온도 24±2, 상대습도(relativehumidity, RH) 60~70%, 광도 11µmol•m−2•S−1 PAR로 하였고, 형광조명 16시간으로 하였다. 보존용액에함유된 sucrose와 8-HQS 의 효과에 있어서 ‘MagicalBrilliant’의 경우 절화수명은 3% sucrose+8-HQS 혼용처리에서 9.3일로 대조구 4.7일보다 4.6일 길었으며생체중과 용액흡수량도 가장 높게 나타났다. ‘MagicalJewel’에서의 절화수명은 1% sucrose+8-HQS 혼용처리에서 20.0일로 가장 높게 나타났으며 대조구에 비해 12.3일 연장되었고 용액흡수량과 생체중의 변화는 꾸준하게증가하는 경향을 보였다. 화색유지와 절화수명에 미치는sucrose와 8-HQS의 혼용처리의 효과는 단용처리에 비해 뚜렷하게 나타났다. 보존용액의 산도에 따른 효과는pH 3.5에서 가장 좋았다. 절화수명일에 있어서 ‘MagicalBrilliant’는 9.0일 그리고 ‘Magical Jewel’은 21.7일로 가장 높게 나타났다. 보존용액의 산도가 중성 쪽으로 갈수록 절화수명일, 생체중 및 용액흡수량 등이 감소하는 경향을 보여 보존용액의 산도가 절화품질에 영향을미치는 중요한 요인으로 생각된다. 유기산 처리의 결과,‘Magical Brilliant’과 ‘Magical Jewel’은 100mg•L−1citric acid 처리에서 각각 절화수명이 12.3일과 22.7일로다른 처리구보다 가장 높게 나타났다. 절화품질을 향상시키기 위해서는 ‘Magical Brilliant’과 ‘Magical Jewel’모두 3% sucrose+250mg•L−1 8-HQS(pH 3.5), 3%sucrose+250mg•L−1 8-HQS+100mg•L−1 citric acid를 처리하는 것이 가장 양호한 것으로 판단된다.

hydroxycinnamic acid인 ferulic acid와 caffeic acid는 강력한 식물성 항산화제 이다. DPPH 수용액에서 이들의 자유라디칼 소거능과 높은 온도, 금속 이온과 같은 산화촉진 요인에 대한 화학적 안정성 등을 살펴보았다. 아스코르빅 산의 안정성 향상을 위해 ferulic acid와 caffeic acid를 아스코르빅산 수용액에 첨가하였다. 아스코르빅 산의 안정성 향상은 시간경과에 따른 SC50 값의 변화로부터 확인하였다. 아스코르빅 산을 ferulic acid 또는 caffeic acid와 조합하여 BGsome 안에 포집시켜본 결과, 아스코르빅 산의 안정성이 순수한 용액 상태로 있을 때에 비해 크게 향상되었다.

Iron nanoparticles (Fe-NPs) have recently been used for cancer diagnosis and therapy for imaging contrast and drug delivery. However, no study on nutritional bioavailability of Fe-NPs in the body has been reported. Ascorbic acid (AA) is known to aid in absorption of iron in the stomach by reducing Fe (III) to Fe (II). In this study, we investigated the bioavailability of Fe-NPs with AA in iron-deficiency-anemic mice in comparison with non-nano iron particles. Iron-deficient anemia was induced by feeding an iron-deficient diet (4.5 mg Fe/kg) and ocular bleeding from retro-orbital venous plexus for four weeks. Normal control mice were given a normal diet (45 mg Fe/ kg). After induction of anemia in mice, anemic mice received daily oral administration of Fe (40 mg/kg B.W.) + AA (5 g/kg B.W) and Fe-NPs (40 mg/kg B.W) + AA (5 g/kg B.W). After sacrifice, liver and spleen tissues were observed by haematoxylin & eosin stain. Amount of trace iron in liver and upper small intestine was investigated using an inductively coupled plasma-atomic emission spectrometer. Red blood cells (RBC), hematocrit (Hct), hemoglobin (Hb), and total iron binding capacity were also measured. The concentrations of iron in the Fe-NPs + AA group were significantly higher in liver and in upper small intestine than that in the Fe + AA group. The values of RBC, Hct, and Hb in the Fe-NPs + AA group were more rapidly increased to normal values compared with the Fe + AA group with increasing time. These results suggest that Fe-NPs in the presence of AA may be more bioavailable than non-nano Fe in Fe-deficient anemic mice.

This paper examines the effects of various methods of soft steaming(i.e., forced convection-boiler, forced convection-fan, and natural convection) on the quality of potatoes. In particular, the paper investigates the effects of cooking conditions (the steaming method, the treatment time, and the temperature) on the color(L, a, b), moisture content, texture profile, and ascorbic acid of potatoes. The results indicate that not only the cooking method, the treatment time, and the temperature but also the heat transfer mechanism had considerable influence on potato quality. In addition, natural convection steaming was superior to other treatment methods in terms of nutrient retention and texture maintenance. The results of this study should be useful for establishing commercial standards for processing potatoes and improving the quality of thermally processed foods.

Encapsulation of L-ascorbic acid(AA) into BGsome was attempted to improve its stability. BGsome is a bio-compatible vesicular system prepared by dispersion of hydrated liquid crystalline phase formed through hydration of 1,3-butylene glycol(BG)-dissolved lecithin with an aqueous solution containing hydrophilic component. The characteristics of AA encapsulated BGsome, such as droplet size, surface charge, and solution appearance, was investigated. The concentration of AA solution had considerable effect on droplet size and surface charge of BGsome. Several tens nanometer droplet made by sonication treatment did not showed any change of size with storage time. Stability of AA was improved by encapsulation into BGsome, which was verified through DPPH test and HPLC assay.

산화방지제인 아스코르빈산(AA), 에리쏘르빈산(isoAA), 아스코르빌파르미테이트(AP), 아스코르빌스테아레이트(AS)에 대한 ‘식품 중 식품첨가물 분석법’을 검증하고 미비점을 개선하였다. 고속액체크로마토그래피 자외선검출기법을 이용하여 검출한계(LOD), 정량한계(LOQ), 상관계수(R2) 등을 측정하였고 돼지기름(lard), 사이다를 이용한 모델식품에서의 회수율과 재현성을 측정하였다. AA와 isoAA의 검출한계는 각각 0.46, 0.48 μg/mL이었으며, 회수율은 각각 86.35-94.78, 84.76-95.02%를 나타내었고 상관계수는 모두 0.999이상을 보였다. AP와 AS의 현 분석법은 메탄올을 용매로 사용하지만 메탄올 용매에서 AP와 AS는 불안정하였다. 냉장온도에서 에탄올을 용매로 사용 시 다른 용매에 비해 유의적으로 높은 안정성을 나타내어 기존 용매의 불안정성을 개선할 수 있었다.

고랭지에서 재배되는 주요 양채류의 기능성 향상을 위한 적정 셀레늄처리 방법을 구명하고자, sodium selenate처리 농도, 처리시기 및 처리 횟수에 따른 작물생육과 작물체내 무기성분, ascorbic acid, nitrate 및 셀레늄 함량에 미치는 영향을 조사하였다. Sodium selenate 1, 2, 5 및 20mg·L-1처리구에서 공시작물 모두 초기생육은 큰 차이가 없었으나, 처리 60일 후부터 5mg·L-1 이상의 고농도에서는 생육이 크게 억제되어, 무처리구에 비해 5mg·L-1처리구에서도 결구상추는 33%, 브로콜리는 47%, 파슬리는 74% 생체중이 감소한대 비해 비트와 셀러리는 고농도에서도 생육억제 현상이 크지 않아 20mg·L-1농도에서 생체중이 20%와 15% 감소하였다. sodium selenate 처리에 따른 작물체내 ascorbic acid 함량은 결구상추의 경우 셀레늄처리 농도가 높아질수록 증가하는 경향을 보여 20mg·L-1처리구는 무처리구에 비해 약 1.2배 높았고, 셀러리와 비트도 같은 농도에서 약 10% 정도 함량 증가를 보였으나, 브로콜리와 파슬리는 셀레늄처리에 따른 통계적 유의차는 없었다. 식물체내 nitrate함량은 무처리구에 비해 모든 작물에서 감소하였으며, 처리농도가 증가할수록 감소의 폭은 큰 경향을 나타내었다. 작물별 질산염의 함량 저하는 결구상추에서 가장 현저하였으며, 그 다음이 비트, 셀러리 순이었다. 무기성분 K, Ca, Mg의 함량은 공시작물 모두 처리농도가 높아질수록 감소하는 경향을 보였다. 성분별로는 K성분이 모든 작물에서 고도의 부(負) 상관관계를 나타냈으나, Mg와 Ca함량이 감소는 농도간의 차이에 유의성이 없었다. Sodium selenate 처리에 따른 식물체내 셀레늄함량은 처리 농도가 증가함에 따라 모든 공시작물에서 비례적으로 증가하여, 고농도인 20mg·L-1 처리구에서 브로콜리는 무처리구 보다 24.4배, 셀러리는 76.4배, 파슬리는 560배의 높은 함량을 보였으며, 결구상추, 비트도 같은 경향을 보였다. 작물의 생육단계별 처리에서는 생육초기에 후기보다 결구상추는 1.3배, 브로콜리는 1.4배 높았으나, 파슬리와 셀러리는 생육중기에 처리한 것이 가장 높은 함량을 보였다. 처리 횟수별 Se 함량은 파슬리, 셀러리 및 결구상추는 처리 횟수에 비례하여 증가하다가 10회 이상이 되면 셀레늄의 축적량이 둔감해지는 경향을 나타냈으나, 브로콜리는 처리횟수가 많으면 많아질수록 셀레늄의 축적량도 지속적으로 증가하는 경향을 나타냈다.