This study was designed to evaluate the antioxidant activities and protective effects on PC12 cells of the extract of Epimedium koreanum and its main constituents icariin and icariside I. After screening the seven identified flavonoid glycosides from E. koreanum through DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay, E. koreanum, Icariin and Icariside I exhibited significant effect on radical scavenging activity. E. koreanum, icariin and icariside I were examined using DPPH, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) and FRAP (ferric reducing ability power) assay. In all antioxidant assays, E. koreanum, icariin and icariside I showed high radical scavenging activities in a dose-dependent manner. Protective effects against H2O2-induced PC12 cells were assessed with MTT assay. The results indicated that cell viability and protection on PC12 cells of icariside I and icariin increased dose dependently. These study results suggest that E. koreanum, icariin and icariside showed high antioxidant capacities and cell protective effects. Icariside I, one of the metabolites of icariin, may be a new and effective flavonoid compound as a functional component.
The repellent activities of 33 plant extracts against Aedes albopictus were examined using a patch test for adult. The six plant extracts (Magnolia denudate, Dryopteris crassirhizoma, Cnidium officinale, Lindera obtusiloba, Magnolia kobus, and Houttuynia cordata) showed over 70% repellency activities to Ae. albopictus. The insecticidal activity of Curcuma longa against Ae. albopictus was also determined. C. longa hexane extraction showed 100% larvicidal activity at 1,000 ppm after treated 24 h. Purification of the biologically active constituents from the hexane extraction with larvicidal activity was done using silica gel column chromatography. H1 fraction gave 100% mortality to Ae. albopictus at 100 ppm. H12 fraction was determined 87.8% larvicidal activity to Ae. albopictus at 50 ppm. Active constituent was analysed as the sesquiterpene, ar-turmerone (C15H20O) by GC and GC-MS. C. longa extract gave highly protection against mosquito bites. The hexane extraction showed complete protection at 10 mg over 6 h. In this study, the sesquiterpene from C. longa induced a protection time, repellency, landing time, and biting time against Ae. albopictus.
These results suggest that the some plant extracts and C. longa have the potential to be used an eco-friendly materials for the control of mosquitoes.
Extracts from Aloe vera leaves, Aloe arborescens leaves, Aloe vera callus, Portulaca oleracea and cacao (Theobroma cacao L.) bean husk (CBH) were prepared using acetone, chloroform, ethanol, hexane, and water. Solvent extracts of Aloe vera leaf had very high antioxidant activities showing IC50 values in the ranges of 0.02-0.17 mg/ mL, and had the highest total phenolic and flavonoid content among the tested samples. We hypothesized that Aloe vera leaf and CBH extracts might possess considerable in vitro anti-glycation activities. Indeed, these extracts strongly inhibited the formation of advanced glycation end-products from RNase in the presence of ribose. The chloroform extract of Aloe vera leaf showed the strongest inhibition of AGE formation (99.9%), followed by the 95% acetone extract (92.8%) at a concentration of 1 mg/mL, exhibiting higher anti-glycation activities than those of AG and rutin (73.4% and 96.1% at 1 mg/mL, respectively). The anti-glycation activity of all extracts was correlated positively with their total contents of phenolics and flavonoids. We conclude that Aloe vera leaf extracts and their constituents may be used as anti-glycation agents.
국내에서 유통되고 있는 무기배지재료인 버미큘라이트(12점)와 펄라이트(5점)의 물리 화학적 특성을 분석하여 혼합배지 조제를 위한 기초자료를 얻기 위하여 본 연구를 수행하였다. 710μm 이상의 직경을 갖는 입자의 비율이 버미큘라이트 중 중국산 silver 3~8mm가 99.7%로 조사되어 가장 비율이 높았고, 펄라이트는 3~5mm에서 99.9%로 가장 높았다. 펄라이트(〈1mm)와 남아프리카공화국산 silver 버미큘라이트(0.25~0.7mm)의 용기용수량은 각각 72.0 및 71.1%로 가장 높았고, 기상률은 중국산 silver 3~8mm가 49.3%로 뚜렷하게 높았다. 펄라이트 5종류의 공극률은 60% 이상으로 측정되었으며, 용기용수량은 1mm 이하의 규격을 갖는 물질을 제외하고 20.4~39.7%의 범위에 포함되었다. 버미큘라이트 중 중국산 gold 0.3~1mm와 남아프리카공화국산 0.25~0.7mm가 쉽게 이용할 수 있는 수분(EAW)와 완충수(BW)의 비율이 높았고, 펄라이트는 1mm 이하와 0.7~1.5mm의 EAW와 BW의 비율이 높았다. 화학적 특성에서 버미큘라이트는 pH가 6.36~10.74 범위에 포함되는 강알칼리성 물질이었고, 전기전도도(EC)는 0.032~0.393dS·m-1로 측정되어 종류간 차이가 큼을 알 수 있었다. 펄라이트의 pH는 7.788.62 범위에 포함되어 약알칼리성이었으며, EC는 0.030~0.041dS·m-1로 측정되었다. 양이온교환용량은 중국산 silver 버미큘라이트 0.3~1mm가 14.7cmol+·kg-1으로 가장 높았고, 펄라이트는 0.71.5mm가 1.51cmol+·kg-1로 가장 높았는데 버미큘라이트보다 약 10배 가량 낮았다. 버미큘라이트는 펄라이트보다 Ca, K 및 Na과 같은 치환성 양이온 함량이 많았다.
Porous carbon materials synthesized from various plant derived precursors i.e. seeds of [Castor (Ricinus communis), Soap nut (Sapindus sp.), Cashew-nut (Semecarpus anacardium), Jack fruit (Artocarpus heterophyllus), Safflower (Carthamus tinctorius), Ambadi (Crotolaria juncea), Neem (Azadirachta indica), Bitter Almond (Prunus amygdalus), Sesamum (Sisamum indicum), Date-palm (Phoenix dactylifera),Canola (Brassica napus), Sunflower (Helianthus annulus)] and fibrous materials from [Corn stem- (Zea mays), Rice straw (Oryza sativa), Bamboo (Bombax bambusa) and Coconut fibers (Cocos nucifera)] were screened to make supercapacitor in 5M KOH solution. Carbon material obtained from Jack fruit seeds (92.0 F/g), Rice straw (83.0 F/g), Soap nut seeds (54.0 F/g), Castor seeds (44.34 F/g) and Bamboo (40.0 F/g) gave high capacitance value as compared to others. The magnitude of capacitance value was found to be inversely proportional to the scan rate of measurement. It is suggested that carbon material should possess large surface area and small pore size to get better value of capacitor. Moreover, the structure of carbon materials should be such that majority of pores are in the plane parallel to the plane of electrode and surface is fluffy like cotton ball.
본 연구는 압화의 분야 중 가장 큰 비중을 차지하 고 있는 풍경화 작품에 대해 조사하였다. 2000~2007 년 출품작 중에서 풍경화 80작품을 선택하여 풍경화작 품에 사용되는 식물소재 및 부재료 등을 조사하였다. 풍경화 작품에서 사용된 주요 식물소재의 종류를 조사 한 결과 초본식물 소재의 사용이 67%로 목본식물 소 재의 33%보다 사용되는 비중이 컸다. 많이 사용된 초 본식물 소재는 루모라고사리, 이끼, 아디안텀, 냉이 등 이었고, 목본식물 소재는 편백, 황금편백, 포도나무 등 이었다. 봄 풍경화작품에 많이 사용된 식물소재는 냉 이, 포도나무, 조팝나무 등이고, 여름 풍경화작품에는 편백, 세라기넬라, 아디안텀, 가을 풍경화작품은 기린 초, 편백, 단풍나무, 겨울 풍경화작품에는 포도나무, 백 묘국, 에버라스팅 순으로 많이 사용되고 있었다. 식물 소재의 염색 유무조사에서 거의 대부분이 무염색 식물 소재였고, 일부 염색되는 식물소재는 조팝나무, 냉이, 쉬땅나무, 까치수염 등 흰색의 꽃과 편백, 세라기넬라 등의 잎이었다. 식물소재의 변색 정도에서 사계절 모두 변색이 잘 안 되는 소재가 67% 이상 사용되고 있었다. 식물소재 이외에 사용되는 부재료의 경우 바탕지는 압화전용 종이 65%, 한지 21%, 아크릴판 9% 등의 순이었고, 바탕지 위에 첨가된 부재료는 아크릴물감, 한지, 락카, 솜 등의 순으로 많이 사용되는 것으로 조 사되었다.
발전소 내 방사화 부식생성물의 대부분을 차지하고 있는 니켈 페라이트계 부식생성물을 모사 발생시키기 위한 고온 고압용 장치를 제작하여 연구를 수행하였다. 배관형 포집기를 이용한 부식생성물 발생장치로부터 방사화 부식생성물과 가장 유사한 부식생성물을 얻을 수 있었다. 발전소에서 입자성 부식생성물이 발생되는 원리인 온도에 따른 용해도 차이를 구현하기 위하여 270C에서 부식반응이 일어나 상대적으로 높은 온도를 가진 포집용 장치에 부식생성물이 포집되도록 장치를 제작하였으며 , 발생된 부식생성물은 주사전자현미경 관찰과 EDAX를 통한 조성분석으로 그 특성을 관찰하였다. 부식생성물은 포집 된 위치 에 따라서 침상 형태의 산화물과 결정 형태의 산화물로 나뉘었으며, 조성 분석 결과 결정 형태의 부식생성물이 니켈 페라이트로서 발전소에서 발생되는 입자성 부식생성물과 유사한 것을 알 수 있었다.
The objectives of this study were to assess plant growth of the flowering shrub species for adapting to growing on hanging baskets in the vertical greenery system with different cover materials and substrates for low maintenance effectively utilizing rainfall. Water amount taken up by the fabric cover was higher than that by the plastic film cover. Evaporated water amount from the fabric cover was much slower than that from the plastic cover. A higher ratio of hydrophilic polymer to the substrate resulted in higher substrate water content within the same cover material. The relative growth rates of plant height, leaf width and stem diameter were higher when the Cotoneaster horizontal was grown in the vertical greenery system with the fabric cover, whereas the leaf number and survival rate were higher with the plastic film cover. The relative growth rates of plant height, leaf width and leaf number and the survival rate was higher when the Euonymus fortunei ‘Emerald n Gold’ was grown in the vertical greenery system with plastic film cover than those with both fabric cover and hydrophilic polymer treatment. In contrast, the stem diameter of Euonymus fortunei ‘Emerald n Gold’ was higher in the plants grown in the vertical greenery system with the fabric cover at a 1.0% ratio of hydrophilic polymer to substrate than that with plastic film cover. The highest stem diameter of Spiraea × bumalda ‘Gold Mound’ was observed in the plant grown in the vertical greenery system with fabric cover and at a 1.0% ratio of hydrophilic polymer to substrate, while there was no significant difference in the plant height. The leaf number was decreased with increase in the ratio of hydrophilic polymer to substrate, while the leaf width was decreased. The survival rate of Spiraea × bumalda ‘Gold Mound’ was higher than 80% regardless of treatments. In particular, the highest survival rate was observed in the plastic film cover treatment after overwintering.