Phalaenopsis ‘KS Little Gem’ is a new cultivar with superior ornamental qualities. Newly produced cultivars are often studied to determine the optimum growing conditions, as production management practices vary within species. We focused on the effects of temperature and fertilizer application on the growth and carbohydrate content of ‘KS Little Gem’ plantlets. Two-month-old plantlets were subjected to different temperatures (20, 25, and 30°C) and fertilizer rates (0.33, 0.5, and 1 g･L-1) inside a growth chamber. Data were collected from leaves and roots regarding their growth, fresh weight, sugar content, and starch content. The results revealed that temperature significantly affected leaves and roots parameters, fresh weight, and carbohydrate content of the plantlets. However, fertilizer rates alone showed no significant effects on the growth and carbohydrate content of plantlets. The highest leaf and root growth, root fresh weight, and leaf carbohydrate content were observed in plantlets subjected to 25°C. In addition, ‘KS Little Gem’ plants grown under 30°C had significantly inhibited growth and reduced carbohydrate content in both the leaves and roots.

고구마 바이러스 무병묘의 기내급속증식을 위한 적정 생장 조절물질 및 sucrose 농도, 최소생장 기내보존(15°C)에 미치는 광의 영향과 생존율 및 기내생장 특성 등을 조사하였다. 고구마 무병묘의 마디배양은 0.2mg·L-1 BA 첨가배지에서 줄기신장, 줄기직경, 잎수, 뿌리수, 생체중 및 건물중 등이 가장 양호하였다. 배양부위 및 배지물리성에 따른 적정 sucrose 농도는 마디배양은 5% sucrose를 첨가한 고체배지에서, 정단배양은 3% sucrose를 첨가한 액체배지에서 줄기두께, 잎수, 뿌리수, 뿌리길이, 생체중 및 건물중 등의 생육에 가장 효과적이었다. 15°C 저온항온기에서 고구마 무병묘의 최소생장 기내보존은 암상태에서는 3개월 내에 모두 고사하였으나, 적색:청색(7:3) 혼합 LED (150±5μmol·m-2·s-1 PPFD)에서는 5개월까지 100% 생존하였다. 따라서 고구마 무병묘의 최소생장 기내보존에는 광이 필요하며, 샬레에 밀식(10 개체/샬레)할 경우, 좁은 공간에서 대량보존이 가능하였다.

In this study, the principal objective was to investigate the effect of light quality and vessel ventilation on the growth and development, physiology, activities of antioxidant enzymes, and contents of mineral nutrients of carnation (Dianthus caryophyllus L.) ‘Marble Beauty’. Single node cuttings stuck into the plant growth regulator (PGR)-free MS medium in containers covered with caps with or without a ventilation filter were cultured first four weeks under white and then additional four weeks under either white (control), blue, red, or red + blue light emitting diodes (LEDs) for 56 days. Interestingly, a ventilated culture condition not only reduced the percentage of the hyperhydricity, but also increased the total chlorophyll content (Chl a + Chl b) of the plantlets as compared to the non-ventilated condition. In addition, blue LEDs produced plantlets with the greatest number of shoots and red LEDs produced plantlets with the greatest shoot length. The quality of plantlets was improved under a ventilation condition. Besides, under a ventilated condition, red + blue LEDs raised APX activity, and blue LEDs not only raised the activity of the CAT, but also increased tissue contents of such elements as K, Ca, Mg, Zn, Mn and Fe. The red LEDs increased contents of B and Si under a ventilated condition, and Na accumulation under a non-ventilated condition. Thus, including blue or red LEDs as the light source in a ventilated culture condition will produce plantlets of carnation ‘Marble Beauty’ in vitro with improved quality.

Sweet potato [Ipomoea batatas (L.) lam] plant is known to have the relatively tolerance soil moisture deficit compared to other upland crops. However, recently, it has been causing plant growth inhibition and decrease storage quality due to the drought during the growing season of sweetpotato in Korea. The response of root elongation, leaf and shoot growth of sweet potato plantlets, cultivated under different water potential conditions were observed. Six varieties, such as yellow fleshed (Jinhongmi), dark-yellow fleshed (Daeyumi), dark-orange fleshed (Juhwangmi), light-orange fleshed (Dahomi, Pungwonmi), and purple fleshed (Sinjami) were used in this experiment. Using the follow water osmotic potential (WOP) levels were 0 (control), -0.05, -0.1, -0.2, -0.3, -0.4, -0.5 MPa by polyethylene glycol (PEG). In in vitro culture condition, ‘Jinhongmi’ has been inhibited rooting number and number of leaves less than 2, although slightly low WOP of–0.05 and –0.1 MPa, and at –0.2 MPa. shoot was not growing. ‘Daeyumi’, Dahomi, and Juwhangmi’ produced 2 roots as a low WOP of –0.3 MPa. and at –0.2 MPa., it appeared 2 or 3 leaves. ‘Pungwonmi’ produced similar to root number 3.9 at –0.1 MPa compared to control, but root weight decreased 75% at - 0.1 MPa more than control. Root did not grow at – 0.2 MPa for ‘Sinjami’. In nursery bed culture condition, roots fresh weight, number of leaves, and shoot length were inhibited according to the decreases of water osmotic potential. In the case of leaf area, ‘Jinhongmi’ and ‘Daeyumi’ was a significantly difference to 8.4, 22.6 cm3 at –0.2 MPa compared to control, respectively. No difference were obsered for ‘Dahomi’ at -0.1 and –0.2 MPa. ‘Pungwonmi’ was decreased 86% at -0.1 MPa compared to the control. ‘Juhwangmi’ and ‘Sinjami’ was a significantly difference to 15.5, 3.6 cm3 at –0.1 MPa compared to control of 22.9, 16.8 cm3, respectively.

본 실험은 ‘설향’ 딸기에서 정식날짜와 정식전 단근처리 및 정식후 굴취처리가 생육, 출뢰율 및 수확량에 미치는 영향을 조사하였다. 60~70일 묘령의 모종을 9월 7 일, 9월 14일, 9월 21일 정식하였고 단근처리는 전체 뿌리의 25%, 50%를 각각 잘라내어 무처리와 비교하였다. 굴취처리는 9월 7일 정식한 묘를 10일후 굴취한 다음 재정식한 것과 20일후 굴취하여 재정식한 것을 비교하였다. 정식일이 늦을수록 지상부 생육이 억제되고 수량이 감소하였다. 정화방과 액화방 출뢰는 다소 앞당겨지는 경향이었다. 단근처리와 굴취처리는 지상부 생육이 억제되고 수확량이 감소하는 경향이었고 25% 보다 50% 처리구에서 더욱 확연하게 보였다. 단근처리시 정화방과 액화방 출뢰일은 무처리에 비해 늦추는 경향을 보인 반면 정식 20일후 굴취처리에서 액화방 분화를 약 7일 정도 앞당길 수 있었으나 수확량은 무처리에 비해 낮은 결과를 보였다. 따라서 액화방의 분화를 앞당기기 위해서는 강한 근권부 스트레스가 필요할 것으로 판단되나 수량과 생육을 낮추는 요인으로 작용하므로 재배시 신중한 판단이 요구된다.

In the present study, the plantlets in vitro of Paeonia suffruticosa ‘Wu Long Peng Sheng’ were used as laboratory materials. The proteome during adventitious root induction process was investigated to sift the related proteins by two-dimensional electrophoresis and mass spectrometry. The results indicated that the protein spots were concentrated in the acidity gel region (pH 4 - 7) and the spots number had a dynamic change ranged from 373 to 462 at the process of root induction (0 – 7 d). 8 spots significantly changed were analyzed with a mass spectrometer and identified using associated software and databases. The peptide information of the 8 spots was similar to the ATP synthase β-subunit of P. suffruticosa (Spots 1 - 4 and 8), P. tenuifolia (Spots 5), P. californica (Spot 6) and P. brownie ( Spots 7) r espectiv ely. T he expression levels of protein spots 1, 4, 5, 6 and 7 was dramatically downregulated, and that of protein spots 2 and 3 had a slightly opposite tendency on the 3rd day. The obviously decreased period is particularly interesting as it was consistent with the induction period of adventitious root primordial of tree peony plantlet in vitro. The ATP synthase β-subunit could be consumed for assembling the ATP synthase in order to supply energy to the rooting process. Therefore, we speculated that the ATP synthase β-subunit was involved in adventitious root initiation of tree peony plantlets in vitro and we expect that further studies should be carried out in order to export its action mechanism.

The most common method of vegetative propagation of virus free plantlets is the use of shoots meristem culture in solid medium culture. This study was to investigate the effect on liquid medium culture for the growth of virus-free sweetpotato plantlets. Single-nodes derived from meristem culture of sweetpotato was examined in this experiment and three sweetpotato varieties ‘Singeonmi’, ‘Sinhwangmi’, and ‘Sinjami’ was used. The growth of plnatlets was greater in liquid medium culture than that of solid medium culture after longer incubation in 3 varieties. The total fresh weight of 5 week old plantlets after planting in solid culture were 2.17 g (‘Singeonmi’), 2.49 g (‘Sinhwangmi’), and 2.18 g (‘Sinjami’), but the fresh weight in liquid medium culture was 3.87, 3.88, and 3.35 g, respectively. Leaf number of ‘Singeonmi’ and ‘Sinjami’ plantlets after 5 weeks of liquid medium culture was 21.1 and 22.6, respectively and liquid medium culture showed 3 and 6.2 more leaf number than that of solid medium culture. Plant height of ‘Sinhwangmi’ and ‘Sinjami’ plantlets after 3 weeks of liquid medium culture was 4.1 cm and 3.4 cm, respectively, and liquid medium culture showed 1.1 cm longer stem length than solid medium. Overall, liquid medium culture of sweetpotato plantlets was more effective than solid medium in terms of leaf and stem growth.

강원도에서 육성한 유색칼라 ‘Lip Glow’와 외국품종인 ‘Captain Safari’ 조직배양묘의 최적 순화 기술 개발로 종구 자 급화 및 농가 실질 소득 증대에 기여하고자 본 연구를 실시하 였다. 순화실은 하우스 형태(1.2 × 6 × 1.5m)이며, 차광(0, 50%)과 흑색차광망(50, 90%) + PE 필름으로 밀폐한 것을 비 교하였다. ‘Lip Glow’의 순화는 흑색차광망 90% + PE 필름 처 리에서 23일째부터 초장이 가장 크고 엽수가 많았고 이후 생 육속도도 빨랐다. 정식 후 45일째의 근수가 많고 근장도 길었 으며, 생체중은 무차광의 1.6배, 건조중은 1.4배 무거웠고, 괴 경 생존율은 81.1%로 무차광 34.2%에 비해 우수하였다. 유색 칼라 ‘Captain Safari’의 순화는 흑색차광망 50% + PE 필름과 흑색차광망 90% + PE 필름 처리에서 23일과 30일째 초장이 크고 엽수가 많았고 이후 생육속도도 빨랐다. 정식 후 45일째 흑색차광망 50% + PE 필름 처리의 초장이 가장 크고 및 엽장 도 가장 많았다. 정식 후 61일째 차광 50% + PE 필름 처리에 서 근수 8.9개로 가장 많고, 근장 13.1cm로 가장 길고, 생체중 5.6g로 무차광의 1.9배, 건조중 1.0g로 무차광의 2.6배 더 무 거웠다. 수확 후 괴경중은 차광 50% + PE 필름 처리에서 5.6g으로 가장 무겁고, 구주도 6.7cm로 가장 컸으며, 생존율 60.6%로 가장 높았지만 차광 50%와 차광 90% + PE 필름 처 리와 유의차는 없었다. 따라서 유색칼라 조직배양묘의 최적 순화 기술로 국내품종인 ‘Lip Glow’는 차광 90% + PE 필름 처 리가, 외국품종인 ‘Captain Safari’ 는 PE 필름 여부와 관계없 이 차광 50% 이상 처리가 효과적이었다.

본 연구는 묘의 생육을 최대화하기 위하여 생육 단계를 임의로 구분하고 각 단계 별 적정 환경 조건을 확립함에 목적을 두었다. 생육 단계는 총 20일의 배양기간을 6일(1단계), 7일(2단계), 7일(3단계)의 3단계로 구분하였다. 첫 번째 단계는 활착기로서 환경 처리 별 생육에 큰 차이가 나타나지 않았다. 높은 환경 조건에 의한 잎의 장해를 고려하였을 때, 80μmmol·m-2·s-1 의 PPFD 및 대기 중의 CO2 농도가 적합하였다. 두 번째 단계에서는 PPFD 및 CO2 조건이 높아짐에 따라 건물 중을 중심으로 부분적으로 향상되었다. 에너지 효율과 생육을 고려할 때, 160μmmol·m-2·s-1 의 PPFD와 700μmmol·mol-1의 CO2가 적합할 것으로 생각되었다. 세 번째 단계에서는 PPFD 및 CO2 농도가 높아짐에 따라 유의적으로 생육이 향상되었으며, 잎 및 마디의 발달상태도 현저히 향상되었다. 따라서 보다 적극적으로 생육증진을 고려할 때, 320μmmol·m-2·s-1 PPFD와 1800μmol·mol-1의 CO2가 적합할 것으로 생각되었다. 생육 단계별 환경 조절은 초기단계에 상대적으로 낮은 조건을 유지하고 후기단계에서 충분한 조건을 제공함으로써 건전한 묘를 생산할 수 있고 에너지 및 물질의 투입량을 절약할 수 있다.

Potato (Solamum tuberosum 'Dejima') plantlets were investigated on culture type and initial quantity of inoculation in bioreactor and survival rate by hydroponics for mass production. rode stems (1 to 1.5cm in length) of potato plantlets multiplied in vitro were grown for 3 weeks in liquid Murashige and Skoog (MS) medium with sucrose 30 g L-1. When plantlets (80-node inoculation) were raised in 10L balloon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured in ebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of 20L BB bioreactor was 120 pieces of stem segments (mean 2.2g fresh weight) in ebb & flow culture. Number of nodal shoot was eight on the average. In controlled culture room, survival rate of plantlets at 7 days after stem cutting was above 70% when they were acclimatized by hydroponics grown in deep flow and solid medium culture. The highest survival rate of the stem cutting plantlets was in nutrient solution adjusted to EC 1.4dS·m-1. Stem cutting plantlets through one culture could be obtained 670~900, when plantlets were grown in ebb & flow culture during 3 weeks using a 20L bioreactor with initial 120 pieces of nodal segments. 11 is possible In do mass production of seedlings cultured in bioreactor and hydroponics.

자주초롱꽃(Campanula punctata 'Rubriflora')의 자가영양배양시 CO2 농도,환기횟수 및 광도가 소식물체의 생장에 미치는 영향을 조사하였다. 1,500μmol·m-1고농도CO2에서는 광도를 높일수록 초장, 엽수가 증가되었으나, 최대근장은 유의성이 인정되지 않았다 엽록소의 함량은 광도가 높을 때보다는 낮은 경우인 70μmol·m-2s-1에서 가장 많이 증가되었다 엽면적은 광도가 높을수록 작아지는 경향을 보였으며, CO2 1,500μmol·mol-1 환기횟수 2.8h-l, PPF 220μmol·m-2·s-1에서 가장 큰 차이로 작게 나타났다. 생체중과 건물중은 CO2 농도, 환기횟수, PPF가 높을수록 증가되었다. 특히 CO2 1,500μmol·mol-1, 환기횟수 2.8h-1, PPF 220μmol·m-2·s-1에서 가장 크게 증가되었다. 환기횟수의 영향은 전반적으로 적었다. 종합적으로 자주초롱꽃 소식물체의 기내 자가영양배양 차량번식을 위해서는 CO2 1,500μmol·mol-1, PPF 220μmol·m-2·s-1의 조건이 적합하였다.

자가영양배양시 광도,환기횟수 및 CO2농도가 스타티스 ‘Ocean Blue’ 소식물체의 생장에 미치는 영향을 조사하였다 MS 배지에서 광흔합영양해양을 통하여 증식된 스타티스 식물체에서 2~3장의 잎을 가진 줄기 절편체를 채취하여 배양용기당 4개씩 치상하였다. 절편체는 온도 25±1℃. 상대습도 70~ 80%인 배양실에서 cool-white 형광램프로 명기동안 70, 150, 또는 220μmol. m-2 . s-1의 광을 공급하면서 41일 동안 배양하였다. MS 기본배지를 사용하였으며, sucrose와 vitamin은 첨가하지 않았다. 광도가 높아질수록 생체 중,건물중 및 엽면적이 증가하였다. CO2농도를 높여준 경우 초장, 생체중, 건물중 및 엽면적이 증가하였다. 뿌리길이와 엽록소 농도는 광도나 CO2의 영향을 받지 않았다. 배양용기 내의 환기횟수를 높이고, 고광도의 CO2를 공급할수록,그리고 PPF를 증가시킬수록 스타티스 소식물체의 자가영양배양에서의 생육이 촉진되었다.

본 연구는 배양기의 환기횟수(0.1 h-1 또는 2.8 h-1), 광도(70, 150 또는 220μmol. m-2 . s-1), C O2 농도(400-500, 1000 또는 2000μmol.mo l-1)가 국화 소식물체의 기내생장에 미치는 영향을 조사하기 위하여 실시하였다. MS 배지에서 혼합영양배양 상태 하에서 번식시킨 줄기 마디 절편체를 채취하여 1.25meq. L-1의 H2S O4-가 추가로 첨가된 MS 기본배지가 담긴 3.7×10-4 m3의 배양기에 용기당 4개씩 치상하였다. 환기횟수(NAEH)를 0.1 h-1에서 2.8 h-1로 향상시키기 위하여 배양용기의 뚜껑에 직경 10mm의 구멍을 뚫어 통기성의 필터를 부착하였으며, 당과 생장조절제는 첨가하지 않았다. 배양실에 C O2를 공급하기 위하여 공기 순환통로에 C O2 가스 공급구를 설치하여 액화 C O2 가스를 명기 동안에만 공급해 주었다. 생체중과 건물중, 초장, 최대근장, 엽수, 엽면적은 광도(PPF)가 높은 처리구에서, 특히 C O2 농도가 높을 때 증가되었다. 그리고 환기횟수가 낮은 처리구에서보다 높은 처리구에서 더 컸으며, 처리효과는 배양후기로 갈수록 뚜렷이 증가되었다. 국화 소식물체의 기내생육은 환기횟수가 2.8 h-1, PPF가 220μmol. m-2 . s-1, 그리고 C O2 농도가 2000μmol.mo l-1일때 가장 빨랐다.

Background : Oplopanax elatus, comnonly called Asian devil’s club, is distributed in the northern high mountains of the Korean peninsula, northeastern China, and southeastern Russia. Its root is traditionally used to treat asthma, depressive states, chronic fatigue syndrome, and hypertension, neurasthenia, hypopiesis, schizophrenia, cardiovascular problems, diabetes mellitus, impotence, emaciation, hypotension cough, rheumatism, arthritis, gastrointestinal disorders, and wounds. Plant cell and tissue culture techniques for the mass production of adventitious roots in suspension culture allows a high proliferation rate, stable accumulation rate, and production of valuable compounds. Methods and Results : The plants were cultured on 1/4 MS medium with 1% sucrose and 1% plant agar for 3 month and they transferred to pot. The pots were contained of soil, soil + pelite (1 : 1), and soil + sand (1 : 1), respectively. The plants were measured plant length, leaf length, and leaf number. The number of leaves have represented as similar result of 7.44 ± 1.34 ㎝ in soil and 7.56 ± 1.26 ㎝ on soil + pelite. The 3.22 ± 0.53 ㎝ of plant length and the 6.57 ± 0.51 ㎝ of leaves length were highest on soil. Conclusion : The survival rate depending on soil types of Oplopanax elatus was confirmed to exist in these cases of soil and soil+pelite. However, they all died in condition of soil+vermiculite. The number of leaves have no difference and the length of plant and the length of leaves were longest on soil.

Background : Momordica charantia L. (M. charantia) is a member of the family Cucurbitaceae, used as a medicine herb in traditional medicine. In this study, we present the sequencing, de novo assembly and analysis of the transcriptome of M. charantia and provide a global description of relationship between putative phenylpropanoid and flavonoid biosynthesis genes and alteration of phenylpropanoid and flavonoid content during different organs and plantlet of M. charantia. Methods and Results : The transcriptome of M. charantia was constructed by using an Illumina Nexteseq500 sequencing system. Out of 68,073,862 total reads, approximately 88,703 unigenes were identified with a length of 898 bp. Alternatively, transcriptomic data, 10cDNAs (McPAL, McC4H, Mc4CL, McCOMT, McCHS, McCHI, McF3H, McFLS, McDFR and Mc3GT) encoded phenylpropanoid and flavonoid biosynthetic genes. The expression levels and the accumulation of trans-cinnamic acid, benzoic acid, 4-hydroxyvbenzoic acid, p-coumaric acid, chlorogenic acid, caffeic acid, catechin hydrate, ferulic acid, and rutin were investigated in different organs and plantlets. Mainly, phenylpropanoids and flavonoids accumulated in leaves and flowers, whereas low levels accumulated in roots. Collectively, these results indicate that the putative McPAL, McC4H, McCOMT, McFLS, and Mc3GT might be key factors for controlling phenylpropanoid and flavonoid contents in M. charantia. Conclusion : In this study, we present the sequencing, de novo assembly and analysis of the transcriptome of M. charantia. We also compared gene expression and compound analysis of phenylpropanoid and flavonoid in different organs and plantlet of M. charantia. These results indicate that McPAL, McC4H, McCOMT, McFLS, and Mc3GT are key regulators of phenylpropanoid and flavonoid accumulation in M. charantia

The present study aimed to determine the influence of various root restriction media on seedling quality and early growth of strawberry after transplanting. The root activity of the seedlings, measured 20 days after fixation, was considerably higher (0.096, 0.090, and 0.063 mg·g-1·h-1 at 420, 450, and 480 nm, respectively) in expanded rice hull (ERH) treatment than in the sandy loam and loamy sand treatments. The volumetric water content (VWC) of the root media tested across 3 irrigation regimes (15 d, 30 d, 45 d) in the nursery field was highest in sandy loam (65.0–66.8%), followed by 59.4–61.3% in loamy sand and 38.6–45.3% in ERH. When growth parameters of runner plantlets were compared, ERH treatment was found to result in the highest crown thickness and fresh weights of root and above-ground parts. This had a favorable influence on above-ground tissue growth after transplanting to plastic house soil. As mentioned above, ERH treatment resulted in the highest seedling quality and early growth after transplanting. The results of this study would serve as useful on-site data for the production of high-quality strawberry seedlings.

The effects of red, blue, and far-red light by illumination of light emitting diodes (LEDs) on growth, morphogenesis and eleutheroside contents of in vitro plantlets of Eleutherococcus senticosus were examined. As a control, plantlets were grown under a broad spectrum white fluorescent lamp (16/8 h illumination). The length of plantlets grown under the red/blue LEDs was taller than those under fluorescent lamps. Leaf area, root length and fresh weight of plantlets were highest under blue light compared to other kinds of light sources. Chlorophyll contents in plantlets grown under fluorescent lamps were higher than those in plantlets grown under LED illumination. Production of eleuthroside B and E in plantlets was highest under blue LED. However, production of eleuthroside E1 was highest under fluorescent lamps. These results suggest that plant growth and eleuthroside accumulation can be controlled by wave length of light under LED illumination system.