간행물
한국응용곤충학회 학술대회논문집
- 발행기관 한국응용곤충학회
- 자료유형 학술대회
- 간기 반년간
- 수록기간 2000 ~ 2024
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 495DDC 595
권호리스트/논문검색
2018 한국응용곤충학회 춘계학술발표회 및 국제컨퍼런스 (2018년 4월) 299건
Poster General entomology P061
161.
2018.04
구독 인증기관·개인회원 무료
Drosophila melanogaster and Drosophila suzukii are the species of the family Drosophilidae. Although these two fruit flies are taxonomically close species, D. suzukii is thought to be evolutionally adapted to the flesh or maturing fruits, whereas D. melanogaster is adapted to more fermented environments. According to the previous studies, several environmental toxins, such as acetic acid, ethanol, methanol and phenylacetate, ect., have been identified from rotten fruit and fermentation procedures. Considering the differences of distinct habitat between two flies, D. melanogaster is hypothesized to exhibit higher tolerance to the chemical toxins than D. suzukii. Therefore, in this study, we compared the tolerance and susceptibility of two fruit flies to three chemicals (acetic acid, ethanol, 2-phenlyethano).
Poster General entomology P062
162.
2018.04
구독 인증기관·개인회원 무료
Although the white-spotted flower chafers beetle, Protaetia brevitarsis, receive attention to explore useful active ingredient for the preventive effect of liver-related diseases, little is known about cosmetic effect. Here we report that the larva extract of the white-spotted flower chafers has functional cosmetic effect using the B16F10 melanoma cell and HaCaT keratinocyte cell. The ethanol extract of hot-air dried P. brevitarsis larvae showed the inhibitory activity of tyrosinase (an enzyme that regulates the melanin production) and remarkably inhibit melanin biosynthesis in the B16F10 melanoma cell. Further, the ethanol extract induced the expression of hyaluronic acid synthetase (HAS2/HAS3), which produce hyaluronan related to epidermal structure, and indeed increased hyalurona in HaCaT keratinocyte cell. Consequently, P. brevitarsis could be new functional cosmetic material for whitening and moisturizing effect.
Poster General entomology P063
163.
2018.04
구독 인증기관·개인회원 무료
The effects of temperature on starvation resistance are context dependent in Drosophila melanogaster
Temperature can modulate how insects respond to environmental stressors, such as starvation. In this study, we examine whether and how the effects of temperature on starvation resistance depend on nutritional condition and developmental stages in Drosophila melanogaster. Starvation resistance decreased as the temperature exposed during starvation rose from 18 to 28 ̊C, which was mainly caused by warming-induced increase in energy expenditure. When exposed to warm temperatures during feeding, D. melanogaster accumulated more energy reserves and thus become more starvation resistant. The temperature experienced during the larval stage also had a significant effect on starvation resistance at adult stages, with those larvae raised at cold temperatures developing into adult phenotypes with reduced resistance to starvation. This study suggests that the effects of temperature on starvation resistance are highly complex and context dependent in D. melanogaster.
Poster General entomology P064
164.
2018.04
구독 인증기관·개인회원 무료
Protein and carbohydrate are two major macronutrients that exert profound influences over fitness in many insects, including Drosophila melanogaster. Until recently, most studies examining the impacts of these macronutrients on various life-history traits in this species have used semi-synthetic diets that are not nutritionally well-defined. Here we used chemically defined diets to examine the patterns of larval and adult traits expressed across 34 diets systematically varying in the ratio and concentration of protein and carbohydrate. The shapes of the nutritional landscapes plotted for all larval and adult traits differed significantly from one another. Diverging nutritional optima identified for these landscapes suggest that D. melanogaster cannot maximize the expression of all life-history traits simultaneously, thus leading them to face a nutrient-dependent life-history trade-off.
Poster General entomology P065
165.
2018.04
구독 인증기관·개인회원 무료
Diets influence lifespan and reproduction in insects, but little is known how temperature modulates the impacts of diet on these two key fitness components. Here we examined the interactive effects of temperature and nutrient balance on lifespan and egg production rate in Drosophila melanogaster. Newly emerged adult D. melanogaster were allowed to feed ad libitum on one of eight chemically defined diets differing in P:C ratio (1:16, 1:8, 1:4, 1:2, 1:1, 2:1, 4:1, or 8:1) under one of six ambient temperatures (13, 18, 23, 28, 31, or 33℃). For both males and females, lifespan was longest for D. melanogaster fed on P:C 1:16 diet at 13℃ and shortened as both temperature and P:C ratio increased. As indicated by a significant temperature-by-diet interaction for lifespan, the diet effects on lifespan were more pronounced at lower temperatures than at higher temperatures. Egg production rate was maximized on P:C 4:1 diet at 28℃.
Poster General entomology P066
166.
2018.04
구독 인증기관·개인회원 무료
G protein-coupled receptors (GPCRs) belong to cell membrane protein family, which regulate various physiological process such as reproduction, behavior and immune etc. In other to identify the GPCRs in pheromone gland of Maruca vitrata, we carried out transcriptome analysis from both females. Transcriptome analysis in the pheromone gland yielded approximately 22Gb and 47,528 transcripts showed positive FPKM value. 48 Genes involved in GPCRs were identified such as pheromone biosynthesis activating neuropeptide receptor (PBANr), prostaglandin receptors, neuropeptide receptor, 5-hydroxytryptamine receptor, galanin receptor, calcitonin gene-related peptide receptor, diuretic hormone receptor, gonadotropin-releasing hormone receptor, frizzled and orphan receptors, etc. Various expression of GPCRs in the pheromone gland indicates the role of pheromone gland may not be limited to the production of pheromone.
Poster General entomology P068
167.
2018.04
구독 인증기관·개인회원 무료
외래 병해충의 유입이 증가 하고 있고 이들의 유입 및 확산 경로 규명을 위하여 분자 유전학적 분석 방법이 이용되고 있는 실정이며, 본 연구는 외래 해충인 Reticulitermes kanmonensis의 유전적 특성을 분석하기 위한 초위성체마 커(microsatellite)를 문헌을 통해 탐색하였고, NGS(Next-Gen Sequencing) 기술을 통해 새로운 초위성체마커를 개발하였 다. 문헌을 통해 Reticulitermes속에서 개발된 마커29개와 NGS를 통해 선발된 25개의 마커를 탐색 하여, 이중 증폭 및 유용성이 있는 18개의 마커를 선정하였다. 선정된 단일 마커들을 이용하여 6개의 multiplex PCR set 및 증폭 조건을 수립하여 집단유전학 분석에 활용하고자 한다.
Poster General entomology P067
168.
2018.04
구독 인증기관·개인회원 무료
Ju Hyeon Kim, Chae Eun Hwang, Kyungjae Andrew Yoon, Keon Mook Seong, Jonghwa Lee, Jeong Han Kim, Si Hyeock Lee
The common bed bug, Cimex lectularius, possesses a cholinesterase expressed exclusively in the salivary gland (ClSChE). In this paper, we investigated the molecular structure, tissue distribution patterns, and biochemical properties of ClSChE and showed that ClSChE exists as a soluble monomeric form or a soluble dimeric form connected by a disulfide bridge. Immunohistochemical analysis confirmed that ClSChE was expressed in the epithelial cells of both the salivary gland and the duct. In addition, the secretion of monomeric ClSChE through the proboscis during feeding was detected by western blotting using a ClSChE-specific antibody. To predict the role of ClSChE injected into the tissue of an animal host, we analyzed the extent of sequestration and hydrolysis of acetylcholine (ACh)/choline (Ch) by ClSChE by ultra-performance liquid chromatography-tandem mass spectrometry. Kinetic analysis revealed that ClSChE possesses extremely low Km (high affinity to ACh) and Vmax values. These findings suggest that ClSChE functions as a sequestering enzyme specific to ACh (not to Ch) by having a very strong affinity to ACh but an extremely long turnover time.
Poster General entomology P070
169.
2018.04
구독 인증기관·개인회원 무료
Maruca vitrata is one of the destructive pests of tropical legumes. Radiofrequency dependent development and adult performances of M. vitrata were examined at five radiofrequencies of 0 (control), 5, 10, 20, and 30 kHz on artificial diet. The results showed that radiofrequency have significant effects on the life variables of M. vitrata. Radiofrequency exposures on pod borer impacted on life-variables of M. vitrata and negative effects were also transmitted to the successive generations. The adults of M. vitrata were emerged with shorter longevity in case of radio-frequency treatment than untreated. Decreasing the radiofrequency level further reduced the longevity of adults. The shortest adult longevity was recorded for 5 kHz. Radiofrequency treatments have also affected on the adult performance such as weight of adult. The outcomes of this study are discussed in terms of an alternative to chemical treatments.
Poster General entomology P069
170.
2018.04
구독 인증기관·개인회원 무료
Harmonia axyridis have become extremely popular through their extensive elytra and spot variation for many years. However, phenotypic polymorphism and striking visual phenotypes are not yet to be extensively studied. With growing attention to polymorphism of H. axyridis, it is imperative to study involved in genomic research in order to elucidate the molecular mechanisms underlying the diversity of elytra variation. To figure out suitable approach for analyzing genes of H. axyidis, we assessed RNAi as a tool for functional analysis. In order to demonstrate the utility of RNAi technique on H. axyridis, we conducted cDNA library screening to affirm silencing effect. We choose random genes from cDNA clones and the random genes were synthesized into dsRNA. After the injection of dsRNA into last instar larvae, we observed morphological defects or deaths. To investigate the effectiveness of knockdown in H. axyridis, we performed quantitative real time PCR on H. axyridis that appears the most distinctive features. These results provide that RNAi is highly applicable in H. axyridis and it will be useful for the gene functional analysis.
Poster General entomology P071
171.
2018.04
구독 인증기관·개인회원 무료
Nosema disease caused by the microsporidia Nosema apis and Nosema ceranae are a honey bee pathogen parasitizing. Nosema disease symptoms include digestive and absorption disorders because the spores damage epithelial tissue and potentially causing colony death. Recently, N. ceranae has been reported as an important threat to honey bee health. Turmeric (Curcuma longa L.) Curcuma tonga L. belongs to the family Zingiberaceae and is a perennial, tropical herb. Turmeric, the powdered rhizome, is used for medicinal purposes. The aim of this study was to evaluate the potential of Turmeric (Curcuma longa L.) for the control of N. ceranae in honeybees. For the study, we infected with N. ceranae spore through dosed and fed with the turmeric extraction at difference concentration. The data show that the turmeric extraction was not toxic for bee at least at 1% and the bees fed with 0.5 % turmeric extraction had significantly lower infection rates. This data suggest that turmeric could be useful in alternative strategies for the control of N. ceranae.
Poster General entomology P072
172.
2018.04
구독 인증기관·개인회원 무료
약제 처리에 따른 담배거세미나방(Spodoptera litura) 유충의 섭식 행동 및 반응을 조사하기 위해 1분 간격으로 잎면적을 자동 측정하였다. 담배거세미나방 유충의 섭식 행동은 섭식기와 비섭식기가 뚜렷하게 구분되었으며, 섭식기가 끝나면 비섭식기를 거쳐 탈피를 하고 다시 섭식기에 들어갔다. 1회 섭식 지속 시간은 약 15분으로 조사되었고, 영기 내 평균 섭식 회수는 약 27.5회로 섭식 지속 시간과 마찬가지로 영기에 따른 차이는 없었다. 약제 처리는 피망 잎을 Buprofezin methoxyfenozyd과 BT (Bacillus thuringiensis) 500ppm 약액에 10초간 침지 처리하였다. Buprofezin methoxyfenozyd를 처리 하였을 경우 섭식 시간은 평균 8~9분으로 감소하였고, 치사 전까지 섭식량은 각각 176mg으로 조사되었다. 하지만 BT제를 처리 시에는 담배거세미나방 유충은 치사하지 않았다.
Poster General entomology P073
173.
2018.04
구독 인증기관·개인회원 무료
Ra Mi Woo, Jong Hoon Kim, Jae Young Choi, Dong Hwan Park, Min Gu Park, Bo Ram Lee, Jae Su Kim, Soo Dong Woo, Yeon Ho Je
The development of entomopathogenic fungi has received increasing interest as part of integrated pest management strategies as biocontrol agents. It is reasonable to assume that entomopathogenic fungi might produce secondary metabolites modulating juvenile hormone for their survival against defense mechanisms of host insects. In this study, acetone extracts of 189 entomopathogenic fungi cultured on unpolished rice medium were screened for their juvenile hormone antagonist (JHAN) activities using the yeast-two hybrid system. Among 14 extracts showing JHAN activities, extract of the F-145 showed high level of insecticidal activities against both Plutella xylostella and Aedes albopictus. This isolate was identified as Lecanicillium attenuatum. These results suggested that the Lecanicillium attenuatum could be useful for development of eco-friendly insecticides.
Poster General entomology P074
174.
2018.04
구독 인증기관·개인회원 무료
Beet armyworm, Spodoptera exigua, is known to be hard-to-kill pest by having high resistance to insecticides and its control is intensely dependent on fewer insecticides. In this study, the ethyl acetate extract of Streptomyces sp. was evaluated to find novel insecticidal agents against S. exigua. In order to determine the identity of insecticidal compounds, the crude ethyl acetate extract was fractionated based on the TLC profiling and bioassay-guided monitoring. These results indicated that the non-polar fraction have high level of insecticidal activity against second instars of S. exigua. These findings suggested that secondary metabolites produced by Streptomyces sp. could be considerable potential resources as novel insecticidal formulation candidates.
Poster General entomology P076
175.
2018.04
구독 인증기관·개인회원 무료
One of the leading pests of rice, small brown planthopper (Laodelphax striatellus) can grow up to have either short or long wings, depending on conditions. However, under the same breeding conditions, the phenotypes of the long- and short-winged small brown planthopper observed to keep the first collected phenotype. To investigate the mechanism involved in wing dimorphism, metabolomic researches have been conducted. In this study, we observed several metabolites change, and the difference of metabolites could provide clues to the relationship between physiological changes in the small brown planthopper and ecological transport.
Poster General entomology P075
176.
2018.04
구독 인증기관·개인회원 무료
Streptomyces has been reported to produce various secondary metabolites which have the potential to become environmentally safe insecticides. In this study, 1,274 streptomyces culture filtrates were screened for their JHAN activity in order to identify novel insecticidal compounds. 34 isolates with high levels of JHAN activity were selected, and their insecticidal activities were tested against Plutella xylostella larvae. Among them, IMBL-263 which was revealed to be Streptomyces anulatus by 16s rRNA sequencing showed the highest insecticidal activity. Also, systemic activities of secondary metabolites extracted from the S. anulatus on plant, Brassica napus, were investigated. These results suggested that secondary metabolites from the S. anulatus might be useful for development of novel environmentally benign insecticides.
Poster General entomology P078
177.
2018.04
구독 인증기관·개인회원 무료
Hee Geun Park, Kwang Sik Lee, Bo Yeon Kim, Hyung Joo Yoon, Yong Soo Choi, Kyung Yong Lee, Byung Rae Jin
Honeybee (Apis mellifera) egg-yolk protein vitellogenin (Vg) plays roles in immunity, antioxidation, and life span beyond reproduction, but it also acts as an allergen Api m 12 in venom. Here we established antimicrobial and antioxidant roles of honeybee Vg in the body and venom. Using the cDNA encoding Vg identified from Asiatic honeybee (A. cerana) workers, recombinant A. cerana Vg (AcVg) protein of approximately 180 kDa was produced in baculovirus-infected insect cells. In A. cerana worker bees, AcVg was expressed in the fat body and venom gland and was present in the secreted venom. AcVg induced structural damage in microbial cell walls via binding to microbial surfaces and exhibited antimicrobial activity against bacteria and fungi. AcVg protected mammalian and insect cells against oxidative damage through direct shielding of cell membranes. Interestingly, AcVg exhibited DNA protection activity against reactive oxygen species (ROS). Furthermore, the transcript level of AcVg was upregulated in the fat body, but not in the venom gland, of worker bees with antimicrobial peptides and antioxidant enzymes in response to microbial infection and oxidative stress. Our data indicate that AcVg is involved in innate immunity upon infection and in a defense system against ROS, supporting a crucial role of honeybee Vg as an antimicrobial and antioxidant agent in the body and venom.
Poster General entomology P077
178.
2018.04
구독 인증기관·개인회원 무료
Artificial light is frequently used for plant growth and pest attraction traps in greenhouses. In addition, natural enemies may utilize this artificial light for colonization in greenhouses. Orius laevigatus is a biological control agent of whiteflies and thrips. The Y-tube selection test was conducted against O. laevigatus for 11 wavelengths (365, 385, 395, 405, 410-420, 420-430, 440-450, 490-500, 520-530, 590-595 and 620-630 nm) of LED and white light (5000k) for a control . One pipe of the Y-tube was allocated for insect entrance and other two pipes of the Y-tube were installed transparent sticky trap along with white LED and test wavelength, respectively. At least, three replications were conducted for each wavelength and 40 to 60 individuals of O. laevigatus were used for each replication. Attraction rate of 365 to 405 nm wavelength was above 50 % and highest at 385 nm (83.7 %). Attraction rate of 490 to 630 nm was below 20 % and lowest at 620-630 nm (1.4 %). The results indicate that O. laevigatus attracted most to 385 nm wavelength and this light may be useful for using O. laevigatus in green houses. Further research is needed using selected LED wavelength.
Poster General entomology P080
179.
2018.04
구독 인증기관·개인회원 무료
우리나라는 약 4,000여개의 크고 작은 섬들로 이루어져 있고, 그 속에는 다양한 생물종들이 주변 환경과 상호작용하 며 서식하고 있다. 그 중 초식성 곤충은 생물 구성의 1/4로 많은 비율을 차지하며, 생산자인 식물을 먹고 2차 소비자의 먹이원이 됨으로써 생태계의 중간 고리 역할을 한다. 본 연구는 다도해해상국립공원에 속해 있는 크기가 다른 6개의 섬에서 초식성 곤충의 영향을 알 수 있는 식흔과 여기에 영향을 줄 수 있는 환경요인(섬 면적, 육지와의 거리, 해안선 길이, 최고고도)과의 관계를 알아보았다. 식흔을 알아보기 위해 낙엽활엽수인 4개의 수종(참나무속, 벚나무속, 예덕나무속, 오리나무속)을 선정하여 2017년 6월과 9월에 관찰하였다. 이와 함께 초식성 곤충 중 종 다양성이 풍부한 나방을 채집하여 식흔자료와 비교하였다. 조사결과 섬 면적이 커질수록, 해안선의 길이가 길수록, 최고고도가 높을수록 식흔량이 증가하는 경향을 보였고, 나방의 종수도 증가하였다. 반면 육지와의 거리가 멀수록 식흔량이 감소하는 경향을 보였고, 나방 종수도 감소하였다. 이러한 결과를 통해 초식성 곤충의 다양성과 환경요인이 관련 있는 것을 확인할 수 있었다
Poster General entomology P079
180.
2018.04
구독 인증기관·개인회원 무료
Honeybee (Apis cerana) Major Royal Jelly Protein 2 Exhibits Antimicrobial and Antioxidant Activities
Major royal jelly proteins (MRJPs), important protein components of bee royal jelly (RJ) and exclusive nourishments for queen, exhibit various biological and pharmacological activities. RJ is one of the most studied bee products, but the crucial roles for MRJP2 as an antimicrobial and antioxidant agents remain largely unknown. Here we demonstrated the antimicrobial and antioxidant functions of the Asiatic honeybee (Apis cerna) MRJP2 (AcMRJP2). Recombinant AcMRJP2 of approximately 53 kDa was expressed in baculovirus-infected insect cells, and it exhibited antimicrobial activity against bacteria, fungi, and yeast via binding to microbial surfaces and inducing structural damage in microbial cell walls. AcMRJP2 protected mammalian and insect cells against oxidative damage through shielding of cell membranes. Interestingly, AcMRJP2 exhibited DNA protection activity and DPPH radical-scavenging activity. Altogether, our data demonstrated that AcMRJP2 functions as antimicrobial and antioxidant agents.
