This study was performed to investigate the possibility of repeated superovulation treatment at interval from 27 days to 41 days in Hanwoo cattle and to compare with superovulation effect between doses of FSH 200 mg and FSH 400 mg. Different doses of FSH (200 mg or 400 mg) were injected at Day 8 after controlled internal drug release (CIDR) treatment for superovulation of Hanwoo donors following CIDR treatment (Day 8 after the estrus). Superovulation was repeated four times for one donor and number of corpus luteum (CL), number of embryos, number of transferable embryos and pregnancy rate after embryo transfer (ET) were investigated. 5 cows were used for each FSH treatment (10 cows in total). Average number of CL were and for the donors treated with FSH 200mg and FSH 400mg, respectively. Average number of embryos collected were and for the donors treated with FSH 200 mg and FSH 400 mg, respectively. Average number of transferable embryos were and for the donors treated with FSH 200 mg and FSH 400 mg, respectively. The pregnancy rate following ET with embryos collected from 200 mg FSH treated donors and 400 mg FSH treated donors were 61.9% and 53.8% respectively. The numbers of embryos tended to be decreased as the numbers of repeat of superovulation were elapsed. These results indicated that superovulation treatment by about a month to Hanwoo donors is usable and 200 mg of FSH is preferable for simple FSH treatment following CIDR treatment.

Oxide effects experiments on massive hydriding reactions of Zr alloy with hydrogen gas were carried out at 400˚C under 1 atm in a H2 environment with a thermo-gravimetric apparatus (TGA). Experimental results for oxide effects on massive hydriding kinetics show that incubation time is not proportional to oxide thickness. The results also show that the massive hydriding kinetics of pre-filmed Zr alloys follows linear kinetic law and that the hydriding rates are similar to that of oxide-free Zr alloys once massive hydriding is initiated. Unlikely microstructure of the oxide during incubation time, physical defects such as micro-cracks and pores were observed in the oxide after incubation time. Therefore, it seems that the massive hydriding of Zr alloys can be ascribed to short circuit paths and mechanical or physical defects, such as micro-cracks and pores in the oxide, rather than to hydrogen diffusion through the oxide resulting from the increase of oxygen vacancies in the hypo-stoichiometric oxide.

This study was performed to investigate the result that in-vivo or in-vitro embryos of Hanwoo cows were transferred to Holstein cows. Seventeen Hanwoo cows were used as donors for production of in-vivo embryos and fresh hanwoo in-vivo embryos were transferred to 1,150 Holsteins. And 2 embryos were transferred to 188 Holstein recipients to produce twin calves. Diagnosis on pregnancy was performed by rectal palpation at days after transfer. The pregnancy rate of Holstein recipients was 55.8% after transfer with Hanwoo in-vivo embryos and 38.2% after transfer with Hanwoo in-vitro embryos. The delivery rate of pregnant Holstein recipients was 88.4% after transfer with Hanwoo in-vivo embryos and 75.6% after transfer with Hanwoo in-vitro embryos. The rate of delivery of Holstein recipients transferred with two Hanwoo embryos was 36.2% and the rate of twin production was 25.9%. The rate of twin production by embryo transfer with in-vivo embryos was 30.4%, whereas the fate with in-vitro embryos was 15.6%. The pregnancy rate according to the grade of corpus luteum of Holstein recipients transferred with Hanwoo in-vitro embryos was 41.5 and 36.0% for A and B grade, respectively. The pregnancy rate according to the transfer in site in the uterine lumen of recipients was 40.9 and 32.7% for anterior and middle site, respectively. The pregnancy rate according to day of embryo transfer after estrus of recipients was 45.5, 38.8 and 39.7% for day 6, day 7 and day 8, respectively. There was difference of pregnancy rate according embryo transfer technician () individual dairy farm (). These results are supposed to indicate that the rate of pregnancy after transfer with Hanwoo embryos to Holstein recipients was similar to that within the same breed, and consequently that this method would be beneficial to enhance the productivity in Hanwoo reproduction.

본 연구의 목적은 수공간에 대한 감성을 대표하는 어휘를 선정과 수공간의 감성 유형을 분석하기 위해 수행되었다. 수공간의 감성평가를 위한 어휘를 선정하는 것은 감정의 잣대를 만드는 일이기 때문에 감성어휘를 정확하게 선정하는 것은 매우 중요한 일이다. 기초어휘 수집을 위해 첫 단계에서 수공간에 대한 감성을 표현하는 어휘를 수공간 연구, 경관 평가 연구, 환경디자인 감성연구 등에서 수집 후 최종 129개의 어휘를 선정하였다 두 번째 단계에서는 수집된 어휘를 일반인들에게 제시하여 수공간의 감성을 나타내는 어휘로서 적절함의 정도를 5점 척도로 표시하도록 하여, 평균 3.5점 이상의 39개 어휘를 선발하였다. 세 번째 단계로 이 설문으로부터 나온 데이터에 대해 요인분석, 군집분석, 다차원분석을 실시하여 수공간에 대한 9개의 감성어휘를 추출하였다. 수공간 감성평가에 최종적으로 선발된 어휘는 체험적, 감각적, 자연적, 조화된, 깨끗한, 기분좋은, 역동적, 개방적, 낭만적이다.

The purpose of this study is to plan, design and reflect to the future naturalization of the urban rivers by using the literatures, field survey results and aerial photography of Milyang River as the case regarding the effect of physical factors of urban rivers on landscape, defining those factors as Sammon fine forest, outdoor stage, Youngnamlu, RC stadium, stroll roads and bicycle roads, artificial island, outdoor swimming pool and sculpture park, and by suggesting the specific characteristics of the landscape according to those factors. The questionnaire survey was made to understand the users' satisfaction and attributes. The statistical analysis was applied, the frequency analysis and percentage calculation were used to find out the citizenship and perception toward the physical factors, and paired-sample T test to compare the average.

한우 및 젖소에서 체외 수정란을 생산하여 수란 우에 이식하기 위해 도축장에서 난소를 채취하여 난자를 회수한 후 체외 성숙, 체외 수정, 체외 배양과정을 거쳐 수정란을 생산하였다. 체외 수정시 난자는 난구세포 부착 정도에 따라 난할율을 조사하였고, 한우와 젖소에서 체외수정 후 배반포율을 조사하였다. 신선 및 동결 수정란을 수란우에 각각 이식하여 산자 분만에 따른 수태율을 조사하였다. 1. 한우 난소 2,021개에서 20,387개의 난자를 회수하여 체외 수

가축에서 생산성을 높이기 위해 사용되는 수정란이식술의 실용성을 알아보기 위하여 한우 및 젖소 36두로부터 과배란 처리를 하여 신선수정란은 25두에, 동결수정란은 17두에, 쌍태 생산을 위한 수정란 두 개 이식은 13두 수란우에 이식되어 그 생산 결과를 조사하였다. 1 과배란을 위한 호르몬 처리후 반응을 나타낸 공란우는 75.0％이었다. 2. 황체수와 비교하여 채란된 수정란 율은 91.8％ 이었다. 3. 비외과적으로 채란된 전체 난자 중 생존성이 있는 배

체외수정된 한우 수정란을 PCR 기법에 의한 성감별을 위해 생검하여 회복시까지 1∼2시간 동안 배양하였다. 이 성감별된 한우 수정란을 2000년 2월부터 2001년 2월까지 한우 49두와 젖소 16두에 이식하였다. 수정란이 이식된 65두의 수란우중 14두(한두 12두, 젖소 2두)가 성감별 결과와 동일한 성의 산자를 생산하였고, 전체 수태율은 21.5%를 나타내었다. 1. 총 65두의 수란우중 웅성 수정란은 35두에 자성 수정란은 30두에 이식되었고 이

This study was carried out to determine the factors on achieving good viability of embryos biopsied fur sexing, to investigate pregnancy rate following embryo transfer(ET) with sexed embryos, and to confirm the accuracy for the calves bort following ET with sexed embryos by polymerase chain reaction(PCR). To investigate viability of Hanwoo embryos after biopsy for sexing, fresh and frozen/thawed embryos were biopsied according to different developmental day of blastocysts, different stage of blastocysts, and different biopsy grade and the embryos themselves were incubated for 2 hours in TCM199 after microsection to be evaluated morphologically for recovery as blastocyst. The results obtained were as follows : 1. The rate of oocytes cleaved in vitro and the rate of blastocyst of the cleaved oocytes were 52.5% and 21.6%, respectively. The rate of blastocyst on day 8 was 11.2%, denoting the highest rate during whole culture period posterior to in vitro fertilization(IVF) 2. After biopsy for sexing, the viability rate of blastocyst on day 7, 8 and 9 was 75.0%, 88.4%, and 100.0%, respectively and the viability of early, mid, and expanded blastocyst after biopsy was 75.0%, 88.9%, and 91.1%, respectively The viability rate of fresh and frozen/thawed embryos was 89.9%, 71.4%, respectively. And the viability of expanded, hatching, and hatched blastocyst of frozen/thawed embryos was : 75.0%, 75.0%, and 50.0%, respectively. The viability of embryos according to biopsy grade of 10∼20%, 21∼30%, and 31∼40% was 85.7%, 91.5%, and 71.4%, respectively. 3. Pregnancy rate after transfer with biopsied embryo between flesh and frozen/thawed embryos was 22.6% and 20.0%, respectively. 4. In comparison between sex by PCR method and sex of calves born after embryo transfer, the accuracy of sex deterimination was 92.3% (12/13).

본 연구는 조경적 측면에서의 모법인 대한민국 헌법의 여러 조문 중에서 환경보전과 생태학이라는 객체에 직, 간접적으로 관련되어 있다고 판단되는 조문들을 자연과학적 관점에서 분석했으묘, 현재 적용되고 있는 환경보전과 생태학과 관련된 국내법(법률: 40종, 지침: 1종, 판례: 1종)과 국외법(헌법: 1종, 법률: 34종, 조례: 2종, 판례: 3종)을 수집, 비교하였다. 또한 수집된 국내, 외 관련볍규를 법조문상의 내용을 기준으로 하여 환경(생태)보전의 기본원칙 및 개념, 계획수립과 사업시행, 유형, 정책 및 관련사업, 관련권리(권한) 및 의무(책임), 허용 및 규제행위, 환경영향평가 및 행정절차, 제도 등의 항목별로 분류하여 비교, 분석해 봄으로써 여러 법률에 산재해 있는 환경보전과 생태학 관련법규를 일목요연하게 조합하여 상호 관련성을 고찰해 보고, 외국의 각국들이 어떻게 환경보전과 생태학 관련 법규정들을 타 분야로부터 독립시켜 왔으며, 환경적인 측면에서 선진화된 사업을 해오고 있는가를 검토해 보았다.