우리나라 전통 음료의 품질 향상과 산업화를 위한 기초 실험으로 오과차의 추출 시간에 따른 성분을 분석하였다. 오과차의 추출 시간에 따른 산도는 30분인 경우 2.4%, 50분에 2.3%, 70분에 1.7%였으며, 고형분은 추출 시간에 따라 각각 0.22g, 0.31g, 0.41g 이었고, pH는 4.70, 4.85, 4.98로 약한 산성을 나타냈으며, 총당은 54.01㎎, 108.82㎎, 142.92㎎으로 추출 시간이 길어짐에 따라 증가하였다. 오과차의 유리당은 glucose, sucrose, fructose, maltose, xylose 5종이 확인되었는데 유리당의 총량은 각각 1.14㎎, 1.36㎎, 2.17㎎ 함유하긴 있었으며 이중 glucose, sucrose, fructose가 전체의 98.2%, 92.6%, 92.6%로 그 대부분을 차지하고 있었다. 오과차의 추출물에서는 30분, 50분 추출시 valino, cystine을 제외한 16종의 아미노산이, 70분 추출시 cystlne을 제외한 17종의 아미노산이 검출되었다. 총유리 아미노산의 함량은 각각 84.94㎎, 99.67㎎, 120.40㎎으로 증가하였으며, serine, glutamic acid, threonine, proline, alanine의 5종 아미노산이 각각 94.5%, 93.6%, 94.7%로 대부분을 차지 하였다. 추출 시간에 따른 유리 아미노산의 함량 비율의 변화는 threonine이 50분간 추출하였을 때 헌저한 감소를 가져왔고, glutamic acid가 추출 시간이 증가함에 따라 점차 감소하였으며, proline은 50분 추출하였을 때 그 비율이 가장 높게 증가하였다.

The vertical distribution and activity patterns of fishes during the evening and morning transitions between day and night were studied acoustically and by bottom trawling in November 1990-1992 in thermally stratified waters of the East China Sea. The acoustic data were collected from six stations with a scientific echo-sounder operating at two frequencies of 25 and 100kHz, and the echograms were used to determine the vertical distributions of fish. Biological sampling was accomplished by bottom trawling to identify fish species recorded on the echograms, and the species and length compositions were determined. At each station, vertical profiles of water temperature, salinity and dissolved oxygen were taken with a CTD system and were related to the diel movements and the depth distributions of fish. During the day most fish were within several meters above bottom, but began to migrate upwards just before sunset, and during the night they were dispersed in midwater. Prior to sunrise with a thermocline present, one group of the fish aggregation occurred in dense schools slightly above the thermocline, while the other group occurred with the numerous single fish-traces bellow it. These groups of aggregations rapidly began to migrate toward the bottom across the thermocline from about 40 min before sunrise. Trawl hauls in the bottom strata below the thermocline with the characteristic single fish traces yieled invariably catches dominated by snailfish and fishing frog with minor quantities of other species in all stations. Hence, the results indicate that snailfish and fishing frog were the dominated scatterers in the depth strata below the thermocline, and the single-fish recordings were mainly snailfish. The fish species such as anchovy and juvenile mackerel in bottom trawl catches is poorly represented in relation to the mesh selectivity of the trawl net, but their occurrence suggest that the fish-school recording above the thermocline were due to these species which migrated vertically across the thermocline, with a temperature gradient of about 8℃, from the water layers near the bottom at night. Accordingly, we conclude that the vertical distribution and activity patterns of snailfish were strongly temperature dependent and in the termally stratified waters, the upper limit to diel activity was closely linked to the position of the thermocline.

The present experirnents on cryopreservation were carried out to investigate effect of solution toxicity, equilibration time and cell stages on the post-thaw survival of mouse morulae and blastocyst embryos cryopreserved by vitrification in EFS solution. The mouse embryos were exposed to the EFS solution in one step at room temperature, kept in the EFS solution during different period for toxicity test, vitrified in liquid nitrogen and thawed rapidly. After the mouse morulae embryos were exposed to EFS solution for 2 and 5 ruin. at room temperature and then they were washed in 0.5 M sucrose solution and basal mediurn(D-PBS + 10% FCS), they were cultured to examined cryoprotectant toxicity induced injury during exposure, most of embryos developed to expanded blastocysts(100 and 90.0%). However, when the exposure time was extended to 10 and 20 min, these development rates dropped dramatically in 10 ruin. (75.0%) and 20 ruin. (4.5%), respectively. When the compacted morulae were vitrified in EFS solution after equilibration for 2 and 5 min, the embryos have developed to normal blastocyst following thawing, washing and culture processes was 89.3 and 89.6%. However, when the exposure time was expanded to 10 ruin, this survival rate dropped to 68.8%. When the blastocyst were vitrified in EFS solution after equilibration for 2, 5 and 10 minutes, the survival rate of embryos which developed to normal blastocyst following thawing and culture processing were 58.5, 46.7 and 22.4%, respectively. The optimal time of equilibration of mouse morula and blastocysts in EFS solution seemed o be 2 and 5 ruin.

In vitro development of parthenogenetic embryo was examined after ethanol treatment of follicular oocytes matured in vitro for 42, 48, 54 and 60h in the pig. The follicular oocytes were matured in TCM 199 containing 15% FCS and gonadotrophins in an atmosphere of 39 5% . The cumulus-free oocytes were activated by 10% ethanol treatment in M2+4mg /ml BSA for 10 min. The ethanol-activated oocytes were washed and further cultured in TCM199+20%FCS containing granulosa cell monolayer. Maturation rates at 42, 48, 54 and 60h of IVM were 75.0, 86.5, 81.6 and 87.9%, respectively. Thus the oocytes maturated in vitro for longer periods did not improve nuclear maturation much. Pronuclear formation rates at 18h post-activation in ethanol-activated oocytes were 21.9, 25.0, 47.4 and 32.6%. The cytoplasmic maturation leading to pronuclear formation upon activation increased when the I VM period was extended from 42 to 54h. When the activated oocytes were cultured for 96~120h to analyse early development of the activated oocytes, the rates of embryonic development upto 5~8 cell were 5.3, 5.8, 12.0 and 11.7% among the cultured embryos. The result indicate that earlier development of activated porcine occyte is dependent on the duration of oocyte maturation, and that better development could be obtained from the oocyte matured for 54h.

The objective of this study was to develop an effective in vitro production system capable of obtaining more porcine embryos from immature oocytes. These experiments were thus conducted to examine the effect of oocytes type and maturation time on the in vitro maturation(IVM) and fertilization(IVF) of oocytes and the in vitro development (IVD)of IVF embryos. 1. The degree of oocyte maturation based on cumulus expansion index(GEI) did not differ for A- and B-typed oocytes but the index of oocyte type C was lower(P<0.05) than that of other oocyte types. 2. When the oocytes of type A and B were matured for 36, 42 and 48hrs, the GEl was not different between the 36- and 42-h maturation but the GEl after 48hrs was greatly lower(P<0.05) than that of other maturation times. 3. The highest cleavage rate(48.6%) of IVF oocytes was obtained from A typed oocytes and 42-h maturation but the developmental potential based on cleavage index was the highest when B-typed oocytes were matured for 42hrs.

The present experiments on cryopreservation were designed to examine the effects of solution toxicity, equilibration time and cell stages on the post-thaw survival of bovine IVF embryos. The oocytes were matured in vitro(IVM) for 24 hrs. in TCM-199 supplemented with 35 g /ml FSH, 10 g /ml LH, 1 g /ml estradiol-17 and granulosa cells at 39 under 5% in air. They were fertilized in vitro(IVF) by epididymal spermatozoa treated with heparin for 24 hrs., and then the zygotes were co-cultured in vitro(IVC) with bovine oviductal epithelial cells for 7 to 9 days. The bovine IVF embryos were exposed to the EFS solution in one step at room temperature, kept in the EFS solution during different period for toxicity test, vitrified in liquid nitrogen, and thawed rapidly. 1. after the bovine blastocysts were exposed to EFS solution for 2 min. at room temperature and then they were washed in 0.5 M sucrose solution and TCM-199, they were cultured to examined cryoprotectant induced injury during exposure, Most of the embryos(95.0%) developed to reexpanded blastocoels. However, when the exposure time was extended to 5 and 10 min, these development rates dropped dramatically in 5 min. (69.5%) and 10 min. (47.4%), respectively, 2. When the bovine IVF embryos were vitrified in EFS solution after the equilibration for 1 and 2 min. exposure, The embryos to have reexpanded blastocoels following thawing, washing and culture processes were found to he 82.6 and 73.9%, respectively. However, when the exposure time was extended to 3 min, this survival rate dropped to 18.2%. The optimal time for equilibration of bovine IVF blastocysts in EFS solution seemed to he 1~2 min. 3. When the bovine IVF embryos were equilibrated for 1 min. the significantly (P<0. 05) higher post-thaw survival rates were obtained from the embryos of blastocyst stage(81.3%) than morulae stage(5. 1%). The optimal cell stage for viterification with EFS solution proven to he blastocyst stage in bovine IVF embryos. 4. The number of blastomeres of blastocyst stage was examined with nuclear staining with Hoechst 33342 during 7 to 9 days post-insemination. The cell counts of frozen bovine IVF embryos were found significantly(P7.5 and those of the fresh embryos 76.67. 1, which were cultured in the sarne period and conditions as frozen embryos.

The DC Servo Motor has been used as an actuator in automatic control fields because of the good response and the control easiness nevertheless it has some disadvantage such as spark at the brush. Recently, along with the fast development of semiconductor industries, the digital control scheme is increasing in comparison with analog control because of the strength against noise and the accuracy. In this paper, authors proposed a combined control algorithm, which is mixed Modified Minimum Time Position Control(MMTPC) and PI control algorithm. for minimum time position control of DC Servo Motor by the one-chip microcontroller. The proposed control algorithm showed the fast response and offset-free. The validity of the proposed method comparing with the VSS control is proved by the response experiments.

수율에 미치는 soyprotein-CaSO_4 응고제의 압착무게와 압착시간의 영향, SPI 두부의 보수력과 조직특성 등을 조사하였다. 두부는 90℃에서 SPI 현탁액에 CaSO_4를 첨가하고 제조하여 압착하였다. 압착 압력이 8.66g/c㎡에서 49.43g/c㎡로 증가함에 따라 부피 수율과 여과지에 흡수되는 수분이 감소하고 견고성, 부착성, 응집성, gum성은 증가하였다. 견고성과 gum성의 증가는 낮은 압력에서 보다는 높은 압력에서 현저하였다.

These studies were conducted to: a) investigate work patterns and productivity indices, b) rate performance levels of employees and c) determine the suggested levels of personnel and labor hours for the effective labor control in school foodservice. Eighteen elementary school foodservices in Seoul were selected in order to analyze work patterns by the work sampling methodology. Allowance time and performance rating by VTR observation was done to determine the standardized labor hours. The results were as follows. The average percentage of each work function of the total work functions such as direct work function, indirect work function and delay were 65.57%, 8.12%, 26.31% respectively. The productivity index is 0.92 min/meal. The average working and delay hours per week of the foodservice director, foodservice employees and supply person were 33.64 hours, 23.25 hours, 38.52 hours respectively. The percentage of delay hours of total labor hours for foodservice employees and supply person were 42.27% and 24.0%. The standardized work hours and the appropriate levels of foodservice employees of 17 elementary school foodservices were examined: The average rating of the foodservice employees work was 1.19 and British Insulated Calendarer Cables (BICC) allowance rate was 19.40% on the average. The total work hours of foodservice employees were 172.64 hours per week and levels of personnel were 4.53 persons. BICC allowance rate was applied: The standardized work hours per week was 180.95 hours and appropriate levels of personnel were 4.11 persons based on legal 44 working hours.