Ionic liquids (ILs) have been used in DNA extraction/separation, DNA preservation and PCR based on their characteristic affinity to DNA. However, few studies have been performed about how DNA-IL complex forms and its mechanism which would be essential to understand the role of ILs over the range of applications. Herein, we present that the differences in the structure of the DNA- IL complex are associated with the alkyl chain length of IL. The assumption was evidenced by Atomic force microscopy, DNA specific dye staining, gel-electrophoresis and real-time electrical measurement. We observed unique electrical signals with altered duration time and amplitude when DNA- ILs complexes pass through solid-state nanopore. We examined three types of ILs (EMIM-Cl, BMIM-Cl, and OMIM-Cl) for their characteristics to form DNA-ILs complexes. The results indicated that the length of hydrophobic alkyl group in respective ILs determines the form of DNA-IL complex. In conclusion, the morphology of DNA could be modified by the incorporation with different alkyl chain length of ILs, providing their further application in biosensor such as nanopore technique for DNA sequencing or understanding protein-DNA interaction.
이 연구는 법무부에서 시행하는 직업훈련 과정에 참여하는 수형자들을 대상으로 학습전이에 영향을 주는 요인이 무엇이며, 각각의 요인이 이에 미치는 직접적인 효과뿐만 아니라, 전이 요인간의 관계를 규명하고자 하였다. 그리하여 실제 교정시설에 직업훈련교육을 받고 있는 수형자를 연구대상으로 선정하고 설문조사를 실시한 결과는 다음과 같다. 첫째, 기술통계 분석결과 5점 척도의 중간 값인 3을 넘어서 수형자 직업훈련의 성과를 이루고 있다고 할 수 있다. 그리고 모든 변인에서 측정된 결과 값이 중간 값 이상으로서 수형자 직업훈련의 타당성과 책무성도 이루고 있다고 볼 수 있다. 둘째, 조직적 지원과 교육만족도가 중요한 변인으로 밝혀졌고, 본인들의 학습동기나 만족도와 같은 내생변인을 통하여 효과를 발생하는 것으로도 나타났다. 즉, 수형자들이 출소 후 배운 내용을 현업에 적용하기 위해서는 기관의 지원이 크게 작용하는 것으로 나타났다. 마지막으로 전이설계가 실제 현업과 유사한 학습 환경을 구성하여 훈련에서 습득한 내용 등을 활용하게 하고 전이를 촉진한다고 할 수 있다. 따라서 전이설계가 높을 때 학습전이가 잘 나타나는 것으로 결론지을 수 있다.
This paper dealt with the applicability of GFRP materials as reinforcements for a steel box girder bridge deck. The purpose of this study is to provide detailed design procedures with a code-based text for GFRP composites for civil engineering structures. From the example design, the deck was optimized from a serviceability perspective but was quite overdesigned with regard to flexural strength and creep rupture stress, due to its relatively low longitudinal modulus but high strength. We may conclude from these results that it is advisable to check the serviceability limits before optimizing the design for strength or starting the design from the serviceability calculation.
Bridge inspection structures are the structure which is installed on the piers, abutments, and copings for the inspection and maintenance of substructure. In this study, the structural performance of the bridge inspection structures using aluminum members manufactured by extrusion process is evaluated. The bridge inspection structures can be installed regardless of the shape of concrete surface through the simple cutting process. The structural performance of bridge inspection structures is evaluated using FE analysis. Moreover, experimental studies are conducted for the estimation of the structural safety of the members for the design load.
The proposed hybrid damper installs at a coupling beam and consists of a high-damping rubber (HDR) and steel pin. The proposed hybrid damper adopted a pin-lock system acts as a viscoelastic damper under wind load (small displacement) while it behaves as a hysteretic damper under earthquake load (large displacement).
In this paper, the pin-lock mechanism and structural performance of the proposed hybrid damper is evaluated through experiment. Experiments were carried out with the variables which displacement, loading frequency and steel pin quantities were used. Test results showed that the pin-lock mechanism and the performance of the hybrid damper under a large displacement were verified. Also equivalent damping ratios of HDR were increasing at a small displacement as displacement amplitudes were increasing. However HDR did not depend on frequency,
Determining of the shape in the process of design for natural draught cooling tower is very important, because the shape of hyperbolic shell is respond sensitively to dynamic behavior of the whole cooling tower against wind load. In engineering practice, the geometric parameters have been determining based on the natural frequency. This study analyses influence of the tower shell geometric parameters on the structural behavior. For three representative models were selected, they were analyzed based on evaluation of damage by means of nonlinear FE-method. As a result, a hyperbolic rotational shell with the small radius overall was the lowest damage index induced by sufficient capacity of the stress redistribution and thus a wind-insensitive structure.
Shelter that communication equipment and on-equipment material are mounted on is transported by airplane or vehicle, it has a function such as waterproof and shielding EMI. When the shelter is transported by car or helicopter, it is shocked by vibration and crash with the ground. So, three skids are attached to bottom of the shelter to reduce the shock. To confirm the durability of the structure of shelter, parallel drop and rotating drop test were done in accordance with KDS 0000-0000. However, the damage was discovered in the shelter. So, stiffening plate was added to skid and panel of partition wall to obtain durability and changed the shape of rubber buffer. In this paper, the cause of deformation and damage in the shelter was analyzed and improved shape through the structural analysis was verified.
The characteristics of stress distribution and fatigue life have been investigated in the road mower head system using FEM(Finite Element Method). There is high stress distribution around the coupling part with non-uniform load from the irregular road surface. Maximum equivalent stress especially occurs in the fixed plate and the pillar of the head device with maximum deformation at the rotation axis frame of the mower system. Fatigue life tests were also performed with SAE bracket history, SAE transmission, and sample history conditions. SAE bracket history and SAE transmission predicted the possibility of high damage while Sample history showed a stable trend. These results will be applicable in optimal design for various systems equipped with mower head system.
CFRP hardened by carbon fiber and resin has the property of high strength and low weight. Specifically, the strong feature against the external vibration environment is shown as CFRP is designed with the structure of multi-axes. So, CFRP in place of metal has been used at the various fields. CFRP specimens for mode Ⅱ are applied with the repetitive fatigue load in this study. These specimens have the fiber layer angles of 30°, 45° and 60°. The material properties of specimens are investigated with the result of fatigue fracture due to this load. As the study result, the smallest and largest reaction forces of 500 N and 540 N are shown at the layer angle of 30° and 60° respectively among these specimens. The separation of adhesive interface at 4000 fatigue cycles is happened earliest in case of the layer angle of 60°. But the separation of adhesive interface at 11000 fatigue cycles is happened latest in case of the layer angle of 45°. Through the result of fatigue property, it is thought that the basis data can be applied to evaluate the safety at CFRP structure applied with fatigue.
Geum’yeok-dang house in Andong was originally constructed in 1558. The original floor plan of the upper base featured a 90-degree tilted ‘日’ shape, and had inner court(內庭) on each side of ‘Jungdang(中堂, center hall)’ building that was placed on the south-north axis. When designing the building, the Neo-Confucian client of Geum’yeok-dang applied his understanding of how Ga’rye(家禮) defined the structure of ritual space. Consequently, ‘Daecheong(大廳)’, the place where guests were greeted and ancestral rites and coming of age ceremonies for male were held, was built in a protruding fashion. ‘Jungdang’[otherwise known as ‘Jeongchim(正寢)’], where coming of age ceremonies for females were held, the master of the house faced death, and memorials for close ancestors were held, was placed at the center of the residence on the south-north axis. The Geum’yeok-dang today was greatly renovated in the early and mid 18th century, due to the spread of ‘Ondol’ heating system. As the Ondol heating system became popular, the pre-existing drawbacks and the inconvenience of the house stood out clearly. As a result, the house was renovated into today’s structure consisting of ‘口’ shape ‘Anchae’ and ‘Daecheongchae’ in separate building.
Finite Element analysis were carried out to investigate the deformation behaviours of a buckled automotive seat frames made of three different types of materials, i.e., SAPH440, Al6082-T6 and Al7021-T7, when they were subject to external load, based on the ECE R14 regulation to achieve lightweight structure. Also, several thicknesses were applied to the seat frame structures of each material for characterising deformations. It was found that light weight seat frame structure was obtained compared to conventional steel structure when it was made of aluminium under the condition of satisfying ECE R14 regulation. Interpretation result, when changing from SAPHH440 material has a thickness of 1.5mm to Al material has a thickness of 3.0mm, that could checking weight lightening about 47%.
Modular buildings are constructed by assembling modular units which are prefabricated in a factory and delivered to the site. However, due to a problem of noise between floors, concrete slab is usually poured at the top or bottom level of a modular unit in Korea. This greatly increases the weight of buildings, but designing vertical members of modular units to resist overall gravity loads is very inefficient. In this study, considering domestic building construction practices, feasible structural systems for tall modular buildings are proposed in which separate steel frames and reinforced concrete core walls are designed to resist gravity and lateral loads. To verify performance, a three-dimensional structural analysis has been performed with two types of prototype buildings, i.e., a residential building and a hotel. From the results, wind-induced lateral displacements and seismic story drifts are examined and compared with their limit values. Between the two kinds of buildings, the efficiency of the proposed system is also evaluated through a comparison of the weight of structural components. Finally, the effect of a floor diaphragm on the overall behavior is analyzed and discussed.
This study deals with optimized structural analysis of stainless rectangular water reservoirs with 5,000ton capacity for various combined load cases. The objective of this study is to propose most efficient structural models through the comparison of various model cases. In order to perform an optimized analysis, three dimensional finite element analyses are carried out for large sized models. The numerical results obtained provides the detailed size and thickness for optimal design of water reservoir. In particular, results reported in this paper show the influence of various types of loading and dimensions of the wall and stiffened column on the structural behavior of the large sized water tanks.
This study aims to estimate the species, size and shape of fish using a non-contact 3 dimensional pattern laser so that this preliminary test was carried out to understand the structural feature and length of goldfish according to water turbidity and depth in the aquacultural tank. 3-D pattern laser could clearly detect its morphological shape except the caudal fin due to soft tissue. Since the sensing strength of line laser light according to depth has sufficient power, it is possible to measure its depth and structural feature in the detected range. The result showed that the measured error of individual’s fork length was less than ±1% in the water using 3-D pattern laser, when compared with the measured value in the air.
This study is about the basic design technology to radically increase the structural stability of structural shell or tube, which are utilized in a variety of large structures like aircrafts, plant, bridges and buildings. Recent studies have revealed that the plates stiffened by closed-sections ribs can be designed to have greater strength as well as the reduction of used number of stiffeners. Then, the analytical models were selected based on the huge steel tube design and the finite element modeling has been conducted using the ABAQUS. Through this study, the elastic buckling strengths are compared with the flat plate buckling stress and the improved effect in the local buckling strength due to the closed-section ribs are numerically verified.
In this study the structural performance of new composite column to composite beam connection was evaluated by cyclic tests. It is designed to be applied to various types of through type system to the bottom plate to view synthesis composite beam. A composite column is composed of a RC type column reinforced by steel angles which the end bends in each edge corners of column. also, A composite beam is composed of trussed type’s web, a bottom steel plate and reinforced by steel angles which the end bends in each edge corners of beam. 4 cyclic tests of the clarified structural performance of the connections are performed. All specimens that is apply reinforced concrete beam-column joints based on KBC2009 criteria was performed by cyclic test of column to beam. All specimens were exposed to be satisfied with the required ultimate strength.
Generally, antiseptic synthetic woods are used in septic environment because of their durability and relatively fine view. However, they have problems such as a failure of connection between the members under the septic conditions. In this study we investigate a fiber reinforced polyurethane foothold to be used in the wet environment to replace the synthetic wood. The fiber reinforced polyurethane foothold is consist of 2 different layer of materials. First layer is a glass fiber reinforced polymeric plastics (GFRP) which is located at outer surface of the foothold. This GFRP outer layers carry most parts of load. Second layer is a polyurethane layer which transfers the load to each outer layer. Flexural tests for this foothold are conducted. From the flexural tests it is confirmed that the structural performance of foothold test specimen with different number of GFRP layers and density of the polyurethane.