Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria’s early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

To investigate the effect of thinning intensity on environmental factors and ectomycorrhizal mushroom fruiting in forest ecosystems, we studied canopy closure, throughfall, soil temperature, soil moisture, light response of understory vegetation, and ectomycorrhizal mushroom fruiting in a 10-year-old pine forest after 34%, 45%, and 60% thinning. Canopy closure was significantly higher in the 34% treatment and control plots, ranging from 80–85% in April. However, in November, all thinning treatment plots showed a decrease of approximately 5–10% compared with the control plot. The 60% treatment plot had over 200 mm of additional throughfall compared with the control plot, and monthly throughfall was significantly higher by more than 100 mm in October. The soil temperature in each treatment plot increased significantly by up to 1°C or more compared with the control plot as the thinning rate increased. The soil moisture increased by more than 5% in the thinning treatment plots during rainfall, particularly in the 34% treatment plot, where the rate of moisture decrease was slower. The photosynthetic rate of major tree species (excluding Pinus densiflora)was highest in Quercus mongolica, with a rate of 7 μmolCO2·m-2·s-1. At a lightintensity of 800 μmol·m-2·s-1, Q. mongolica showed the highest photosynthetic level of 6 ± 0.3 μmolCO2·m-2·s-1 in the 45% treatment. The photosynthetic rate of Fraxinus sieboldiana and Styrax japonicus increased as the thinning intensity increased. The Shannon– Wiener index of mycorrhizal mushrooms did not significantly differ among treatments, but the fresh weight of mushrooms was approximately 360–840 g higher in the 34% and 45% treatments than in the control. Additionally, the fresh weight of fungi in the 60% treatment was 860 g less than that in the control. There were more individuals of Amanita citrina in the control than in the thinning treatment, while Suillus bovinus numbers increased by more than 10 times in the 34% thinning treatment compared with the control.

Targeting Microcystin (MC), which is most abundantly detected in the North-Han River water area, we analyzed the relationship between the MC biosynthesis gene (mcyA gene), cyanobacteria cell density, and MC concentration, derived an RNA-MC conversion formula, and derived the cyanobacteria. The concentration of MC present in cells was predicted. In the North-Han River waters, the mcyA gene was found mainly at downstream sites of the North-Han River after Muk-Hyeon Stream junction, and higher copy numbers were found on average than other sites. In the Uiam Lake waters upstream of the North-Han River, the mcyA gene copy number increased at the Kong-Ji Stream point, and after September, the mcyA gene copy number decreased throughout the North-Han River waters. The expression of the mcyA gene was concentrated in the short period of summer due to the spatio-temporal difference between upstream and downstream water bodies. The mcyA gene expression level was not only highly correlated with MC concentration, but also correlated with the cell density of Microcystis aeruginosa and Dolichospermum circinale, which are known to biosynthesize MC. Six conversion formulas derived based on the RNA-MC relationship showed statistical significance (p<0.05) and exhibited high correlation coefficients (r) of 0.9 or higher. The expression level of MC biosynthesis gene present in eRNA determines the synthesis of cyanotoxin substances in water, quickly quantifies gene activity, and can be fully utilized for early warning of MC development.

The interest in greenhouse gases (GHG) emitted from all industries is emerging as a very important issue worldwide. This is affecting not only the global warming, but also the environmentally friendly competitiveness of the industry. The fisheries sector is increasingly interested in greenhouse gas emissions also due to the Paris Climate Agreement in 2015. Korean industry and government are also making a number of effort to reduce greenhouse gas emissions so far, but the effort to reduce GHG in the fishery sector is insufficient compared to other fields. Especially, the investigation on the GHG emissions from Korean fisheries did not carry out extensively. The studies on GHG emissions from Korean fishery are most likely dealt with the GHG emissions by fishery classification so far. However, the forthcoming research related to GHG emissions from fisheries is needed to evaluate the GHG emission level by species to prepare the adoption of Environmental labels and declarations (ISO 14020). The purpose of this research is to investigate which degree of GHG emitted to produce the species (swimming crab and snow crab) from various fisheries. Here, we calculated the GHG emission to produce the species from the fisheries using the life cycle assessment (LCA) method. The system boundary and input parameters for each process level are defined for LCA analysis. The fuel use coefficients of the fisheries for the species are also calculated according to the fuel type. The GHG emissions from sea activities by the fisheries will be dealt with. Furthermore, the GHG emissions for producing the unit weight species and annual production are calculated by fishery classification. The results will be helpful to establish the carbon footprint of seafood in Korea.

PURPOSES : With the recent enactment of the 「Framework Act on Sustainable Infrastructure Management」 in Korea, the establishment of mid- to long-term management plans for social infrastructure and the feasibility evaluation of maintenance projects have become mandatory. To this end, the life cycle cost analysis is essential. However, owing to the absence of a deterioration model, trials and errors are in progress. METHODS : In this study, a deterioration model was established for bridges, which are the representative social infrastructures of roads, particularly for expansion joints that can cause enormous damage to not only the superstructure but also the substructure. The deterioration model was classified into rubber and steel, based on the material of the expansion joint. The analysis used the inspection and climate data conducted in Korea over the last 12 years. The Bayesian Markov Hazard model was applied as the analysis technique. RESULTS : The average life expectancy by type of expansion joint was analyzed to be 8.9 and 6.6 years for rubber and steel, respectively. For probabilistic life cycle cost analysis, the probability distribution of the life expectancy, validity range by confidence level, and Markov transition probability matrix were presented. CONCLUSIONS : In this study, the basis for deterministic and probabilistic life cycle cost analysis of expansion joints was laid. In future studies, it will be necessary to establish a standardized deterioration model for all types of infrastructure, including all bridge elements.

This study is to develop a radish skin peeling machine. Currently, radish peeling work is carried out manually, so the unit price is high due to the increase in labor cost, and problems related to hygiene continue to occur. To solve this problem, a field supply type radish skin peeling machine was designed and manufactured. The peeling machine is a principle in which the skin is removed by conveying the radish using a roller and passing through a blade assembly. In this paper, the design and analysis of the roller and blade part applied during the movement of the radish were carried out, and structurally stable results were obtained.

Sampling gears for collecting fish are diverse, and the community of fish varies according to the selection and characteristics of the sampling gears. The present study compared the characteristics of fish communities in Yedang reservoir using four sampling gears (kick net, cast net, gill net, and fyke net). The kick net and cast net were inefficient in collecting the number of individuals. However, they increased the species diversity of fish inhabiting the waterfront. Although not many individuals were collected, the gill net mainly collected large fish. The largest number of individuals was collected in the fyke net, and the dominance was high due to the high species selectivity. Through Self-Organizing Map (SOM) analysis, large fish were collected in the gill net, whereas small fish were collected in the fyke net. The characteristics and efficiency of the fish differed depending on the sampling gears. It is expected that researchers will need to use it appropriately according to the characteristics of the sampling gears when investigating the fish community.

도서관은 문화 공간으로서 누구나 이용할 수 있고 다양한 분야의 지식과 정보를 제공하여 삶의 질을 높이는 중요한 사회기반 시설 중 하나이다. 현재 한국의 도서관 수는 수요에 비해 공급이 부족한 상황이며, 이를 해결하기 위해 일부 지자체는 차량을 수단으로 이동형 도서 서비스를 제공하는 분관 형태의 이동 도서관을 운영하고 있다. 주로 도서관을 이용하기 어려운 사람들을 대상으로 순회하며 도서관 서비스를 제공하지만, 비효율적인 운행 노선과 균일하지 않은 서비스로 실질적인 효과를 발휘하지 못하고 있다. 따라서 본 연구에서는 성남시 새마을 이동 도서관을 대상으로 순회 경로 현황을 파악하고, 최소 이동 거리로 개선된 이동 도서관 노선을 제시하고자 한다. 더욱 효율적인 운영을 위해 서비스 권역을 나누고, 시간 제약을 결합한 차량경로설정 문제를 사용하여 도서 서비스의 이용 격차를 줄인 새로운 운행 노선을 구축하였다. 본 연구는 이동 도서관의 효과적인 노선 운영에 대한 기초적인 자료로 활용될 수 있다는 점에서 의의가 있다. 향후 이동 도서관 뿐만 아니라 이동형 공공 서비스를 위한 유용하고 현실적인 가이드라인으로 활용될 수 있을 것이다.

국내에서 공용중인 교량은 33,177개로 사회기반시설 중 가장 많은 비중을 차지한다. 이러한 교량은 공용하중, 온도, 습도 등에 의해 거더에 신축량이 발생하게 되고, 거더간 유간거리에 대한 안정성을 확보하기 위해 신축이음장치를 설치한다. 신 축이음장치에는 교량의 누수 및 퇴적물 낙하 등을 방지하기 위해 고무지수재를 설치하는 것이 일반적이다. 하지만 이러한 고무 지수재는 다양한 원인에 의해 쉽게 손상이 발생하게 된다. 손상된 고무지수재를 통해 거더의 부식, 교량하부 인명사고 등 다양 한 2차 피해가 발생할 수 있다. 이러한 피해를 예방하기 위해 교량의 유지관리를 수행하고 있지만 고무지수재 특성상 지속적인 교체는 불가피한 실정이다. 따라서 본 연구에서는 기존 신축이음장치에 활용되는 고무지수재의 문제점을 해결하기 위하여 초탄 성 형상기억합금을 활용한 새로운 지수재 개발 연구를 수행하였다. 초탄성 형상기억합금 지수재에 대한 유한요소해석을 수행 및 분석하였으며, 복원 효과를 통해 지속 사용 가능한 지수재 연구를 검증하였다.

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), a chronic contagious disease in small ruminants. The prevalence of CLA has been reported to be >50% in Korean black goats. CLA is difficult to control due to a lack of efficient vaccines and treatment methods. Effective disinfection of the farm environment may be an alternative strategy for reducing the spread of C. pseudotuberculosis. The objective of this study was to evaluate the efficacy of commercial disinfectants against CLA. The six commercial disinfectants, largely composed of sodium dichloroisocyanurate, sodium hypochlorite, potassium monopersulfate triple salt, quaternary ammonium, citric acid, and copper sulfate, were tested against five different genotypes of C. pseudotuberculosis isolated from goat farms in Korea. Efficacy tests were performed in accordance with the disinfectant efficacy test guidelines recommended by the Animal and Plant Quarantine Agency of Korea with slight modifications. All disinfectants except for copper sulfate exhibited >99.99% killing efficacy under hard water conditions following 30 min of incubation, which is the recommended standard treatment time according to guidelines. The minimum bactericidal treatment time was evaluated by employing treatments for durations of 1, 5, and 15 min. The most effective compounds under hard water conditions were sodium dichloroisocyanurate, potassium monopersulfate triple salt, and sodium hypochlorite, exhibiting >99.99% killing efficacy after 1 min of treatment. In the aqueous solution forms, citric acid and the quaternary ammonium compound were the most effective, but required at least 5 min to kill >99.99% of the bacteria. The current study characterizes the killing efficacy of six commercial disinfectant active compounds against C. pseudotuberculosis. Thus, this study provides essential information regarding the efficacy of the disinfectants used to control CLA in goat farms.

본 연구에서는 식품접객업소에서 시중 판매되는 더치커 피의 미생물 오염도를 조사하여 유형에 따른 차이를 살펴 보고자 하였다. 경기도 내 커피전문점에서 유통되는 더치 커피 70건을 대상으로 위생지표균, 식중독균, 진균류 오염도를 조사하였으며 추출방법에 따른 일반세균과 진균류의 오 염도를 비교하였다. 일반세균수는 0.74-6.21 log CFU/mL 범 위로 검출되었으며 식품 유형에 따른 평균 검출량은 식품 접객업소 조리식품(3.04 log CFU/mL)이 액상커피(2.08 log CFU/mL)보다 높게 나타났다. 액상커피는 검출된 6건 중 3건이 세균수 기준을 초과하여 부적합이었다. 식중독 원 인균은 70건 모두 불검출이었다. 진균수는 0.70-4.00 log CFU/mL 범위로 검출되었으며 식품유형에 따른 차이는 없 었다. 추출방법에 따른 미생물 오염도는 원두 혼합 여부 와 추출 시간에 따라 비교하였으며, 일반세균수와 진균수 모두 유의적인 차이는 없었다(P>0.05). 시중 유통되는 더 치커피는 일반세균과 진균류에 대한 오염도가 높으므로 위생적인 관리와 적절한 온도 유지를 통해 미생물 오염을 줄이는 것이 중요하며 안전성 확보를 위해 지속적인 모니 터링이 필요할 것으로 생각된다.

The purpose of this study is to investigate the status of admission and employment in fisheries high schools (FHS) and to consider ways to revitalize FHS through substantialization. The recruitment rate of new students in FHS decreased from 97.4% in 2016 to 83.2% in 2020. The aging training ship that FHS needs to improve most urgently is being jointly used by FHS across the country, and the construction of a joint training ship managed by the Korea Institute of Maritime and Fisheries Technology is being promoted. The average employment rate for FHS by year was 40.2-59.4%, and the fisheries-related employment rate was low at 31.0-38.9%. On the other hand, the acquisition rate of certificate of competence was 37.5-52.0%, and the rate of employment on board of those who obtained the certificate of competence was 42.9-59.8%. In order to secure new students and improve the recruitment rate, we operate experiential classrooms that reflect the characteristics of training ships and departments and conduct public relations activities using sns, publicity video ucc, YouTube, etc. It will be necessary to expand opportunities for fisheries-related vocational experience through active career exploration and elective courses in the FHS credit system. Finally, it is judged that fisheries related government agencies, industries and local governments need to improve their awareness of FHS and plan to support fisheries manpower nurturing in order to attempt the vitalization of FHS.