무농약 시설재배 오이에서 잎, 꽃 및 과실을 가해하던 응애를 채집하여 동정한 결과 오이긴털가루응애(Tyrophagus neiswanderi)로 동정되었 다. 오이긴털가루응애는 분류학상 응애목(Acarina) 가루응애과(Astigmata)에 속하며 형태적으로는 유백색의 계란형을 띄고 체장은 490.1㎛, 체 폭은 288.1㎛의 매우 작은 응애이다. 오이긴털가루응애는 잎, 꽃, 과실에 모두 발생하여 피해를 주었다. 피해증상은 정식 초기 유묘의 경우 신초가 수축되면서 연노란색으로 탈색이 되고, 심하면 신초가 멎는 피해가 나타난다. 또한 오이가 생육을 하는 과정에서 잎이 피해를 받으면 처음에는 오이 잎에 흰색의 반점이 나타나고 시간이 경과함에 따라 반점에 구멍이 나고 찢어진다. 꽃에서도 흰색 반점과 찢어지는 증상이 나타난다. 과실에서는 어린 과실일 때 피해를 받으면 과실의 비대가 저해되거나 기형과가 나타나며, 과실이 비대하더라도 코르크증상으로 인해 상품성이 떨어지게 된다.

본 연구는 거베라 ‘Harmony’, ‘Sunshine’, ‘Pink melody’를 대상으로 화경 기부의 절단 정도와 수확 후 물올림 시기에 따른 수분 흡수 및 생체중 변화가 절화수명에 미치는 영향을 알아보자 수행되었다. 실험 결과 3 품종 모두 수분흡수, 생체중, 절화수명 및 수분 이동에서 비슷한 경향을 보였다. 화경 기부를 각각 3cm, 5cm, 10cm절단한 절화는 무절단과 절단 1cm 처리구에 비해서 일일수분흡수량은 더 많았고, 최초 생체중 대비 생체중 감소율은 더 낮았으며, 이로 인해 절화수명은 2-5일 연장되었다. 화경 기부의 절단 유무에 따른 수분흡수량 차이의 원인을 구명하기 위하여 현미경 검경을 통해 수분의이동 경로를 관찰한 결과, 화경 기부를 3cm 절단한 경우에는 목부 뿐만 아니라 목부섬유, 사부섬유, 수조직의 주변 세포 등을 통해 수분이 이동하는 반면, 화경 기부를 절단하지 않은 경우에는 목부로만 수분이 이동하는 것을 확인할 수 있었다. 또한 수확 후 물올림 시작 시기의 지연이 거베라의 절화수명에 미치는 영향을 조사한 결과, 화경 기부를 절단하지 않고 물올림 시작 시기를 지연한 절화는 지연된 시간이 길어질수록 화경굽음 및 꽃잎위조 현상이 점차 증가하였으며 절화수명도 짧아진 반면, 화경 기부를 절단한 경우에는 수확 후 12시간 까지물올림을 지연 했음에도 불구하고 절화수명에는 유의한차이를 보이지 않았다. 이는 물에 침지한 후 물관을 포함한 다양한 유관속 조직을 통하여 흡수된 많은 양의 수분이 화경과 꽃잎세포의 팽압을 회복하는데 조력했기 때문인 것으로 판단된다.

The true bug genus Paromius from Korea is revised taxonomically. It comprises the following three species: P. exiguus (Distant, 1883), P. jejunus (Distant, 1883) and P. gracilis (Rambur, 1839). Among them, P. jejunus (Distant, 1883) is recorded for the first time in Korea. The pest bug injurious to rice culture, known as P. exiguus (Distant,1883), has been recorded erroneously by the previous authors from Korea due to the misidentification, which is rectified here with the support of morphological evidence. All the species of this genus are classified with proper redescriptions, and an identification key to species is provided with illustrations.

Thermal death kinetics was examined to develop a heat treatment method to control the Pine wood nematode, Bursaphelenchus xylophilus, in wood packing materials for export goods. To determine the feasibility of microwave irradiation as an alternative treatment, we performed laboratory experiments on the blocks (200x200x250mm) of Douglas-fir artificially infested with live adults with subsequent 2.45GHz microwave energy irradiation. The 100% mortality of treated Pine wood nematode was achieved above 64℃ of the measured wood temperature, regardless of irradiated time. All measurement points in the blocks reached 64℃ within 84-312 seconds with microwave irradiation by wood weight. Likewise, the 100% mortality of nematodes were observed by hot water treatment on 62℃. We need further experimental verification, but commercial microwave (2.45GHz) treatment is a feasible alternative to conventional heat treatment to control Pine wood nematode on the wood packing materials for export goods.

절화용 스탠다드 국화 ‘백설’의 전처리제 효과에 대한 연구에서1-MCP 0.5g·m-3 처리구가 대조구에 비해 2일 정도 꽃봉오리의꽃잎착색과 조기개화가 지연되었으며 개화 정도도 9일 까지 개화를지연시켜 품질선도를 유지시킬 수 있었다. Promalin(GA4+7+BA)100mg·L-1과 500mg·L-1 처리구에서도 개화지연 효과가 나타났으나,500mg·L-1 이상의 고농도에서는 잎과 꽃의 조기 위조현상을 나타내었다. 또한 생체중의 변화에서도 1-MCP 0.5g·m-3, 1.0g·m-3 및Promalin(GA4+7+BA) 100mg·L-1 처리구에서 대조구에 비해 현저히 증가되었다. 이상의 결과로 고찰해보면 1-MCP 0.5g·m-3와Promalin(GA4+7+BA) 100mg·L-1 처리는 절화 국화 ‘백설’의 수확 후 꽃봉오리 화색변화 및 조기개화를 억제시켜 품질 선도유지에매우 효과적임을 알 수 있었다.

Thermal death kinetics was examined to develop a heat treatment method to control the Alnus ambrosia beetle, Xylosandrus germanus, in wood packing materials for export goods. To determine the feasibility of microwave irradiation as an alternative treatment, we performed laboratory experiments on the blocks (200x200x250mm) of Douglas-fir artificially infested with live adults with subsequent 2.45GHz microwave energy irradiation. The 100% mortality of treated Alnus Ambrosia Beetle was achieved above 58℃ of the measured wood temperature, regardless of irradiated time. All measurement points in the blocks reached 58℃ within 84-312 seconds with microwave irradiation by wood weight. Likewise, the 100% mortality of breetles were observed by hot water treatment on 52℃. We need further experimental verification, but commercial microwave (2.45GHz) treatment is a feasible alternative to conventional heat treatment to control Alnus ambrosia beetle on the wood packing materials for export goods.

광원에 따른 국화의 화아분화 억제 효과를 보기 위 하여 형광등과 LED로 암중단 장일처리를 하였다. 청색 LED광원에 의한 광중단 처리시 ‘백마’는 평균초장 이 약 20 cm 정도 신장되고, 평균 생육일수가 약 20.1일 정도 되었을 때 조기에 발뢰되어 화아분화가 억제되지 않았다. 광원에 따른 개화소요일수는 형광등 55.5-61.0일에 비하여 혼합 LED처리에서는 약 0.7-4.6 일, 적색 LED처리에서는 약 5.6-9.3일 개화소요일수가 감소하였다. 따라서 화아분화억제 효과는 형광등과 혼 합 LED처리가 가장 크며, 적색 LED처리에 의해서도 어느정도 화아분화 억제 효과가 있는 것으로 나타났으 나, 청색 LED처리는 오히려 조기발뢰가 되는 것을 알 수 있었다. 형광등과 혼합 LED처리에 따른 절화장은 두 광원에서 우수하였으며, 통계적으로 비슷한 결과를 보였다. 절화중은 품종에 따라 다르게 나타났으며 ‘백 마’와 ‘핑크프라이드’는 형광등처리에 비하여 혼합 LED처리시 가장 무거웠으며, ‘신마’는 형광등에 비하여 가벼웠고, ‘일월’은 같은 경향을 보였다. 꽃목길이는 스 탠다드와 스프레이 국화 모두 적색 LED처리시 가장 짧았으며, 오히려 스프레이국화에서는 청색 LED처리 에서 긴 것으로 조사되었으며, 특히 ‘핑크프라이드’는 10.0 cm로 매우 길었다. 화아분화억제를 위해 36일간 4시간씩 심야에 조명한 전력소모량은 형광등 792.0 kw 에 비하여 혼합 LED에서는 234.7 kw로 70.4%가 절 감되었다.

Papilio xuthus is known as the Asian swallowtail, Chinese yellow swallowtail, or as the Xuthus swallowtail. It overwinters in pupae stage. The study was conducted to investigate the temperature effect on adult emergence of overwintering P. xuthus pupae at four different constant temperatures (15, 20, 25 and 30°C) with photoperiod 10:14(L:D). The lower developmental thresholds of female, male and both sexes combined were 9.71, 11.03, and 10.49°C, respectively. Developmental period of overwintering pupae to adults decreased with increasing temperature between 15 and 30°C. Degree-days for adult emergence to female, male and both sexes combined were 227.27, 175.44, and 200.80DD, respectively. The relationship between the developmental rate and temperature was fitted by linear and five nonlinear developmental rate models (Logan 6, Lactin 1, 2 and Briere 1, 2). The variation of overwintering pupae development was described by the two-parameter Weibull distribution model. The adult emergence of overwintering P. xuthus pupae in spring can be explained and predicted by using developmental rate models and developmental distribution model. The effect of climate change on phenology and distribution of P. xuthus may be presented by the model.

Papilio macilentus is the longest tail butterfly of Papilionidae found in Japan, China and Korea and has distinctive morphological differences between male and female. P .macilentus feeds on Zanthoxylum schinifolium and has three generations per year. The lower developmental threshold and thermal constant for adult emergence of P. macilentus overwintering pupae were estimated at four different constant temperatures (15, 20, 25 and 30°C) with photoperiod 10:14 (L:D). The lower developmental threshold of both sexes combined was 7.95°C. The development of overwintering pupae decreased with increasing temperature between 15 and 30°C. Thermal constant of both sexes combined was 251.26DD. The relationship between the developmental rate and temperature was fitted by linear and five nonlinear developmental rate models (Logan 6, Lactin 1, 2 and Briere 1, 2). The development of overwintering P. macilentus pupae was described by the two-parameter Weibull distribution model. The temperature-dependent adult emergence model of P. macilentus developed in this study could be used to predict the full-cycle phenology model of P. macilentus and applied to understand the effects of climate change on the emergence distribution of overwintering pupae.

Damages by Tyrophagus spp. (Acari: Acaridae) on horticultural crops were newly observed in recent years especially in organic culture greenhouses in Korea. The crops damaged by the mite species were Spinacia oleracea L. (spinach), Cucumis sativus (cucumber), and Phalaenopsis schilleriana (orchid). The spinach inner leaf growth was halted affected by the feeding of Tyrophagus similis at the early stage of the shoot emergence. The young leaves of the spinach bud had small holes and the leaves were deformed as they grew up. The surface of young cucumber fruits became shiny and soft at the early damage stage and the damaged surfaces became corky as the plants grew up. The mite from cucumber was identified as Tyrophagus neiswanderi Johnston & Bruce. In phalaenopsis orchid, the flower buds became yellow and dropped and the lower side of the leaves had white spots. The damages were caused by Tyrophagus putrescentiae.

Three species of the genus Neolygus Knight - N. hakusanensis (Yasunaga, 1991), N. roseus (Yasunaga, 1991) and N. zhugei (Yasunaga, 1991) - are recognized for the first time in Korea. An identification key to the eleven Korean Neolygus species is presented. Some illustrations of male genitalic structures are also provided.

A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.