Ochratoxin A (OTA) is one of the most important mycotoxins due to its prominent nephrotoxicity as well as potential carcinogenicity to human. OTA has been detected in a wide range of agricultural commodities including cereal grains and their processed products. OTA is stable under most food processing conditions while the reduction of OTA is more rapid and extensive under higher temperature and alkaline conditions. In this study, the effects of extrusion cooking on the stability of OTA in spiked (100 μg/kg) rice flour (moisture content, 16% wet weight basis) in the presence and absence of baking soda was investigated using a laboratory scale twin-screw extruder. The extrusion variables were temperature (120 and 150°C), screw speed (150, 200, and 250 rpm), and baking soda (0, 0.5, and 1%, w/w). Both unextruded and extruded samples were analyzed for OTA by high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). The reduction of OTA was in the range of 78-82% with no added baking soda, whereas lesser reductions of 75-80% and 72-77% were observed at 0.5% and 1% baking soda, respectively. Both temperature and screw speed did not affect reduction of OTA during extrusion regardless of the presence or absence of baking soda. While the total color difference (ΔE) of extruded samples were increased with baking soda content, the radial expansion of extruded samples were decreased with baking soda content. These results suggest that OTA in rice flour may be reduced significantly by extrusion. In addition, added baking soda may negatively impact the reduction of OTA while its mechanism of action is unknown.

The tobacco cutworm, Spodoptera litura(Fabricius), is a major pest of tomato and frequently demands control measures. The timing of insecticide application is a key factor in determining its efficiency, so an experiment was designed to investigate this. Application of insecticide was based on three criteria: (i) the number of trap-caught moths in a Delta-type trap with a commercial sex pheromone lure placed in the center of the target area, soon after plant emergence; (ii) the percentage of plants exhibiting pinhole-type damage (10% or 20%) and (iii) the percentage of plants exhibiting shot hole-type damage (10% or 20%) compared to a check plot without any control measures. We found that the number of trap-caught moths was, compared to the other methods, the best means of deciding on insecticide application in tomato plant to control the tobacco cutworm. Using pheromone traps, we obtained the best performance of the insecticide Shinnago, causing > 90% larval mortality. Without insecticide application, tomato yield reduction due to the tobacco cutworm larva damage was 27%.

This study used both kinesiotaping and extracorporeal shock wave therapy on patients diagnosed with frozen shoulder - a common musculoskeletal disorder in adults - in order to observe the effects on the joint range of motion. 21 adult(male 12, female 9) were selected and distributed into randomized groups. One group received kinesiotaping (n=10) and the other group received kinesiotaping together with extracorporeal shockwave therapy (n=11). After a 6 week duration of receiving kinesiotaping and extracorporeal shockwave therapy, changes in the joint range of motion in the patients were observed. Post-treatment of frozen shoulder, the changes in abduction within the shoulder joint were as follows: in both groups there was a noticeable increase in the joint range of motion (p<.05). Post-treatment of frozen shoulder, the changes in external rotation within the shoulder joint were as follows: both groups showed a significant increase in the joint range of motion (p<.05). The result of suggest that, it can be inferred that both the extracorporeal shockwave therapy and kinesiotaping are effective in increasing the joint range of motion in patients with frozen shoulder.

The purpose of this study was to determine the effects of music therapy and ball exercise on women experiencing menstrual discomforts, thereby identifying the validity of these methods as interventions against menstrual discomforts, with a particular goal of presenting basic data for clinical use. Twenty university students in their 20s were assigned to two therapy groups in a sequence via simple random sampling; ten subjects attended a ball exercise combined with music therapy group and the other ten subjects attended a music therapy group. Ball exercises were conducted 3 times per week for a total of 12 times, starting from 3 weeks before the expected first day of the menstrual period and ending on the last day of the menstrual period. Similarly, the subjects participated in music therapy by listening to music for 35 minutes per session and 3 sessions per week, starting from 3 weeks before the expected first day of the menstrual period and ending on the last day of the menstrual period. Five out of six categories of menstrual discomforts were significantly decreased in both music therapy and ball exercise, the exception being changes in the autonomic nervous system, while those in the music therapy group showed a significant difference only in the category of behavioral changes. The results of the present study demonstrate that the ball exercise combined with music therapy more effective in improving menstrual discomforts than the music therapy group.

We developed a type of sausage made of chicken breast and liquid egg whites for consumers interested in weight management. To determine the quality of the product, its chemical characteristics, fatty acid composition, free amino acid contents, and nucleotides contents were evaluated during 4 weeks of storage. Sensory evaluation was conducted by both general consumers and body-builders. The sausage was proposed as a fat-free product as fat content was 0.12% based on the Korean Indication Standard of Animal Origin Food. Protein content was 13.42% and calorie value was 61.50 kcal/100 g of the sausage. In sensory evaluation, the mixture of chicken breast and egg whites stuffed into the same casing had an adverse effect on taste, color, texture and overall acceptance while the product that contained egg white stuffed separately into the outer casing enclosing the chicken breast (double layer) improved these attributes. The developed double-layer sausage can last for at least 4 weeks of storage without quality deterioration of flavor-related compounds, such as fatty acids and nucleotides.

With the advent of technology, telecollaborative projects have emerged that enable learners to interact with native speakers of the target language, helping them to improve both language proficiency and intercultural communicative competence (ICC). One long-lasting project is Cultura, which has been successful because of its pedagogical qualities, such as a task-based approach and collaborative learning (Furstenberg & Levet, 2014). In this study, a Cultura-inspired project was designed to foster interaction between students of Korean as a foreign language (KFL) residing in Australia and students of English as a foreign language (EFL) residing in Korea. ‘Facebook’ was used for asynchronous communication, and ‘Kakao Talk’ and ‘Facebook’ messenger were used for synchronous online chat. In order to suggest a model for designing and practicing future intercultural projects, this study focused on demonstrating the procedures for each task and examining evidence of ICC in the students’ interactions, as represented in speech acts. This study also investigated the students’ experience of the project, using surveys and interviews. The findings suggest that the students showed limited knowledge of their target culture in a word-association task on Facebook, but that their online interactions provided evidence of ICC, for example curiosity, and reflection on their own culture. Most of the students enjoyed the project, especially the online chat. However, the participants mentioned issues, such as the limited number of online interactions and lack of in-class discussions. For future practice, the use of various modes of communication and the desirability of balancing proficiency levels in the target language are discussed.

Oxalic acid has a nematicidal activity against the root-knot nematode Meloidogyne incognita. High producer of oxalic acid was isolated, and then named as Aspergillus niger F22. Oxalic acid production was investigated under various temperatures from 20 – 33oC and rotational speeds in 5 L jar fermenters. Yield of oxalic acid increased with decreasing temperature. The highest yield was obtained at 23oC, showing the yield of oxalic acid of 8.7 g/L, whereas oxalic acid production was least at 33oC. At 20oC, the yield was lower than that of 23oC. At a rotational speed of 300 rpm, serious oxygen depletion was present from 48 - 72 h, resulting in low productivity of 26.2 mg /L·h. When a rotational speed was set at 600 rpm, dissolved oxygen tension was over 40% and oxalic acid production increased up to approximately 55%. Viscosity during the culture differed with temperatures. Viscosity increased with the increment of temperatures. When A. niger F22 was cultured at 23oC, viscosity was 810 cP, which was favorable for oxalic acid production.

Pheromone biosynthesis activating neuropeptide (PBAN) produced in the subesophageal ganglion is known to stimulate pheromone production in the pheromone gland. A cDNA isolated from female adult heads of Maruca vitrata encodes 197 amino acids including PBAN, designated as Mvi-PBAN, and four other neuropeptides (NPs): diapause hormone (DH) homologue, α-NP, β-NP and γ-NP. All of the peptides are amidated in their C-termini and shared a conserved motif, FXPR(or K)L-NH2 structure. Mvi-PBAN consists of 35 amino acids as previously reported (Chang and Ramasamy, 2014). RT-PCR analysis revealed that Mvi-PBAN cDNA was expressed in all examined body parts. Nucleotide sequence analysis of RT-PCR products indicated the Mvi-PBAN sequence was identical in all examined body parts of both sexes. These results suggest that Mvi-PBAN expression is maintained in examined stages or tissues.

Biological control has been tried for integrated pest management. It is often comparable, safe, and environment-friendly, making itself an alternative for chemical agents. Filamentous microorganisms, i.e., fungi and streptomystes, produce many kinds of useful metabolites, and some of them have been developed as a biocontrol agent. However, they still have a long way because of the concern of manufacturing cost. Therefore, process development was intensively studied to meet cost-effectiveness. Operating conditions of bioreactor, e.g., agitation and aeration, had an effect on biological and physiological responses such as mycelial morphology, oxygen and nutrient transfer. Understanding relationship between operating parameters and microbial responses in terms of growth, substrate and oxygen consumption, and production yield was critical for process development. This study dedicated to build strategies for mass production of biological control agent using aerobic filamentous microorganisms.

The purpose of this study was to identify the effects of Pilates and Tai Chi as physical therapy interventions for pain and dysfunction in chronic lumbago patients. A total of 31 females who were at least 20 years old and diagnosed with chronic lumbago were divided into a Pilates group(PG) (n=10), Tai Chi group(TCG)(n=11), and conservative group(CG)(n=10), to measure their pain and dysfunction before and after a six week intervention. With regard to the changes in the lumbago consciousness scale before and after the six-week intervention, all three groups showed statistical significance(p<.05). The intergroup differences were significant between the PG and CG and between the TCG and CG. All three groups also showed statistical significance(p<.05) in the Oswestry Disability Index before and after the six-week intervention. Again, the intergroup differences were significant between the PG and CG and between the TCG and CG. Based on these results, the application of Pilates and Tai Chi with conservative treatment may be effective in reducing pain and improving dysfunction in chronic lumbago patients.

This study observed the effects of ankle strengthening exercise and whole body vibration on the balance ability of older adults, thereby intending to provide basic materials for intervention methods aimed at improving older adults’ balance ability. The subjects were 20 older adults who had experienced a fall. They were equally divided into two groups. Ankle strengthening training was applied to one group and ankle strengthening training and whole body vibration were applied to the other group, a timed up and go (TUG) test and Tinetti performance oriented mobility assessment (POMA) were performed, and changes in the subjects’ limits of stability were observed. The TUG and POMA results significantly differed between before and after the experiment in the angle strengthening training (AST) group and the angle strengthening training with whole body vibration (ASTWV) group. In addition, the interaction between timing and each group was statistically significant. The limits of stability significantly changed after the intervention in both groups. Differences in the posterior and right limits of stability were significant between the AST group and ASTWV group. Therefore, ankle strengthening exercise and whole body vibration improve older adults’ balance maintenance and reduce falls or the risk factors for falls in older adults.

The purpose of this study is to provide fundamental clinical data for the treatment plan and rehabilitation of patients with cervical hypolordosis by comparing the cervical headache and muscle activity after cervical stretching exercise and cervical traction, which are generally applied to patients with cervical hypolordosis. The research subjects included 20 patients without gender division who were diagnosed with cervical hypolordosis. After applying cervical stretching exercise and cervical traction for six weeks, cervical headache and the activity of the muscles around the cervical vertebra(upper trapezius muscle, sternocleidomastoid muscle, splenius capitis muscle, and anterior temporal muscle) were investigated and the following results were obtained. In a comparison of the within group intervention effects of the two groups, cervical pain statistically significantly decreased in the cervical stretching exercise group. According to the results of analyzing the change of muscle tension in the upper trapezius muscle, both the cervical traction group and showed a statistically significant within group difference in the left and right side. According to the results of analyzing the change in the muscle tension of the splenius capitis muscle, both groups showed a statistically significant within group difference in the left and right side. In a betweengroup comparison, a statistically significant difference in the right side was observed. These results confirm that cervical vertebra traction and cervical stretching exercise decrease the cervical headache and muscle activity of the upper trapezius muscle and the splenius capitis muscle among patients with cervical hypolordosis.

The pheromone biosynthesis in Plutella xylostella is stimulated a neuropeptide, pheromone biosynthesis activating neuropeptide which is produced in subesophageal ganglion. The pheromone production is more active in the scotophase than in the photophase, which suggests that there may be changes of gene expression in the pheromone glands. To analyze gene expression related to pheromone biosynthesis, we performed transcriptomes of pheromone glands which were isolated at every 4 h. Eleven pheromone biosynthesis-related genes, acetyl-CoA carboxylase, fatty acid synthase, acyl-CoA dehydrogenase, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, Δ9 desaturase, Δ11 desaturase, fatty acid reductase, alcohol oxidase, aldehyde oxidase, and aldehyde reductase were identified. Among these genes, the expression of aldehyde reductase and aldehyde oxidase were relatively higher in the scotophase than in photophase, which may affect increase of pheromone production in the scotophase. Expression of signal genes involving in pheromone biosynthesis such as acyl-CoA desaturase, FAR, PBAN receptor, fatty acid transporter and acyl-CoA binding protein did not exhibited any significant difference.

A rapid cold hardening (RCH) and supercooling capacity usually play crucial roles in survival during the overwintering period in the tobacco budworm, Helicoverpa assulta, a freeze-susceptible species. Cryoprotectants such as polyol or sugar affect RCH and maintain fluid status of hemolymph. This study is performed to identify cryoprotectants as a RCH factor in H. assulta. Pre-exposure of H. assulta larvae to 4℃ significantly increased survival at -10℃ in all developmental stages from egg to adult. RCH was dependent on the duration of the pre-exposure period. RCH also significantly enhanced the supercooling capacity. Analysis of cryoprotectants from the hemolymph revealed that the pre-exposure treatment allowed the larvae to accumulate glycerol and trehalose among polyols examined. In addition, unknown materials from 2 peaks were also increased. TIC analysis revealed 3 predicted formulas for unknown materials, C26H24O20S or C3H4N6OS and C22H20O21. Glycerol-3-phosphate dehydrogenase (GPDH) and glycerol 3-phosphatase (G3P) that involving in glycerol biosynthesis were identified from the transcriptome of H. assulta 4th instar larvae. Based on the expression level of transcripts, the expressions of GPDH and G3P were relatively increased when compared to that of the control, suggesting that these genes contribute to overwintering and biosynthesis of glycerol.

The purpose of this study is to observe the effect of elasticity of taping on ankle muscles’activity and endurance after plyometric training that easily causes ankle injury, and provide baseline data for physical therapy intervention methods. The study subjects are 24 male students in their 20s who attend N University in Choongnam. They were divided into three groups; 8 subjects in the elastic taping group, 8 in the non-elastic taping group, and 8 in the non-taping group(control group). They had plyometric training for 6 weeks. After the training, this study measured their maximum voluntary isometric contraction(MVIC) and muscle endurance of the muscles around ankle joint. The experiment result is as follows. After the training, all three groups showed improvement in muscle strength and endurance. The elastic taping group showed insignificant improvement in muscle strength but significant increase in muscle endurance in plantarflexion. In dorsiflexion, both muscle strength and endurance increased significantly. The non-elastic taping group showed insignificant improvement in muscle strength but significant increase in muscle endurance in plantarflexion. Taping during plyometric training had a little or insignificant effect on muscle endurance and strength compared to the non-taping group.

The goal of this study was to assess the effect of a group exercise program on cognitive function of elderly people. Subjects were chosen to be elders with dementia having minor to moderate degrees of cognitive function. Study was started out by randomly dividing the 16 subjects into two groups, each with 8 people; the group exercise group and the control group. The group exercise group performed 8 weeks of group exercise program and general physiotherapy while the control group only performed general physiotherapy. Cognitive function was measured by Korean version mini-mental state examination. The study group's attention and calculation statistically significantly improved but the control group saw no statistically significant change. The group exercise program affected improvement in cognitive function of elderly people with dementia and in particular, was effective for enhancing their attention and calculation.

The purpose of this study was to analysis of the effect of proprioceptor training and vestibular organ training for balance ability. The subjects was consist of two different subjects group, proprioceptor training group and vestibular organ training group. Proprioceptor training group consisted of 10 subjects and vestibular organ training group consisted of 10 subjects. Training was performed 3 times per week, 30 minutes per day, for 3 weeks. Balance ability analysis was performed using Romberg's one leg standing test and BT4 when opened eyes and closed eyes. The analysis results were as follows. There was no significant differences in balance after the training in both groups when they opened their eyes(p<.05). But there was significant differences in balance after the training in both groups when they closed their eyes(p<.05). And there was no significant difference in balance after the training between the proprioceptor training group and the vestibular organ training group when they closed their eyes(p<.05). Given the above results, proprioceptor training and vestibular organ training enhanced balance but there was no significant difference between the two methods.