현재 대한민국의 가족 규모가 축소됨에 따라 반려동물에 대한 관심 및 양육 가구 수가 급증하면서 반려동물과의 상호작용도 활발하게 이루어지고 있는 추세이다. 그러나 이러 한 현실과는 달리 국내에서는 인간-동물 상호작용(Human-Animal Interactions, HAI), 반려동물과 인간의 상호작용에 관한 연구가 많이 이루어지고 있지 않는 실정이다. 본 연 구에서는 반려동물과 인간의 상호작용에 관한 국내 연구들을 살펴보고 정리하여 관련 연 구에 필요한 자료를 제공하는데 목적이 있다.

This study is aimed at applying the philosophical concept of “différance” to contemporary men’s fashion design, which could effectively show the shift in thinking. For the study method, the author examined the theoretical background of deconstruction and prepared the standard of analysis by organizing the concept of “différance,” which exists at the center of philosophy. This study selected the three most popular brands: Maison Margiela, Comme des Garcons, and Yohji Yamamoto. The results are as follows. First, there is expression of perpetual reservation emphasizing time. This includes texture, patchworks, hems with ladder, and exposure of seam that expresses the trace of time flow and delayed delivery of immediate meaning, and the expression creates delay between major and minor and leads to consistent role exchange. Second, the trace of blank that emphasizes the space gives a visual sense of weight on spares with the intentionally granted space, as well as the space that is the trace of trace and creates a sense of depth through the direction of empty space. Third, the space created in the process of wearing cloth is visualized, the structure of clothing is deconstructed, and the movement from the process recomposes the shape of space and expands the definition that enables expansion of time and space. Fourth, the undetermined relationship expresses the border of time and space visually and deconstructs time and space. The approaches are mostly constructive, demonstrating an avant-garde form of clothes-wearing to show the non-form or imbalance condition.

기존의 사회인지와 정서 연구들은 주로 얼굴자극을 이용하여 초기지각단계 및 후기인지과정에의 정서 효과를 조사해 왔다. 그러나, 정서의 효과가 정보처리과정의 각 단계에서 어떤 양상으로 나타나는지와, 정서유형에 따른 효과 양상의 변화 여부는 불확실하다. 본 연구에서는 얼굴 대신 생물형운동자극을 이용해 자극에 내포된 행복, 분노, 중립정서가 지각과제와 작업기억과제 수행에 미치는 영향을 조사하였다. 참가자는 연달아(지각과제) 또는 시간차를 두고(작업기억 과제) 제시되는 두 생물형운동의 동일 여부를 판단하였다. 지각과제에서는 정서가를 가진 자극 시행의 정확도가 중립정 서자극에 비해 낮아 정서정보가 초기지각처리에는 부정적 영향을 미치는 것으로 보였으나, 작업기억과제에서의 기억정보유지에는 도움이 되는 것으로 나타났다. 또한, 각 과제에서 정서유형에 따라 다른 수행 양상이 관찰되었다. 분노정서는 지각단계에서 더 많은 정신적 자원을 요구하여 부하가 증가할 경우 정확도가 낮아지지만 기억유지에는 긍정적으로 작용하는 것으로 보이며. 행복정서의 경우 중립정서가 이어서 제시될 때 이를 행복정서와 유사하게 처리하려는 편향을 유도하는 것으로 보인다. 본 연구는 생물형운동자극을 이용하여 정보처리과정에서의 정서 영향을 재확인하였고, 처리 단계별 및 정서 종류별로 다른 양상의 영향이 나타난다는 점을 추가로 밝혀, 정서정보의 정교한 조작 및 통제를 위한 유용한 단서를 제공한다.

In most mammals, metaphase II (MII) oocytes having high maturation promoting factor (MPF) activity have been considered as good oocytes and then used for assisted reproductive technologies including somatic cell nuclear transfer (SCNT). Caffeine increases MPF activity in mammalian oocytes by inhibiting p34cdc2 phosphorylation. The objective of this study was to investigate the effects of caffeine treatment during in Vitro maturation (IVM) on oocyte maturation and embryonic development after SCNT in pigs. To this end, morphologically good (MGCOCs) and poor oocytes (MPCOCs) based on the thickness of cumulus cell layer were untreated or treated with 2.5 mM caffeine during 22-42, 34-42, or 38-42 h of IVM according to the experimental design. Caffeine treatment for 20 h during 22-42 h of IVM significantly inhibited nuclear maturation compared to no treatment. Blastocyst formation of SCNT embryos was not influenced by the caffeine treatment during 38-42 h of IVM in MGCOCs (41.1-42.1%) but was significantly improved in MPCOCs compared to no treatment (43.4 vs. 30.1%, P<0.05). No significant effects of caffeine treatment was observed in embryo cleavage (78.7-88.0%) and mean cell number in blastocyst (38.7-43.5 cells). The MPF activity of MII oocytes in terms of p34cdc2 kinase activity was not influenced by the caffeine treatment in MGCOCs (160.4 vs. 194.3 pg/ml) but significantly increased in MPCOCs (133.9 vs. 204.8 pg/ml). Our results demonstrate that caffeine treatment during 38-42 h of IVM improves developmental competence of SCNT embryos derived from MPCOCs by influencing cytoplasmic maturation including increased MPF activity in IVM oocytes in pigs.

In this study, we investigated the macrobenthic community structure and spatiotemporal variations in Uljin Marine Ranching area, East Sea of Korea. Macrobenthos were collected using a modified van Veen grab sampler from April to September 2013. Total number of species sampled was 345 and mean density was 5,797 ind. m-2, both of which were dominated by the polychaetes. The most dominant species were Spiophanes bombyx (53.64%), followed by Magelona sp.1 (6.96%), Cadella semitorta (2.73%), Lumbrineris longifolia (2.16%) and Alvenius ojianus (2.08%). Cluster analysis and nMDS ordination analysis based on the Bray-Curtis similarity identified 2 station groups. The group 1 (station 2, 3, 5, 6, 8 and 9) was characterized by high abundance of the polychaetes Magelona sp.1, Lumbrineris longifolia, Scoloplos armiger, Praxillella affinis, Maldane cristata and the bivalve Alvenius ojianus, with fine sediment above 30m water depth. On the other hand, the group 2 (station 1, 4, 7 and 10) was numerically dominated by the polychaete Lumbrineriopsis sp. and the bivalve Cadella semitorta, with coarse sediment below 5m water depth. Collectively, the macrobenthic community structure showed a distinct spatial trend, which seemed to be related to the water depth and sediment composition.

본 연구에서는 DSM을 이용하여 단백질 추출이나 정제 없이 식품에 적용 가능성이 있는 가식성 필름을 개발하였다. HPH 처리는 표면이 매끄럽고 균일한 형태의 필름을 형성시켰고, 필름의 WVP값을 감소시켜 수분 방벽 효과를 높였다. 필름 내 글리세롤 농도 증가는 WVP값을 전반적으로 높였다. 농산물 가공 부산물인 DSM으로 제작된 가식성 필름은 새로운 식품 소재로서 상업적 적용 가능성을 보여주었다.

본 연구에서는 자연분해재 펠릿과 polyethylene들을 이용해 LDPE와 신장율과 인장강도가 유사한 생붕괴성 플라스틱 필름들을 제조하였다. 식품 포장재로서의 안전성, 광분해성, 열분해성 및 곰팡이 생분해성을 평가한 결과 제작된 생붕괴성 플라스틱 필름들은 식품 포장재로 사용하기에 안전하고 LDPE 필름에 비해 상대적으로 우수한 분해 특성을 나타내어 환경친화적인 식품 포장 소재로 사용될 가능성을 보여주었다.

This study examined the maturation and main spawning season of Japanese spear lobsters, Linuparus tirgonus(Von Siebold) captured around Jeju island from January to September, 2008. Carapace length(CL), body weight(BW) and gonad weight(GW) were measured. Gonadosomatic index(GSI) and sex ratio were calculated. In female group, CL showed the highest value in June and decreased after August. GW showed a peak in July and decreased rapidly after August. The mean gonadosomatic Index(GSI) reached a maximum value between June and August. Number of egg ranged from 143,360 to 189,504.

The development of soy cutlets containing textured soy protein (TSP) as a meat analog was studied. In order to decrease the beany flavor and to increase the texture, TSP was treated with 0.3% Flavourzyme or 0.1% Protamex for 10 or 20 min, respectively. The degree of hydrolysis for TSP treated with Protamex was higher than that treated with Flavourzyme. Hydrolysis was observed to increase as the reaction time was increased for both Flavourzyme and Protamex. The water holding capacity of TSP treated with Protamex for 10 min was the highest, and that treated with Flavourzyme for 20 min was similar to that of Protamex treatment for 20 min. The oil binding capacity of TSP treated with Protamex for 20 min was the highest. The hardness of the soy cutlets using TSP treated with Flavourzyme for 10 min was higher than that treated for 20 min, while that of Protamex treated for 20 min was higher than that treated for 10 min. The cohesiveness of the soy cutlets using TSP treated with Flavourzyme or Protamex for 10 min was higher than those treated for 20 min. The chewiness of the soy cutlets treated with Flavourzyme for 10 min was higher than for those treated for 20 min, while those treated with Protamex for 20 min was higher than those treated for 10 min. The springiness of TSP treated with Flavourzyme for 20 min was higher than those treated for 10 min, and higher than those treated with Protamex for 10 or 20 min. For sensory evaluation, the beany flavor of the soy cutlets treated with Protamex for 20 min was the weakest. The flavor and chewiness of both a pork cutlet and a soy cutlet treated with Protamex for 20 min were the best. In the overall quality, soy cutlets treated with Protamex for 20 min was the most desirable. In conclusion, soy cutlets treated with 0.1% Protamex for 20 min could be a reasonable substitute of pork cutlets.

Background : The objective of this study was to make colloidal dispersion of the active compound was characterized nano-composite from radix of Angelica gigas Nakai using hot melt extrusion (HME). Therefore, food grade hydrophilic polymer matrices was used to disperse compound in aqueous media. Methods and Results : Extrudate solid formulations (ESFs) mediated various HPMCs (hydroxypropyl methylcellulose) and Na-Alg polymer from ultrafine powder of radix of Angelica gigas Nakai was developed through physical crosslink method (HME) with ionization agent (treatment of acetic acid) and food grade polymer [HPMCs such as HP55, CN40H, AN6 and sodium alignate (Na-Alg)]. X-ray powder diffraction (XRD) analysis confirmed the amorphization of crystal compounds in extrudate solid formulations mediated HP55 (HP55-ESF). Differential scanning calorimetry (DSC) analysis indicated a lower enthalpy (ΔH = 10.62 J/g) of glass transition temperature (Tg) in HP55-ESF. Infrared fourier transform spectroscopy (FT-IR) reveled new functional groups produced in HP55-ESF. Phenolic compound, flavonoid (including decursin and decursinol angelate) and antioxidant activity increased at 5, 10 and 2 times in HP55-ESF. Water solubility (61.5%) nano size (323 ㎚) particle was achieved in the HP55-ESF. Conclusion : Nano-composites have been developed utilizing melt-extruded solid dispersions technology by food grade polymer enhanced nano dispersion (< 500 ㎚) of active compound from radix of Angelica gigas Nakai with enhanced solubility and bioavailability. This nano-composites of radix of Angelica gigas Nakai developmental and marketed products to enable therapeutic performance.

Astragalus membranaceus is a well known oriental medicinal herb. The polysaccharides of the aboveground parts (AMA) and the radix (AMR) of A. membranaceus are the most important functional constituents. Methods and Results: The aim of this study was to determine the effects of AMA and AMR on the oxidative damage induced in the skeletal muscle of rats subjected to exhaustive exercise. Sprague-Dawley rats were randomly divided into exercise and non-exercise groups; in the groups receiving the test compounds, AMA and AMR were administered orally for 30 days. Skeletal muscle samples were collected from each rat after running to exhaustion on a treadmill to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) and the concentation of malondialdehyde (MDA). The antioxidant enzyme activities of SOD and GSH-Px of skeletal muscle of AMA- and AMR-treated groups were significantly higher than those of the control and commercial sports drink (SPD)-treated groups in exhaustive exercise rats. In addition, MDA concentrations in the skeletal muscle of the AMA- and AMR-treated groups were significantly lower than those of the control and SPD-treated groups. In the present study, the effects of AMA and AMR on exercise endurance capacity were also evaluated in mice subjected to a swimming exercise test. AMA and AMR supplementation prolonged the swimming time of mice and enhanced exercise endurance capacity. AMA and AMR possess the ability to retard and lower the production of blood lactate, and prevent the decrease of serum blood glucose. Conclusions: These results showed that, AMR and AMA exerted beneficial effect in mice, increasing the activity of the antioxidant systems and inhibiting oxidative stress induced by exhaustive exercise. The compounds improved exercise performance and showed anti-fatigue effects against exhaustive exercise.

Background: This study were to investigate the effect of Pediococcus pentosaceus fermented Radix astragali (AMRP) and non-fermented products (AMRNP) on collagen synthesis in the cultures of human dermal fibroblasts, and their inhibitory effects on the matrix-degrading enzymes (collagenase, elastase, and gelatinase). Methods and Results: Both AMRP and AMRNP significantly improved cell growth and proliferation of HDF cells. However, the enzyme-linked immunosorbent assay and Western blot analysis demonstrated that AMRP, but not AMRNP, significantly and dose-dependently stimulated the biosynthesis of type I procollagen in both aged (74 y) and young (21 y) HDF cells. Real-time reverse transcription-polymerase chain reaction revealed that expression of type I, type III procollagen and transforming growth factor β1 (TGF-β1) mRNA was significantly stronger in AMRP-treated HDF cells than that of AMRNP-treated and un-treated HDF cells. The AMRP revealed an increase in astragaloside Ⅳ only depending on increase in fermentation period, because other astragalside converted to astragaloside Ⅳ, which it detached acyl group by fermentation processing of Pediococcus pentosaceus. Conclusion: The results also suggested that AMRP could stimulate the collagen biosynthesis in human dermal fibroblasts, which is, associated with the regulation of procollagen biosynthesis resulting from AMRP-induced TGF-β1 expression and the mitogenic activity in HDF cells, and therefore, is expected to reduce the age-dependent loss of extracellular matrix proteins.

Background: Polysaccharides are the most important functional constituent in Astragalus membranaceus. The purpose of the present study was to evaluate the effect of polysaccharides isolated from the aboveground parts of A. membranaceus (AMA) and polysaccharides isolated from the roots of A. membranaceus (AMR) immune function by modulated cytotoxic T cell and Th1- and Th2- related cytokines kinetics. Methods and Results: Sprague-Dawley rats were randomly divided into exhaustive exercise case groups and non-exercise case, AMA and AMR samples were administered orally for 30 days (500 ㎎/㎏/day and 10 ㎎/㎏/day, respectively) and were compared to those rats in the groups fed commercial sports drink (SPD) and vehicle. Both exhaustive exercise groups and non-exercise groups had a lower ratio of CD4+ and CD8+ cells in the spleens of the rat fed AMA and AMR compared to those in the rats fed SPD and vehicle group. These results suggested that AMA and AMR promote an increase in the proportion of cytotoxic T cells. The IL-4- producing T lymphocytes decreased significantly in the AMR (10 ㎎/㎏/day) group compared to SPD and vehicle, whereas the AMA group increased the IL-4 concentration more than the SPD and vehicle in exhaustive exercise group. However, the populations of IFN-γ-producing T lymphocytes of AMR and AMA increased. AMA decreased the concentration of IFN-γ to inhibit the Th1 response and thereby increased the concentration of IL-4 to induce a Th2 response that was related to humoral immunity in the non-exercise group. Conclusions: These results showed that, in addition to Th1/Th2 regulation, AMR and AMA played an important immuno-modulatory role after exhaustive exercise-induced Th1/Th2 lymphocyte imbalance, which might be correlated with cytokine producing immunoregulatory cells.

Backgrounds : The action mechanisms of several chemopreventive agents derived from herbal medicine and edible plants have become attractive issues in cancer research. Tea is the most widely consumed beverage worldwide. It have been demonstrated that the active principles of tea sources such as flower extract Camellia sinensis (CSF) and Camellia japonica (CJF)were attributed to their tea polyphenols. We focused on investigating CSF, CJF, mixtures of CSF and CJF has been proven to suppress colonic tumorigenesis. Methods and Results : In this study, human colorectal carcinoma HT-29 cells were treated with CSF, CJF, mixture of CSF and CJF to examine the anti-proliferative and pro-apoptotic effects of mixture of CSF and CJF (3 : 1), as well as the molecular mechanism underlying these effects. Cell viability assay, nuclear staining, DNA fragmentation, caspase assay, cytochrome c release, were utilized to dissect the signaling pathways. In mixture of CSF and CJF (3 : 1), CSF appeared most anticancer effect by both MTT assays and the cleavage analysis of apoptosis-related molecules and PARP. Interestingly, we found that CJF make it possible to express the apotosis inducing by CSF in a short time and apoptosis effect of CSF maintained sustainable. Conclusion : In summary, our results from this study suggest that in HT-29 human colon cancer cells (i) CSF treatment causes damage to mitochondria, and (ii) CJF contributed CSF induced apoptotic cell death mediates cytochrome C release, (ⅲ) mixture of CSF and CJF (3 : 1) the potential to function as a chemopreventive agent against colon cancer.

Backgrounds : Camellia sinensis is known to have a very high antioxidant activity, but its petals are small and it is difficult to use it because of its low yield. In comparison Camellia japonica has many petals and yield, however, the biological effects of C. japonica have been less frequently studied. In the present study, we focused on investigating the in vitro antioxidant effect of the ethanol extract from flower of C. sinensis (CSF), C. japonica (CJF) and mixture of CSF and CJF. Methods and Results : Content of total phenolics and total flavonid, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activities, reducing power, superoxide anion and hydroxy radical scavenging activity of CSF and CJF were compared in vitro experimetal models. Total phenolics was contained the higer in CJF (172.19±1.65 mgCAE/gEX) than 146.75±0.15 mgCAE/gEX in CSF. And effects of antioxidant measured by reducing power, superoxide anion generated by xanthine/xanthine oxidase and hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2) in a cell-free system was shown higher in mixture of CSF and CJF than BHT, ascorbic acid as a chemical oxidant which was detected by electron spin resonance spectrometry coupled with DMPO spin trapping. These results suggest that Camellia flower extract such as CSF and CJF exhibits antioxidant properties by scavenging ROS. Camellia extract contained quercetin, quercetin-3-O-glucoside, quercitrin and kaempferol, which are antioxidant compounds. Conclusion : As a result, the combination of CSF and CJF showed higher antioxidative effect than using CSF or CJF alone.

Background: Planting vigorous cuttings that quickly develop shoots and roots is essential to the biological and economic success of producing medicinal flowers. The present study aimed to evaluate the effect of storage temperature and duration on seedling capacity in the propagation of Chrysanthemum indicum L. and to investigate the effect of rooting media on the growth of C. indicum L. after cutting.Methods and Results:Returning cuttings to supplemental cold storage (2.0 ± 1.0°C) may extend duration of cutting viability 6 weeks, returning cuttings to supplemental warm storage (25.0 ± 1.0°C) is not recommended. The treatment of the growing media experiments, which were conducted in the 2014 planting seasons, included sawdust, river sand, topsoil + sawdust, topsoil + poultry manure, sawdust + river sand, river sand + poultry manure, topsoil + river sand + poultry manure, topsoil + poultry manure + river sand + sawdust. Result indicated that the topsoil + poultry manure media performed best and supported the highest number of branches (3.47), branch length (26.39), and number of leaves (88.63).Conclusions:The results of the present study suggest that cold storage and the topsoil + poultry manure growth media was superior in supporting the early establishment of C. indicum cutting, this result will have a tremendous influence on propagation of this species.

Background: In recent years, adjuvants have received increasing attention owing to the development of purified subunit and synthetic vaccines which are poor immunogens and require additional adjuvants to evoke an immune response. Therefore, immunologic adjuvants have been developed and tested. Plant polysaccharides have been recognized as effective biological response modifiers with low toxicity.Methods and Results: In this study, the polysaccharide from the aboveground part of Astragalus membranaceus Bunge containing immunomodulating arabino-3,6-galactan was evaluated for its hemolytic activity and adjuvant potential in the specific cellular and humoral immune responses to ovalbumin. The polysaccharide from the aboveground part of Astragalus membranaceus Bunge was co-immunized with the purified Vi capsular polysaccharide of Salmonella typhi vaccine in mice. The polysaccharide from the aboveground part of Astragalus membranaceus Bunge did not induce any hemolytic activity or side effects at doses up to 500㎍/㎖. The concanavalin A-, lipopolysaccharide-, and ovalbumin-induced splenocyte proliferation and serum ovalbumin-specific IgG, IgG1 and IgG2b antibody titers in immunized mice were significantly enhanced by AMA. Pharmacological data revealed that the polysaccharide from the aboveground part of Astragalus membranaceus Bunge increased antigen-specific antibody levels in immunized mice. The polysaccharide from the aboveground part of Astragalus membranaceus Bunge-adjuvanted purified Vi capsular polysaccharide of Salmonella typhi vaccine improved the proliferation of splenocytes and macrophages as well as stimulated cytokine production.Conclusions: These results suggest that the polysaccharide from the aboveground part of Astragalus membranaceus Bunge-adjuvanted vaccines enhanced humoral and cellular immunity and that the polysaccharide from the aboveground part of Astragalus membranaceus Bunge is a safe and efficacious adjuvant candidate suitable for use in prophylactic and therapeutic vaccines.

Background : Plants are the rich source of antioxidants, which plays a very important role in maintaining human health. Their antioxidant property protects cells of different organs of human beings against free radicals and free radical mediated diseases. Even though, there is lack of knowledge on the antioxidant effect of lutein present in plants. In the present study, lutein was isolated from the GreenTea leaves (Camellia sinensis) which is used as a dietary source. Methods and Results : The procedure adopted for the isolation and purification of lutein using acetone extraction and preparative high performance liquid chromatography (HPLC) is simple and less time consuming. Free radicals scavenging activity of isolated lutein from acetone extract of GreenTea was assessed by DPPH radical scavenging assay and reducing power. The isolated lutein scavenged 79% of DPPH radicals at 20 ㎍/㎖ and two fold lower concentration compared to the standard antioxidants (α-tocopherol). No significant differences were found between the reducing power of the lutein and BHT when their concentrations were high. However, significant differences were observed at relatively low concentrations, the reducing power of lutein was isolated from the GreenTea leaves was stronger than those of their acetone extract and standard antioxidants (BHA). Both electron spin resonance (ESR) and in vitro assay confirmed that lutein was isolated from the GreenTea leaves, exhibited a greater capacity for scavenging superoxide (O2 •－) and hydroxyl (OH •) radicals than standard antioxidants β-carotene and α-tocopherol respectively. Conclusion : The results proven that lutein isolated from GreenTea leaves has an efficient antioxidant ability, it could serve as an antioxidant to scavenge reactive oxygen species.