This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4 showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.

To develop the practical cultivation for paddy field, we investigated the properties of paddy soil, growth characteristics and ginsenoside content of 6-year-old ginseng, Cheonpung variety between poor drainage class (PDC) and imperfect drainage class (IDC). Groundwater level in PDC showed monthly small changes of 20~30 cm, while IDC showed monthly great changes of 28~71 cm depending on rainfall. Soil moisture content in PDC and IDC was 17.2%, 22.5%, respectively. Air temperature in IDC was lower than 0.3℃, while soil temperature was higher than 0.8℃ compare to PDC, respectively. Main soil color of PDC was grayish olive, while IDC was brownish olive. PDC showed yellowish mottles only at underground of 20~40 cm, while IDC showed that at underground of 30~90 cm. IDC showed lower pH, EC, potassium, calcium and magnesium content, but higher organic matter, phosphate, and iron content than that of PDC, respectively. All of EC, organic matter, potassium, calcium, and magnesium content were decreased, but iron content was increased at the subsoil layers of PDC. All of EC, organic matter, phosphorus, and potassium content were decreased, but calcium and magnesium content were increased at the subsoil layers of IDC. Root yield in IDC was more increased by 33% than that of PDC. The moisture content and rusty ratio of ginseng root in IDC were lower than that of PDC. Ginsenoside content in IDC was higher than that of PDC because the ratio of lateral and fine root showing relatively high content of ginsenoside was higher in IDC than that of PDC.

Growth characteristics, root yield and ginsenoside contents of 3-year-old ginseng in greenhouse shaded by 30˚ sloped-curtain made of aluminum were compared to traditional shade facility in order to develop cultural practice for organic ginseng. Light transmittance ratio in greenhouse with 30˚ sloped-curtain shade was distinctly lower than that of traditional shade from sunrise to 9 a.m., while its ratio in greenhouse was higher than traditional shade since 9 a.m. due to the reflection of light. Air temperature of greenhouse was 1.3℃ higher than that of traditional shade on the first ten days of August due to more reflected light. Root yield of greenhouse was 44% higher than that of traditional cultivation because of the inflow of reflected light and the decrease of disease of Alternaria and Anthracnose by blocking rainfall. Dry matter partitioning ratio of rhizome and lateral root were increased in ginseng cultivated at greenhouse due to longer survival time in leaf than traditional cultivation. Total ginsenoside contents cultivated at greenhouse was decreased in the part of taproot, while it was increased in the part of lateral and fine root compare to traditional cultivation. Individual ginsenoside contents between greenhouse and traditional cultivation showed significant difference more frequent in fine root than taproot and lateral root. Total ginsenoside contents including Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, and Rg2 in whole root of 3-year-old ginseng did not showed significant difference by greenhouse and traditional cultivation.

본 연구는 실내식물의 시각적 이미지가 주는 심리적 효과와 식물에 대한 관심도와의 관계， 식불에 대한 관심도와 중요도가 충성도에 미치는 영향을 분석하기 위해 실시히였다. 9종의 실내 식물에 대한 14쌍의 이미지 감성형용사 어휘는 평가적인, 활동적인 그리고 정서적인 요인의 세 가지 주요인이 추출되었다. 추출된 세 가지 이미지 감성요인 중 활동적인 요인보디는 평가적인 요인이, 평가적인 요인보다는 정서적인 요인이 실내 식물의 관심도에 미치는 영향이 상대적으로 더 높은 것으로 나타났다(p<.05). 또한 대상자들의 실내식물을 키우는 것에 대한 관심도는 충성도에 매우 긍정적인 영향을 주는 것으로 나타났으나(p<.05) ， 연간 구입 개수，구입 비용，실내 식물을 키우는 형태와 같은 행동적 요소는 경우에 따라 유의한 차이가 없는 것으로 나타났다. 실내 식물에 대한 중요성의 인식에 있어서 실내식물에 대한 22항목의 중요도는 6가지 주요인이 추출되었다. 추출된 요인 종 삶의 질적 향상을 위한 "정서이완성”，“심미성”의 개념에 해당하는 항목들의 경우 다른 요인에 비해 충성도에 더 긍정적인 영향을 주는 것으로 나타났다(p<.05). 그러나 5가지 충성도의 항목 중 “실내 식물 구입에 더 많은 비용을 투자할 용의가 있다"는 항목은 다른 항목에 비해 충성도가 상대적으로 낮은 것으로 나타났다. 본 연구를 통해 각 식물의 시각적 이미지가 주는 심리적 효과， 실내식물에 대한 관심도，그리고 중요성 인식은 거주자의 정서적 건강 증진과 실내 식물의 소비를 높이는 데 긍정적인 영향을 줄 수 있는 실내식물에 대한 충성도를 향상시킬 수 있을 것으로 판단된다.

This study was conducted to examine major growth responses, the production and partitioning of dry matter on different growth stages, and yields and to select the optimal shading material in both quality and productivity of ginseng. Two cultivars of ginseng, Cheonpoong and Geumpoong, were cultivated in the paddy soil with three different shading materials; three-layered blue and one-layered black polyethylene (TBP), blue polyethylene sheet (BPS), and aluminium-coated polyethylene sheet (APS). Plant heights were linearly increased until June 24 and then maintained with showing higher height in Cheonpoong than that in Geumpoong cultivar. Root lengths were gradually increased until October 16. They were longer in Cheonpoong than that in Geumpoong cultivar, showing slightly longer with APS compared to TBP and BPS. The ability of producing dry matter of leaves was much higher from April to June compared to those of other growth periods, whereas its ability of root was concentrated from the end of June to the end of August. Among the shading materials, the ability of producing dry matter of shoot was higher with TBP than those with BPS and APS, while its ability of root was not appeared certain tendency unlike the shoot. The yield of ginseng roots was the highest with TBP among three shading materials and it was higher in Cheonpoong than that of Geumpoong cultivar. The shading materials which affect the light intensity and the temperature would be considered as an important factor to get better quality and productivity of Korean ginseng.

Root yield and quality of ginseng cultured in paddy soil was low relatively compared with that of upland soil because of moisture injury in root during rainy season. Drainage class in soils generally divided into 6 classes, and it is possible to cultivate ginseng practically in imperfectly drainage class (IDC). This study carried out to select the varieties that is suitable for paddy soil, which is easy to be generated rusty-colored root and physiological-discolored leaf. Experiment plot arranged with the condition of soil humidity contents such as poorly drainage class (PDC) and imperfectly drainage class (IDC), and upland soil. Growth characteristics and root yield were investigated in four-year-old ginseng of varieties, Cheonpoong (CP), Yeonpoong (YP), Hwangsookjong (HS), and Jakyeongjong (JK). CP among four varieties showed the highest yield in IDC and CP was the lowest ratio in leaf discoloration and rusty-colored root. HS was followed by CP in the order of root yield, but it had the weakness that the ratio of rusty-colored root was high respectively.

It's crucial to control Alternaria blight and Anthracnose emerging mostly on ginseng leaves during the rainy season to increase the organic ginseng products. The purpose of this study is to investigate the efficay of lime-brodeaux spray on the ginseng leaves and evaluate the growth and yield of the ginseng, and the contents of ginsenoside and 70% ethanol extracts from 3-year-old ginseng variety, Cheonpoong. Lime-bordeaux sprayings were conducted in the ratio of 6-6 in June, 8-8 from July to September every 15 days. After June 10, the spraying have no effects on the growth leaf and stem, and there was no significant increase in chlorophyll contents. The ratio of intact leaf and root were distinctly increased because Alternaria blight and Anthracnose were decreased by spraying lime-bordeaux mixture. Root weight per plant and root yield were increased by 15%, and 62% in 3-year old ginseng, respectively, because the ratio of intact leaf and root were higher by using lime-bordeaux mixture. Furthermore, spraying of lime-bordeaux mixture is prone to increase the ratio of rusty root in ginseng. Spraying of lime-bordeaux mixture decreased both of the contents of ginsenoside and 70% ethanol extract by 13.7%, and 15.2% in 3-year-old ginseng, respectively.

인삼 논재배 안전생산기술을 개발하기 위해 배수불량지와 배수약간불량지 논토양에서 청3+흑1중직 차광망, 청색차광지 및 은박차광판 해가림을 처리하여 3년생 천풍의 생육특성 및 진세노사이드 함량을 조사한 결과는 다음과 같다. 해가림 유형별 투광량과 기온은 차광지 > 은박차광판 > 차광망 순이었다. 배수불량지의 토양수분장력은 평균 64mbar (절대토양수분함량 25%)로 과습한 조건이었으며, 배수약간불량지에서는 평균 123 mbar (절대수분함량 17%)이었다. 배수불량지의 토양수분장력은 계절과 해가림 유형별 뚜렷한 차이를 보이지 않았지만 배수약간불량지에서는 60~200 mbar의 범위에서 계절적 변화를 보였으며, 해가림유형에 따라 약간의 차이를 보였다. 배수불량지의 수량성은 황증과 적변 발생율의 증가로 배수약간불량지보다 현저히 감소되었다. 해가림 유형별 수량성은 배수불량지에서 차광지가, 배수약간불량지에서 차광망이 가장 높았다. 적변 발생율은 배수등급과 해가림 유형에 따라 일정한 경향이 없었다. 배수불량지는 Panaxatriol 계열 (Rg1, Re 및 Rf)의 진세노사이드 함량이 감소되고 Panaxadiol 계열 (Rb1, Rc 및 Rd)의 함량이 증가되었다. 총 진세노사이드 함량은 배수불량지보다 배수약간불량지에서 높았으며, 해가림 유형별 총 진세노사이드 함량은 배수등급에 관계없이 차광지에서 가장 높았다.

This study was to set the guidelines of soil chemical components in order to assure the safety and quality ofthe panax ginseng from physiological disorder. The disorder symptoms appeared on the leaf with yellow spot, atrophy, yel-low-brown spot, also showed red skin and rough skin of the root. Occurrence type of physiological disorder in cultivatedfield divided into two types:type I ‘such as, yellow spot’ consist of single disorder symptom; type II ‘such as, yellow spotand yellow-brown spot’ consist of two or more different disorder symptoms. The individual contribution of soil properties tothe occurrence type was as follows ; The yellow spot was affected by Na>NO3-N>salinity (EC) in soil. The same results wasobserved in red skin. Atrophy was affected by NO3-N>salinity (EC)>Ca>Mg. Rough skin was affected by P2O5>pH>Organic material > K. It showed positive associated to P2O5, pH and K, but negative associated to organic matter. Simulta-neous occurrence of two different disorder, including cases which yellow spot and yellow-brown spot, those were affected byNO3-N > salinity (EC) > Na > Mg. In the case of atrophy plus yellow-brown spot, those also were affected by in the order :NO3-N > salinity (EC) > Ca > Mg > Na. Red-rough skin was affected in the order : salinity (EC) > NO3-N > K > Na. Soilchemical components appear to be related to occurrence of physiological disorder, particularly in salinity (EC) and NO3-N.The salinity (EC) and NO3-N were negative related to plant growth. In addition, exchangeable cation capacity play criticalroles in attributing to complex occurrence of physiological disorder.

The purpose of this study was to identify soil physical characteristics as guideline for high yield potential inginseng cultivated field which produced 6 years root. Harvest yields of ginseng to be divided by parent rock was in order ofphyllite and red shale 3.1㎏/3.3㎡ > granite and gneiss·schist 3.0㎏ > basalt 2.6㎏ > porphyry 2.2㎏ in upland and forestsoil. Also, with classified by topography, it was in order of foot slope and alluvial fan 3.2㎏/3.3㎡ > valley 3.0㎏ > low hill2.9㎏ > hill, lave flow and dilluvial terrace 2.8㎏ in survey tilth. Class determination of soil texture, it was in order of sandyloam 3.1㎏/3.3㎡ > loam and silt loam 3.0㎏ > clay loam 2.9㎏ > silt clay loam 2.8㎏ in survey tilth. Slope condition offarming land, in case of sloping (2~7%), it was 3.1㎏/3.3㎡ but deep sloping (15~30%) caused decreasing harvest yield. Indrainage classes (excessively, well and moderately well), there was no significantly different in harvest yields. Relationshipbetween harvest yield and soil series, Production sites as yielding 3.0㎏/3.3㎡ were seven sites, also it was contained 14 soilseries. Production sites as yielding 2.5~3.0㎏/3.3㎡ were eleven sites, it was contained 16 soil series. Production sites asyielding 2.0~2.5㎏/3.3㎡ were 10 sites, it was contained 4 soil series.

This study was carried out to select economical byproduct composts as the substitute for the traditionalorganic fertilizer, Yacto, in the cultivation of ginseng seedlings, and to investigate the application method of a selected com-post. Among tested byproduct composts, popped rice hull compost was the best substitute for Yacto, while the application ofdomestic animal manure composts resulted in red skinned roots of ginseng seedlings. Optimal mixing ratio of the poppedrice hull compost with virgin soil (fine sand) were 3~4:1 in bulk, showing the same root yield compared to that of conven-tional seedbed soil. When the popped rice hull compost was lower than 1±0.1% in nitrogen content, the expeller cake of oilseed was added to seedbed soil to rise nitrogen content until 1±0.1%.

To study the optimal cultivation for paddy soil, growth characteristics and ginsenoside content was investigated by both of poor drainage class (PDC) and imperfect drainage class (IDC) in three-year-old ginseng of varieties, Cheonpoong (CP), Yeonpoong (YP), Hwangsookjong (HS), and Jakyeongjong (JK). Root yield of IDC was higher than that of PDC by 3.6 times because stem length, leaf area, and chlorophyll contents were increased, while discolored leaf ratio was decreased. Root yield of HS in PDC was highest among four varieties because chlorophyll contents, leaf area, and survived plant ratio were relatively high. Root yield of CP in IDC was highest among four varieties because of high leaf area and survived plant ratio, and low discolored leaf ratio. Ratio of rusty-colored root showed significant difference by varieties, which was the highest in HS and the lowest in CP among four varieties irrespective of drainage classes. Total ginsenoside contents showed significant difference by drainage classes, which were high in IDC of good growth and low in PDC of poor growth. Total ginsenoside contents were high in JK and CP, while low in HS and YP both of drainage classes.

논토양에서 적응하는 품종 선발과 재배기술을 개발하기 위해 배수등급이 다른 논토양(배수 불량지와 배수 약간불량지)에서 천풍, 연풍, 황숙종, 자경종 등 4 품종을 사용하여 2년생 인삼의 생육특성 및 수량성과 뿌리의 진세노사이드 함량을 조사한 결과는 다음과 같다. 1. 배수등급에 따라 지상부 생존율과 황증 발생율은 뚜렷한 차이를 보여 배수불량지에서는 지상부 생존율이 저하되고 황증 발생율이 증가하였는데, 배수불량지에서 황숙종은 지상부 생존율이 가장 높고 황증 발생율이 가장 낮았으며, 연풍은 지상부 생존율이 가장 낮고 황증 발생율이 가장 높았다. 2. 배수불량지에서는 배수약간불량지에 비해 지상부가 일찍 고사된 관계로 주당 근중이 작고 수량성도 뚜렷이 감소되었는데, 배수불량지에서의 수량성은 자경종 > 천풍 > 연풍 > 황숙종 순으로 자경종과 천풍의 수량성이 높았으며, 배수약간불량지에는 천풍 > 연풍 > 자경종 > 황숙종 순으로 천풍과 연풍의 수량성이 높았다. 3. 적변율은 배수등급보다는 품종 간에 더 큰 차이를 보였는데, 천풍과 자경종의 적변율은 비교적 작은 반면 연풍과 황숙종은 매우 큰 특징을 보였다. 4. 근 비대가 불량했던 배수불량지는 배수약간불량지보다 진세노사이드 함량이 증가되었는데, 배수불량지에서 품종별 총 진세노사이드 함량은 연풍 > 천풍 > 자경종 > 황숙종 순이었고 배수약간불량지에서는 연풍 > 자경종 > 천풍 > 황숙종 순으로 배수조건에 관계없이 연풍이 가장 높고 황숙종이 가장 낮았으며, 천풍과 자경종은 서로 비슷하였다.

To selection of optimal shading material, two-year-old ginseng (Panax ginseng C. A. Meyer) of new cultivar, 'Cheonpoong' (CP), and native species 'Hwangsookjong' (HS) were cultured under three kinds of shading materials such as three-layered blue and a one-layered black PE (polyethylene) net (TBSB), blue PE sheet (BS), and aluminium coated PE sheet (AS) in imperfectly drained paddy soil. Growth characteristics, yield and ginsenoside contents were investigated under three shading materials. Yield and ginsenoside contents of ginseng were distinctly affected by intensity and quality of sunlight penetrated through shading materials. Light transmission ratio, air and soil temperature according to shading materials were higher in order of BS, AS, and TBSB. However, ratio of aerial phase and porosity of the soil were higher in order of AS, BS, and TBSB, respectively. There was no significantly difference in the ratio of rusty colored root by shading materials. CP showed higher stem length, leaf area, and root weight than that of HS, while the former showed distinctly lower discolored leaf ratio than that the other. Eight kinds of ginsenosides content of CP were higher than that of HS in Rg1, Re, Rf, Rb1 and Rc except Rg2, Rb2, and Rb3. Total ginsenoside contents of CP was distinctly higher than that of HS. Total ginsenoside contents as affected by shading materials was higher in order of BS, TBSB, and AS in CP, while TBSB, BS, and AS in HS.

인삼 연작장해 원인과 경감대책을 수립하고자 2004년에는 2005년까지 작물과학원 증평시험지 연작토양에서 인삼 수확경 과년수와 예정지관리 유무에 따른 시험을 수행한 결과를 요약하면 다음과 같다. 인삼 연작지 수확경과년수가 짧을수록 생존율이 낮아지며 3~4년 경과구 81~95%의 뿌리썩음 이병주율을 보였다. 예정지 관리구는 미관리구보다 생존율이 다소 높으며 뿌리 썩음병 피해도 예정지 미관리와 같은 경향으로 예정지 관리효과는 크지 않다. 수확경과년수가 10년이상의 연작지에서도 연작장해를 일으키는 병원균 (C. destructans)이 분리되므로 재작 또는 연작시는 이점을 고려하여 재배지를 재선정하거나 예정지 토양관리대책을 수립해야 할 것으로 판단된다.

해가림의 이랑방향이 인삼의 생육에 미치는 영향을 구명하고자 방위각 90˚와 270˚ (이랑방향 90˚), 120˚와 300˚ (이랑방향 120˚), 0˚와 180˚ (이랑방향 180˚)를 가로지르는 방향으로 이랑을 만들고 2, 3년생 인삼의 생육특성 및 수량성을 표준재배법의 이랑방향 120˚와 비교한 결과는 다음과 같다. 1. 이랑방향 90˚는 대조구인 120˚에 비해 오전에 투광량의 감소로 해가림 내 기온은 약간 낮았으나 15시 이후에는 투광량의 증가로 대조구보다 높은 기온을 보였다. 2. 이랑방향 180˚는 대조구인 120˚에 비해 9:00~11:00 사이에 투광량의 현저한 증가로 해가림 내 기온이 매우 높았으나 13시 이후에는 대조구와 투광량이 비슷하여 기온도 큰 차이가 없었다. 3. 이랑방향 90˚와 180˚에서 경장, 엽장 및 엽폭은 대조구인 120˚보다 모두 감소되었는데, 90˚보다 180˚에서의 감소가 현저하였다. 4. 엽소율(葉燒率)은 이랑방향 90˚에서 가장 작고 직사광선의 유입이 많았던 180˚에서 가장 컸으며 해에 따라 엽소율(葉燒率)은 큰 변이를 보였다. 5. 2004년과 같이 한발이 심한 해에는 이랑방향 180˚의 수량성이 대조구인 120˚보다 낮았으나 상대적으로 한발이 적은 2005년도에는 180˚가 대조구보다 높은 수량을 보였다.

금산 및 음성지역의 4년생 인삼재배 농가포장에서 직파재배 5개소와 이식재배 5개소를 임의로 선정하여 직파와 이식재배에 따른 생육특성 및 엑스와 조사포닌 함량을 비교한 결과는 다음과 같다. 직파재배는 4년근 생존율이 평균 48%로 이식재배의 86%보다 떨어지나 입모수가 평균 96주/3.3m2순로 이식재배의 57주보다 많고 엽면적지수가 커 수량성이 높은 반면, 주당근중은 작았다. 직파재배는 이식재배에 비해 동체의 신장이 양호하나 지근의 발달이 불량하여 동체중의 비율이 높고 지근중의 비율이 낮았으며, 직파재배는 적변 발생율이 적으나 동체와 지근부위의 엑스와 조사포닌 함량이 낮았다.

경기도 안성지역의 4~6년생 재래종 (자경종) 인삼포장에서 4월 28일에서 9월 30일까지 월별 지하부 생육특성과 엑스함량의 경시적 변화를 조사한 결과는 다음과 같다. 1. 동체장은 월별 수축과 회복을 반복하여 9월 수확기까지 증가되지 않았으나 동체직경은 점진적인 증가를 보였다. 2. 단위 면적당 지상부중은 4월부터 급격히 증가하여 7월에 최고를 보인 후 차차 감소되었으며, 지하부중은 5월에 감소된 후 9월 수확기까지 점차 증가되었는데, 6년생은 7월 이후 약간의 감소를 보였다. 3. 지하부의 상대생장율은 전엽기인 5월과 고온기인 8월에 감소되었고 6, 7, 9월에 다시 증가되었는데, 6년생의 지근과 세근은 4~5년생에 비해 월별 뚜렷한 변화를 보이지 않았다. 4. 상품비율 (근중 60g 이상)은 연생이 증가할수록 커서 4년생 23%, 5년생 60%, 6년생 69% 이었다. 5. 뿌리의 수분함량은 수확기까지 차차 감소되었는데, 7~9월까지 유의적인 차이를 보이지 않았으며, 9월 수확기의 수분함량은 4년생 〉 5년생 〉 6년생순 이었다. 6. 동체의 경도는 5월에 감소된 후 급격히 증가되었는데, 4~5년생은 6~9월까지 대체로 증가되었으나 6년생은 8월부터 차차 감소되어 4~5년생과 유의적인 차이를 보였다. 7. 동체와 지근의 엑스함량은 4월부터 6월까지 급격히 감소된 후 9월에 다시 증가되었는데, 4~5월의 엑스함량은 4년생이 5~6년생보다 많았으나 9월에는 연생 간에 뚜렷한 차이가 없었다.