We developed new two-rowed covered barley ‘Gwangmaeg’ superior to ‘Hopum’ that has been widely cultivated in Korea for using beer-materials. ‘Gwanngmaeg’ was related from the line named as ‘Iksan149’ in 2010 after yield trials test in conducted for three years from 2008. And then it was registered to ‘Plant protection right, No. 5847’ in 2016 after field experiment by KSVS(Korea Seed & Variety Service). ‘Gwangmaeg’ has distinct characteristics in winter type (IV) and mid-tall culm length which makes reduce damage by off-season heading and lodging comparing to those of ‘Hopum’. ‘Gwangmaeg’ have characteristics of cold tolerance and disease resistance to BaYMV(Barley yellow mosaic virus) and Net blotch(Pyrenophora teres). Yield of ‘Gwangmaeg’ was about 611kg/10a and 524kg/10a in upland and paddy field condition, respectively, which higher 2-4% than ‘Hopum’, however, it has no significant difference. In quality examination for beer usage, ‘Gwangmaeg’ has lager grain and higher assortment ratio than those of ‘Hopum’. In malt quality, ‘Gwangmaeg’ showed higher and better quality in malt extract rate and diastatic power than those of ‘Hopum’. ‘Gwangmaeg’ would be suitable for the area above –4℃ of daily minimum temperature in January in Korean peninsula.

To diversify the grain size and shape of japonica rice, we developed the Breeding Materials with Diverse Grain Size and Shape (BM_DGS) and characterized the grain and yield-related traits. We used the donor parents Jizi1560 and Jizi1581, japonica germplasm with extremely large grain size. Four cross combinations between the each donor parents and Korean high yielding japonica rice cultivars, Deuraechan and Boramchan, were constructed and anther culture method was applied. Among 290 doubled haploid lines, we selected 91 elite lines with diverse grain size and shape and designated to the BM_DGS. The grain size and shape of BM_DGS exhibited beyond the characteristics of previously developed Korean rice cultivars. The alleles of major grain-related genes, GW2, GS3, and qSW5, in BM_DGS showed two types, wild type or loss-of-function mutant type. The loss-of-function mutant alleles, gw2, gs3, and qsw5, had an effect on increasing grain size. The phenotypic variation of grain length was mostly controlled by GS3 alleles, and grain width and thickness were influenced by the combinations of GW2 and qSW5 alleles. 1,000-grain weight was determined by the combinations of GW2, GS3, and qSW5. The grain-related genes influenced the phenotypic variation of yield-related traits. The result of this study could be useful to elucidate the relationship between the grain-related genes and agronomic traits. And the BM_DGS are being utilized in the breeding programs to diversify the grain size and shape in japonica rice.

‘Manbaek’ is a bacterial blight resistant mid-late maturing rice cultivar with high grain quality. ‘Manbeak’ was derived from anther culture using the backcross combination, Hopum*2/SR30075. ‘Hopum’ is a mid-late maturing rice cultivar with high grain quality and ‘SR30075’ is a pyramid line carrying three bacterial blight resistance genes. ‘Manbaek’ was selected through the pedigree method, yield trials, and local adaptability tests. ‘Manbeak’ carrying two bacterial blight resistance gene Xa3 and xa5 showed high-level and broad-spectrum resistance against bacterial blight. ‘Manbaek’ was resistant to K3a, mostly virulent race in Korea, and exhibited resistance reaction against 16 Korean bacterial blight isolates. ‘Manbaek’ was a mid-late maturing rice. The heading date of ‘Manbaek’ was August 19th, which was 5 days later than that of ‘Nampyeong’. Manbaek’ was a lodging-tolerant rice with short culm and dark green leaf. Due to the low viviparous germination, ‘Manbaek’ could be a useful material to prevent pre-harvest sprouting. ‘Mabeak’ was resistant to bacterial blight and rice stripe virus, but susceptible to other virus diseases and insect pests. The yield of ‘Manbaek’ was similar to ‘Nampyeong’. ‘Manbaek’ showed excellent grain appearance and good tastes of cooked rice, so that it could contribute to improving the quality of bacterial blight resistant cultivars. ‘Manbaek’, bacterial blight resistant cultivar with high grain quality, is suitable for the cultivation at bacterial blight prone area and has been utilized in the breeding programs for enhancing the resistance against bacterial blight (Registration No. 6069).

Eleven RAPD primers were assessed to analyze genetic diversity of Korean wheat varieties and to develop DNA marker for cultivar identification. The average of the number of polymorphic bands was 5.2 and PIC values showed 0.48, respectively. Ten major clades were presented by phylogenetic analysis. Three cultivars containing Uri, Hanbeak and Jonong were distinct from the others in the phylogenetic dendrogram. Seven cultivar-specific fragments were detected from 11 RAPD fingerprinting among 35 wheat cultivars and they were sequenced. Four Korean wheat cultivars, Eunpa, Jopoom, Yeonbaek and Jeokjoong, were identified newly by four markers, 84, 173, 174 and KWSM011. We convince that these new DNA markers are useful for cultivar fingerprinting and are applied to marker-assisted selection in wheat breeding program.

Wheat is one of major crop and wheat flour is used to various end-use products such as bread, cookies and noodles due to its unique characteristic of it as elasticity and viscosity. Wheat consumption has been generally increasing in not only US and Europe but also Korea. Nevertheless, gluten proteins in wheat endosperm are cause of allergy by food ingestion. Hence, studies on the allergy have been conducting and have been attracting public attention. Herein, we report studies on research trend of the issue with research papers over the last decade for suggestion of future research direction. Since 2012, studies on allergy of gluten proteins have been dramatically increased based on the number of published papers related to the issue. As results of research activities by country, the Europe accounted for 60% corresponding to the number of papers. Next US (13%), Japan (9%), China (5%), and Korea (2%) came. In Korea, studies on allergy of gluten protein are less studied. In the field of studying of gluten allergy, there are two major technologies as preclinical technology and genome research accounted for 58% and 26%, respectively. In Korea, the study on glutenin proteins which is closely related to wheat allergy is actively performed. Therefore, it can be expected that research will be become more active.

This study aimed to determine the optimal harvesting time for covered barley to make grain silage, in Honam region of Korea. We harvested six varieties of barley every third day from 24 to 42 days after heading (DAH). The moisture content decreased from 62.4% at 24 DAH to 24% at 42 DAH. The moisture content at 36 DAH was 44.3%; however, moisture content at 39 and 42 DAH was lower than 40%. Yield of covered barley significantly increased from 24 to 42 DAH (p < 0.05). Yield at 36 DAH (557 kg/10a) was not significantly different from that at 39 and 42 DAH (p < 0.05). With respect to the feed value of barley grain silage, the amount of crude fiber and crude ash was different by harvesting time (p < 0.05). However, the amount of crude protein, crude fat, and total digestible nutrients (TDN) from 24 and 42 DAH was not significantly different. The pH of grain silage from 24 to 42 DAH was between 3.8 and 4.2 and it was stable until 36 DAH (p < 0.05). However, the pH of grain silage at 39 and 42 DAH was 5.2 and 5.8, respectively, which was higher than the pH of silage with good fermentation quality. The lactic acid content of barley grain silage from 24 to 42 DAH decreased from 5.5% to 0.5% (p < 0.05). The amount of lactic acid at 36 DAH was higher than that at 39 and 42 DAH (p < 0.05). With respect to moisture content, yield, feed value, and fermentation, the optimal harvesting time for grain silage of covered barley was 36 DAH. This could increase the use efficiency of harvesting machine for barley and reduce the harvesting time gap between whole barley silage and grain silage in Korea. Moreover, using barley grain silage for animal feed could reduce the import of corn.

Barley breeding program of RDA (Rural Development Administration) has been develop to new hulled barley variety that has been used for malt and beer material. Through this program, we developed new two-rowed covered barley, Imaeg, with disease resistant, lodging tolerant and good malt quality for beer and Korean traditional drink ‘Sikhye’ in 2013. Imaeg showed resistant to Barley yellow mosaic virus (BaYMV), lodging tolerant and early maturing. The regional yield trials were conducted for Imaeg as a breeding line ‘Iksan166’ during three years at four different regions. And then it was registered to Plant protection right, No. 5681 in 2015 after field experiment by KSVS (Korea Seed & Variety Service). Imaeg has I of vernalization and shorter stem length as 77cm than 7cm to control variety Hopum. Yield potential of Imaeg about 4.8ton/ha that higher about 3% in paddy field condition than control, however, it has no significant difference. In quality examination for beer usage, Imaeg has higher 1,000 grain weight (45.6g) and higher assortment ratio (93.6%) than those of Hopum as 42.6g and 90.4% respectively. In malt quality, Imaeg showed higher soluble protein, Kolbach index and diastatic power than those of Hopum significantly. In addition, Imaeg has better ‘Sikhye’ malt quality significantly in lightness of grain (58.4 L value) and growth rate (216.8%) than those of Hopum as 54.3 (L value) and 177.5% respectively. Imaeg would be suitable for cultivation at the area above -4°C of daily minimum temperature in January in Korean peninsula.

Early maturing glutinous rice lines with giant embryo were developed using anther culture. Deuraechan, mid-late maturing high-yielding japonica rice variety with resistance against rice stipe virus (RSV), bacterial blight (BB), and lodging, and Chenghyangna ge, early maturing glutinous rice germplasm with giant embryo were used the parents. F2 seeds from the cross between Deuraechan and Chenghyangna ge with glutinous endosperm and giant embryo were selected and propagated to F2 population. In F2 population, anther culture was conducted using the panicles from the early maturing plants. All doubled haploid (DH) lines showed early maturing, glutinous endosperm, and giant embryo phenotype. Through marker-assisted selections to Stvb-i and Xa3, 17 DH lines carrying both resistance genes were selected. Among 17 DH lines, six lines with more embryo size and better agronomic traits were selected and analyzed their characteristics. These lines were early maturing glutinous rice with giant embryo and showed enhanced yield, resistance against RSV and BB, and lodging, compared to previously developed giant embryo rice varieties. But they were vulnerable to preharvest sprouting which is important trait in early maturing rice. According to the texture and rapid viscosity analysis, DH lines were considered to have appropriate properties of cooked brown rice. They showed less hardness, gummniess, chewiness, and setback. Developed DH lines could be useful materials for diversification of cropping system and enhancing the brown rice consumption but the breeding efforts to improve the vulnerability against preharvest sprouting is required to apply for practical variety.

The 'Misomi' is a rice variety derived from a cross between ‘Sobi’ which has adaptability of low fertilizer cultivation and SR21110 (Ilpum/Jinbu19) by the rice breeding team at National Institute of Crop Science, RDA in 2013. The heading date of ‘Misomi’ is August 14 and six days later than check variety, ‘Hwaseong’. It has 83 cm of culm length and 121 spikelets per panicle. It showed weaker cold tolerance than that of ‘Hwaseong’ during exposed to cold stress. ‘Misomi’ showed resistance to blast disease, K1, K2 and K3 race of bacterial blight and stripe virus, but susceptibility to K3a race, other viruses and planthoppers. The milled rice of this variety exhibits translucent, clear non-glutinous endosperm and short grain shape. It has low protein content (6.2%) and similar amylose content (19.4%) to that of ‘Hwaseong’. ‘Misomi’ showed better palatability index of cooked rice than that of ‘Hwaseong’. Particulary, ‘Misomi’ showed excellent processing adaptability than ‘Chucheong’ and ‘Hwaseong’ on palatability index of cold and aseptic cooked rice. Its milled rice recovery rate is similar to those of ‘Hwaseong’. However, whole grain rate of milled rice is higher than that of ‘Hwaseong’. 'Misomi' has 5.68 MT/ha of milled rice productivity in ordinary cultivation, which was 12% higher than that of ‘Hwaseong’. 'Misomi' could be adaptable to the middle plain area and Midwest coast area in Korea (Grant No. 5635).

‘Jungmo1038', a mid-late maturing, high dry matter yielding rice (Oryza sativa L.) cultivar with glabrous leaf and hull, was developed for whole crop silage (WCS) use. It was derived from a cross between ‘SR24592-HB2319’ with high biomass, smooth leaf and hull and good germinability in low temperature and new plant type (NPT), ‘IR73165-B-6-1-1‘ which had low tillering trait, large panicle, dark green leaf, thick and sturdy stem and vigorous root system. This cultivar had about 125 days growth period from seeding to heading, 99㎝ culm length, 20㎝ panicle length, 13 panicles per hill, 119 spikelets per panicle and 1,000-grain weight of 23.3 g as brown rice in central plain region, Suwon. This hairless WCS rice variety was weak to cold stresses similar to ‘Nokyang’ but was resistant to lodging in the field, strong to viviparous germination and good to low temperature germination. In addition, ‘Jungmo1038’ was resistant to leaf and neck blast but susceptible to bacterial blight, rice stripe virus and brown planthopper. Its average dry matter yield for three years reached 17.2 MT/ha, 21% higher than that of ‘Nokyang’. This glabrous cultivar had 6.6% crude protein and 62.4% total digestible nutrients a little low compared to ‘Nokyang’. In Korea peninsular, 'Jungmo1038' grows well in central and southern plain and is good to harvest between 15 to 30 days after heading to improve its feeding value and digestion rate of livestock(Grant No. 5634).