도로협곡에서의 수목식재에 따른 보도 내 교통유발 대기오염도 변화를 살펴보기 위해 오픈소스 전산유체역학 코드 FDS(Fire Dynamics Simulator)를 사용하여 수목 투과지수, 식재 위치 및 유형에 따른 영향을 조사하였다. 광범위한 시뮬레이션을 통해 1) 관목층 만으로만 구성된 사례, 2) 관목층과 아교목층으로 구성된 사례 및 3) 관목층과 교목층으로 구성된 사례를 포함하는 400개의 시나리오 사례 중 399개 사례에 대해 유효한 데이터를 생성하였다. 분석결과, 보도 위에서 관찰되는 평균화된 정상상태 대기오염 농도는 식재유 형과는 무관하게 수목식재 후 약 10% 이상 감소하는 것으로 나타났다. 가장 큰 대기오염 감소효과는 관목층만으로 구성된 사례에서 관찰되었는데 대략 45%까지 감소하는 것으로 나타났다. 이러한 감소효과는 차선에서 인도로 대기오염물질을 직접 수송하는 선회류가 관목층에 의해 유리한 방향으로 변형되는 물리적 과정에 기초한다.

Aster koraiensis Nakai (A. koraiensis) which has been used as a food and medicinal plant in the past, is valuable as functional food material. Therefore, the aim of this study was to determine the antioxidant properties and major phenolics of A. koraiensis extracts with different ethanol concentrations (0, 50, 70, and 100% aqueous ethanol solution). When ethanol concentration in the extraction solvent was increased, extraction yield decreased; 34.2, 23.2, 21.0, and 5.5% in 0, 50, 70, and 100% ethanolic extracts, respectively. Total phenolics content and antioxidant activities of extracts were increased in an ethanol concentration-dependant manner. The major phenolics in the extracts were chlorogenic acid (21.264~58.666 mg/g), isochlorogenic acid A (10.432~145.353 mg/g), and isochlorogenic acid C(0.239~13.148 mg/g), and these phenolic contents were higher in 70 and 100% ethanolic extracts than other extracts. Significant correlations were observed between ethanol concentration of extraction solvent, antioxidant properties, and major phenolics. These results indicated that the optimal ethanol concentration for extraction was 70%.

To increase the utilization of Centella asiatica (CA), enzymes such as cellulase and pectinase were added and the physicochemical properties of the treated CA were analyzed. In addition, apple-CA jam was prepared using the enzyme-treated CA, which had the best antioxidant properties, and the physicochemical and sensory qualities of the jam were measured. There was a high content of ascorbic acid, polyphenols, flavonoids, reducing sugar, amino acid, minerals and DPPH radical scavenging activity in the enzyme-treated group. The antioxidant component and activity in the jam prepared by adding enzyme-treated CA increased with an increase in the amount of enzyme-treated CA. In the soluble solids, the higher the amount of enzyme-treated CA, the higher the value, but there was no significant difference in pH. The sensory evaluation of the jam, in particular the taste, showed that the highest preference was observed when the enzyme-treated CA was added in the range of 5.0~6.7%, and the control group showed the lowest preference. There was no significant difference in flavor and spreadability among the treatment groups, however, the control group showed the highest color preference. In the overall acceptability, when 5.0% of enzyme-treated CA was added, the highest acceptability was shown.

본 연구는 십자화과 채소인 갓(Brassica juncea) 추출물 을 이용하여 지표성분인 sinigrin의 함량을 분석하고, 내분비계 교란물질 환경호르몬인 비스페놀 A (BPA)로 분화를 유도한 3T3-L1 전구지방세포에서 갓 추출물과 sinigrin 처 리에 대한 지방세포 분화 및 활성산소종(ROS) 생성 억제, 지방 생성 전사인자(PPARγ, C/EBPα, aP2)의 단백질 발현 감소 효능을 평가하였다. 연구 결과에 따르면 HPLC를 이용하여 측정한 갓 추출물 중 sinigrin의 함량은 21.27±0.2 mg/g 인 것으로 나타났다. BPA로 유도된 3T3-L1 전구지방세포에 서 XTT assay 결과 sinigrin 180 μM 및 갓 추출물 300 μg/ mL 농도에서 세포 독성을 보이지 않았으며, 지방세포 분화과정 중 세포 내 지방 축적량과 ROS 생성량을 비교하였을 때 갓과 sinigrin을 처리한 지방세포의 경우 지방축 적량 및 ROS 생성량 모두 유의적으로 감소시키는 것으로 나타났다. 또한, 갓 추출물 및 sinigrin을 처리하였을 때 지 방세포 분화를 조절하는 전사인자 PPARγ, C/EBPα 및 aP2 의 발현이 억제됨을 확인하였다. 이 결과를 통해 갓은 환 경호르몬 비스페놀 A로 유도된 지방세포 내 지방 축적 억제와 더불어 ROS 저감에 효과적으로 작용함을 확인하였다. 향후 sinigrin을 함유한 갓은 BPA로 인한 지질 대사 장애를 예방하는 천연물 유래 기능성 식품 소재로 활용할 가능성이 높은 것으로 기대되며, 추가로 임상 연구 및 작용기전 입증을 위한 in vivo 모델에서의 후속 연구가 진 행되어야 할 것으로 사료된다.

MMBR system has been suggested as a promising system to resolve harvesting problems induced from low settling efficiency of microalgae. And recently, a lot of research on reducing fouling at the MMBR system has investigated focused on EPS in many cases. EPS of microalgae mainly consists of polysaccharides and protein components, and is produced through photosynthesis and nitrogen-carbon metabolic pathways. Especially, P-EPS is one of major compounds which occur membrane fouling phenomenon, as its hydrophobic protein components cause floc formation and cake layer accumulation. And it is already known that almost every microalgae can metabolize P-EPS or Chl-a when nitrogen sources as a substrate is insufficient or exhausted situation. With the above backgrounds, uptake rates of P-EPS or Chl-a by Scenedesmus quadricauda according to the type of carbon source and nitrogen concentration were evaluated in order to verify correlation between carbon source vs P-EPS production, and indeed Scenedesmus quadricauda uses P-EPS or Chl-a when the amounts of nitrogen sourc es in the feed is not satisfied. As a result, it was shown that P-EPS and Chl-a production were increased proportional to nitrogen concentration under organic carbon condition. And especially, the amo unts of P-EPS and Chl-a in the cell were diminished with the nitrogen source becomes insufficient or exhausted. Because P-EPS accelerates fouling at the MMBR system, P-EPS degradation by Scenedesmus quadricauda in order to get nitrogen source may contribute to reducing fouling. About a affects of N-consumed Chl-a to the MMBR fouling, more survey is needed. On the contrary, considering the purpose of MMBR system of this study, i.e. harvesting useful high value microalgae efficiently feeding adequate industrial process wastewater, it seems like difficult to maintain satisfied metabolic activity and to harvest with high yield rate using nitrogen-poor MMBR feed.

In general, trans-membrane pressure (TMP), flux, filtration resistance are used as indicators to evaluate the degree of fouling in MBR. However, they have limitations in determining the level of EPS generation, which is known as an important factor of fouling. Therefore, a new evaluation method is required to monitor the amount of EPS generation. In this study, the applicability of capillary suction time (CST), which is used to measure the dewaterability of sludge, was evaluated as an indirect fouling evaluation index. Statistical analysis was performed to evaluate the effect of EPS on CST, and to determine whether EPS has high similarity with representative fouling evaluation indicators and CST, and quantitatively compared them. As a result, the correlation coefficient between CST and bEPS was 0.7988, which was higher than the correlation coefficient between filtration resistance and bEPS. Since bEPS is a major factor inducing fouling by affecting the formation of the cake layer, it was evaluated that CST, which has a high correlation with bEPS, is suitable to represent EPS. In addition, it was evaluated that the correlation coefficient between filtration resistance and CST was high as 0.7187, which could be used as a fouling evaluation index.

배스(Micropterus salmoides)는 수생태계에서 최상위단계에 위치하는 생태계교란 어종으로 심각한 담수생태계의 불균형을 초래하고 있다. 배스의 퇴치 및 관리를 위한 다양한 시도를 하고 있지만 효과적인 방안은 없는 상황이므로 배스의 고유한 특성에 기반한 개체군 감소의 효율성을 극대화할 수 있는 방식을 모색하였다. 본 연구에서는 배스의 Transcriptom 분석으로 Unigene contigs는 182,887개, 그리고 정자-난자 인식 단백질인 IZUMO1과 Zona pellucida sperm-binding protein의 유전자에서 CRISPR/Cas9 system을 적용할 최종 Target sequence는 12종을 산출하였다. 각 Target sequence를 인식할 수 있는 12종의 sgRNA를 합성한 후 후속 연구에 사용할 12종의 Cas9-sgRNA ribonucleoprotein (RNP) complex를 제작하였다. 본 연구에서는 차세대염기서열 분석법으로 정자-난자 인식 단백질을 암호화하는 유전자를 탐색하였고, CRISPR/Cas9 system으로 유전자를 편집하여 번식행동은 하지만 수정란을 형성하지 못하는 생식세포를 생산하는 불임개체를 유도하기 위한 조성물 개발 과정을 확립하였다. 그리고 배스와 동일한 수계에 있는 고유 생물종의 서식에는 영향을 미치지 않는 생태교란종 관리 방안으로서의 유용성을 검증하기 위한 후속 연구의 귀중한 기초 자료를 확보하는데 기여했다고 판단된다.

To utilize Malus pruniforia Borkh. as a functional material, cold-water (CW), hot-water (HW), and 70% ethanol (EtOH) extracts were prepared, and their antioxidant and anti-inflammatory activities were compared. The antioxidant activity of the HW extract evaluated by ABTS and DPPH radical scavenging and FRAP activity was significantly effective. The total polyphenol content of the HW extract was also higher by 15.5±0.7 mg GAE/g extract compared to other extracts. The EtOH extract showed significantly decreased TNF-α (39.8%), IL-6 (65.5%), and NO (34.9%) levels in RAW 264.7 cells compared to the LPS-induced control group. The levels of IL-6 (21.1%) and IL-8 (19.3%) were significantly decreased by treatment of EtOH extract in HaCaT keratinocytes induced with TNF-α and IFN-γ. The UHPLC-MS results indicated that the EtOH extract might have chlorogenic acid and phlorizin as the major compounds. This was validated using HPLC-DAD, which showed that the EtOH extract had higher levels of chlorogenic acid and phlorizin (1,185±58 and 470±10 μg/g extract, respectively). In conclusion, the present study suggested that the anti-inflammatory activity of the EtOH extract was more effective than the CW and HW extracts, and chlorogenic acid and phlorizin could be used as indicator compounds and functional substances.

최근 농업분야에서 정밀농업을 구현하기 위한 다양한 노력들을 기울이고 있다. 정밀농업분야에서 가장 대표적인 연구분야는 영상정보를 이용하여 작물의 생육 및 수량을 예측하는 것이다. 특히, 여러 영상정보 중에서 다중분광 혹은 초분광영상정보를 기반으로 한 작물의 생육 및 수량예측이 가장 활발하게 연구되고 있는 분야이다. 각각의 센싱장비를 통해서 획득된 영상정보는 여러 방법으로 활용할 수 있으며, 가장 보편적으로 활용하고 있는 방법이 다중분광 및 초분광정보를 이용하여 다양한 식생지수로 산출하고 이를 기반으로 작물의 생육을 예측하는 것이다. 식생지수는 식물체내의 광합성색소함량 및 광합성효율과 높은 상관관계를 보이는 파장을 기반으로 산출된 지수이다. 현재까지 약 150종의 식생지수가 알려져 있으나 농업분야에서 주로 이용하고 있는 식생수는 일부에 불과하다. 이런 이유에서 본 논문에서는 다양한 식생지수들 중 주요한 식생지수들을 선별하여 식생지수들의 산출공식, 적용방법과 관련된 내용을 소개하였다.

This study was conducted to help manage total floating bacteria and fungi in the indoor air by studying the characteristics of total floating bacteria and fungi according to the indoor CO2 concentration of daycare centers. The sampling and analysis of samples was based on the indoor air quality process test method, and the result analysis was conducted using the SPSS statistical program to perform correlation and regression analysis. Correlation and regression results show that CO2 and total airborne bacteria showed positive relationships, but airborne mold did not show relevance. In addition, in order to identify factors affecting airborne mold, correlation analysis and regression analysis were performed regarding total airborne bacteria, PM10, PM2.5, HCHO, outdoor mold, I/O ratio, indoor temperature/ humidity, area per classroom and volume. The results showed that the factors affecting airborne mold were I/O ratio, outdoor airborne mold, and total airborne bacteria. Research results show that CO2 and total airborne bacteria can be reduced and controlled by natural ventilation, and in the case of airborne mold, mechanical forced ventilation such as hoods will be necessary due to the introduction of outdoor airborne mold. In addition, it is necessary to consider I/O ratio criteria in order to confirm effective indoor mold contamination, taking into account the effect of outdoor mold inflow.

With the globalization of Korean education and the rise in the average level of education, the number of international students in Korea, especially those from China, is rapidly increasing every year. This study aimed to provide the basic information for healthy dietary education by evaluating the dietary and nutritional status of Chinese students living in Gwangju province of Korea. Data from 468 subjects who met the study participation criteria and who had given their written informed consent were used for analysis. Of the total subjects, 52.4 and 80.1% were non-drinkers and non-smokers, respectively (p<0.01). It was observed that women were more stressed than men when they did not have convenient access to a Chinese food market near their place of residence (p=0.032). The subjects with a high cultural adaptation consumed kimbap (p<0.001) and pizza (p=0.017) more frequently than the subjects with a low cultural adaptation. An analysis of the nutrient quotient revealed that male scores were higher than those of the females with regard to water intake (p=0.035) and exercise level (p=0.021). For Chinese students living in Korea to maintain proper dietary habits, it is necessary to educate them on how to purchase convenience foods and improve their lifestyle, including limiting drinking and smoking. It is also necessary to develop emotional support programs for stress management and cultural adaptation.

본 연구에서는 Aramid FRP와 모서리보강재로 구속한 엔지니어링플라스틱 보강을 통해 기둥의 횡구속 능력을 증대하여 콘크리트 피복 및 압괴 파괴를 지연시킴으로써 휨 항복 후 연성거동을 유도하여 기존 저층 필로티 건축물의 내진 성능을 확보하는 방법을 제시하고 저층 필로티 건축물의 내진취약요인을 분석하여 필요한 전단보강량을 산정, 제안공법의 목표전단보강을 설정하는 것을 목적으로 한다. 엔지니어링플라스틱을 활용한 기둥의 전단보강형상을 제안하고 필로티 구조의 내진취약요인 보강임계점 예측과 예제모델의 구조해석을 통해 제안공법의 목표전단보강량을 검증하였다. 구조해석 결과, 특별지진하중 적용 시, 휨-축력 내력 초과비율이 타 유형군에 비해 낮은 ST-A1(1축편심)을 기준으로 연면적 750m² 이하와 1축 편심 중심-강심편심율 18%이하에 해당될 경우에는 본 논문에서 제안하는 ‘고성능 복합섬유 패널 전단 보강법’을 적용할 수 있는 경계로 제한하고자 한다.

유리섬유 또는 바잘트섬유로 만들어진 고성능 복합섬유 패널은 고강도 보강재이지만, 구조보강을 위해 정사각형 또는 직사각형 구조물에 패널을 부착할 경우에 일체형 거동을 확보하기 위해 모서리 패널이 사용된다. 이러한 모서리 패널을 이용한 복합섬유 패널의 볼트 접합부를 통해 일체형 거동을 확인하기 위해 실험을 진행하였다. 실험변수로 연단거리 비, 측단거리 비, 볼트 배치 형태(엇모배치와 일렬배치) 및 전단면 수가 설정되었다. 강도평가에 대한 실험 결과, 볼트직경에 대한 연단거리비가 4이상 권장된다는 것을 확인하였고 이를 확보할 시 지압에 의한 파괴모드를 확인하였다. 또한, 볼트배치 2종류의 파괴하중은 값이 유사하였다.

신천의 어류 군집 특성과 하천의 건강성을 밝히기 위해 2019년 4월부터 10월까지 조사를 시행하였다. 조사기간 동안 23개 지점에서 채집된 어류는 12과 30종 3,677개체였다. 우점종은 피라미(Zacco platypus, 28.4%), 아우점종은 대륙송사리(Oryzias sinensis, 13.6%), 그 다음으로 참갈겨니(Zacco koreanus, 11.8%), 버들치(Rhynchocypris oxycephalus, 11.7%), 붕어(Carassius auratus, 9.6%), 참붕어(Pseudorasbora parva, 7.9%) 등의 순으로 우세하게 출현하였다. 출현 종 중 한국고유종은 10종(33.3%)이었고, 외래종은 이스라엘잉어(Cyprinus carpio (Islaeli type), 떡붕어(Carassius cuvieri), 배스(Micropterus salmoides), 구피(Poecilia reticulata), 플래티(Xiphophorus maculatus) 5종(16.7%)이었으며, 육봉형 어류는 둑중개(Cottus koreanus)와 밀어(Rhinogobius brunneus) 2종(6.7%), 기후 변화 민감종은 둑중개 1종(3.3%)이 출현하였다. 어류 군집분석 결과, 신천본류와 수동천, 청담천은 우점도는 낮고 다양도는 높았으며, 동두천과 상패천은 우점도는 높고 다양도는 낮았다. 하천건강성은 최상류와 지류인 수동천이 매우 좋음, 좋음이었으나 상류부는 보통, 중·하류부는 나쁨, 매우 나쁨으로 나타났다. 수질도 중류부터 하류까지 대부분 나쁨 또는 매우 나쁨이었고 지류 수동천만 매우 좋음이었다. 따라서 수질이 어류서식에 큰 영향을 미치면서 결국 하천건강성에 큰 영향을 주는 것으로 판단되었다. 연도별 신천의 수질은 점점 좋아지는 경향을 보였으며 외래종의 도입은 매우 부정적인 요인이었다. 신천의 하천건강성을 향상시키기 위해서는 수질개선과 더불어 외래어종의 관리가 요구된다.

Fresh Omija (Schisandra chinensis) has good marketability, but its quality is difficult to maintain during storage and distribution. Freezing and freeze-thawing treatments can be utilized for the quality maintenance and processing of cold press juice. In this study, the color, antioxidant properties, and the major components of soaked liquor from Omija with freeze-thawing treatment were analyzed during the extraction periods. Each of the frozen and freeze-thawed Omija samples was soaked in 35% ethanol, extracted for 15 days, and used for analysis. The frozen and freeze-thawed samples showed a tendency toward better color and higher antioxidant activity and major component levels than the controls, and freeze-thawing was the best. The results of this study showed that freeze-thawing treatment improved the color, antioxidant properties, and level of the major components of Omija soaked liquor, and freeze storage is suitable for making soaked liquor.

Centella asiatica (CA) is a vegetable that has been used for medicinal purposes for a long time, but it is not well known in Korea. In this study, the approximate analysis, physicochemical properties, free sugars, free amino acids and minerals of native and improved CA cultivated in Chungju area were measured. And the antioxidant contents and activities of CA solvent extracts were measured. There was no significant difference between native and improved species. As for the characteristics of CA, it was confirmed that the improved species had a large weight, size and a tough texture. Glucose was detected in native CA, fructose, glucose and sucrose were detected in improved species. A total of 15 free amino acids were detected and the content was slightly higher in improved species than in native species. The mineral content in CA was slightly higher in the improved species, and the detected minerals were Na, P, Ca, K, Mg and Fe. Polyphenols, ascorbic acid and flavonoids in each extraction solvent (water, 70% and 100% ethanol) were all higher in the improved species than in native species. And it was found that the antioxidant activities of ethanol extracts were higher than that of water.

In MBR, extracellular polymeric substance (EPS) is known as an important factor of fouling; soluble EPS (sEPS) affects internal contamination of membrane, and bound EPS (bEPS) affects the formation of the cake layer. The production of EPS changes according to the composition of influent, which affects fouling characteristics. Therefore, in this study, the effects of the F/M ratio on the sEPS concentration, bEPS content, and fouling were evaluated. The effects of F/M ratio on the amount and composition of EPS were confirmed by setting conditions that were very low or higher than the general F/M ratio of MBR, and the fouling occurrence characteristics were evaluated by filtration resistance distribution. As a result, it was found that the sEPS increased significantly with the increase of the F/M ratio. When the substrate was depleted, bEPS content decreased because bEPS was hydrolyzed into BAP and seemed to be used as a substrate. In contrast, when the substrate is sufficient, UAP (utilization-associated products) was rapidly generated in proportion with the consumption of the substrate. UAP has a relatively higher Protein/Carbohydrate ratio (P/C ratio) than BAP, and this means, it has a higher adhesive force to the membrane surface. As a result, UAP seems like causing fouling rather than BAP (biomass-associated products). Therefore, Rf (Resistance of internal contamination) increased rapidly with the increase of UAP, and Rc (Resistance of cake layer) increased with the accumulation of bEPS in proportion, and as a result, the fouling interval was shortened. According to this study, a high F/M ratio leads to an increment in UAP generation and accumulation of bEPS, and by these UAP and bEPS, membrane fouling is promoted.