자생식물은 관상용, 약용, 식량자원으로 활용될 수 있는 잠재력을 지닌 고유 유전자원이다. 돌부추(Allium koreanum H.J. Choi & B.U. Oh)는 우리나라 해안 암반지대에 분포하는 자생식물 중 하나로, 기후변화와 서식지 감 소로 인해 보전 가치가 높은 식물이다. 이번 연구는 온도와 과산화수소가 돌부추의 발아에 미치는 영향을 조사하기 위해 수행되었다. 종자를 무처리(대조군) 또는 1% 과산화수소(H2O2)로 90분간 처리한 종자를 준비해 15, 20, 25°C 로 설정된 식물 생장 챔버에 배치하였다. 그 결과, 파종 23일 후 15°C에서 42%인 발아율이 20°C와 25°C에서 각각 18%와 0%인 발아율보다 2배 이상 높았으며, H2O2 처리 여부와 관계없이 15°C에서 발아율이 42%로 가장 높았다. H2O2 처리와 관계없이 최종 발아율 50%(T50)에 도달하는 일수는 20°C에서 가장 짧았지만, 일평균 발아율(MDG)은 15°C에서 가장 높았다. 따라서 1%의 H2O2 처리는 돌부추의 발아율에 큰 영향을 미치지 않았으며, 15°C의 온도가 돌부추의 발아율을 높이는 데 최적인 것으로 판단된다. 본 연구 결과는 돌부추의 발아를 위한 기초 연구 자료로 활 용될 수 있을 것으로 기대된다.

뿌리응애류는 양파, 마늘, 생강, 백합 등의 뿌리를 가해한다. 최근 백합재배지에서는 질경이모자이크바이러 스(Plantago asiatica mosaic virus, PLAMV)에 의한 잎의 괴사 피해가 확산되고 있다. 태안 백합재배지에서 PlAMV 가 감염된 백합을 채집하여 구근을 조사한 결과, 식물체 당 뿌리응애 100개체 이상이 발견되었으며 Rhizoglyphus robini로 동정되었다. 이 종이 PlAMV의 보독여부를 확인하기 위해 채집된 R. robini에서 RNA를 추출하여 RT-PCR로 진단한 결과, 모든 개체에서 PlAMV가 확인되었다. 본 연구는 뿌리응애가 백합 구근 뿌리를 먹으면서 만든 상처를 통해 PlAMV가 전염될 수 있다는 가능성을 제시한다.

Wolsong unit 1, the first PHWR (Pressurized Heavy Water Reactor) in Korea, was permanent shut down in 2019. In Korea, according to the Nuclear Safety Act, the FDP (Final Decommissioning Plan) must be submitted within 5 years of permanent shutdown. According to NSSC Notice, the types, volumes, and radioactivity of solid radioactive wastes should be included in FDP chapter 9, Radioactive Waste Management, Therefore, in this study, activation assessment and waste classification of the End shield, which is a major activation component, were conducted. MCNP and ORIGEN-S computer codes were used for the activation assessment of the End shield. Radioactive waste levels were classified according to the cooling period of 0 to 20 years in consideration of the actual start of decommissioning. The End shield consists of Lattice tube, Shielding ball, Sleeve insert, Calandria tube shielding sleeve, and Embedment Ring. Among the components composed for each fuel channel, the neutron flux was calculated for the components whose level was not predicted by preliminary activation assessment, by dividing them into three channel regions: central channel, inter channel, and outer channel. In the case of the shielding ball, the neutron flux was calculated in the area up to 10 cm close to the core and other parts to check the decrease in neutron flux with the distance from the core. The neutron flux calculations showed that the highest neutron flux was calculated at the Sleeve insert, the component closest to the fuel channel. It was found that the neutron flux decreased by about 1/10 to 1/20 as the distance from the core increased by 20 cm. The outer channel was found to have about 30% of the neutron flux of the center channel. It was found that no change in radioactive waste level due to decay occurred during the 0 to 20 years cooling period. In this study, activation assessment and waste classification of End Shield in Wolsong unit 1 was conducted. The results of this study can be used as a basis for the preparation of the FDP for the Wolsong unit 1.

Functional dyspepsia (FD) is a gastrointestinal disorder with diverse symptoms but no structural or organic manifestations. Benachio-F® (herein named ‘BF-1’) is an over-the-counter liquid digestive formulated with multiple herbal extracts, which has been reported to improve symptoms of FD. A total two experiments were conducted. First, we examined whether BF-1 can modulate the progression of FD through two experimental rat models. A total of three doses (0.3x, 1x, 3x of the human equivalent dose) were used. In the gastric emptying model, both 1x (standard) or 3x (3-fold-concentrated) BF-1 enhanced gastric emptying was compared with that of vehicle-treated animals. In a feeding inhibition model induced by acute restraint stress, treatment with 1x or 3x BF-1 led to a similar degree of restoration in food intake that was comparable to that of acotiamide-treated animals. Among the constituents of BF, fennel is known for its choleretic effect. Thus, we next investigated whether a novel BF-based formula (named ‘BF-2’) that contains an increased amount of fennel extract (3.5-fold over BF-1), has greater potency in increasing bile flow. BF-2 showed a superior choleretic effect compared to BF-1. Furthermore, the postprandial concentration of serum secretin was higher in animals pretreated with BF-2 than in those pretreated with BF-1, suggesting that the increased choleretic effect of BF-2 is related to secretin production. Our results demonstrate that BF-1 can modulate the pathophysiological mechanisms of FD by exerting prokinetic and stress-relieving effects, and that BF-2 has a better choleretic effect than BF-1.

본 연구에서는 국내에서 재배되는 스탠다드 절화 장미의 품질 경쟁력을 갖추기 위해 국내에서 재배되어 유통되는 품종과 수입되고 있는 대표적인 품종을 대상으로 외∙내적 품질 비교 분석을 실시하였다. 외적 품질 분석 결과, 국내에서 주로 재배 되는 적색 장미 ‘Fuego’ 은 국내로 수입되고 있는 콜롬비아산 ‘Freedom’, 에티오피아산 ‘Upper class’ 과 비교 시 노화 시점까지 개화가 지속되어 화폭이 증가하고 꽃이 만개하였으며, 노화 발생이 적어 절화수명이 3일 이상 연장되었다. 분홍색 장미 또한 ‘Hera’와 콜롬비아산 ‘Mandela’, 에티오피아산 ‘Visu’ 와 비교 시 수명이 다소 연장되었으며, ‘Fuego’, ‘Hera’ 모두 향기가 다소 진하였다. 내적 품질 분석 결과, 절화수명과 유사 하였으나, 적색과 분홍색 장미 모두 에티오피아산 품종의 수분 흡수량이 다소 높았다. 그러나, 박테리아 발생량은 유의차 가 없었으며 ‘Fuego’는 꽃의 당 함량이 다소 높았다. 또한, 개화단계, 엽록소 함량, 꽃과 잎의 당 함량과 절화수명은 양의 상관관계를 보였다. 따라서, 적색 장미 ‘Fuego’는 수입산 절화 와 비교 시 품질 경쟁력을 갖출 수 있을 것으로 보이나, 수분 흡수를 원활히 하기 위해서는 농가에서 채화하는 직후부터 소비자 판매단계까지의 단계적정 보존용액 처리가 필요할 것으로 판단된다.

Celecoxib, a cyclooxygenase (COX)-2 selective inhibitor, was approved as a non-steroidal anti-inflammatory drug (NSAID), and this therapeutic application has been expanded to several other diseases, including colon cancer. Notably, a treatment strategy combining the use of celecoxib and radiation therapy has been employed for improving the control of local cancers. In this study, we examined the effect of celecoxib on irradiation-induced intestinal damage. The twenty four mice (BALB/c) were divided into four groups; 1) sham-irradiated control group, 2) celecoxib-treated group, 3) irradiated group, and 4) celecoxib-treated irradiation group. Mice were orally administered celecoxib at a dose of 25 mg/kg in a 0.1 mL volume, daily for 4 days after irradiation exposure (10 Gy). Then, histological examinations of the jejunal villous height, crypt survival, and crypt size were performed. The expression of COX-2 after administration of celecoxib in irradiated mice was examined by employing immunohistochemistry, Western blotting, and qPCR analysis. The jejunal villi height and the crypt survival were reduced in the irradiation group compared with the sham-irradiated group. Celecoxib treatment in irradiation mice even more decreased those indicators. Crypt size was increased in the radiation group compared to the sham-irradiated control group, whereas the size was decreased in the celecoxibtreated irradiation group compared with the group exposed to the radiation injury. COX-2 expression was detected in the crypt of the small intestine, and COX-2 expression was increased in the crypt lesion following radiation exposure. However, COX-2 expression was reduced in the celecoxib-treated irradiation group. Therefore, in the present study, we confirmed that celecoxib treatment after irradiation aggravated the irradiation-induced intestinal damage. These results suggest that a caution need to be administered when celecoxib treatment is performed in combination with radiation therapy for cancer treatment.

There is a growing global interest in food choices that could affect health. Consumer food choices are dictated by numerous factors such as attitudes, beliefs, desires and preferences stemming from psychological and physiological attributes. The present study aimed to understand consumers’ internal perceptions about cream soup, cream sauce, and tomato sauce using ZMET (Zaltman’s Metaphor Elicitation Technique). The 9 step ZMET interview process was conducted for a sample of 36 consumers (12 consumers for each sauce). A content analysis of the survey results was carried out. From this, 56 concepts of cream soup were derived, and 15 constructs that met with the agreement of more than one-third of the total sample consumers were extracted. These 15 constructs included ‘Soft’, ‘Recollection’, ‘Familiar’, ‘Warm’, ‘Comfortable’, etc. Similarly, for cream sauce, 67 concepts and 20 constructs ‘Silky’, ‘Warm’, ‘Restaurant’, ‘Family’, ‘Memory’, etc. were deduced. A total of 66 concepts and 20 constructs for tomato sauce ‘Sourness’, ‘Sauce Bottle’, ‘Pleasant’, ‘Ingredient’, ‘Cooking’, etc. were derived. The analysis of consumers’ consensus maps through this study provides a deep and useful understanding of consumers and their latent needs. Also, the results of this study indicate that exploring consumers’ internal perceptions is critical to understanding their healthy food choices. This can be used as basic data for formulating marketing strategies.

본 연구는 스파티필름의 수분 스트레스 정도에 따라 실내 공간 내 오염물질 제거 효율을 구명하고자 수행하였다. 식물이 없는 공간을 대조구, 정상적인 스파티필름과 수분 스트레스를 받은 스파티필름을 각각의 처리구로 하였다. 스파티필름의 수분 스트레스 유무에 따른 chamber 내 온도를 조사한 결과 대조구와 처리구 모두 식물의 생육 적정 범위인 23±1℃를 유지하였으며, 처리 간의 0.7℃의 차이를 보였다. 습도의 경우 대조구와 처리구는 유의차 있게 나타났으며, 처리 간의 유의 차는 없는 것으로 나타났다. 수분 스트레스에 따른 실내 오염 물질을 조사한 결과, 포름알데히드(Formaldehyde) 경우 대조구는 0.30mg･m-3, 정상적인 스파티필름은 0.05mg･m-3 , 수분 스트레스를 받은 스파티필름은 0.09mg･m-3으로 대조구와 처리구는 통계적으로 유의차를 보였으며, 식물 내 수분 스트레스에 따른 처리구간에는 유의차가 없었다. TVOC(Total Volatile Organic Compound)조사 결과, 정상적인 스파티필름의 TVOC는 5시간 후 0.00mg･m-3 으로 모두 제거 된 반면, 수분 스트레스를 받은 스파티필름은 0.34mg･m-3으로 다소 남아 있었으며, 대조구는 1.25mg･m-3으로 세 처리 모두 통계적으로 유의차 있게 나타났다. 또한 이산화탄소 변화량 조사결과, 대조구는 459ppm, 정상 스파티필름은 446ppm으로 통계적으로 유의한 차이는 없으며, 수분 스트레스를 받은 스파티 필름이 대조구보다 이산화탄소 함량이 다소 높았다. 기공변화율 조사 결과, 정상 스파티필름의 변화율은 높게 나타났으며, 수분 스트레스를 받은 스파티필름은 변화율이 낮은 것으로 조사되었다. 따라서, 스파티필름이 배치되어있지 않은 공간보다 배치된 공간이 공기정화에 효과적이며, 수분 스트레스를 받은 스파티필름은 실내오염물질 제거에 있어서 기공 변화율 및 이산화탄소 흡수능력이 저하되므로 스파티필름을 이용하여 효과적으로 실내오염물질을 제거하기 위해서는 적절한 수분 관리가 필요한 것으로 판단된다.

모바일 게임 시장이 스마트폰 중심으로 변화되기 시작하면서 모바일 게임에서는 오토 플레이 시스템이 적용되기 시작하였다. 오토플레이 시스템은 버튼 한번으로 게임을 자동으로 진행하는 시스템 이며 현재 거의 대부분의 모바일 게임에서 적용되었고 PC게임에서 까지 이러한 시스템이 적용되고 있다. 하지만 이러한 오토 플레이 시스템의 성능은 매우 비효율적으로 행동하고 있으며 본 논문에서는 플레이어의 행동 패턴을 기반으로 학습한 인공지능을 제안하고자 한다. 본 논문에서 제안하는 인공지능 모델은 플레이어가 게임을 진행하면서 게임 데이터와 플레이어가 입력한 버튼 값을 학습 데이터로 저장하고 학습데이터를 DNN(Deep Neural Network) 신경망 모델을 사용하여 학습하였다. 게임에서는 플레이어가 중복적으로 다른 버튼을 동시에 누르기 때문에 Output Layer를 다층으로 분류하여 학습을 진행했다. 본 논문 실험에서는 20명의 실험자들에게 제안하는 인공지능 모델을 사용함으로써 결과를 기록하였고 트랙을 일정하게 벽을 부딪치지 않고 달린 플레이어 데이터만 제대로 학습되어 결과를 얻을 수 있었고 그렇지 않은 플레이어의 데이터는 캐릭터가 제대로 이동하지 않아 결과 값을 얻을 수가 없었다. 또한 간단한 아케이드 게임을 만들어 강화학습과 비교하였으며 강화학습보다 성능은 좋지 않았지만 학습속도가 약 10배 빨랐다.

Cryopreservation of bovine embryos is used to efficiently implant surrogate mothers. It has been widely accepted that high lipid content in the oocyte interrupts its survival during freeze-thaw cycles. Serum component in the culture medium is thought to increase the embryo`s lipid contents. Conversely, L-carnitine stimulates lipid metabolism by transporting long chain fatty acids into the mitochondria. Objective of this study was to analyze the effect of L-carnitine supplementation in IVM medium and defined IVC medium on the development, lipid contents and the cryosurvival of bovine IVF embryos. 0.0, 1.5, 3.0 and 6.0 mM L-carnitine was supplemented in IVM medium, respectively (IVM-LC 0.0, LC 1.5, LC 3.0 and LC 6.0). Development rate from the 2cell to the morula stages was higher in IVM-LC 3.0 groups than those of IVM-LC 6.0 (p<0.05). But there were no significant differences among the other groups in the blastocyst rates and lipid content results. When 0.0, 1.5, 3.0 and 6.0 mM L-carnitine were supplemented in IVC medium (IVC-LC 0.0, LC 1.5, LC 3.0 and LC 6.0), development competence was not significantly different between those embryos. Lipid contents of embryos treated L-carnitine (IVC-LC 1.5, 3.0 and 6.0) were significantly lower than embryos of non-treated group. L-carnitine was supplemented 0.0, 1.5, 3.0, 6.0 mM during IVM and 3.0 mM during IVC (LC 0.0 - 3.0, LC 1.5 – 3.0, LC 3.0 – 3.0, LC 6.0 – 3.0) and cryosurvival of blastocysts confirmed after freezing-thawing. There were no significant differences on development, but LC 3.0 – 3.0 was significantly lower lipid contents than other groups. And LC 3.0 – 3.0 had better survival rates and hatched rates of blastocysts than LC 0.0 – 0.0. In conclusion, supplementation of L-carnitine in defined IVC medium decreases lipid contents. And L-carnitine supplementation improves cryosurvival and developmental ability of bovine IVF embryos.

The early-onset familial Alzheimer's disease (EOFAD/ FAD), the less common type of Alzheimer's disease (AD) currently affects a vast number of individuals worldwide. This type is being inherited as an autosomal dominant fashion. Missense mutations on Amyloid precursor protein (APP) and Presenilins 1 and 2 (PSEN1 & PSEN2) are known as major genetic factors in FAD. Conversely, missense mutations on microtubule-associated protein tau (MAPT) are also thought to involve. Up to date, several triple-transgenic animal models with muted forms of the human APP, PSENs and MAPT have been reported. Compared to other animals, canines are more emotional and their disease signs can be easily diagnosed. This attempt was to develop a triple transgenic canine model for the AD. We have obtained the coding sequences of APP, PSEN1 and MAPT from Dana-Farber/Harvard Cancer Center DNA resource core at HMS and incorporated several common AD mutations. The transgenic construct is composed of hNSE (ENO2) promoter-driven three AD genes fused together with modified 2A sequences. It was transfected into the canine fetal fibroblasts which were then used to perform somatic cell nuclear transfer (SCNT). The viable transgenic embryos were obtained after in vitro culture and the GFP was detected. In this study, we have successfully produced viable triple transgenic canine cloned embryos using SCNT technique. These transgenic canine embryos will be further developed into canines with FAD. The transgenic canines will be a good candidate in the AD research field.