To investigate the effect of the catalyst and metal–support interaction on the methane decomposition behavior and physical properties of the produced carbon, catalytic decomposition of methane (CDM) was studied using Ni/SiO2 catalysts with different metal–support interactions (synthesized based on the presence or absence of urea). During catalyst synthesis, the addition of urea led to uniform and stable precipitation of the Ni metal precursor on the SiO2 support to produce Ni-phyllosilicates that enhanced the metal–support interaction. The resulting catalyst upon reduction showed the formation of uniform Ni0 particles (< 10 nm) that were smaller than those of a catalyst prepared using a conventional impregnation method (~ 80 nm). The growth mechanisms of methane-decomposition-derived carbon nanotubes was base growth or tip growth according to the metal–support interaction of the catalysts synthesized with and without urea, respectively. As a result, the catalyst with Ni-phyllosilicates resulting from the addition of urea induced highly dispersed and strongly interacting Ni0 active sites and produced carbon nanotubes with a small and uniform diameter via the base-growth mechanism. Considering the results, such a Ni-phyllosilicate-based catalyst are expected to be suitable for industrial base grown carbon nanotube production and application since as-synthesized carbon nanotubes can be easily harvested and the catalyst can be regenerated without being consumed during carbon nanotube extraction process.

This study examined the subacute oral toxicity of Dendropanax morbiferus H.Lév leaves hot-water extracts (DMWE) using male and female Spargue-Dawley rats. Rats were orally administered the DMWE at dose levels of 0, 250, 500, 1,000, and 2,000 mg/kg body weight (BW) for four weeks. For experimental period, clinical signs and the number of deaths were examined, and feed intake and BW of all experimental animals were measured once a week for four weeks. At the end of the experiment, blood samples were collected from all rats, and all animals were euthanized and autopsies were performed to collect major organs. No dead animals were found during the experimental period. In addition, no differences were found between control and DMWE-treated groups in feed intakes, BW changes, organ weights, clinical signs, hematological parameters, and serum biochemical parameters. The results of this study provided evidence that oral administration of DMWE at the dose of 2,000 mg/kg BW is safe in rats and may not exert severe toxic effects.

In this study, the acute toxicity of Dendropanax morbiferus H.Lév leaf hot-water extracts (DMWE) was examined in male and female ICR mice. Mice were orally administered the DMWE at dose levels of 0, 250, 500, 1,000 and 2,000 mg/kg body weight (BW) for single-dose toxicity test. There were no significant differences in change of BW between control and all DMWE treated-groups. In hematological and blood biochemical analysis, none of the parameters were affected by the DMWE. Similarly, there were no significant effects on markers for liver and kidney functions in all DMWE treated-groups. Since there were no adverse effects of the DMWE in single oral toxicity tests, even at the highest doses, it was concluded that the lethal dose 50 (LD50) of DMWE is estimated at > 2,000 mg/kg BW.

Current studies have revealed the capacity of mesenchymal stem cells (MSCs) in term of immunomodulatory properties, and this distinct potential is downgraded according to the disease duration of patients-derived MSCs. In order to enhance the immunomodulatory and anti-tumorigenic properties of the rheumatoid arthritis (RA) joints-derived MSCs, we aggregate synovial fluid-derived MSCs from RA joints (RA-hMSCs) into 3D-spheroids by the use of hanging drop culture method. Cells were isolated from synovial fluids of RA joints with longstanding active status over 13 years. For aggregation of RA-hMSCs into 3D-spheroids, cells were plated in hanging drops in 30 μL of advanced DMEM (ADMEM) containing 25,000-30,000 cells/ drop and cultured for 48 h. To analyze the comparative immunomodulatory effects of 3D-spheroid and 2D monolayer cultured RA-hMSCs and then cells were cultured in ADMEM supplemented with 20% of synovial fluids of RA patients for 48 h and were evaluated by qRT-PCR for their expression of mRNA levels of inflammatory and antiinflammatory markers. Cellular aggregation of RA-hMSCs was observed and cells were aggregate into a single sphere. Following treatment of RA patient’s synovial fluids into the RA-hMSCs, spheroids formed RA-hMSCs showed significantly (p < 0.05) higher expression of TNFα stimulated gene/protein 6 (TSG-6) than the monolayer cultured RAhMSCs. Therefore, the 3D-spheroid culture methods of RA-hMSCs were more effective than 2D monolayer cultures in suppressing inflammatory response treated with 20% of RA-synovial fluids by expression of TNFα (TSG-6) according to the immune response and enhanced secretion of inflammatory factors.

Pseudo-pheochromocytoma is defined as an adrenal tumor that has the typical characteristics of pheochromocytoma likely hypertension but is histopathologically diagnosed to other types of adrenal tumors. 11-year-old, castrated male Maltese presented for a left adrenal mass with hypertension. According to clinical signs and radiological features, a temporary diagnosis was made of pheochromocytoma. The adrenalectomy was performed and the histopathological examination of the resected adrenal gland was diagnosed as cortical adenocarcinoma. This report describes the case of pseudo-pheochromocytoma in a dog and implies the salience that even if pheochromocytoma is diagnosed based on all test results, pseudo-pheochromocytoma should not be excluded.

Organisms constituting a large proportion of marine ecosystems, ranging from bacteria to fish, exhibit fluorescence and bioluminescence. A variety of marine organisms utilize these biochemically generated light sources for feeding, reproduction, communication, and defense. Since the discovery of green fluorescent protein and the luciferin-luciferase system more than a century ago, numerous studies have been conducted to characterize their function and regulatory mechanism. The unique properties of fluorescent and bioluminescent proteins offer great potential for their use in a broad range of applications. This short review briefly describes the functions and characteristics of fluorescent and bioluminescent proteins, in addition to summarizing the recent status of their applications.

The biological efficiencies of promising Pleurotus spp. were evaluated. Pleurotus ostreatus, Pleurotus tuberregium,and Pleurotus sajor-cajuwere investigated at temperatures of 22oC, 20oC, and 18oC to determine their ability to adapt to temperatures that are likely to be found in subtropical regions. The experiment was conducted using a substrate comprising Popular sawdust 60% + cotton seed 20% + beet pulp 20% under a relative humidity of 65%. The results indicated that there were significant differences in terms of biological efficiency between the species: P. tuber regium 128.84% at 22oC, P. ostreatus 108.41% at 22oC, and P. sajor-caju is 80% at 20oC. The common temperatures at which all species showed the highest biological efficiencies were 22oC and 20oC. Therefore, the production of the evaluated species was equal to or surpassed biological efficiencies reported in tropical environments, thereby demonstrating a potential opportunity to adapt to tropical environments without compromising quality standards.

Gamma spectrometry is one of the main analysis methods used to obtain information about unknown radioactive materials. In gamma-ray energy spectrometry, even for the same gamma-ray spectrum, the analysis results may be slightly different depending on the skill of the analyst. Therefore, it is important to increase the proficiency of the analyst in order to derive accurate analysis results. This paper describes the development of the virtual spectrum simulator program for gamma spectrometry training. This simulator program consists of an instructor module and trainee module program based on an integrated server, in which the instructor transmits a virtual spectrum of arbitrarily specified measurement conditions to the students, allowing each student to submit analysis results. It can reproduce a virtual gamma-ray energy spectrum based on virtual reality and augmented reality technique and includes analysis function for the spectrum, allowing users to experience realistic measurement and analysis online. The virtual gamma-ray energy spectrum DB program manages a database including theoretical data obtained by Monte Carlo simulation and actual measured data, which are the basis for creating a virtual spectrum. The currently developed database contains data on HPGe laboratory measurement as well as in-situ measurements (ground surface, decommissioned facility wall, radiowaste drum) of portable HPGe detectors, LaBr3(Ce) detector and NaI detector. The analysis function can be applied not only to the virtual spectrum, but also to the input measured spectrum. The parameters of the peak analysis algorithm are customizable so that even low-resolution spectra can be properly analyzed. The validity of the database and analysis algorithm was verified by comparing with the results derived by the existing analysis programs. In the future, the application of various in-situ gamma spectrometers will be implemented to improve the profiling of the depth distribution of deposited nuclides through dose rate assessment, and the applicability of the completed simulator in actual in-situ gamma spectrometry will be verified.

Nuclear power plants decommissioning is planned to be started in middle of the 2020. It is necessary to develop safety evaluation and verification technology during decommissioning to ensure the safety of security monitoring measures and maintenance measures, appropriate emergency plans and preparations for decommissioning, and the use of proven engineering when establishing decommissioning plan. For this purpose, a nuclear power plant decommissioning plan is prepared in several stages before decommissioning. When a lifetime of a nuclear power plant has reached, it needs to be decommissioned and therefore operator company should submit decommissioning plans to the National Safety and Security Commission. And safety analysis should be included in this document and it is explained in chapter 6. According to the NSSC Notice No. 2021-10, it is largely divided into principles and standards, exposure scenarios, dose assessment, residual radioactivity, abnormal events, and risk analysis. When unexpected radiological accident is happened, both public and occupational dose analysis should be conducted. However, research on the former can be found easily on the other hands, research on the latter is not active. In this paper, method of choosing scenarios of accidents during the decommissioning the nuclear power plants is briefly introduced. Accidents during nuclear power plants decommissioning cases in USA is chosen and its risk is evaluated by using risk matrix and ranked by AHP method. During the decommissioning phases, varieties of radioactive waste is expected to be generated such as contaminated concrete and metal. On the other hand, Dry Active Waste (DAW) is generated and its amount is and its amount is 7,353 drums. Characteristic of DAW is highly flammable compared to concrete or metal. Moreover, depending on method of radioactive waste conditioning and type of radioactive nuclides, release rate of the nuclides varies. Thus this type of radioactive waste is critical to fire accidents and such accident can occur extra dose exposure which exceeds the guideline of the regulatory body to workers. Therefore, in this paper, occupational dose exposure during the fire accident is conducted.

Radioactive source terms are important factor in design, licensing and operation of SMR (Small Modular Reactor). In this study, regulatory requirements and evaluation methodology for normal operation on NuScale SMR, which received standard design certification approval on September 11, 2020 from US NRC, are reviewed. The radioactive waste management system of nuclear power reactor should be designed to limit radionuclide concentration in effluents and keep radioactive effluents at restricted area boundary ALARA according to 10 CFR 20 and 10 CFR 50 Appendix I. Also, in general, the coolant source term to calculate the off-site radiological consequences for normal operation of SMR should be determined by using models and parameters that are consistent with regulatory guide 1.112, NUREG- 0017 and the guidance provided in ANSI/ANS-18.1-1999, and the result should be corrected by reflecting the design characteristics of SMR. The coolant source term of NuScale, unlike the case of large NPPs, cannot rely solely on empirical source term data, because the NuScale source term is based on first principle physics, operational experience from recent industry, and lessons learned from large PWR operation. Fission products in reactor coolant are conservatively calculated using first principle physics in SCALE Code assuming 60 GWD/MTU. The release of fission products from fuel to primary coolant based on industry operational experience is determined as fuel failure fraction of 0.0066% for normal operation source term and 0.066% for design basis source term while coolant source term of large NPP is calculated by using ANSI/ANS-18.1 for normal operation and fuel failure fraction of 1% for design basis source term. Water activation products in reactor coolant are calculated from first principles physics and corrosion activation products are calculated by utilizing current large PWR operating data (ANSI/ANS 18.1- 1999) and adjusted to NuScale plant parameters. Also, because ANSI/ANS 18.1-1999 is not based on first principle physics models for CRUD generation, buildup, transport, plate-out, or solubility, NuScale has incorporated lessons learned by using ERPI’s primary water chemistry and steam generator guidelines to ensure source term is conservative and design of materials used cobalt reduction philosophy to help ensure the coolant source term are conservative. Based on the coolant source term calculated according to the above-described method, the annual releases of radioactive materials in gaseous and liquid effluents from NuScale reactor are evaluated. Currently, Small Modular Reactors such as ARA, SMART 100 are under review for licensing in Korea. This study will be helpful to understand how the reactor coolant system source terms are defined and evaluated for SMR.

Organic complexing agents may affect the mobility of radionuclides at low- and intermediate-level radioactive waste repositories. Especially, isosaccharinic acid (ISA) is the main cellulose degradation product under high pH conditions in cement pore water. ISA can combine with radionuclides and form stable complexes that adversely influence adsorption in the concrete phase, resulting in radionuclides to leach to the near- and far-fields of repositories. This study focuses on investigating the sorption of ISA onto engineered barriers such as concrete, thereby studying adsorption isotherms of ISA on concrete and comparing various isotherm models with the experimental data. The adsorption experiment was conducted in three background solutions, groundwater (adjusted to pH 13 using NaOH), State 1 (artificial cement pore water, pH 13.3), and State 2 (artificial cement pore water, pH 12.5), in a batch system at a temperature of 20°C. Concrete was characterized using BET, Zeta-potential analyzer, XRD, XRF, and SEM-EDS. ISA concentrations were detected using HPLC. The experimental data were best fitted to one-site Langmuir isotherm; On the other hand, either two-site isotherm or Freundlich isotherm couldn’t give reasonable fitting to the experimental data. The observed ISA sorption behavior on concrete is crucial for the disposal of radioactive waste because it can significantly lower the concentration of ISA in the pore water. Although one-site Langmuir isotherm might effectively represent the sorption behavior of ISA on concrete, the underlying mechanism is still unknown, and further investigation should be done in the near future.