Haemaphysalis longicornis는 사람과 동물에게 여러 심각한 병원체를 전달하는 주요 매개체로, 한반도에 널리 분포하고 있다. H. longicornis는 Rickettsia spp., Borrelia spp., Francisella spp., Coxiella spp., 그리고 중증열성혈소판 감소증후군 바이러스 (SFTS virus) 등을 매개하는 것으로 알려져 있다. 국내에 서식하는 H. longicornis의 미생물 군집과 관련된 연구는 많이 진행되지 않은 것으로 확인되었다. 이 연구는 한반도 내 다양한 지역에서 채집된 H. longicornis의 미생물군집 다양성을 지역별, 성장 단계 및 성별에 따라 분석하였다. 2019년 6월부터 7월까지 질병관리청 권역별기후변화매개체감시거점센터 16개 지역에서 채집한 H. longicornis의 16S rRNA 유전자 V3-V4 영역을 PCR로 증폭 후 Illumina MiSeq 플랫폼으로 시퀀싱하였다. Qiime2를 활용한 미생물 다양성 분석을 통해 총 46개의 샘플에서 1,754,418개의 non-chimeric reads를 얻었으며, 평균 126개 의 operating taxonmic unit (OTU) 을 식별하여 총 1,398개의 OTU를 확인하였다. 대부분의 지역에서 Coxiella spp.가 우점종으로 나타났으며, 특히 Coxiella endosymbiont는 가장 높은 우점도를 보이며, Coxiella burnetii와 계통 발생 학적으로 유사한 것으로 확인되었다. 이 연구를 통해 분석된 결과는 각 지역의 H. longicornis 미생물군집 데이터 베이스 구축에 활용되었으며, 이를 통해 지역별 미생물군집의 특이성을 식별할 수 있게 하였다. 이는 한반도의 H. longicornis에 의한 질병 전파 연구와 이를 통한 공중보건 개선에 기여할 것으로 기대된다.
The purpose of this study is to select various insect species for healing resources and develop a healing program in order to use insect as a healing agriculture. In this study, there are two kinds of breeding kit were developed, one for Gryllus bimaculatus and the other for Oxya chinensis sinuosa. Using these insect breeding kits, we conducted a survey of 60 children and the elderly. In the case of children, the results of the insect breeding satisfaction showed that 30.6% said that the sound of crickets was very good, and 11.1% said that it was good. In addition, the higher the child's awareness of insects, the higher the proportion of children who wanted to raise insects in the future. As a result of a survey of seniors, 45.2% do not like insects and 51.6% are not interested, meaning that most seniors are not very interested in insects. However, the emotions after breeding insects showed positive results, with 45.2% saying their personality became brighter, 48.4% reducing their anger, 48.4% relieving their irritation, 54.8% relieving loneliness, 58.1% feeling more responsible, and 51.6% developing intimacy.
The domestic Pressurized Heavy Water Reactor (PWHR) nuclear power plant, Wolsong Unit 1, was permanently shut down on December 24, 2019. However, research on decommissioning has mainly focused on Pressurized Water Reactors (PWRs), with a notable absence of both domestic and international experience in the decommissioning of PHWRs. If proper business management such as radiation safety and waste is not performed, it can lead to increased business risks and costs in decommissioning. Therefore, the assessment of waste volume and cost, which provide fundamental data for the nuclear decommissioning process, is a crucial technical requirement before initiating the actual decommissioning of Wolsong Unit 1. Decommissioning radiation-contaminated structures and facilities presents significant challenges due to high radiation levels, making it difficult for workers to access these areas. Therefore, technology development should precede decommissioning process assessments and safety evaluations, facilitating the derivation of optimal decommissioning procedures and ensuring worker safety while enhancing the efficiency of decommissioning operations. In this study, we have developed a program to estimate decommissioning waste amounts for PHWRs, building upon prior research on PWR decommissioning projects while accounting for the specific design characteristics of PHWRs. To evaluate the amount of radioactive waste generated during decommissioning, we considered the characteristics of radioactive waste, disposal methods, packaging container specifications, and the criteria for the transfer of radioactive waste to disposal operators. Based on the derived algorithm, we conducted a detailed design and implemented the program. The proposed program is based on 3D modeling of the decommissioning components and the calculation of the Work Difficulty Factor (WDF), which is used to determine the time weighting factors for each task. Program users can select the cutting and packaging conditions for decommissioning components, estimate waste amount based on the chosen decommissioning method, and calculate costs using time weighting factors. It can be applied not only to PHWRs, but also to PWRs and non-nuclear fields, providing a flexible tool for optimizing decommissioning process.
With the aging of nuclear power plants (NPPs) in 37 countries around the world, 207 out of 437 NPPs have been permanently shutdown as of August 2022 according to the IAEA. In Korea, the decommissioning of NPPs is emerging as a challenge due to the permanent shutdown of Kori Unit 1 and Wolsong Unit 1. However, there are no cases of decommissioning activities for Heavy Water Reactor (HWR) such as Wolsong Unit 1 although most of the decommissioning technologies for Light Water Reactor (LWR) such as Kori Unit 1 have been developed and there are cases of overseas decommissioning activities. This study shows the development of a decommissioning waste amount/cost/process linkage program for decommissioning Pressurized Heavy Water Reactor (PHWR), i.e. CANDU NPPs. The proposed program is an integrated management program that can derive optimal processes from an economic and safety perspective when decommissioning PHWR based on 3D modeling of the structures and digital mock-up system that links the characteristic data of PHWR, equipment and construction methods. This program can be used to simulate the nuclear decommissioning activities in a virtual space in three dimensions, and to evaluate the decommissioning operation characteristics, waste amount, cost, and exposure dose to worker. In order to verify the results, our methods for calculating optimal decommissioning quantity, which are closely related to radiological impact on workers and cost reduction during decommissioning, were compared with the methods of the foreign specialized institution (NAGRA). The optimal decommissioning quantity can be calculated by classifying the radioactivity level through MCNP modeling of waste, investigating domestic disposal containers, and selecting cutting sizes, so that costs can be reduced according to the final disposal waste reduction. As the target waste to be decommissioning for comparative study with NAGRA, the calandria in PHWR was modeled using MCNP. For packaging waste container, NAGRA selected three (P2A, P3, MOSAIK), and we selected two (P2A, P3) and compared them. It is intended to develop an integrated management program to derive the optimal process for decommissioning PHWR by linking the optimal decommissioning quantity calculation methodology with the detailed studies on exposure dose to worker, decommissioning order, difficulty of work, and cost evaluation. As a result, it is considered that it can be used not only for PHWR but also for other types of NPPs decommissioning in the future to derive optimal results such as worker safety and cost reduction.
Once a radioactive material is released from the nuclear power plant (NPP) by accident, it is necessary to understand the behavior of radioactive plume to protect residents adequately. For this, it is essential to measure the radiation dose rate around NPPs at important locations. Our previous study developed a movable radiation detector that can be installed quickly in an accident to measure gamma dose rate in areas where environmental radiation monitoring system is not installed. The data measured by the detector are transmitted to the server in real-time through LoRA wireless communications. There are two methods to use LoRA communications; one is self-network, and the other is the network provided by the mobile carrier. A signal receiver, called a gateway, should be equipped near the installation location of radiation detectors to use a self-network without using the mobile carrier’s system. In other words, the movable radiation detectors we made can function if there should be any gateway near them. The distance capable of communication between gateway and detector is about 8 km in an open area without significant obstacles. Korea has many significant obstacles, such as mountains around most NPPs. Thus, the gateways could be installed in the proper position before the accident to operate the movable radiation detectors without problems. If the gateway is located at a high position like a mountain top, it could cover a wide area. In this study, the elevation database in the area around the NPPs was collected and analyzed to determine where gateways should be installed. The analysis range is limited in the urgent protective action planning zone. The optimization was also performed to minimize the number of gateways.
The bacterial soft-rot disease is one of the most critical diseases in vegetables such as Chinese cabbage. The researchers isolated two bacteria (Pseudomonas kribbensis and Pantoea vagans) from diseased tissue samples of Chinese cabbages and confirmed them as being the strains that cause soft-rot disease. Lactic-acid bacteria (LAB), were screened and used to control soft-rot disease bacteria. The researchers tested the treatments with hypochlorous acid water (HAW) and LAB supernatant to control soft-rot disease bacteria. The tests confirmed that treatments with the HAW (over 120 ppm) or LAB (Lactobacillus plantarum PL203) culture supernatants (0.5 mL) completely controlled both P. kribbensis and P. vagans.
Colon cancer has been considered a leading cause of cancer-associated death. Folic acid is a vitamin necessary for cellular physiological functions and cell viability. However, the association between folic acid intake and colon cancer has been examined in several prospective cohort studies are controversial. This study investigated the effects of folate intake on colon carcinogenesis and oxidative stress in an azoxymethane (AOM)/dextran sodium sulfate (DSS) institute for cancer research (ICR) mouse model. Thirty male ICR mice (5 weeks old) were divided into the control group and the experimental group supplied 0.03% folic acid via drinking water (50 mL/week/mouse) for 6 weeks. To induce colonic pre-neoplastic lesions, the animals were subcutaneously injected three times weekly with AOM (10 mg/kg body weight), followed by 2% DSS in drinking water for a week. Folic acid supplementation significantly suppressed the total number of aberrant crypt foci and aberrant crypts. Histological image data showed that folic acid supplementation attenuated neoplastic change. In addition, we measured the thiobarbituric acid reactive substances concentration of dry feces samples to identify the effect of folic acid on reactive oxygen accumulation. The folic acid supplementation group had reduced reactive oxygen species levels in dry feces compared to the control group. In conclusion, these findings indicate that folic acid suppresses colon carcinogenesis and oxidative stress in an AOM/DSS mouse model.
In this study, the effects of heat-moisture treatment (boiling or steaming for 45 min) prior to hot air drying (70 o C for 8 h) on water activity (Aw), moisture (MC), Brix, color, and texture of dried sweet potato slices were investigated to identify the best pretreatment condition for producing dried sweet potato with the best eating quality. Dried sweet potato slices pretreated by boiling (BL) showed significantly lower Aw, MC, and hardness while Brix was significantly higher than with steaming (ST) treatment. There were no significant differences for the color, springiness, cohesiveness, gumminess, and brittleness indexes. At 8 h drying, the Aw, MC, Brix, and hardness of the BL and ST samples were 0.81 and 0.82, 19.71% and 25.53%, 53.80% and 49.40%, and 20.49 kg/cm 2 and 31.98 kg/ cm 2 , respectively. This information will be useful for manufacturers in the production of dried sweet potato slices. These findings provide evidence of the feasibility of heat-moisture treatments in the production of dried sweet potato.
The present study was conducted to evaluate the effects of high levels of nutrients on the growth performance, blood metabolites and carcass characteristics of Hanwoo cattle. Eighteen Hanwoo steers were fed two types of diets: 1) Nine animals were fed the conventional diet including typical levels of crude protein (CP) and total digestive nutrients (TDN), and 2) Nine animals were fed the treatment diet including high levels of CP and TDN. The average body weight (BW) and dry matter intake (DMI) were greater (P < 0.05) in the treatment group than in the conventional group at early and late fattening stages. Also, in the treatment group, the average daily gain (ADG) was greater (P < 0.05) at the late fattening stage. The serum total lipid and cholesterol levels were higher (P < 0.05) in the late fattening stage of treatment group. The carcass weight, total fat weight, longissimus muscle area and the grade of meat quality were also greater (P < 0.05) in the treatment group than the conventional diet group. This study demonstrates that high levels of CP and TDN exhibit a positive effect on the growth performance and carcass characteristics, indicating that high levels of CP and TDN can be used as a cost-effective feeding program for Hanwoo cattle by shortening the feeding period.
Pesticide application in agriculture provides significant benefits such as protection from disease, prevention of harmful insects, and increased crop yields. However, accurate toxicological tests and risk assessments are necessary because of many related adverse effects associated with pesticide use. In this review, we discuss and analyze residual pesticides contained in livestock feed in Korea. A pesticide residue tolerance standard for livestock feed has not been precisely established; so, risk assessments are required to ensure safety. Standards and approaches for animal criteria and appropriate methods for evaluating residual pesticides are discussed and analyzed based on technology related to animal product safety in Korea. The safety of livestock feed containing pesticides is assessed to establish maximum residue limits relative to pesticides. Analysis of residual pesticides in milk, muscle, brain, and fat was performed with a livestock residue test and safety evaluation of the detected pesticide was performed. Efficacy of organic solvent extraction and clean-up of feed was verified, and suitability of the instrument was examined to establish if they are effective, rapid, and safe. This review discussed extensively how pesticide residue tolerance in livestock feed and hazard evaluation may be applied in future studies.
현재 농가에서 이용되고 있는 보광등과 난방등의 특성을 조사하고 하우스내 기상 환경과 절화장미의 개화와 품질에 미치는 영향을 알아보기 위해 실험을 수행하였다. 보광등으로는 LED등(light emitting diode lamp, LED), 메탈할라이드등(metal halide lamp, MH), 고압나트륨등(high pressure sodium lamp, HPS)을, 난방등으로는 나노탄소섬유 적외선등(nano-carbon fiber infrared lamp, NCFI)을 절화장미 재배상 위에 설치하였다. 그 결과 LED와 NCFI의 광도는 각각 6.8μmol·m-2·s-1과 0.4μmol·m-2·s-1으로 매우 낮은 반면, MH와 HPS는 79 ~ 100μmol·m-2·s-1 범위로 높은 편이었다. 또한 LED와 MH는 적외선역에 파장이 없어 발열량이 거의 없었으나, HPS와 NCFI는 적외선역에 파장이 많아 발열이 많은 것으로 나타났다. 동일한 공간에서 칸막이로 실험구간을 나누어 실험이 수행되었음에도 불구하고 MH, HPS, NCFI에 의해 온도는 2°C 정도 상승되는 결과를 얻었으나, 습도에서는 처리간 차이가 보이지 않았다. 광도에 상관없이 모든 전등들은 절화장미의 개화를 촉진시켜 무처리구에 비해 적게는 5 ~ 7일, 많게는 12 ~ 14일 개화가 촉진되는 것으로 나타났다. 광량이 충분한 봄철에는 전조처리에 의해 개화소요일수가 짧아지는 만큼 절화장도 대조구보다 짧아졌지만, 광량이 부족한 겨울철에는 전조처리에 의해 절화장이 길어져 품질이 향상되는 결과를 얻을 수 있었다.
Chrysanthemums (Dendranthema grandiflorum ‘Iwanohakusen’) were grown in a greenhouse with complete nutrient solution system to investigate the effect of silicon (Si) supplement on salt induced deleterious effects in chrysanthemum plants. The experiment was conducted in plastic pots supplemented with a mixture of upland soil : leaf mold : river sand (3:3:4, v:v:v). Si and salinity were treated in combination with two levels of NaCl (0 and 100 mM) and two sources of silicon (K2SiO3, KSi and silicate fertilizer, SiF) at the same concentration (1.8 mM Si) by weekly-drenching for 12 weeks. Chrysanthemum plants supplemented with Si increased in fresh and dry matter enhancing water content and salinity tolerance. The plants grown under salt stress produced less fresh and dry matter than control plant. However, Si supplement to plants under salt stress ameliorated negative effects of salt stress. In soil, EC and NaCl increased by salt stress were mitigated by Si supplement. Salt stress significantly decreased the contents of K and P in leaf, but Si supplement under salt stress significantly recovered the decreased contents with enormous desorption of K and P in soil. Added Si significantly increased content of available SiO2 with its adsorption by salt stress in soil, which was directly related to Si accumulation in leaf. However, Si uptake by roots was suppressed by salt stress irrespective of Si supplement. Si supplement did not ameliorated the negative effects of salt stress on chlorophyll content and membrane integrity in leaf of chrysanthemum plant although significantly increased Si content in leaf, but reversed pest (Liriomyza trifolii) resistance to above-control level.
Ampelopsis brevipedunculata, a porcelain berry that is a kind of Korean domestic wild berry (Viticeae), has been known to be resistant to diseases and insects. A total of 2,622 unigenes containing 912 contigs and 1,710 singletons were obtained by sequencing 5,839 expressed sequence tag (EST) clones derived from the cDNA library of wild grape, A. brevipedunculata. In the gene ontology analysis, 1,175 genes related to biological process, 1,516 genes related to molecular function, and 1,413 ones related to cell components were annotated. Among the genes showing molecular function, 17 clones were classified into defense-related genes, and 102 were known to be related with responses to stress in plants. Domains, such as leucine-rich repeat, Serine/threonine protein kinase-related, WD40 repeat, EF-hand calcium-binding, pentatricopeptide repeat, and pathogenesis-related transcriptional factor were highly expressed. Genes encoding defense related proteins, such as chitinase, catalase, protein-serine/threonine kinases, were also clustered into an abundant group in cDNAs from A. brevipedunculata. Approximately, 80 simple sequence repeats with 2~5 nucleotides were detected in the cDNAs of A. brevipedunculata. These data could provide useful information for the genetic analysis of wild grapevines and in programs for breeding grape cultivars.
The purpose of this study is to look into the effect of a horticultural activity program on career women’s job satisfaction. As the research subject, this study set 37 career women of 6 occupational clusters, such as elementary school teachers, nurses, social workers, care teachers, and police officers in ‘P’ city, and financiers in ‘D’city. The horticultural activity program was applied for about 10 months from June 2013 to April 2014, and for each job group the program was executed once a week and a total of 8 sessions. As a result of the analysis of job satisfaction by conducting horticultural activity by 6 occupational cluster, occupational clusters like an elementary school teacher, and social worker showed a significant improvement in job satisfaction immediately after horticultural activity was conducted, and in 4 weeks after that, all 5 occupational clusters but a care teacher cluster were found not to show a significant difference. It is expected that various horticultural activity programs would provide emotional stability to working women and consequently improve their overall quality of life. As a result of the above research, this study confirmed that improvement in job satisfaction is more effective when a person steadily participates in gardening activity rather than doing gardening activity temporarily. In addition, this study confirmed that gardening activity could be an alternative remedy which provides a positive change to a career woman’s job satisfaction.
본 연구는 장애전담 어린이집에 재원 중인 발달지체유아를 대상으로 요일별 신체리듬을 고려한 원예활동 프로그램이 유아의 적응행동에 미치는 영향에 대해 알아보고자 하였다. 원예활동 대상자는 S장애전담 어린이집에서 4세~6세 발달지체유아 대조군 8명, 실험군 8명이었다. 원예활동 프로그램은 2012년 10월 15일부터 2012년 11월 22일까지 주 3회 30분씩 총 18회 실시하였다. 전체 적응행동 평가 결과 대조군의 경우 실험 전 65.00점에서 실험 후 66.00점으로 1.00점이 향상되었고 실험군은 전체 적응행동 평가 결과 실험 전 66.00점에서 실험 후 85.50점으로 19.50점이 향상되었다. 실험군의 하위항목별 변화를 살펴보면 개념적 적응행동은 원예활동 실시 전 64.00점에서 실시 후 80.75점으로, 사회적 적응행동은 65.50점에서 80.63점으로, 실제적 적응행동은 74.38점에서 95.38점으로 모든 항목이 원예활동 실시 후 유의하게 향상되는 것을 볼 수 있었다. 이상의 연구결과로부터 요일별 신체리듬의 변화와 유아의 흥미와 참여도를 고려하여 설계한 원예활동프로그램이 장애전담 어린이집 내에 재원하고 있는 발달지체유아들의 적응행동 향상에 효과적인 것으로 나타났다.