The early-onset familial Alzheimer's disease (EOFAD/ FAD), the less common type of Alzheimer's disease (AD) currently affects a vast number of individuals worldwide. This type is being inherited as an autosomal dominant fashion. Missense mutations on Amyloid precursor protein (APP) and Presenilins 1 and 2 (PSEN1 & PSEN2) are known as major genetic factors in FAD. Conversely, missense mutations on microtubule-associated protein tau (MAPT) are also thought to involve. Up to date, several triple-transgenic animal models with muted forms of the human APP, PSENs and MAPT have been reported. Compared to other animals, canines are more emotional and their disease signs can be easily diagnosed. This attempt was to develop a triple transgenic canine model for the AD. We have obtained the coding sequences of APP, PSEN1 and MAPT from Dana-Farber/Harvard Cancer Center DNA resource core at HMS and incorporated several common AD mutations. The transgenic construct is composed of hNSE (ENO2) promoter-driven three AD genes fused together with modified 2A sequences. It was transfected into the canine fetal fibroblasts which were then used to perform somatic cell nuclear transfer (SCNT). The viable transgenic embryos were obtained after in vitro culture and the GFP was detected. In this study, we have successfully produced viable triple transgenic canine cloned embryos using SCNT technique. These transgenic canine embryos will be further developed into canines with FAD. The transgenic canines will be a good candidate in the AD research field.

The organic light-emitting diodes are fabricated with six anthracene derivatives containing simple substituents such as phenyl or naphthyl group. The device structure is as in the following: Indium tin oxide (ITO) (180 nm)/4,4-4,4`,4``-tris[N-(1-naphthyl)-Nphenylamino] triphenylamine (2-TNATA) (30 nm)/4,4`-bis[N-(1-naphthyl)-N-phenyl-1-amino] biphenyl (NPB) (20 nm)/Emitting compound (30 nm)/2,2′,2"- (1,3,5-Benzinetriyl)-tris (1-phenyl-1-H–benz-imidazole) TPBi (40 nm)/lithium quinolate (Liq) (2 nm)/Al (100 nm). In the emitting layer the anthracene derivatives are used without any dopant. All the six devices show blue emissions. Among the tested diodes, the one with 9-(2-naphthyl)-10-(p-tolyl) anthracene (2-NTA) exhibited luminous efficiency, power and external quantum efficiencies of 3.26 cd/A, 0.98 lm/A, 2.8 % at 20 mA/cm .

Anthracene has been a motive molecule for the blue-emitting materials in OLED. Since the blue emission needs big band gap between HOMO and LUMO, the blue-emitting materials are rare. In this paper, some anthracene derivatives containing simple aryl groups are synthesized and characterized. Regardless of the substituents the absorption and the emission bands are similar to each other and similar to the derivatives with the bulky silyl groups. The thermal and the CIE tests imply that among the tested 9-(2-naphthyl)-10-phenylanthracene is most promising for the diode. The material for the emission layer has to be investigated, which is simple to be prepared as well as good in the electrical and the thermal properties.

The objective of this study was to determine the growth characteristics of cool (C1) and warm season grasses (C2) in pastures mixed with C1 and C2 at an altitude of 400 m in Jeju island to establishing pasture suitable for grazing horses and to evaluate the effect of pastures mixed with tall and short type grasses on the intake characteristics of horses. C1 used in this study was Kentucky bluegrass, redtop (short type grass) and tall type grasses were orchardgrass and tall fescue, respectively. Treatments of this study were consisted of four groups and the short type grass used in pastures mixed with C1 and C2 was mainly bermudagrass. Four treatment groups were follow as; Treatment 1 (bermudagrass + Kentucky bluegrass + redtop) 2) Treatment 2 (bermudagrass + tall fescue + orchardgrass) 3) Treatment 3 (Kentucky bluegrass + redtop) 4) Treatment 4 (tall fescue + orchardgrass). Bermudagrass was a little winter killing and inhibition of plant growth at an altitude of 400 m. Plant heights in pastures mixed with C1 and C2 were grown better than that in pastures mixed with C1. Especially, plant height in Treatment 4 was higher than other treatments. Dry matter yield was in the following order: Treatment 4> Treatment 3> Treatment 2> Treatment 1. Dry matter yield in pastures mixed with C1 increased as compared with pastures mixed with C1 and C2. Dry matter yield in Treatment 3 was higher than other treatments. In the first investigation regarding vegetation distribution, bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 11.7 and 13.3%, respectively. The growth of bermudagrass in winter was low due to the cold damage. However the growth of Kentucky bluegrass, redtop, tall fescue and orchardgrass was good. In the second investigation, bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 5.0 and 11.7%, respectively. Growth of forage in the second investigation was poor as compared to the first investigation. nutritive values(crude protein content, neutral detergent fiber content, acid detergent fiber content, digestibility) were good in pastures mixed with C1 Especially, nutritive values in pastures mixed with tall was higher than those of pastures mixed short grasses. P content among minerals in Treatment 1 was higher than other groups. However, the content of Ca, Mg and Mn were lower. The contents of Ca, K, Mg, Na, Cu, Zn and Fe in Treatment 2 were higher. However, the contents of K, Mg, Na, Cu, Zn and Fe in Treatment 3 were lower. Therefore, we suggest that cool season grasses with short grasses were sowed to establishing pasture suitable for grazing horses at an altitude of 400 m in Jeju island.

본 연구는 2014년 7월부터 2015년 9월까지 제주 한국마 사회 제주육성목장(해발 450m) 시험포장에서 여름 동안 생산성이 유지될 수 있는 남방형 목초를 도입하여 재배에 이용하기 위하여 Bermudagrass 품종(Cynodon dactylon (L.) Pers. : Giant, Cheyene, Mohawk, Panchero Frio, Common) 과 Bahiagrass 품종(Paspalum notatum : TifQuik, Tifton 9, Argentine), 그리고 Teff grass(Eragrostis tef : Tiffany) 품종 의 생육특성 및 건물 수량을 평가하기 위하여 실시하였다. 실험설계는 품종을 처리로 10처리 3반복 난괴법으로 배치 하였다. 1차년도 1차 수확은 8월 31일, 2차 수확은 9월 21일이며 2차년도 1차 수확은 7월 22일, 2차 수확은 8월 14 일, 3차 수확은 9월 11일로 성장단계를 고려하여 수확하였 다. 1차년도 남방형 품종의 건물 수량은 Teff grass의 Tiffany가 산파와 조파 각각에서 2.59와 2.00Ton/ha로 가 장 높은 건물수량을 나타내었으며, Bermudagrass의 Common이 산파에서 1.21Ton/ha로 다년생 남방형 목초 중 가장 높은 수량을 나타내었다. 2차년도 남방형 품종의 건물 수량은 Bahiagrass의 Tifton9이 산파에서 15.06Ton/ ha로 가장 높은 수량을 나타내었으며 Bahiagrass의 Tifquik이 산파에서 14.68Ton/ha로 두 번째로 높은 수량을 나타내었다. 조단백질 함량은 Bahiagrass의 Argentine이 19.75%로 가장 높게 나타났으며 NDF 함량은 Bermudagrass 의 common이 61.69%로 가장 높은 것으로 나타났다. 한해 에 의한 피해의 경우 대부분의 Bermudagrass 품종의 경우 에 동절기 적응성이 떨어졌으나 Bahiagrass의 Tifton 9 품 종의 경우 한해에 대한 피해를 거의 받지 않았으며 출현, 내병성, 내습성, 도복성은 품종 간 차이가 거의 없었으며 Teff grass의 Tiffany이 도복과 습해에 의한 피해가 나타났다. 이상의 결과를 종합해 볼 때 제주지역에서 Bermudagrass와 Bahiagrass의 일부 품종은 월동할 수 있어 여름철 조사료자 원으로 활용이 가능하지만, 품종 간 내한성 능력과 재생능 력이 다르므로 이를 고려하여 품종을 선택할 필요가 있다.

The objective of this study was to determine the growth characteristics of cool (C1) and warm season grasses (C2) in pastures mixed with C1 and C2 suitable for grazing horses and effect of pastures mixed with tall and short grasses on the intake characteristics of horses. C1 used in this study was Kentucky bluegrass, Redtop (short type grass) and tall type grasses were orchardgrass and tall fescue, respectively. The short type grass used as C2 was Bermudagrass. This study had the following four treatment groups: 1) Treatment 1 (Bermudagrass + Kentucky bluegrass + Redtop) 2) Treatment 2 (Bermudagrass + tall fescue + orchardgrass) 3) Treatment 3 (Kentucky bluegrass + Redtop) 4) Treatment 4 (tall fescue + orchardgrass). There was no winterkilling or lodging problem at an altitude of 200 m. Plant heights in mixed pasture of Treatment 1, 2, 3, and 4 were 53.9, 58.2, 57.5, and 78.1 cm, respectively. Plant height was the highest in Treatment 4. Dry matter yield was in the following order : Treatment 4 > Treatment 3 > Treatment 2 > Treatment 1. In the first investigation regarding vegetation distribution, Bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 70% and 66.7%, respectively. Overall, other grasses showed poor growth. In the second investigation of vegetation distribution, Bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 80% and 60.7%, respectively. Crude protein content, neutral detergent fiber content, acid detergent fiber content, digestibility value, and nutritive values were the lowest in Treatment 4, followed by those in Treatment 1, Treatment 2 and Treatment 3.

본 연구는 오차드그라스와 톨 페스큐 혼파초지에서 비축 시기별(8월 1일, 15일, 30일) 으로 처리하여 11월 중순경(11.10∼11.12)에 수확할 때 목초의 비축시기에 따른 생산량과 목초의 사료 가치 등을 비교하기 위해 수행하였다. 목초 비축시기에 따른 오차드그라스와 톨 페스큐 혼파초지(동량 혼파초지)의 건물수량은 8월하순구가 2,983.0kg/ha으로 가장 높은 경향을 보였다. 목초의 비축시기가 늦어짐에 따라 목초의 생산량도 증가하였으나 유의성이 인정되지는 않았다. 조사료의 조단백질은 16.5∼18.16%으로 목초 비축시기별로 일정한 경향을 나타내지 않았다. NDF와 TDN는 비축시기가 늦을수록 증가하는 경향을 나타내었으나 유의성이 인정되지는 않았다. 반면에 ADF 비율은 목초 비축시기가 감소함에 따라 점차 감소하는 것으로 나타내었으나 유의성이 인정되지는 않았다. 무기물의 변화는 비축 기간이 증가할수록 P, Ca, Mg, Na, Zn이 증가하였고 반면에 Mn, Cu는 감소하는 경향을 나타내었다. 결과적으로 8월 초순부터 초지를 비축할 경우 고온으로 인한 생육 위축으로 목초의 수량증가는 기대하기 어렵다. 따라서 제주도에서 가을철에 활용하기 위한 초지를 비축할 경우에는 8월 하순 이후에 시작하는 것이 바람직할 것으로 사료된다.

The legume family (Fabaceae) is the third largest family of flowering plants worldwide and often damaged by stink bugs, particularly Riptortus clavatus Thunberg (Heteroptera: Alydidae). This insect selectively feeds and oviposits on these plants, resulting in >50% of damage to soybeans harvested in autumn. The most common control methods for this insect are periodic application of chemical pesticides, pheromone traps, and bait crops, but still they encounters residual problem, insect resistance or low control efficacy. The current management system needs alternative control methods, which possibly combined with the present methods. Some entomopathogenic fungi have high virulence against the nymphs and adults of R. clavatus in a favorable conditions, reaching ∼80% mortality with mycosis in 3∼5 days. The genera of Beauveria and Metarhizium are possible control agents for effective and safe management of the serious pest. Fungal infection in R. clavatus was observed using an egfp-expressing fungal transformant. Thermotolerant entomopathogenic fungal conidia can be produced in cereal substrates, which enables conidia to be stable for long times. Our considerations need to be given to the combinations with other control methods, such as chemical pesticides or pheromone trap.

The purpose of this study is to provide the case of influence of myofacial release(MFR) and cryotherapy in acute whiplash injury. The subjects were consisted of 3 adults(2 males, 1 female) who had been diagnosed with whiplash injury. The performance period was 1 weeks, 6 times and 1 time is 40min(MFR: 20min, cryotherapy: 20min). We measured neck pain(Visual analogue scale, VAS) and cervical alignment(cervical curvature & line of gravity). All measurements of each subject were measured at pre-treatment and post-treatment. The results are listed below. The neck pain(VAS) index decreased from 7.54cm to 4.11cm. The cervical curvature increased from 23.62°to 29.76°. The line of gravity decreased from 8.03mm to 3.66mm. Based on the results, it can be suggested that both MFR and cryotherapy can be used to neck pain and alignment in acute whiplash injury.

The fact that flip-flops, one of many different types of unstable shoes, are light and relatively easy to put on, accounts for their popularity among people. But because flip-flops rely heavily on the support of a single thong between your first and second toes, they impose a huge amount of pressure onto lower leg. Thus in the following experiment we tried to examine the different effects of flip-flops and running shoes in terms of their effect on muscle activity and fatigue of tibialis anterior and gastrocnemius during walking. In order to measure an electromyogram we used Free EMG system. 10 men and 10 women in running shoes ran on treadmills for 15 minutes at 4.8km/h, 2 days later the same experiment was carried out, but this time, in flip-flops. p value turned out to be greater than .05 and thus there was no considerable difference between the effects of flip-flops and running shoes on muscle activity and fatigue during walking. Therefore we conclude that despite the fact that flip-flops are considered unstable, their effects on muscle activity and fatigue of tibialis anterior and gastrocnemius are negligible.

This study was carried out to identify how a self-stretching exercise program affects pain for each body area, pain relief and job satisfaction for care workers. 20 of 40 care workers with musculoskeletal symptom were randomly selected and participated a self-stretching exercise program consisting of 15 motions. The intervention was done five times or more per weeks for 8 weeks and 1 session lasted within 15 minutes. 'Musculoskeletal symptom survey table' of the Korea Occupational Safety and Health Agency(KOSHA) and JDI(Job Descriptive Index) was used for pain on the musculoskeletal symptom and job satisfaction. Survey were done twice before and after the program. The result of this study showed that self-stretching exercise program group(SSPG) relieved from pain significantly in the shoulders(p<.01) and lumbar(p<.05), comparing to the non selfstretching exercise program group(NSPG). Although no significant difference on variations in the JDI appeared in SSPG, the significant reduction appeared from the colleague relationship and organization in NSPG(p<.05). SSPG showed the significant increase on variations in JDI from the job and organization comparing to NSPG. Especially, the improvement on satisfaction for the organization was shown(p<.05). Accordingly, the self-stretching exercise program for care workers can be said to positively affect the overall pain relief and increase on the JDI.

The pear psylla, Cacopsylla pyricola Foerster (Homoptera: Psyllidae), is a serious insect pest of commercial pear crops. The species, which resides on pear trees throughout its life cycle, is rapidly spreading in some regions of the world. Given the life cycle, it is unclear how such a rapid spread has been facilitated. Presently, the population genetic structure of the species including genetic diversity and gene flow was studied to understand the nature of dispersal and field ecology of the species. Pear psylla was collected from several pear orchards in Korea. The 658-bp region of mitochondrial COI gene and the 716-bp long complete internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA were sequenced. Unlikely other previously studied insect pests, the COI-based genetic diversity of the pear psylla was extremely low (maximum sequence divergence of 0.15%). This finding allowed us to conclude that the species may have been introduced in Korea relatively recently, possibly with the phenomenon of genetic bottlenecks. ITS2 sequence-based analyses of phylogeny, population differentiation, gene flow, and hierarchical population structure all concordantly suggested that the pear psylla populations in Korea are neither genetically isolated nor hampered for gene flow. These genetic data are concordant with the dispersal of an overwintering winterform morph outside the non-pear habitat in the fall and the possibility of subsequently longer distant dispersal.

This study was to investigate the effects of upper and lower limb composing patterns of PNF(proprioceptive neuromuscular facilitation) on the static balance ability by 20 subjects for 6 weeks. This study was measured left one leg standing and right one leg standing with closed eyes on Good Balance system. These results led us to the conclusion that the mean speed of X, Y direction, COP(center of Pressure) velocity moment showed a statistical decrease when applying post-exercise. The above results from this study indicated that upper and lower limb composing patterns of PNF exercise has improved the static balance ability. As a result, this study showed that upper and lower limb composing patterns exercise improve the ability of balance in young adults. Based on this study, it may be applied to old people.

The purpose of this study is to investigate the effects of closed and open kinetic chain exercise for increasing knee joint function on the knee position sense in the normal adults. Thirty normal adults(male 15, female 15; mean age: 22.13±2.58 years) were participated in this study into two groups, each with 15 people. The group I was trained that closed kinetic chain exercise on the knee joint and the group II was trained that open kinetic chain exercise on the knee joint. Exercise programs performed for 4 weeks, 3 times a week were using Shuttle 2000-1 closed kinetic chain exercise and Knee Extensor open kinetic chain exercise(HUR, Filand). The results of this study were as follows: 1) There were statistically significant decreasing of measuring error degree in 0-20。were found between before and after training in closed kinetic chain exercise(p<.05). 2) There were statistically significant decreasing of measuring error degree in 21-40。were found between before and after training in closed kinetic chain exercise(p<.05). 3) There were statistically significant decreasing of measuring error degree in 41-60。were found between before and after training in closed kinetic chain exercise(p<.05). 4) There were statistically significant decreasing of measuring error degree in 0- 20。were found between before and after training in open kinetic chain exercise(p<.05). 5) There were statistically significant decreasing of measuring error degree in 21-40。were found between before and after training in open kinetic chain exercise(p<.05). 6) There were statistically significant decreasing of measuring error degree in 41- 60。were found between before and after training in open kinetic chain exercise(p<.05). In conclusion, these results suggest that closed and open kinetic chain exercise has increased in the knee joint proprioception between before and after training. Especially, closed kinetic chain exercise could be more useful intervention than open kinetic chain exercise for increasing proprioceptive sense.

This study is intended to examine the motor skill learning and treadmill exercise on motor performance and synaptic plasticity in the cerebellar injured rats by harmaline. Experiment groups were divided into four groups and assigned 15 rats to each group. GroupⅠ was a normal control group(induced by saline); GroupⅡ was a experimental control group(cerebellar injured by harmaline); GroupⅢ was a group of motor skill learning after cerebellar injured by harmaline; GroupⅣ was a group of treadmill exercise after cerebellar injured by harmaline. In motor performance test, the outcome of groupⅡ was significantly lower than the groupⅢ, Ⅳ(especially groupⅢ)(p<.001). In histological finding, the experimental groups were destroy of dendrities and nucleus of cerebellar neurons. GroupⅢ, Ⅳ were decreased in degeneration of cerebellar neurons(especially groupⅢ). In immunohistochemistric response of synaptophysin in cerebellar cortex, experimental groups were decreased than groupⅠ. GroupⅢ's expression of synaptophysin was more increased than groupⅡ, Ⅳ. In electron microscopy finding, the experimental groups were degenerated of Purkinje cell. These result suggest that improved motor performance by motor skill learning after harmaline induced is associated with dynamically altered expression of synaptophysin in cerebellar cortex and that is related with synaptic plasticity.

We have investigated the optical properties of the global haze on Titan from spectra recorded between 7100 and 9200Åwhere CH4 absorption bands of various intensities occur. The Titan spectra were obtained on Feb. 23, 2005 (UT), near the times of the Cassini T3 flyby and Huygens probe, using an optical echelle spectrograph (BOES) on the 1.8-m telescope at Bohyunsan Observatory in Korea. In order to derive the optical properties of the haze as a function of altitude, we developed an inversion radiative-transfer program using an atmospheric model of Titan and laboratory CH4 absorption coefficients available from the literature. The derived extinction coefficients of the haze increase toward the surface, and the coefficients at shorter wavelengths are greater than those at longer wavelengths for the 30 - 120 km altitude range, indicating that the Titanian haze becomes optically thin toward the longer wavelength range. Total optical depths of the haze are estimated to be 1.4 and 1.2 for the 7270 -7360Åand 8940 -9150Å ranges, respectively. Based on the Huygens/DISR data set, Tomasko et al. (2005) reported total optical depths of 2.5 - 3.5 at 8290Å depending on the assumed fractal aggregate particle model. The total optical depths based on our results are smaller than those of Tomasko et al., but they partially overlap with their results if we consider a large uncertainty from possible variations of the CH4 mixing ratio over Titan's disk. We also derived the single scattering albedo of the haze particles as a function of altitude: it is less than 0.5 at altitudes higher than ~150Km the average particle radius is smaller than the wavelengths, whereas near the surface, it becomes comparable or greater.